| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72846 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73271 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65900-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72941 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77030-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71591 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73822 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70314 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74840 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72787 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77179-0.025mg | |||
![Western Blot: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Myeloid leukemia factor 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Myeloid leukemia factor 1.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Western-Blot-NBP2-02052-img0001.jpg "Western Blot: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Myeloid leukemia factor 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Myeloid leukemia factor 1.")
![Immunocytochemistry/Immunofluorescence: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Myeloid leukemia factor 1.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Immunocytochemistry-Immunofluorescence-NBP2-02052-img0009.jpg)
![Immunohistochemistry-Paraffin: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - Staining of paraffin-embedded Human tonsil using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Immunohistochemistry-Paraffin-NBP2-02052-img0008.jpg)
![Flow Cytometry: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - HEK293T cells transfected with either Myeloid leukemia factor 1 Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MLF1 antibody , and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Flow-Cytometry-NBP2-02052-img0010.jpg)
![Immunohistochemistry-Paraffin: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Immunohistochemistry-Paraffin-NBP2-02052-img0002.jpg)
![Immunohistochemistry-Paraffin: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Immunohistochemistry-Paraffin-NBP2-02052-img0003.jpg)
![Immunohistochemistry-Paraffin: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Immunohistochemistry-Paraffin-NBP2-02052-img0004.jpg)
![Immunohistochemistry-Paraffin: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Immunohistochemistry-Paraffin-NBP2-02052-img0005.jpg)
![Immunohistochemistry-Paraffin: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Immunohistochemistry-Paraffin-NBP2-02052-img0006.jpg)
![Immunohistochemistry-Paraffin: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - Staining of paraffin-embedded Human pancreas tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Immunohistochemistry-Paraffin-NBP2-02052-img0007.jpg)
![Western Blot: PCNA Antibody (3D6) [NBP2-46342] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA.](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Western-Blot-NBP2-46342-img0022.jpg "Western Blot: PCNA Antibody (3D6) [NBP2-46342] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA.")
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0021.jpg)
![Western Blot: PCNA Antibody (3D6) [NBP2-46342] - Analysis of extracts (35ug) from 9 different cell lines by using PCNA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:2000](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Western-Blot-NBP2-46342-img0023.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0001.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0002.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0003.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0004.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0005.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0006.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0007.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0008.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0009.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0010.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0011.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0012.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0013.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0014.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0015.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0016.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0017.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0018.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0019.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0020.jpg)
![Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Western-Blot-NB100-74502-img0004.jpg "Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.")
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in WiDr colon carcinoma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0010.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (13E8) [NB100-74502] - Both normal and cancer biopsies of deparaffinized human Colon tissue.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunohistochemistry-Paraffin-NB100-74502-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in U251 glioma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0009.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Valvular endothelial cell (VEC) markers. Sections of the sheep aortic valve leaflet stained with antibodies against the endothelial cell markers CD31/PECAM-1, CD34, VE-Cadherin (CD144), or von Willebrand Factor (vWF). Represented are the aortic wall and a section of the valvular leaflet (near the free edge), with](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-CO-3E-1D4-Immunohistochemistry-Paraffin-NB100-65900-img0007.jpg "Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Valvular endothelial cell (VEC) markers. Sections of the sheep aortic valve leaflet stained with antibodies against the endothelial cell markers CD31/PECAM-1, CD34, VE-Cadherin (CD144), or von Willebrand Factor (vWF). Represented are the aortic wall and a section of the valvular leaflet (near the free edge), with")
![Flow Cytometry: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Staining of sheep peripheral blood platelets with Mouse anti Bovine CD31/PECAM-1 Antibody (CO.3E-1D4).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-CO-3E-1D4-Flow-Cytometry-NB100-65900-img0005.jpg)
![Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Bovine Lung stained with CD31 (green) and DAPI counterstain. Image provided courtesy of Hala Nijmeh, PhD and the PI, Evgenia V. Gerasimovskaya, PhD. Section of Critical Care Medicine, Department of Pediatrics, University of Colorado Anschutz Medical Center.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-CO-3E-1D4-Immunohistochemistry-Frozen-NB100-65900-img0006.jpg)
![Western Blot: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Neprilysin/CD10.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Western-Blot-NBP2-45817-img0010.jpg "Western Blot: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Neprilysin/CD10.")
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Carcinoma of Human bladder tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0009.jpg)
![Flow Cytometry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - analysis of living 293T cells transfected with MME overexpression plasmid .](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Flow-Cytometry-NBP2-45817-img0011.jpg)
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0001.jpg)
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0002.jpg)
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0003.jpg)
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0004.jpg)
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0005.jpg)
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0006.jpg)
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0007.jpg)
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0008.jpg)
![Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 15 ug of lysates per lane.Antibodies: SCF (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/SCF-c-kit-Ligand-Antibody-Western-Blot-NBP1-77030-img0012.jpg "Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 15 ug of lysates per lane.Antibodies: SCF (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Analysis of SCF in A431 cell lysate with SCF antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/SCF-c-kit-Ligand-Antibody-Western-Blot-NBP1-77030-img0006.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 30 ng of human SCF recombinant protein per lane.Antibodies: SCF (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 20kD.](http://images.novusbio.com/fullsize/SCF-c-kit-Ligand-Antibody-Western-Blot-NBP1-77030-img0011.jpg)
![Immunohistochemistry: SCF/c-kit Ligand Antibody - BSA Free [NBP1-77030] - Immunohistochemistry Validation of SCF/c-kit Ligand in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SCF/c-kit Ligand antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77030_rabbit-polyclonal-scf-c-kit-ligand-antibody-2711202420441113.jpg)
![Immunocytochemistry/ Immunofluorescence: SCF/c-kit Ligand Antibody - BSA Free [NBP1-77030] - Immunofluorescence Validation of SCF/c-kit Ligand in Human Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling SCF/c-kit Ligand with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77030_rabbit-polyclonal-scf-c-kit-ligand-antibody-271120241928812.jpg)
![Western Blot: AKR1A1 Antibody (6E3) [NBP2-02164] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-6E3-Western-Blot-NBP2-02164-img0004.jpg "Western Blot: AKR1A1 Antibody (6E3) [NBP2-02164] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.")
![Immunocytochemistry/Immunofluorescence: AKR1A1 Antibody (6E3) [NBP2-02164] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKR1A1.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-6E3-Immunocytochemistry-Immunofluorescence-NBP2-02164-img0007.jpg)
![Immunohistochemistry-Paraffin: AKR1A1 Antibody (6E3) [NBP2-02164] - Staining of paraffin-embedded liver tissue using anti-AKR1A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-6E3-Immunohistochemistry-Paraffin-NBP2-02164-img0006.jpg)
![Flow Cytometry: AKR1A1 Antibody (6E3) [NBP2-02164] - HEK293T cells transfected with either pCMV6-ENTRY AKR1A1.(Red) or empty vector control plasmid (Blue) were immunostained with anti-AKR1A1 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-6E3-Flow-Cytometry-NBP2-02164-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: AKR1A1 Antibody (6E3) [NBP2-02164] - Immunofluorescent staining of HepG2 cells using anti-AKR1A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-6E3-Immunocytochemistry-Immunofluorescence-NBP2-02164-img0005.jpg)
![Flow Cytometry: AKR1A1 Antibody (6E3) [NBP2-02164] - Analysis of Hela cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/AKR1A1-Antibody-6E3-Flow-Cytometry-NBP2-02164-img0001.jpg)
![Flow Cytometry: AKR1A1 Antibody (6E3) [NBP2-02164] - Analysis of Jurkat cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/AKR1A1-Antibody-6E3-Flow-Cytometry-NBP2-02164-img0002.jpg)
![Western Blot: RbAp46 Antibody (5A4) [NBP2-01308] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RbAp46 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RbAp46.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Western-Blot-NBP2-01308-img0001.jpg "Western Blot: RbAp46 Antibody (5A4) [NBP2-01308] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RbAp46 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RbAp46.")
![Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] Staining of paraffin-embedded Human prostate tissue using anti-RbAp46 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Immunohistochemistry-Paraffin-NBP2-01308-img0009.jpg)
![Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-RbAp46 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Immunohistochemistry-Paraffin-NBP2-01308-img0002.jpg)
![Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RbAp46 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Immunohistochemistry-Paraffin-NBP2-01308-img0003.jpg)
![Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RbAp46 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Immunohistochemistry-Paraffin-NBP2-01308-img0004.jpg)
![Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RbAp46 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Immunohistochemistry-Paraffin-NBP2-01308-img0005.jpg)
![Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Human endometrium tissue using anti-RbAp46 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Immunohistochemistry-Paraffin-NBP2-01308-img0006.jpg)
![Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Human Kidney tissue using anti-RbAp46 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Immunohistochemistry-Paraffin-NBP2-01308-img0007.jpg)
![Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Human pancreas tissue using anti-RbAp46 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Immunohistochemistry-Paraffin-NBP2-01308-img0008.jpg)
![Western Blot: BubR1 Antibody (6E5) [NBP1-47680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Western-Blot-NBP1-47680-img0002.jpg "Western Blot: BubR1 Antibody (6E5) [NBP1-47680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.")
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (OTI6E5) [NBP1-47680] - BubR1 Antibody (6E5) [NBP1-47680] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BubR1.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunocytochemistry-Immunofluorescence-NBP1-47680-img0012.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded prostate tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0009.jpg)
![Flow Cytometry: BubR1 Antibody (6E5) [NBP1-47680] - HEK293T cells transfected with either pCMV6-ENTRY BubR1.(Red) or empty vector control plasmid (Blue) were immunostained with anti-BubR1 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Flow-Cytometry-NBP1-47680-img0011.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0003.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0004.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0005.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded colon tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0006.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded endometrium tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0007.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded pancreas tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0008.jpg)
![Western Blot: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Analysis of extracts (35ug) from 9 different cell lines by using anti-VCAM1 monoclonal antibody.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Western-Blot-NBP2-03600-img0021.jpg "Western Blot: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Analysis of extracts (35ug) from 9 different cell lines by using anti-VCAM1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VCAM1.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Immunocytochemistry-Immunofluorescence-NBP2-03600-img0015.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Human prostate tissue using anti-VCAM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Immunohistochemistry-Paraffin-NBP2-03600-img0019.jpg)
![Western Blot: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Western-Blot-NBP2-03600-img0020.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VCAM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Immunohistochemistry-Paraffin-NBP2-03600-img0016.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - CD106 / VCAM1 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-VCAM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Immunohistochemistry-Paraffin-NBP2-03600-img0017.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Human Kidney tissue using anti-VCAM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Immunohistochemistry-Paraffin-NBP2-03600-img0018.jpg)
![Western Blot: MRPS27 Antibody (OTI6E5) [NBP2-45938] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27.](http://images.novusbio.com/fullsize/MRPS27-Antibody-OTI6E5-Western-Blot-NBP2-45938-img0015.jpg "Western Blot: MRPS27 Antibody (OTI6E5) [NBP2-45938] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27.")
![Immunohistochemistry: MRPS27 Antibody (OTI6E5) [NBP2-45938] - Analysis of Human colon tissue.](http://images.novusbio.com/fullsize/MRPS27-Antibody-OTI6E5-Immunohistochemistry-NBP2-45938-img0014.jpg)
![Flow Cytometry: MRPS27 Antibody (OTI6E5) [NBP2-45938] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by MRPS27 antibody.](http://images.novusbio.com/fullsize/MRPS27-Antibody-OTI6E5-Flow-Cytometry-NBP2-45938-img0001.jpg)
![Immunohistochemistry: MRPS27 Antibody (OTI6E5) [NBP2-45938] - Analysis of Adenocarcinoma of Human colon tissue.](http://images.novusbio.com/fullsize/MRPS27-Antibody-OTI6E5-Immunohistochemistry-NBP2-45938-img0003.jpg)
![Immunohistochemistry: MRPS27 Antibody (OTI6E5) [NBP2-45938] - Analysis of Carcinoma of Human lung tissue.](http://images.novusbio.com/fullsize/MRPS27-Antibody-OTI6E5-Immunohistochemistry-NBP2-45938-img0004.jpg)
![Immunohistochemistry: MRPS27 Antibody (OTI6E5) [NBP2-45938] - Analysis of Carcinoma of Human prostate tissue.](http://images.novusbio.com/fullsize/MRPS27-Antibody-OTI6E5-Immunohistochemistry-NBP2-45938-img0005.jpg)
![Immunohistochemistry: MRPS27 Antibody (OTI6E5) [NBP2-45938] - Analysis of Human lung tissue.](http://images.novusbio.com/fullsize/MRPS27-Antibody-OTI6E5-Immunohistochemistry-NBP2-45938-img0006.jpg)
![Immunohistochemistry: MRPS27 Antibody (OTI6E5) [NBP2-45938] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/MRPS27-Antibody-OTI6E5-Immunohistochemistry-NBP2-45938-img0007.jpg)
![Immunohistochemistry: MRPS27 Antibody (OTI6E5) [NBP2-45938] - Analysis of Carcinoma of Human liver tissue.](http://images.novusbio.com/fullsize/MRPS27-Antibody-OTI6E5-Immunohistochemistry-NBP2-45938-img0008.jpg)
![Immunohistochemistry: MRPS27 Antibody (OTI6E5) [NBP2-45938] - Analysis of Adenocarcinoma of Human breast tissue.](http://images.novusbio.com/fullsize/MRPS27-Antibody-OTI6E5-Immunohistochemistry-NBP2-45938-img0009.jpg)
![Immunohistochemistry: MRPS27 Antibody (OTI6E5) [NBP2-45938] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/MRPS27-Antibody-OTI6E5-Immunohistochemistry-NBP2-45938-img0010.jpg)
![Immunohistochemistry: MRPS27 Antibody (OTI6E5) [NBP2-45938] - Analysis of Carcinoma of Human pancreas tissue.](http://images.novusbio.com/fullsize/MRPS27-Antibody-OTI6E5-Immunohistochemistry-NBP2-45938-img0011.jpg)
![Immunohistochemistry: MRPS27 Antibody (OTI6E5) [NBP2-45938] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/MRPS27-Antibody-OTI6E5-Immunohistochemistry-NBP2-45938-img0012.jpg)
![Immunohistochemistry: MRPS27 Antibody (OTI6E5) [NBP2-45938] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/MRPS27-Antibody-OTI6E5-Immunohistochemistry-NBP2-45938-img0013.jpg)
![Immunofluorescence: MRPS27 Antibody (OTI6E5) [NBP2-45938] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MRPS27.](http://images.novusbio.com/fullsize/MRPS27-Antibody-OTI6E5-Immunofluorescence-NBP2-45938-img0002.jpg)
![Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-77179] - Analysis in EL4 cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 ug/ml.Red: ZEB2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202419333477.gif)
![Western Blot: ZEB2 Antibody [NBP1-77179] - Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, and then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0012.jpg)
![Western Blot: ZEB2 Antibody [NBP1-77179] - The protein levels of EMT-TFs were compared between carcinoma cell lines and non- or less-malignant tumor cell lines by using Western-blot analysis. The soft agar colony transformation or migration assay was referred to distinguish malignant degree of the experimental cancer cell lines (data not shown). Malignant degree: prostate cancer cell lines, P69 < M12; breast cancer cell lines, MCF7 < MD-MBA-231; lung cancer cell lines, A549 < H1299; liver cancer cell lines, HepG2 < SMMC7721. 'kD' (kilo-dalton) means the molecular weight of protein. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0013.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415392525.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415334920.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415395947.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-31020241535644.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Functional domains of Fbxo45 for ubiquitination of Zeb2A. Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, & then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. B. Bacterially expressed GST or GST–Fbxo45, -Fbxo45 delta F-box, -Fbxo45 delta mid, or -Fbxo45 delta SPRY fusion proteins & Glutathione-Sepharose beads were incubated with lysates of HeLa cells transfected with Zeb2, Snai1 or Snai2, respectively. The proteins associated with GST–tagged Fbxo45 forms, bound on the Glutathione-Sepharose beads were washed five times with the RIPA buffer before immunoblotting. C. Lysates from 293T cells transfected Flag-Zeb2 with HA-Fbxo45, -Fbxo45 delta mid, -Fbxo45 delta F-box or -Fbxo45 delta SPRY were immunoprecipitated with anti-Flag or anti-HA antibodies, & immunoprecipitates were resolved by SDS-PAGE for Western-blot analysis. D. U2OS cells transfected with Flag-Zeb2 & HA-Fbxo45, -Fbxo45 delta F-box or -Fbxo45 delta SPRY were stained using the primary antibodies of anti-Flag M2 or anti-HA, & the second antibodies of Alexa Fluor 568 anti-mouse or Alexa Fluor 488 anti-Rabbit, respectively. Scale: 25μm. E-I. Zeb2 ubiquitination assays by using IP expriments under different conditions: Flag-Zeb2 with or without HA-Ub (E); Flag-Zeb2 with HA-Ub WT, K48R or K63R (F); Flag-Zeb2 & HA-Ub K48-only with or without myc-Fbox45 (G); Flag-Zeb2 & HA-Ub K48-only with siRNA control, siRNAs for Fbxo45 or siRNAs for Pam (H); Flag-Zeb2 & HA-Ub with HA-Fbxo45, -Fbxo45 delta F-box or -Fbxo45 delta SPRY (I). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202416163752.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 is a direct target of miR-27a* that mediates EMT processesA, C. The expression levels of miR-27a* (A) & Fbxo45 mRNA (C) in P69 & M12 were shown according to the data of miRNA transcriptome sequencing & DGE sequencing, respectively [46]. TPM means transcripts per million. B. Real-time PCR in delta delta Ct methods were used to confirm the expression levels of miR-27a* in P69 & M12. One representative experiment out of three was shown. D. Fbxo45 mRNA levels in stable cell lines miR-27a*-overexpressing P69 & miR-27a*-silencing M12 (zipmiR-27a*). P69 or M12 stably infected with the same lentiviral empty vector was used as a control (ctrl). E. Fbxo45 3′-UTR wide-type or mutant form at the position where is complementary to the 5′ seed region of miR-27a* was subcloned into psiCheck2 vector. 293T cells were transfected with psiCheck2 containing WT or mutant Fbxo45 3′-UTR & miR-27a* or non-specific control vector. The Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. Data are the mean±S.E.M. of three independent experiments. F-G. Immunoblot for Fbxo45, core EMT-TFs (Zeb1, Zeb2, Twist1) & their associated EMT markers (Fibronectin, N-cadherin, Laminin C2, Vimentin & E-cadherin) in stable cell lines P69-miR-27a* or M12-zipmiR-27a* (F); Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20μM of MG132 for 4 h before harvested (G). GAPDH is for a loading control. H. Immunblot for Fbxo45, N-cadherin, E-cadherin & five core EMT-TFs in stable cell lines PC3-ctrl, PC3-miR-27a* & PC3-zipmiR-27a*. alpha -tubulin is for a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202416175234.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 is a direct target of miR-27a* that mediates EMT processesA, C. The expression levels of miR-27a* (A) & Fbxo45 mRNA (C) in P69 & M12 were shown according to the data of miRNA transcriptome sequencing & DGE sequencing, respectively [46]. TPM means transcripts per million. B. Real-time PCR in delta delta Ct methods were used to confirm the expression levels of miR-27a* in P69 & M12. One representative experiment out of three was shown. D. Fbxo45 mRNA levels in stable cell lines miR-27a*-overexpressing P69 & miR-27a*-silencing M12 (zipmiR-27a*). P69 or M12 stably infected with the same lentiviral empty vector was used as a control (ctrl). E. Fbxo45 3′-UTR wide-type or mutant form at the position where is complementary to the 5′ seed region of miR-27a* was subcloned into psiCheck2 vector. 293T cells were transfected with psiCheck2 containing WT or mutant Fbxo45 3′-UTR & miR-27a* or non-specific control vector. The Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. Data are the mean±S.E.M. of three independent experiments. F-G. Immunoblot for Fbxo45, core EMT-TFs (Zeb1, Zeb2, Twist1) & their associated EMT markers (Fibronectin, N-cadherin, Laminin C2, Vimentin & E-cadherin) in stable cell lines P69-miR-27a* or M12-zipmiR-27a* (F); Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20μM of MG132 for 4 h before harvested (G). GAPDH is for a loading control. H. Immunblot for Fbxo45, N-cadherin, E-cadherin & five core EMT-TFs in stable cell lines PC3-ctrl, PC3-miR-27a* & PC3-zipmiR-27a*. alpha -tubulin is for a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-31020241616011.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunocytochemistry of ZEB2 in Jurkat cells with ZEB2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202420285893.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202420272496.jpg)