| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73034 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Non-Recombinant Monoclonal | NB600-1159SS | |||
| Non-Recombinant Monoclonal | NB600-1159 | |||
| BSA Free | NBP3-07012SS | |||
| BSA Free | NBP3-07012 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-18953SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22118SS | |||
| Azide and BSA Free | NBP2-80587 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-03955SS | |||
| Azide and BSA Free | NBP2-80917 | |||
![Western Blot: NONO Antibody (4D9) [NBP2-02060] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Western-Blot-NBP2-02060-img0017.jpg "Western Blot: NONO Antibody (4D9) [NBP2-02060] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: NONO Antibody (4D9) [NBP2-02060] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunocytochemistry-Immunofluorescence-NBP2-02060-img0016.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Human prostate tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0015.jpg)
![Flow Cytometry: NONO Antibody (4D9) [NBP2-02060] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NONO antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Flow-Cytometry-NBP2-02060-img0001.jpg)
![Western Blot: NONO Antibody (4D9) [NBP2-02060] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Western-Blot-NBP2-02060-img0002.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0003.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0004.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0006.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0007.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Human Ovary tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0014.jpg)
![Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Western-Blot-NBP2-37563-img0003.jpg "Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (5H7) [NBP2-37563] - Immunofluorescence analysis of NIH/3T3 cells using PHB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunocytochemistry-Immunofluorescence-NBP2-37563-img0004.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0013.jpg)
![Flow Cytometry: Prohibitin Antibody (5H7) [NBP2-37563] - Flow cytometric analysis of MCF-7 cells using PHB mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Flow-Cytometry-NBP2-37563-img0005.jpg)
![Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mAb against human PHB (AA: 68-259) recombinant protein. (Expected MW is 46.7 kDa)](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Western-Blot-NBP2-37563-img0002.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0010.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0011.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0012.jpg)
![ELISA: Prohibitin Antibody (5H7) [NBP2-37563] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-ELISA-NBP2-37563-img0001.jpg)
![Western Blot: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of THY1 on different lysates using anti-THY1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: HUVEC](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Western-Blot-NBP2-66763-img0007.jpg "Western Blot: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of THY1 on different lysates using anti-THY1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: HUVEC")
![Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Staining THY1 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunocytochemistry-Immunofluorescence-NBP2-66763-img0008.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded mouse hippocampus tissue using anti-CD90/Thy1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Staining THY1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunocytochemistry-Immunofluorescence-NBP2-66763-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Staining THY1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunocytochemistry-Immunofluorescence-NBP2-66763-img0002.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded human kidney tissue using anti-THY1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0003.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded human lung cancer tissue using anti-THY1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0004.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded human spleen tissue using anti-THY1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0005.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded human tonsil tissue using anti-THY1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0006.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded mouse brain tissue using anti-CD90/Thy1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0009.jpg)
![Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates with Rabbit anti-Lamin B1 antibody (10 ug/Lane Lane 2: PC-12 cell lysate, 10 ug/Lane Lane 3: Mouse liver tissue lysate, 20 ug/Lane Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Western-Blot-NBP2-67405-img0008.jpg "Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates with Rabbit anti-Lamin B1 antibody (10 ug/Lane Lane 2: PC-12 cell lysate, 10 ug/Lane Lane 3: Mouse liver tissue lysate, 20 ug/Lane Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of HepG2 cells labeling Lamin B1 with Rabbit anti-Lamin B1 antibody permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lamin B1 antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunocytochemistry-Immunofluorescence-NBP2-67405-img0009.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0012.jpg)
![Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysateLane 2: Jurkat cell lysate](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Western-Blot-NBP2-67405-img0006.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0001.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human colon cancer tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0002.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human liver tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded mouse colon tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0005.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Lamin B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0010.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0011.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 95 kDa Observed band size: 140/180 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0009.jpg "Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 95 kDa Observed band size: 140/180 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0007.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-NCAM-1/CD56 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0011.jpg)
![Flow Cytometry: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of SH-SY-5Y cells with NCAM antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Flow-Cytometry-NBP2-66968-img0001.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM on different lysates using anti-NCAM antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: Human brain](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0006.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on SH-SY5Y cell lysates with Rabbit anti-NCAM-1/CD56 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 95 kDa Observed band size: 120/140 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0003.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human tonsil tissue using anti-NCAM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0004.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded zebrafish kidney tissue using anti-NCAM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0005.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human tonsil tissue using anti-NCAM-1/CD56 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0010.jpg)
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)
![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Western Blot Analysis of human Prostate Cancer PC-3 cell lysate Cytokeratin 19 Antibody (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Western-Blot-NBP2-33134-img0007.jpg "Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Western Blot Analysis of human Prostate Cancer PC-3 cell lysate Cytokeratin 19 Antibody (A53-B/A2.26).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Immunofluorescence Analysis of MCF-7 cells labeling Cytokeratin 19 with Cytokeratin 19 Antibody (A53-B/A2.26) followed by Goat anti-Mouse IgG-CF488 (Cyan). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33134-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Formalin-paraffin colon carcinoma stained with Cytokeratin 19 Ab (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33134-img0008.jpg)
![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Western blot 1) Hep-G2 and 2) MCF-7 lysate using of Cytokeratin 19 Antibody (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Western-Blot-NBP2-33134-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Cytokeratin 19 (A53-B/A2.26) conjugated to Alexa Fluor 488 [NBP2-33134AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33134-img0004.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Breast cancer tissue stained for Cytokeratin 19 (red) counter stained with DAPI (blue). The Janelia-549 conjugate of this antibody was used. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunohistochemistry-Frozen-NBP2-33134-img0001.jpg)
![Simple Western: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Simple Western lane view shows a specific band for Cytokeratin 19 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Simple-Western-NBP2-33134-img0002.jpg)
![Simple Western: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 19 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Simple-Western-NBP2-33134-img0003.jpg)
![Western Blot: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Detection by Western blot in RL-7 cells (a follicular lymphoma).](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Western-Blot-NBP2-33250-img0004.jpg "Western Blot: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Detection by Western blot in RL-7 cells (a follicular lymphoma).")
![Immunocytochemistry/Immunofluorescence: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - ICC/IF analysis in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:10 was used. Image objective 40x.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-33250-img0002.jpg)
![Immunohistochemistry-Paraffin: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Immunohistochemical analysis of bouin-fixed, paraffin-embedded chronic lymphocytic leukemia (CLL) xenograft stained with Noxa antibody (1:2000), peroxidase-conjugate and DAB chromogen. Staining for 2 hr at RT was used.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Immunohistochemistry-Paraffin-NBP2-33250-img0003.jpg)
![Flow Cytometry: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Flow cytometry after an intracellular stain was performed on HeLa cells with Noxa [114C307.1] antibody NB600-1159AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Flow-Cytometry-NBP2-33250-img0007.jpg)
![Flow Cytometry: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Flow cytometry after an intracellular stain was performed on MCF7 cells with Noxa [114C307.1] antibody NB600-1159AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Flow-Cytometry-NBP2-33250-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HPd1 Antibody (DHIC2-4A10) [NBP1-18953] - Immunofluorescence on frozen section of human pancreas.](http://images.novusbio.com/fullsize/HPd1-Antibody-DHIC2-4A10-Immunocytochemistry-Immunofluorescence-NBP1-18953-img0005.jpg "Immunocytochemistry/Immunofluorescence: HPd1 Antibody (DHIC2-4A10) [NBP1-18953] - Immunofluorescence on frozen section of human pancreas.")
![Flow Cytometry: HPd1 Antibody (DHIC2-4A10) [NBP1-18953] - Analysis of enzyme dispersed human pancreas cells.](http://images.novusbio.com/fullsize/HPd1-Antibody-DHIC2-4A10-Flow-Cytometry-NBP1-18953-img0004.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: Histone H3 antibody diluted at 1:500. Red: alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0018.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: Histone H3 antibody diluted at 1:500. Red: alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500.")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0002.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Paraffin-embedded mouse colon. Histone H3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0003.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Paraffin-embedded mouse brain. Histone H3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0004.jpg)
![Immunoprecipitation: HIST1H3A Antibody [NBP2-16842] - IP Sample: Raji whole cell lysate/extract A : Control with 3 ug of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 ug of Histone H3 antibody15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody (diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunoprecipitation-NBP2-16842-img0009.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16842] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HIST1H3A antibody (NBP2-16842) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16842_rabbit-polyclonal-hist1h3a-antibody-9102024202460.jpg)
![Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Western-Blot-NBP2-22118-img0002.jpg "Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.")
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of Hela cells using Aurora A mouse mAb (green). DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Immunocytochemistry-Immunofluorescence-NBP2-22118-img0004.jpg)
![Flow Cytometry: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of K562 cells using Aurora A mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Flow-Cytometry-NBP2-22118-img0003.jpg)
![ELISA: Aurora A Antibody (1F8) [NBP2-22118] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-ELISA-NBP2-22118-img0001.jpg)
![Western Blot: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Azide Free version of NBP2-03955. Lysate from mouse A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) Stomach, I) Spleen, J) Ovary, and K) Testis probed with podoplanin antibody at 5 ug/mL. Mouse tissue Insta-blot, goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-pmab-1-Western-Blot-NBP2-03955-img0013.jpg "Western Blot: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Azide Free version of NBP2-03955. Lysate from mouse A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) Stomach, I) Spleen, J) Ovary, and K) Testis probed with podoplanin antibody at 5 ug/mL. Mouse tissue Insta-blot, goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the PE conjugate of NBP2-03955. Staining using podoplanin antibody. Mouse F9 tumor cell line probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green). Cell surface flow kit was used for this test.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-pmab-1-Flow-Cytometry-NBP2-03955-img0010.jpg)
![Western Blot: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Azide Free version of NBP2-03955. Detection of mouse fetal lung tissue using Podoplanin antibody at a conentration of 1 ug/mL.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-pmab-1-Western-Blot-NBP2-03955-img0012.jpg)
![Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using podoplanin antibody. Mouse LLC1 cells were probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green). NBP2-03955 and APC-conjugated secondary antibody were used for this test.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-pmab-1-Flow-Cytometry-NBP2-03955-img0004.jpg)
![Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-03955. Staining using podoplanin antibody. Mouse LLC1 cells were probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green).](http://images.novusbio.com/fullsize/Podoplanin-Antibody-pmab-1-Flow-Cytometry-NBP2-03955-img0008.jpg)
![Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-03955. Staining using podoplanin antibody. Mouse LLC1 cells were probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green).](http://images.novusbio.com/fullsize/Podoplanin-Antibody-pmab-1-Flow-Cytometry-NBP2-03955-img0009.jpg)