| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86941-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85769-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80894-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85741-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84509-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87406-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88591-25ul | |||
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)
![Western Blot: STI1 Antibody (2E11) [H00010963-M35] - Analysis of STIP1 expression in human ovarian cancer.](http://images.novusbio.com/fullsize/STI1-Antibody-2E11-Western-Blot-H00010963-M35-img0006.jpg "Western Blot: STI1 Antibody (2E11) [H00010963-M35] - Analysis of STIP1 expression in human ovarian cancer.")
![Immunocytochemistry/Immunofluorescence: STI1 Antibody (2E11) [H00010963-M35] - Analysis of monoclonal antibody to STIP1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/STI1-Antibody-2E11-Immunocytochemistry-Immunofluorescence-H00010963-M35-img0002.jpg)
![Immunohistochemistry-Paraffin: STI1 Antibody (2E11) [H00010963-M35] - Analysis of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human ovarian cancer. Antibody concentration 0.75 ug/ml](http://images.novusbio.com/fullsize/STI1-Antibody-2E11-Immunohistochemistry-Paraffin-H00010963-M35-img0003.jpg)
![Flow Cytometry: STI1 Antibody (2E11) [H00010963-M35] - Analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained HeLa cells (Black) as negative control.](http://images.novusbio.com/fullsize/STI1-Antibody-2E11-Flow-Cytometry-H00010963-M35-img0001.jpg)
![Western Blot: STI1 Antibody (2E11) [H00010963-M35] - Analysis of STIP1 expression in MCF-7.](http://images.novusbio.com/fullsize/STI1-Antibody-2E11-Western-Blot-H00010963-M35-img0005.jpg)
![Western Blot: STI1 Antibody (2E11) [H00010963-M35] - Analysis of STIP1 expression in transfected 293T cell line by STIP1 monoclonal antibody (M35), clone 2E11. Lane 1: STIP1 transfected lysate (Predicted MW: 62.6 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/STI1-Antibody-2E11-Western-Blot-H00010963-M35-img0007.jpg)
![Sandwich ELISA: STI1 Antibody (2E11) [H00010963-M35] - Detection limit for recombinant GST tagged STIP1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/STI1-Antibody-2E11-Sandwich-ELISA-H00010963-M35-img0004.jpg)
![Western Blot: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of THY1 on different lysates using anti-THY1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: HUVEC](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Western-Blot-NBP2-66763-img0007.jpg "Western Blot: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of THY1 on different lysates using anti-THY1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: HUVEC")
![Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Staining THY1 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunocytochemistry-Immunofluorescence-NBP2-66763-img0008.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded mouse hippocampus tissue using anti-CD90/Thy1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Staining THY1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunocytochemistry-Immunofluorescence-NBP2-66763-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Staining THY1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunocytochemistry-Immunofluorescence-NBP2-66763-img0002.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded human kidney tissue using anti-THY1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0003.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded human lung cancer tissue using anti-THY1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0004.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded human spleen tissue using anti-THY1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0005.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded human tonsil tissue using anti-THY1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0006.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded mouse brain tissue using anti-CD90/Thy1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0009.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 95 kDa Observed band size: 140/180 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0009.jpg "Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 95 kDa Observed band size: 140/180 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0007.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-NCAM-1/CD56 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0011.jpg)
![Flow Cytometry: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of SH-SY-5Y cells with NCAM antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Flow-Cytometry-NBP2-66968-img0001.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM on different lysates using anti-NCAM antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: Human brain](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0006.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on SH-SY5Y cell lysates with Rabbit anti-NCAM-1/CD56 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 95 kDa Observed band size: 120/140 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0003.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human tonsil tissue using anti-NCAM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0004.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded zebrafish kidney tissue using anti-NCAM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0005.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human tonsil tissue using anti-NCAM-1/CD56 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0010.jpg)
![Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates with Rabbit anti-Lamin B1 antibody (10 ug/Lane Lane 2: PC-12 cell lysate, 10 ug/Lane Lane 3: Mouse liver tissue lysate, 20 ug/Lane Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Western-Blot-NBP2-67405-img0008.jpg "Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates with Rabbit anti-Lamin B1 antibody (10 ug/Lane Lane 2: PC-12 cell lysate, 10 ug/Lane Lane 3: Mouse liver tissue lysate, 20 ug/Lane Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of HepG2 cells labeling Lamin B1 with Rabbit anti-Lamin B1 antibody permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lamin B1 antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunocytochemistry-Immunofluorescence-NBP2-67405-img0009.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0012.jpg)
![Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysateLane 2: Jurkat cell lysate](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Western-Blot-NBP2-67405-img0006.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0001.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human colon cancer tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0002.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human liver tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded mouse colon tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0005.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Lamin B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0010.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: TIMM44 Antibody [NBP1-86941] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TIMM44-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86941-img0010.jpg "Immunocytochemistry/Immunofluorescence: TIMM44 Antibody [NBP1-86941] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: TIMM44 Antibody [NBP1-86941] - Staining of human gastrointestinal shows moderate to strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TIMM44-Antibody-Immunohistochemistry-Paraffin-NBP1-86941-img0017.jpg)
![Immunohistochemistry-Paraffin: TIMM44 Antibody [NBP1-86941] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/TIMM44-Antibody-Immunohistochemistry-Paraffin-NBP1-86941-img0011.jpg)
![Immunohistochemistry-Paraffin: TIMM44 Antibody [NBP1-86941] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/TIMM44-Antibody-Immunohistochemistry-Paraffin-NBP1-86941-img0012.jpg)
![Immunohistochemistry-Paraffin: TIMM44 Antibody [NBP1-86941] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/TIMM44-Antibody-Immunohistochemistry-Paraffin-NBP1-86941-img0013.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: TIMM44 Antibody [NBP1-86941] - Analysis in human cell lines PC-3 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/TIMM44-Antibody-Western-Blot-NBP1-86941-img0014.jpg)
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Staining in human spleen and skeletal muscle tissues using NBP1-85769 antibody. Corresponding NGFR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-Immunohistochemistry-Paraffin-NBP1-85769-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Staining in human spleen and skeletal muscle tissues using NBP1-85769 antibody. Corresponding NGFR RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-Western-Blot-NBP1-85769-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85769-img0007.jpg)
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Staining of human lymph node shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-Immunohistochemistry-Paraffin-NBP1-85769-img0014.jpg)
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-Immunohistochemistry-Paraffin-NBP1-85769-img0015.jpg)
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-Immunohistochemistry-Paraffin-NBP1-85769-img0016.jpg)
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Staining of human spleen shows strong membranous positivity.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-Immunohistochemistry-Paraffin-NBP1-85769-img0017.jpg)
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Staining of human testis shows strong membranous positivity in peritubular myoid cells.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-Immunohistochemistry-Paraffin-NBP1-85769-img0018.jpg)
![Western Blot: PLZF Antibody [NBP1-80894] - Analysis in human cell line HELA.](http://images.novusbio.com/fullsize/PLZF-Antibody-Western-Blot-NBP1-80894-img0007.jpg "Western Blot: PLZF Antibody [NBP1-80894] - Analysis in human cell line HELA.")
![Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894] - Staining of human Pancreas shows weak nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/PLZF-Antibody-Immunohistochemistry-Paraffin-NBP1-80894-img0014.jpg)
![Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894] - Staining of human testis shows moderate to strong positivity in nuclear membrane in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PLZF-Antibody-Immunohistochemistry-Paraffin-NBP1-80894-img0009.jpg)
![Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894] - Staining of human Cerebral cortex shows moderate nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/PLZF-Antibody-Immunohistochemistry-Paraffin-NBP1-80894-img0012.jpg)
![Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894] - Staining of human Lung shows moderate nuclear positivity in macrophages.](http://images.novusbio.com/fullsize/PLZF-Antibody-Immunohistochemistry-Paraffin-NBP1-80894-img0013.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Analysis in human placenta and pancreas tissues. Corresponding TfR (Transferrin R) RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Analysis in human placenta and pancreas tissues. Corresponding TfR (Transferrin R) RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human cell line U-251 MG shows localization to endosomes and lysosomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85741-img0014.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0019.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0016.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0017.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: SAMM50 Antibody [NBP1-84509] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SAMM50-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84509-img0012.jpg "Immunocytochemistry/Immunofluorescence: SAMM50 Antibody [NBP1-84509] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human parathyroid gland shows very strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SAMM50-Antibody-Immunohistochemistry-Paraffin-NBP1-84509-img0024.jpg)
![Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human small intestine shows strong cytoplasmic granular positivity in glandular cells.](http://images.novusbio.com/fullsize/SAMM50-Antibody-Immunohistochemistry-Paraffin-NBP1-84509-img0019.jpg)
![Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human kidney shows very strong cytoplasmic granular positivity in cells in tubules.](http://images.novusbio.com/fullsize/SAMM50-Antibody-Immunohistochemistry-Paraffin-NBP1-84509-img0021.jpg)
![Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human pancreas shows moderate cytoplasmic granular positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/SAMM50-Antibody-Immunohistochemistry-Paraffin-NBP1-84509-img0022.jpg)
![Simple Western: SAMM50 Antibody [NBP1-84509] - Simple Western lane view shows a specific band for SAMM50 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SAMM50-Antibody-Simple-Western-NBP1-84509-img0008.jpg)
![Simple Western: SAMM50 Antibody [NBP1-84509] - Electropherogram image(s) of corresponding Simple Western lane view. SAMM50 antibody was used at 1:4 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/SAMM50-Antibody-Simple-Western-NBP1-84509-img0009.jpg)
![Western Blot: Adenylosuccinate Lyase Antibody [NBP1-87406] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ADSL antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Western-Blot-NBP1-87406-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Adenylosuccinate Lyase Antibody [NBP1-87406] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ADSL antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Adenylosuccinate Lyase Antibody [NBP1-87406] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87406-img0006.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody [NBP1-87406] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-87406-img0014.jpg)
![Western Blot: Adenylosuccinate Lyase Antibody [NBP1-87406] - Analysis in human cell line HL-60.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Western-Blot-NBP1-87406-img0005.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody [NBP1-87406] - Staining of human cerebral cortex shows no positivity in neuronal cells.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-87406-img0010.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody [NBP1-87406] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-87406-img0011.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody [NBP1-87406] - Staining of human lymphoid tissues shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-87406-img0012.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody [NBP1-87406] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-87406-img0013.jpg)
![Western Blot: PHF5A Antibody [NBP1-88591] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-PHF5A antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/PHF5A-Antibody-Western-Blot-NBP1-88591-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PHF5A Antibody [NBP1-88591] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-PHF5A antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PHF5A Antibody [NBP1-88591] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PHF5A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88591-img0009.jpg)
![Immunohistochemistry-Paraffin: PHF5A Antibody [NBP1-88591] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PHF5A-Antibody-Immunohistochemistry-Paraffin-NBP1-88591-img0014.jpg)
![Immunohistochemistry-Paraffin: PHF5A Antibody [NBP1-88591] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/PHF5A-Antibody-Immunohistochemistry-Paraffin-NBP1-88591-img0011.jpg)
![Immunohistochemistry-Paraffin: PHF5A Antibody [NBP1-88591] - Staining of human cerebral cortex shows strong nuclear positivity in neurons](http://images.novusbio.com/fullsize/PHF5A-Antibody-Immunohistochemistry-Paraffin-NBP1-88591-img0012.jpg)
![Immunohistochemistry-Paraffin: PHF5A Antibody [NBP1-88591] - Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PHF5A-Antibody-Immunohistochemistry-Paraffin-NBP1-88591-img0013.jpg)
