| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94511-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37251-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97554-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98750-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26233-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-50071-0.1mg | |||
![Western Blot: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Analysis of extracts of various cell lines, using CHRM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Western-Blot-NBP2-94511-img0001.jpg "Western Blot: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Analysis of extracts of various cell lines, using CHRM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Analysis of SH-SY5Y cells using CHRM1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94511-img0004.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Mouse brain using CHRM1 Rabbit pAb at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-Paraffin-NBP2-94511-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Analysis of Neuro-2a cells using CHRM1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94511-img0003.jpg)
![Western Blot: Ets-1 Antibody (8A8) [NBP2-22169] - Western blot analysis using ETS1 mouse mAb against Jurkat (1), HepG2 (2) and ETS1-hIgGFc transfected HEK293 (3) cell lysate.](http://images.novusbio.com/fullsize/Ets-1-Antibody-8A8-Western-Blot-NBP2-22169-img0004.jpg "Western Blot: Ets-1 Antibody (8A8) [NBP2-22169] - Western blot analysis using ETS1 mouse mAb against Jurkat (1), HepG2 (2) and ETS1-hIgGFc transfected HEK293 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Ets-1 Antibody (8A8) [NBP2-22169] - Immunofluorescence analysis of NIH/3T3 cells using anti-ETS1 mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Ets-1-Antibody-8A8-Immunocytochemistry-Immunofluorescence-NBP2-22169-img0002.jpg)
![Immunohistochemistry-Paraffin: Ets-1 Antibody (8A8) [NBP2-22169] - Immunohistochemical analysis of paraffin-embedded human Colon, Adipocytes tissues using anti-ETS1 mouse mAb](http://images.novusbio.com/fullsize/Ets-1-Antibody-8A8-Immunohistochemistry-Paraffin-NBP2-22169-img0003.jpg)
![Flow Cytometry: Ets-1 Antibody (8A8) [NBP2-22169] - Flow cytometric analysis of Jurkat cells using anti-ETS1 mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Ets-1-Antibody-8A8-Flow-Cytometry-NBP2-22169-img0001.jpg)
![Western Blot: Menin Antibody (7D3E10) [NBP2-37251] - Analysis using MEN1 mAb against HEK293 (1) and MEN1 (AA: 392-554)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Menin-Antibody-7D3E10-Western-Blot-NBP2-37251-img0003.jpg "Western Blot: Menin Antibody (7D3E10) [NBP2-37251] - Analysis using MEN1 mAb against HEK293 (1) and MEN1 (AA: 392-554)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: Menin Antibody (7D3E10) [NBP2-37251] - Analysis of rectum cancer tissues using MEN1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Menin-Antibody-7D3E10-Immunohistochemistry-NBP2-37251-img0005.jpg)
![Flow Cytometry: Menin Antibody (7D3E10) [NBP2-37251] - Analysis of Hela cells using MEN1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Menin-Antibody-7D3E10-Flow-Cytometry-NBP2-37251-img0004.jpg)
![Western Blot: Menin Antibody (7D3E10) [NBP2-37251] - Analysis using MEN1 mAb against human MEN1 (AA: 392-554) recombinant protein. (Expected MW is 43.3 kDa)](http://images.novusbio.com/fullsize/Menin-Antibody-7D3E10-Western-Blot-NBP2-37251-img0002.jpg)
![ELISA: Menin Antibody (7D3E10) [NBP2-37251] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Menin-Antibody-7D3E10-ELISA-NBP2-37251-img0001.jpg)
![Western Blot: EEF1A2 Antibody [NBP2-97554] - Anti-EEF1A2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: U-251 MG Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Western-Blot-NBP2-97554-img0005.jpg "Western Blot: EEF1A2 Antibody [NBP2-97554] - Anti-EEF1A2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: U-251 MG Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa")
![Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP2-97554] - Immunochemical staining of human EEF1A2 in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Immunohistochemistry-Paraffin-NBP2-97554-img0004.jpg)
![Immunoprecipitation: EEF1A2 Antibody [NBP2-97554] - EEF1A2 was immunoprecipitated using: Lane A: 0.5 mg U-251MG Whole Cell Lysate 4 ul anti-EEF1A2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-EEF1A2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Immunoprecipitation-NBP2-97554-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: EEF1A2 Antibody [NBP2-97554] - Immunofluorescence staining of EEF1A2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97554 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97554_rabbit-polyclonal-eef1a2-antibody-235202512205533.png)
![Western Blot: Tafazzin/TAZ Antibody [NBP2-98750] - Anti-Tafazzin/TAZ rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 40 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Tafazzin-TAZ-Antibody-Western-Blot-NBP2-98750-img0002.jpg "Western Blot: Tafazzin/TAZ Antibody [NBP2-98750] - Anti-Tafazzin/TAZ rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 40 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: Tafazzin/TAZ Antibody [NBP2-98750] - Immunochemical staining of human Tafazzin/TAZ in human skeletal muscle with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Tafazzin-TAZ-Antibody-Immunohistochemistry-Paraffin-NBP2-98750-img0005.jpg)
![Immunohistochemistry-Paraffin: Tafazzin/TAZ Antibody [NBP2-98750] - Immunochemical staining of human Tafazzin/TAZ in human placenta with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Tafazzin-TAZ-Antibody-Immunohistochemistry-Paraffin-NBP2-98750-img0004.jpg)
![Immunoprecipitation: Tafazzin/TAZ Antibody [NBP2-98750] - Tafazzin/TAZ was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate0.5 ul anti-Tafazzin/TAZ rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-Tafazzin/TAZ rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odyssey technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa](http://images.novusbio.com/fullsize/Tafazzin-TAZ-Antibody-Immunoprecipitation-NBP2-98750-img0001.jpg)
![Immunohistochemistry-Paraffin: Tafazzin/TAZ Antibody [NBP2-98750] - Immunochemical staining of human Tafazzin/TAZ in human kidney with NBP2-98750 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp2-98750_rabbit-polyclonal-tafazzin-taz-antibody-235202514331543.png)
![Western Blot: HSF1 Antibody (JE44-79) [NBP3-32444] - Western blot analysis of HSF1 on different lysates with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lane 4: HEK-293 cell lysate Lane 5: K-562 cell lysate Lane 6: Jurkat cell lysate Lane 7: A549 cell lysate Lane 8: HepG2 cell lysate Lane 9: LoVo cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32444) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-278202415242677.jpg "Western Blot: HSF1 Antibody (JE44-79) [NBP3-32444] - Western blot analysis of HSF1 on different lysates with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lane 4: HEK-293 cell lysate Lane 5: K-562 cell lysate Lane 6: Jurkat cell lysate Lane 7: A549 cell lysate Lane 8: HepG2 cell lysate Lane 9: LoVo cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32444) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HSF1 Antibody (JE44-79) [NBP3-32444] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32444) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-29820241420917.jpg)
![Immunocytochemistry/ Immunofluorescence: HSF1 Antibody (JE44-79) [NBP3-32444] - Immunocytochemistry analysis of HeLa cells labeling HSF1 with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-2920241033401.jpg)
![Flow Cytometry: HSF1 Antibody (JE44-79) [NBP3-32444] - Flow cytometric analysis of HeLa cells labeling HSF1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32444, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-392024884655.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0002.jpg "Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: CTCF Antibody [NBP2-59228] - ChIP was performed with the antibody against CTCF on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the H19 imprinting control region, and a specific region in the GAPDH gene, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat region, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/CTCF-Antibody-Chromatin-Immunoprecipitation-NBP2-59228-img0004.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0001.jpg)
![ELISA: CTCF Antibody [NBP2-59228] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against CTCF. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:500,000.](http://images.novusbio.com/fullsize/CTCF-Antibody-ELISA-NBP2-59228-img0003.jpg)
![Immunofluorescence: CTCF Antibody [NBP2-59228] - HeLa cells were stained with the antibody against CTCF and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the CTCF antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunofluorescence-NBP2-59228-img0005.jpg)
![Western Blot: STAT5A [p Tyr694] Antibody (5F6.F1) [NBP1-77817] - Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4C. For detection Dye light 800 conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 30 min at room temperature](http://images.novusbio.com/fullsize/STAT5A-p-Tyr694-Antibody-5F6-F1-Western-Blot-NBP1-77817-img0002.jpg "Western Blot: STAT5A [p Tyr694] Antibody (5F6.F1) [NBP1-77817] - Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4C. For detection Dye light 800 conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 30 min at room temperature")
![Immunocytochemistry/Immunofluorescence: STAT5A [p Tyr694] Antibody (5F6.F1) [NBP1-77817] - Used at a 1:500 dilution detecting Stat5 in 3T3 cells (immunofluorescent STED microscopy). Red represents Stat5 pY694 protein. Green represents tubulin.](http://images.novusbio.com/fullsize/STAT5A-p-Tyr694-Antibody-5F6-F1-Immunocytochemistry-Immunofluorescence-NBP1-77817-img0006.jpg)
![Immunohistochemistry: STAT5A [p Tyr694] Antibody (5F6.F1) [NBP1-77817] - Analysis of phosphorylated Stat5pY694 in human breast tissue (40X). The antibody was used at a dilution of 1 to 20 ug/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/STAT5A-p-Tyr694-Antibody-5F6-F1-Immunohistochemistry-NBP1-77817-img0005.jpg)
![Western Blot: MED23 Antibody [NB200-339] - Nuclear extract (5 and 25 ug) from HeLa cells. Antibody used at 0.33 ug/ml.](http://images.novusbio.com/fullsize/MED23-Antibody-Western-Blot-NB200-339-img0002.jpg "Western Blot: MED23 Antibody [NB200-339] - Nuclear extract (5 and 25 ug) from HeLa cells. Antibody used at 0.33 ug/ml.")
![Immunocytochemistry/Immunofluorescence: IMP2/IGF2BP2 Antibody [NBP1-33728] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF2BP2 protein stained by IGF2BP2 antibody diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33728-img0007.jpg "Immunocytochemistry/Immunofluorescence: IMP2/IGF2BP2 Antibody [NBP1-33728] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF2BP2 protein stained by IGF2BP2 antibody diluted at 1:500. Blue: Hoechst 33343 staining.")
![Immunohistochemistry-Paraffin: IMP2/IGF2BP2 Antibody [NBP1-33728] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-Immunohistochemistry-Paraffin-NBP1-33728-img0008.jpg)
![Western Blot: IMP2/IGF2BP2 Antibody [NBP1-33728] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IMP2/IGF2BP2 antibody (NBP1-33728) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33728_rabbit-polyclonal-imp2-igf2bp2-antibody-91020242001046.jpg)
![Immunohistochemistry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Image of FIH-1/HIF-1AN Antibody (12F11) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-262202415355734.jpg "Immunohistochemistry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Image of FIH-1/HIF-1AN Antibody (12F11) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Image of FIH-1/HIF-1AN Antibody (12F11) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-272202485636.jpg)
![Western Blot: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Positive Western Blot detected in: Ntera-2 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate. All lanes: FIH-1/HIF-1AN Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 41 kDa Observed band size: 36-53 kDa](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-282202411442647.jpg)
![Immunocytochemistry/Immunofluorescence: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Staining of HepG2 cell with FIH-1/HIF-1AN Antibody (12F11) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 525-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-28220241312243.jpg)
![Flow Cytometry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Overlay Peak curve showing Hela cells stained with FIH-1/HIF-1AN Antibody (12F11) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-282202414312744.jpg)
![Immunohistochemistry-Paraffin: ETS1 associated protein II Antibody (TDP2/1258) [NBP2-50070] - Formalin-paraffin human colon carcinoma stained with TDP2 Mab (TDP2/1258)](http://images.novusbio.com/fullsize/ETS1-associated-protein-II-Antibody-TDP2-1258-Immunohistochemistry-Paraffin-NBP2-50070-img0006.jpg "Immunohistochemistry-Paraffin: ETS1 associated protein II Antibody (TDP2/1258) [NBP2-50070] - Formalin-paraffin human colon carcinoma stained with TDP2 Mab (TDP2/1258)")
![Immunohistochemistry-Paraffin: ETS1 associated protein II Antibody (TDP2/1258) [NBP2-50070] - Formalin-paraffin human Lung Carcinoma stained with TDP2 MAb (TDP2/1258)](http://images.novusbio.com/fullsize/ETS1-associated-protein-II-Antibody-TDP2-1258-Immunohistochemistry-Paraffin-NBP2-50070-img0004.jpg)
![Immunohistochemistry-Paraffin: ETS1 associated protein II Antibody (TDP2/1258) [NBP2-50070] - Formalin-paraffin human Prostate Carcinoma stained with TDP2 MAb (TDP2/1258)](http://images.novusbio.com/fullsize/ETS1-associated-protein-II-Antibody-TDP2-1258-Immunohistochemistry-Paraffin-NBP2-50070-img0003.jpg)
![Immunohistochemistry-Paraffin: ETS1 associated protein II Antibody (TDP2/1258) [NBP2-50070] - Formalin-paraffin human Duodenal carcinoma stained with TDP2 Mab (TDP2/1258)](http://images.novusbio.com/fullsize/ETS1-associated-protein-II-Antibody-TDP2-1258-Immunohistochemistry-Paraffin-NBP2-50070-img0005.jpg)