| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1768SS | |||
| Azide and BSA Free | NBP2-80668 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47427SS | |||
| Azide and BSA Free | NBP2-80865 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22150SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83374-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44419 | |||
| Azide and BSA Free | NBP2-34763-0.1mg | |||
![Western Blot: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of Profilin 1 on Hela cell using anti-Profilin 1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Western-Blot-NBP2-67078-img0008.jpg "Western Blot: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of Profilin 1 on Hela cell using anti-Profilin 1 antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Staining Profilin 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunocytochemistry-Immunofluorescence-NBP2-67078-img0004.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of paraffin-embedded mouse kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunohistochemistry-Paraffin-NBP2-67078-img0007.jpg)
![Flow Cytometry: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of Hela cells with Profilin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Flow-Cytometry-NBP2-67078-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Staining Profilin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunocytochemistry-Immunofluorescence-NBP2-67078-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Staining Profilin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunocytochemistry-Immunofluorescence-NBP2-67078-img0003.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of paraffin-embedded human kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunohistochemistry-Paraffin-NBP2-67078-img0005.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of paraffin-embedded mouse colon tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunohistochemistry-Paraffin-NBP2-67078-img0006.jpg)
![Western Blot: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of SOX9 on NIH/3T3 cell lysates with Rabbit anti-SOX9 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 56 kDa Observed band size: 70 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Western-Blot-NBP2-67690-img0010.jpg "Western Blot: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of SOX9 on NIH/3T3 cell lysates with Rabbit anti-SOX9 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 56 kDa Observed band size: 70 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: SOX9 Antibody (SN74-09) [NBP2-67690] - Staining SOX9 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunocytochemistry-Immunofluorescence-NBP2-67690-img0007.jpg)
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0017.jpg)
![Flow Cytometry: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of Hela cells with SOX9 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Flow-Cytometry-NBP2-67690-img0001.jpg)
![Western Blot: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of SOX9 on SW480 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Western-Blot-NBP2-67690-img0008.jpg)
![Western Blot: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of SOX9 on different lysates with Rabbit anti-SOX9 antibody at 1/500 dilution.Lane 1: SW480 cell lysateLane 2: A431 cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 56 kDaObserved band size: 70 kDaExposure time: 2 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Western-Blot-NBP2-67690-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: SOX9 Antibody (SN74-09) [NBP2-67690] - Staining SOX9 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunocytochemistry-Immunofluorescence-NBP2-67690-img0002.jpg)
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-SOX9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0003.jpg)
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human tonsil tissue using anti-SOX9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0004.jpg)
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded mouse colon tissue using anti-SOX9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0005.jpg)
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded mouse spleen tissue using anti-SOX9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0006.jpg)
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0011.jpg)
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human thyroid tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0012.jpg)
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human breast tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0013.jpg)
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human small intestine tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0014.jpg)
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0015.jpg)
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0016.jpg)
![Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using Chk1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Chk1 cDNA (2).](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Western-Blot-NB100-1768-img0003.jpg "Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using Chk1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Chk1 cDNA (2).")
![Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2G1D5) [NB100-1768] - Chk1 antibody was tested at 1:10 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Immunocytochemistry-Immunofluorescence-NB100-1768-img0005.jpg)
![Immunohistochemistry-Paraffin: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis of FFPE human lung using Chk1 antibody (clone 2G1D5) at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic and nuclear staining of Chk1 was observed as this is a ubiquitous cell checkpoint protein. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Immunohistochemistry-Paraffin-NB100-1768-img0006.jpg)
![Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using anti-Chk1 mouse mAb against A431 (1), Hela (2), NIH/3T3 (3) and K562 (4) cell lysates.](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Western-Blot-NB100-1768-img0002.jpg)
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody [NBP2-20759] - Immunohistochemical analysis of paraffin-embedded U251 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Immunohistochemistry-Paraffin-NBP2-20759-img0006.jpg "Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody [NBP2-20759] - Immunohistochemical analysis of paraffin-embedded U251 xenograft, using antibody at 1:500 dilution.")
![Western Blot: Tubulin alpha-1B Antibody [NBP2-20759] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody (NBP2-20759) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20759_rabbit-polyclonal-tubulin-alpha-1b-antibody-910202420131270.jpg)
![Western Blot: Tubulin alpha-1B Antibody [NBP2-20759] - Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody (NBP2-20759) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20759_rabbit-polyclonal-tubulin-alpha-1b-antibody-91020242063349.jpg)
![Immunohistochemistry: Tubulin alpha-1B Antibody [NBP2-20759] - Tubulin alpha-1B antibody detects Tubulin alpha-1B protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. Tubulin alpha-1B antibody (NBP2-20759) dilution: 1:200.](http://images.novusbio.com/fullsize/nbp2-20759_rabbit-polyclonal-tubulin-alpha-1b-antibody-910202420134043.jpg)
![Immunocytochemistry/ Immunofluorescence: Tubulin alpha-1B Antibody [NBP2-20759] - Tubulin alpha-1B antibody detects Tubulin alpha-1B protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tubulin alpha-1B protein stained by Tubulin alpha-1B antibody (NBP2-20759) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-20759_rabbit-polyclonal-tubulin-alpha-1b-antibody-91020242015728.jpg)
![Immunohistochemistry: Tubulin alpha-1B Antibody [NBP2-20759] - Immunohistochemical analysis (whole mount) of zebrafish embryo, using Tubulin alpha-1B antibody (NBP2-20759) at 1:200 dilution.](http://images.novusbio.com/fullsize/nbp2-20759_rabbit-polyclonal-tubulin-alpha-1b-antibody-91020242021922.jpg)
![Western Blot: Tubulin alpha-1B Antibody [NBP2-20759] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody (NBP2-20759) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20759_rabbit-polyclonal-tubulin-alpha-1b-antibody-910202419593593.jpg)
![Immunohistochemistry: Tubulin alpha-1B Antibody [NBP2-20759] - Tubulin alpha-1B antibody detects Tubulin alpha-1B protein on zebrafish by whole mount immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.Tubulin alpha-1B antibody (NBP2-20759) dilution: 1:200.](http://images.novusbio.com/fullsize/nbp2-20759_rabbit-polyclonal-tubulin-alpha-1b-antibody-910202420131235.jpg)
![Western Blot: MAD2L1 Antibody [NBP1-31311] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0021.jpg "Western Blot: MAD2L1 Antibody [NBP1-31311] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: MAD2L1 Antibody [NBP1-31311] - Analysis of human osteosarcoma cell line U2OS, using MAD2L1 antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31311-img0008.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Human A549 xenograft . MAD2L1 antibody [C2C3], C-term diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0013.jpg)
![Western Blot: MAD2L1 Antibody [NBP1-31311] - A:Hep G2 B:MOLT4 C:Raji 12% SDS PAGE NBP1-31311 diluted at 1:1000](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MAD2L1 Antibody [NBP1-31311] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0019.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - DLD-1 xenograft, using Mad2L1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0009.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Rat fore brain. MAD2L1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0012.jpg)
![Immunoprecipitation: MAD2L1 Antibody [NBP1-31311] - MAD2L1 protein from 293T whole cell extracts using 5 ug of MAD2L1 antibody [C2C3], C-term (NBP1-31311). Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (NBP1-31311). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunoprecipitation-NBP1-31311-img0017.jpg)
![Western Blot: PUM2 Antibody [NB100-387] - Functionality of products expressed from miRNA134. 1, 2 and miRNA99a.1. miR-134 expressed from the miRNA134.1 construct affects the Pum2 protein levels. 10-11 DIV hippocampal neurons were transduced with purified rAAV carrying the indicated plasmids. Eight days later whole cell extracts were prepared and subjected to western blot analysis probing with Pum2 antibody and a beta-actin antibody as loading control. Here we show one out of three representative blots, on average displaying a significant 64% reduction in Pum2 protein levels. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/neuro.04.016.2009/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PUM2-Antibody-Western-Blot-NB100-387-img0003.jpg "Western Blot: PUM2 Antibody [NB100-387] - Functionality of products expressed from miRNA134. 1, 2 and miRNA99a.1. miR-134 expressed from the miRNA134.1 construct affects the Pum2 protein levels. 10-11 DIV hippocampal neurons were transduced with purified rAAV carrying the indicated plasmids. Eight days later whole cell extracts were prepared and subjected to western blot analysis probing with Pum2 antibody and a beta-actin antibody as loading control. Here we show one out of three representative blots, on average displaying a significant 64% reduction in Pum2 protein levels. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/neuro.04.016.2009/abstract), licensed under a CC-BY license.")
![Western Blot: PUM2 Antibody [NB100-387] - Detection of Human and Mouse Pumilio 2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Pumilio 2 antibody NB100-387 used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/PUM2-Antibody-Western-Blot-NB100-387-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Nanog Antibody (1E6C4) [NBP1-47427] - Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using anti-Nanog Mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.](http://images.novusbio.com/fullsize/Nanog-Antibody-1E6C4-Immunocytochemistry-Immunofluorescence-NBP1-47427-img0003.jpg "Immunocytochemistry/Immunofluorescence: Nanog Antibody (1E6C4) [NBP1-47427] - Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using anti-Nanog Mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.")
![Western Blot: Nanog Antibody (1E6C4) [NBP1-47427] - Western blot analysis using anti-Nanog monoclonal antibody against NTERA-2 cell lysate (lane 2).](http://images.novusbio.com/fullsize/Nanog-Antibody-1E6C4-Western-Blot-NBP1-47427-img0004.jpg)
![Western Blot: Nanog Antibody (1E6C4) [NBP1-47427] - Whole cell protein from NTERA-2 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/ml anti-Nanog in 1% milk. Protein was detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Nanog-Antibody-1E6C4-Western-Blot-NBP1-47427-img0005.jpg)
![Western Blot: PDX1 Antibody [NBP2-22150] - WB analysis of PDX1 in INS1 cell lysate.](http://images.novusbio.com/fullsize/PDX1-Antibody-Western-Blot-NBP2-22150-img0003.jpg "Western Blot: PDX1 Antibody [NBP2-22150] - WB analysis of PDX1 in INS1 cell lysate.")
![Immunocytochemistry/Immunofluorescence: PDX1 Antibody [NBP2-22150] - PDX1 antibody was tested in INS1 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PDX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22150-img0001.jpg)
![Immunohistochemistry: PDX1 Antibody [NBP2-22150] - IHC analysis of PDX1 in mouse pancreas.](http://images.novusbio.com/fullsize/PDX1-Antibody-Immunohistochemistry-NBP2-22150-img0002.jpg)
![Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Rat stomach. NFYB antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/NFYB-Antibody-Immunohistochemistry-Paraffin-NBP2-19535-img0005.jpg "Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Rat stomach. NFYB antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Mouse intestine. NFYB antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/NFYB-Antibody-Immunohistochemistry-Paraffin-NBP2-19535-img0004.jpg)
![Western Blot: NFYB Antibody [NBP2-19535] - Various tissue extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (NBP2-19535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-19535_rabbit-polyclonal-nfyb-antibody-91020242063327.jpg)
![Immunohistochemistry: NFYB Antibody [NBP2-19535] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using NFYB antibody (NBP2-19535) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-19535_rabbit-polyclonal-nfyb-antibody-9102024202196.jpg)
![Western Blot: NFYB Antibody [NBP2-19535] - HeLa whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (NBP2-19535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19535_rabbit-polyclonal-nfyb-antibody-91020242041218.jpg)
![Western Blot: Enolase 1 Antibody [NBP1-31324] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP1-31324-img0012.jpg "Western Blot: Enolase 1 Antibody [NBP1-31324] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP1-31324] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ENO1 protein stained by ENO1 antibody [N3C3] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31324-img0008.jpg)
![Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP1-31324] - Paraffin-embedded gastric tissue, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunohistochemistry-Paraffin-NBP1-31324-img0003.jpg)
![Western Blot: Enolase 1 Antibody [NBP1-31324] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP1-31324-img0004.jpg)
![Western Blot: Enolase 1 Antibody [NBP1-31324] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP1-31324-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Enolase 1 Antibody [NBP1-31324] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3].](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP1-31324-img0007.jpg)
![Western Blot: Enolase 1 Antibody [NBP1-31324] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP1-31324-img0010.jpg)
![Western Blot: Enolase 1 Antibody [NBP1-31324] - Sample (30 ug of whole cell lysate) A: zebrafish eye 10% SDS PAGE NBP1-31324 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-31324_rabbit-polyclonal-enolase-1-antibody-910202420134090.jpg)
![Immunohistochemistry: Enolase 1 Antibody [NBP1-31324] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Enolase 1 antibody [N3C3] (NBP1-31324) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-31324_rabbit-polyclonal-enolase-1-antibody-91020242041252.jpg)
![Immunohistochemistry: Enolase 1 Antibody [NBP1-31324] - Enolase 1 antibody [N3C3] detects Eno1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. Enolase 1 antibody [N3C3] (NBP1-31324) dilution: 1:100.](http://images.novusbio.com/fullsize/nbp1-31324_rabbit-polyclonal-enolase-1-antibody-910202420134054.jpg)
![Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374] - Analysis in human placenta and skeletal muscle tissues. Corresponding GLDC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GLDC-Antibody-Immunohistochemistry-Paraffin-NBP1-83374-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374] - Analysis in human placenta and skeletal muscle tissues. Corresponding GLDC RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GLDC Antibody [NBP1-83374] - Western blot analysis in human cell lines Caco-2 and HeLa. Corresponding GLDC RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/GLDC-Antibody-Western-Blot-NBP1-83374-img0014.jpg)
![Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/GLDC-Antibody-Immunohistochemistry-Paraffin-NBP1-83374-img0018.jpg)
![Western Blot: GLDC Antibody [NBP1-83374] - Western blot analysis in mouse liver tissue and rat liver tissue.](http://images.novusbio.com/fullsize/GLDC-Antibody-Western-Blot-NBP1-83374-img0012.jpg)
![Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374] - Staining of human placenta shows cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/GLDC-Antibody-Immunohistochemistry-Paraffin-NBP1-83374-img0009.jpg)
![Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374] - Staining of human kidney shows moderate granular positivity in cytoplasm in cells in tubules.](http://images.novusbio.com/fullsize/GLDC-Antibody-Immunohistochemistry-Paraffin-NBP1-83374-img0016.jpg)
![Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374] - Staining of human liver shows strong granular positivity in cytoplasm in hepatocytes.](http://images.novusbio.com/fullsize/GLDC-Antibody-Immunohistochemistry-Paraffin-NBP1-83374-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Bax Antibody (SPM336) [NBP2-32809] - Immunofluorescence Analysis of Jurkat cells labeling BAX with Bax Antibody (SPM336) followed by Goat anti-Mouse IgG-CF488 (Green).The nuclear counterstain is NucSpot .](http://images.novusbio.com/fullsize/Bax-Antibody-SPM336-Immunocytochemistry-Immunofluorescence-NBP2-32809-img0003.jpg "Immunocytochemistry/Immunofluorescence: Bax Antibody (SPM336) [NBP2-32809] - Immunofluorescence Analysis of Jurkat cells labeling BAX with Bax Antibody (SPM336) followed by Goat anti-Mouse IgG-CF488 (Green).The nuclear counterstain is NucSpot .")
![Immunohistochemistry-Paraffin: Bax Antibody (SPM336) [NBP2-32809] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with Bax Antibody (SPM336).](http://images.novusbio.com/fullsize/Bax-Antibody-SPM336-Immunohistochemistry-Paraffin-NBP2-32809-img0004.jpg)
![Flow Cytometry: Bax Antibody (SPM336) [NBP2-32809] - Flow Cytometric Analysis of human Jurkat cells using Bax Antibody (SPM336).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Bax-Antibody-SPM336-Flow-Cytometry-NBP2-32809-img0002.jpg)