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![Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NB110-40660-img0002.jpg "Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB110-40660] - Staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes. Antibody at 5 ug/mL. Data kindly provided by CMRB, Center of Regenerative Medicine in Barcelona, Spain.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40660-img0001.jpg)
![Western Blot: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Western-Blot-NBP2-73034-img0002.jpg "Western Blot: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO.")
![Immunocytochemistry/Immunofluorescence: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73034-img0004.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Human Ovary tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0009.jpg)
![Flow Cytometry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-NONO antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73034-img0003.jpg)
![Western Blot: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Western-Blot-NBP2-73034-img0001.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0005.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0006.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0007.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0008.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Human prostate tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0010.jpg)
![Western Blot: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NLN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NLN.](http://images.novusbio.com/fullsize/Neurolysin-Antibody-OTI1D6-Azide-and-BSA-Free-Western-Blot-NBP2-72961-img0002.jpg "Western Blot: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NLN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NLN.")
![Immunohistochemistry: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NLN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Neurolysin-Antibody-OTI1D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72961-img0007.jpg)
![Flow Cytometry: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NLN.](http://images.novusbio.com/fullsize/Neurolysin-Antibody-OTI1D6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72961-img0004.jpg)
![Western Blot: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Analysis of extracts (35ug) from 9 different cell lines by using anti-NLN monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Neurolysin-Antibody-OTI1D6-Azide-and-BSA-Free-Western-Blot-NBP2-72961-img0001.jpg)
![Immunohistochemistry: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NLN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Neurolysin-Antibody-OTI1D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72961-img0005.jpg)
![Immunohistochemistry: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NLN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Neurolysin-Antibody-OTI1D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72961-img0006.jpg)
![Immunohistochemistry: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Staining of paraffin-embedded Human tonsil using anti-NLN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Neurolysin-Antibody-OTI1D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72961-img0008.jpg)
![Flow Cytometry: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Analysis of Jurkat cells, using anti-NLN antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Neurolysin-Antibody-OTI1D6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72961-img0003.jpg)
![Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Profilin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Profilin 1.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Western-Blot-NBP2-73605-img0004.jpg "Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Profilin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Profilin 1.")
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Profilin 1.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73605-img0008.jpg)
![Immunohistochemistry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Staining of paraffin-embedded Human Kidney tissue using anti-Profilin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73605-img0009.jpg)
![Flow Cytometry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of Jurkat cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73605-img0007.jpg)
![Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of extracts (10ug) from 10 Human tissue by using anti-PFN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Western-Blot-NBP2-73605-img0001.jpg)
![Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PFN1 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Western-Blot-NBP2-73605-img0002.jpg)
![Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Profilin 1 monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Western-Blot-NBP2-73605-img0003.jpg)
![Immunohistochemistry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Staining of paraffin-embedded Human pancreas tissue using anti-Profilin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73605-img0010.jpg)
![Flow Cytometry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of Hela cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73605-img0005.jpg)
![Flow Cytometry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Profilin 1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73605-img0006.jpg)
![Western Blot: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Western-Blot-NBP2-71363-img0002.jpg "Western Blot: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP.")
![Immunocytochemistry/Immunofluorescence: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CLPP.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71363-img0004.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Human Kidney tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0010.jpg)
![Flow Cytometry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-CLPP antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71363-img0003.jpg)
![Western Blot: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Analysis of extracts (35ug) from 9 different cell lines by using anti-CLPP monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Western-Blot-NBP2-71363-img0001.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0005.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0006.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0007.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0008.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0009.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Human liver tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0011.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of GSK-3 alpha/beta [p Tyr216, p Tyr279] on mouse brain tissue lysates with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279] antibody at 1:500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 46/51 kDa Observed band size: 46/51 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1:500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0013.jpg "Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of GSK-3 alpha/beta [p Tyr216, p Tyr279] on mouse brain tissue lysates with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279] antibody at 1:500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 46/51 kDa Observed band size: 46/51 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1:500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0003.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-Phospho-GSK3 alpha/beta [p Tyr216, p Tyr279) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0017.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on mouse cerebellum tissue lysates. Lane 1: mouse cerebellum tissue, whole cell lysate, 20ug/laneLane 2: mouse cerebellum tissue treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 20ug/lane All lanes : Anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 47/51 kDaObserved band size: 47/51 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0010.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on 293T cell lysates.Lane 1: 293T cells, whole cell lysate, 10ug/lane Lane 2: 293T cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes :Anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 47/51 kDaObserved band size: 47/51 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0011.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: A431 cell lysate Lane 2: Hela cell lysate](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0002.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0004.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0005.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0006.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-GSK-3 alpha/beta (p Tyr216+Y279) antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0014.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0015.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-GSK3 alpha/beta [p Tyr216, p Tyr279] antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0016.jpg)
![Western Blot: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Analysis of p75 NGF Receptor on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-SA39-02-Western-Blot-NBP2-67296-img0011.jpg "Western Blot: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Analysis of p75 NGF Receptor on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Staining NGFR p75 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-SA39-02-Immunocytochemistry-Immunofluorescence-NBP2-67296-img0005.jpg)
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Rat uterus tissue using anti-p75 NGF Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-SA39-02-Immunohistochemistry-Paraffin-NBP2-67296-img0009.jpg)
![Western Blot: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Western blot analysis of NGFR/TNFRSF16/p75NTR on skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperatur](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-SA39-02-Western-Blot-NBP2-67296-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Staining NGFR p75 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-SA39-02-Immunocytochemistry-Immunofluorescence-NBP2-67296-img0001.jpg)
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Analysis of mouse uterus tissue using anti-p75 NGF Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-SA39-02-Immunohistochemistry-Paraffin-NBP2-67296-img0006.jpg)
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Human tonsil tissue using anti-p75 NGF Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-SA39-02-Immunohistochemistry-Paraffin-NBP2-67296-img0007.jpg)
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Human uterus tissue using anti-p75 NGF Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-SA39-02-Immunohistochemistry-Paraffin-NBP2-67296-img0008.jpg)
![Western Blot: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of Profilin 1 on Hela cell using anti-Profilin 1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Western-Blot-NBP2-67078-img0008.jpg "Western Blot: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of Profilin 1 on Hela cell using anti-Profilin 1 antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Staining Profilin 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunocytochemistry-Immunofluorescence-NBP2-67078-img0004.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of paraffin-embedded mouse kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunohistochemistry-Paraffin-NBP2-67078-img0007.jpg)
![Flow Cytometry: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of Hela cells with Profilin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Flow-Cytometry-NBP2-67078-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Staining Profilin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunocytochemistry-Immunofluorescence-NBP2-67078-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Staining Profilin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunocytochemistry-Immunofluorescence-NBP2-67078-img0003.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of paraffin-embedded human kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunohistochemistry-Paraffin-NBP2-67078-img0005.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of paraffin-embedded mouse colon tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunohistochemistry-Paraffin-NBP2-67078-img0006.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human lymphoma with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.](http://images.novusbio.com/fullsize/CD45-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89218-img0002.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human lymphoma with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.")
![Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human spleen with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.](http://images.novusbio.com/fullsize/CD45-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89218-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Staining smooth muscle Myosin heavy chain 11 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunocytochemistry-Immunofluorescence-NBP2-66967-img0002.jpg "Immunocytochemistry/Immunofluorescence: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Staining smooth muscle Myosin heavy chain 11 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded rat trachea tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunohistochemistry-Paraffin-NBP2-66967-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Staining smooth muscle Myosin heavy chain 11 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunocytochemistry-Immunofluorescence-NBP2-66967-img0001.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded human colon tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunohistochemistry-Paraffin-NBP2-66967-img0003.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded human jejunum tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunohistochemistry-Paraffin-NBP2-66967-img0004.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded human prostate tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunohistochemistry-Paraffin-NBP2-66967-img0005.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded human stomach cancer tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunohistochemistry-Paraffin-NBP2-66967-img0006.jpg)
![Western Blot: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of Hes1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysate Lane 2: SK-Br-3 cell lysate](http://images.novusbio.com/fullsize/HES-1-Antibody-SC06-21-Western-Blot-NBP2-67642-img0005.jpg "Western Blot: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of Hes1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysate Lane 2: SK-Br-3 cell lysate")
![Immunocytochemistry/Immunofluorescence: HES-1 Antibody (SC06-21) [NBP2-67642] - Staining Hes1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/HES-1-Antibody-SC06-21-Immunocytochemistry-Immunofluorescence-NBP2-67642-img0003.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-HES-1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/HES-1-Antibody-SC06-21-Immunohistochemistry-Paraffin-NBP2-67642-img0007.jpg)
![Flow Cytometry: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of sh-sy-5y cells with Hes1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/HES-1-Antibody-SC06-21-Flow-Cytometry-NBP2-67642-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HES-1 Antibody (SC06-21) [NBP2-67642] - Staining Hes1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/HES-1-Antibody-SC06-21-Immunocytochemistry-Immunofluorescence-NBP2-67642-img0002.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of paraffin-embedded mouse brain tissue using anti-Hes1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HES-1-Antibody-SC06-21-Immunohistochemistry-Paraffin-NBP2-67642-img0004.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of paraffin-embedded rat brain tissue using anti-Hes1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/HES-1-Antibody-SC06-21-Immunohistochemistry-Paraffin-NBP2-67642-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in RH-35 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0009.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in RH-35 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Immunofluorescence analysis of paraffin-embedded human stomach cancer tissue labeling alpha-smooth muscle Actin (washed with PBS. And then probed with the primary antibody alpha smooth muscle Actin (red) at 1/1,000 dilution and ALDH2 (green) at 1/100 dilution overnight at 4 , washed with PBS. iFluor(TM) 488 conjugate-Goat anti-Mouse IgG and iFluor(TM) 647 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0012.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of Jurkat cells with alpha-Smooth Muscle Actin Antibody (SY02-64) at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Flow-Cytometry-NBP2-67440-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in A431 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in A549 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in HepG2 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human gastric carcinoma tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0005.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human liver tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0006.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human tonsil tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0007.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Immunofluorescence analysis of paraffin-embedded human Kidney tissue labeling alpha smooth muscle Actin (washed with PBS. And then probed with the primary antibody alpha smooth muscle Actin (red) at 1/1,000 dilution overnight at 4 , washed with PBS. iFluor(TM) 647 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0011.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of alpha smooth muscle Actin on different lysates with Rabbit anti-alpha smooth muscle Actin antibody at 1/1,000 dilution.Lane 1: Hela-si NT cell lysate (10 ug/Lane)Lane 2: Hela-si alpha smooth muscle Actin cell lysate (10 ug/Lane)Predicted band size: 42 kDaObserved band size: 42 kDaExposure time: 5 seconds;4-20% SDS-PAGE gel. was shown to specifically react with alpha smooth muscle Actin in Hela-si NT cells. Weakened band was observed when Hela-si alpha smooth muscle Actin sample was tested. Hela-si NT and Hela-si alpha smooth muscle Actin samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67440_rabbit-monoclonal-alpha-smooth-muscle-actin-antibody-sy02-64-western-blot-2372024113558..jpg)