| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34685-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48154 | |||
| Azide and BSA Free | NBP2-34634-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1598SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33060 | |||
| IHC-Prediluted | NBP2-44830 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24941SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54392-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47916-0.1mg | |||
| IHC-Prediluted | NBP2-48305 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27403 | |||
| BSA Free | NB100-56546SS | |||
![Western Blot: Moesin Antibody (MSN/491) [NBP2-32875] - Western Blot Analysis of human PC3 cell lysate using Moesin Antibody (MSN/491).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Western-Blot-NBP2-32875-img0014.jpg "Western Blot: Moesin Antibody (MSN/491) [NBP2-32875] - Western Blot Analysis of human PC3 cell lysate using Moesin Antibody (MSN/491).")
![Immunocytochemistry/Immunofluorescence: Moesin Antibody (MSN/491) [NBP2-32875] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Moesin with Moesin Antibody (MSN/491) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Immunocytochemistry-Immunofluorescence-NBP2-32875-img0013.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) [NBP2-32875] - Fomalin-paraffin Rat colon stained with Moesin MAb (MSN/491)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Immunohistochemistry-Paraffin-NBP2-32875-img0010.jpg)
![Flow Cytometry: Moesin Antibody (MSN/491) [NBP2-32875] - Flow Cytometric Analysis of K562 cells using Moesin Antibody (MSN/491) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Flow-Cytometry-NBP2-32875-img0012.jpg)
![Western Blot: Moesin Antibody (MSN/491) [NBP2-32875] - Analysis using the Azide and BSA Free version of NBP2-32875. Detection of Moesin in human HT29 Cells.](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Western-Blot-NBP2-32875-img0006.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) [NBP2-32875] - Formalin-paraffin human placenta stained with Moesin MAb (MSN/491)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Immunohistochemistry-Paraffin-NBP2-32875-img0007.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) [NBP2-32875] - Formalin-paraffin human Melanoma stained with Moesin Mab (MSN/491)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Immunohistochemistry-Paraffin-NBP2-32875-img0008.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) [NBP2-32875] - Formalin-paraffin Rat Lung stained with Moesin MAb (MSN/491)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Immunohistochemistry-Paraffin-NBP2-32875-img0009.jpg)
![Simple Western: Moesin Antibody (MSN/491) [NBP2-32875] - Simple Western lane view shows a specific band for Moesin in 0.1 mg/ml of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Simple-Western-NBP2-32875-img0003.jpg)
![Simple Western: Moesin Antibody (MSN/491) [NBP2-32875] - Electropherogram image of the corresponding Simple western lane view. Moesin antibody was used at 1 ug/ml dilution on HUVEC lysate(s) respectively.](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Simple-Western-NBP2-32875-img0004.jpg)
![Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Endometrium muscle tissue lysate using pan Actin Antibody (HHF35).](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Western-Blot-NBP2-34230-img0011.jpg "Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Endometrium muscle tissue lysate using pan Actin Antibody (HHF35).")
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Formalin-fixed paraffin-embedded human Rhabdomyosarcoma stained with muscle specific actin monoclonal antibody (HHF35)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Immunohistochemistry-Paraffin-NBP2-34230-img0008.jpg)
![Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Skeletal muscle tissue lysate using pan Actin Antibody (HHF35).](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Western-Blot-NBP2-34230-img0010.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Formalin-fixed, paraffin-embedded human leiomyosarcoma (10X) stained with muscle specific actin MAb (HHF35)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Immunohistochemistry-Paraffin-NBP2-34230-img0004.jpg)
![Simple Western: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Simple Western lane view shows a specific band for pan Actin in 0.2 mg/ml of Muscle (Sk) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Simple-Western-NBP2-34230-img0005.jpg)
![Simple Western: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Electropherogram image of the corresponding Simple Western lane. pan Actin antibody was used at 10 ug/ml dilution of Muscle (Sk) lysates(s) respectively.](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Simple-Western-NBP2-34230-img0007.jpg)
![Western Blot: eIF4A1 Antibody [NBP2-24632] - Representative immunoblot of total lysate of MEFs with eIF4A (C-terminal epitope) and Tubulin antibodies in control and GS (glucose starvation). Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/21907) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/eIF4A1-Antibody-Western-Blot-NBP2-24632-img0003.jpg "Western Blot: eIF4A1 Antibody [NBP2-24632] - Representative immunoblot of total lysate of MEFs with eIF4A (C-terminal epitope) and Tubulin antibodies in control and GS (glucose starvation). Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/21907) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: eIF4A1 Antibody [NBP2-24632] - Analysis of eIF4A1 in human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/eIF4A1-Antibody-Immunohistochemistry-Paraffin-NBP2-24632-img0001.jpg)
![Western Blot: eIF4A1 Antibody [NBP2-24632] - Analysis of eIF4A1 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) RAW cell lysate in the absence of immunizing peptide using NBP2-24632 at 0.25 ug/ml.](http://images.novusbio.com/fullsize/eIF4A1-Antibody-Western-Blot-NBP2-24632-img0002.jpg)
![Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Western-Blot-NB100-1598-img0013.jpg "Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CRIPTO Antibody [NB100-1598] - CRIPTO antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1598-img0010.jpg)
![Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on Jurkat with NB100-1598 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Flow-Cytometry-NB100-1598-img0012.jpg)
![Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on HeLa cells with NB100-1598PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Flow-Cytometry-NB100-1598-img0011.jpg)
![Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Knockout-Validated-NBP2-29433-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0008.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM452) [NBP2-29433] - Formalin-paraffin human melanoma stained with Vimentin Ab (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Immunohistochemistry-Paraffin-NBP2-29433-img0002.jpg)
![Flow Cytometry: Vimentin Antibody (VM452) [NBP2-29433] - Flow Cytometry of human Vimentin on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Flow-Cytometry-NBP2-29433-img0004.jpg)
![Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis Raji cell lysate Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0007.jpg)
![Protein Array: Vimentin Antibody (VM452) [NBP2-29433] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin Antibody (VM452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Protein-Array-NBP2-29433-img0006.jpg)
![Western Blot: Nanog Antibody [NBP2-24941] - Analysis of Nanog in (A) HeLa and (B) NIH 3T3 cell lysate using NBP2-24941 at 2 ug/ml.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NBP2-24941-img0002.jpg "Western Blot: Nanog Antibody [NBP2-24941] - Analysis of Nanog in (A) HeLa and (B) NIH 3T3 cell lysate using NBP2-24941 at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Nanog Antibody [NBP2-24941] - Nanog antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40x. An antibody dilution of 1:10 was used.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24941-img0005.jpg)
![Western Blot: Nanog Antibody [NBP2-24941] - Protein expression of pluripotency markers in uterine cells isolated from control cows and from cows with adenomyosis. MM - molecular weight marker, C - cells obtained from control cows, ADENO - cells obtained from cows with adenomyosis. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/s12958-015-0106-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NBP2-24941-img0006.jpg)
![Immunohistochemistry: Nanog Antibody [NBP2-24941] - Immunodetection of pluripotency markers in uterine tissues from control cows & from cows with adenomyosis. a, b – haematoxylin & eosin stained slides of control & adenomyotic uterus, respectively; c, d – NANOG immunodetection in control & adenomyotic tissue, respectively; e, f – immunolocalisation of OCT4 in normal & adenomyotic tissue, respectively; g, h – SOX2 immunodetection in control & adenomyotic tissue, respectively; i, j, k – no Ab, negative controls for NANOG, OCT4 & SOX2, respectively. Unspecific IgG controls (pictures not shown) served similar pictures as no Ab control. Arrows indicate the most intense histochemical reactions; dotted line indicate endometrial-myometrial border; e – endometrium, m – myometrium, es – endometrial stroma, eg – endometrial gland, a – adenomyotic lesion, v – vessel. Scale bars: 20 μm Image collected & cropped by CiteAb from the following publication (//www.rbej.com/content/13/1/110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-24941_rabbit-polyclonal-nanog-antibody-310202416121911.jpg)
![Western Blot: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1308).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1308-Western-Blot-NBP2-53387-img0005.jpg)
![Flow Cytometry: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Flow Cytometric Analysis of Jurkat cells using CD3 epsilon Antibody (C3e/1308) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1308-Flow-Cytometry-NBP2-53387-img0004.jpg)
![Western Blot: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Western Blot of recombinant CD3e and Jurkat Cell Lysate using CD3e Monoclonal Antibody (C3e/1308)](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1308-Western-Blot-NBP2-53387-img0002.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Staining of T cells in human FFPE tonsil tissue using AF647-conjugated CD3 epsilon Antibody (Catalog # NBP2-54392AF647). Antigen Retrieval at pH 9. Antibody incubation over night at 4C. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-53387_mouse-monoclonal-cd3-epsilon-antibody-c3e-1308-17720241003230.jpg)
![Western Blot: Moesin Antibody (MSN/492) [NBP2-44579] - Western blot using lysates from U20S parental cell line and Moesin-1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Moesin Monoclonal Antibody (Catalog # NBP2-44579) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Moesin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Knockout-Validated-NBP2-44579-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Moesin Antibody (MSN/492) [NBP2-44579] - Western blot using lysates from U20S parental cell line and Moesin-1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Moesin Monoclonal Antibody (Catalog # NBP2-44579) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Moesin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Moesin Antibody (MSN/492) [NBP2-44579] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells. followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Immunocytochemistry-Immunofluorescence-NBP2-44579-img0005.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579] - Human Melanoma stained with Moesin Monoclonal Antibody (MSN/492)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Immunohistochemistry-Paraffin-NBP2-44579-img0001.jpg)
![Western Blot: Moesin Antibody (MSN/492) [NBP2-44579] - Western Blot Analysis of PC-3 cell lysate using Moesin antibody (MSN/492).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Western-Blot-NBP2-44579-img0009.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579] - Human Testicular Carcinoma stained with Moesin Monoclonal Antibody (MSN/492)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Immunohistochemistry-Paraffin-NBP2-44579-img0003.jpg)
![Flow Cytometry: Moesin Antibody (MSN/492) [NBP2-44579] - Flow Cytometric Analysis of K562 cells. Moesin Antibody (MSN/492) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Flow-Cytometry-NBP2-44579-img0004.jpg)
![Western Blot: Moesin Antibody (MSN/492) [NBP2-44579] - Western Blot Analysis of human Jurkat cell lysate using Moesin Antibody (MSN/492).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Western-Blot-NBP2-44579-img0006.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579] - Human Placenta stained with Moesin Monoclonal Antibody (MSN/492)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Immunohistochemistry-Paraffin-NBP2-44579-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Moesin Antibody (MSN/492) [NBP2-44579] - Parental and MSN KO cells were labeled with a green or a far-red dye, respectively. Parental and KO cells were mixed and plated to a 1:1 ratio on coverslips. Cells were stained with anti-Moesin (NBP2-44579) antibodies and with the corresponding Alexa-fluor 555 coupled secondary antibody. Acquisition of the green (WT), red (antibody staining), and far-red (KO) channels was performed. Representative grayscale images of the red channel are shown, and WT and KO cells are outlined with a yellow and magenta dashed line, respectively. A schematic representation of the mosaic strategy used is shown on the right panel. Antibody dilution used: NBP2-44579 at 1/200. Bar = 10 um. Images and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Knockout-Validated-NBP2-44579-img0008.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml. Image using the Azide Free form of this antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Western-Blot-NB100-56546-img0003.jpg "Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml. Image using the Azide Free form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (73C175.1.1) [NB100-56546] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Chk2 (73C175.1.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Immunocytochemistry-Immunofluorescence-NB100-56546-img0004.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) [NB100-56546] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Immunohistochemistry-Paraffin-NB100-56546-img0002.jpg)
![Flow Cytometry: Chk2 Antibody (73C175.1.1) [NB100-56546] - An intracellular stain was performed on Hek293 cells with Chk2 [73C175.1.1] Antibody NB100-56546 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Flow-Cytometry-NB100-56546-img0006.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Western-Blot-NB100-56546-img0001.jpg)
![Western Blot: SOX2 Antibody [NBP2-24390] - Analysis of SOX2 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/SOX2-Antibody-Western-Blot-NBP2-24390-img0002.jpg "Western Blot: SOX2 Antibody [NBP2-24390] - Analysis of SOX2 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: SOX2 Antibody [NBP2-24390] - FFPE human skin stained with SOX2 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/SOX2-Antibody-Immunohistochemistry-Paraffin-NBP2-24390-img0003.jpg)
![Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NB110-40660-img0002.jpg "Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB110-40660] - Staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes. Antibody at 5 ug/mL. Data kindly provided by CMRB, Center of Regenerative Medicine in Barcelona, Spain.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40660-img0001.jpg)
![Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates with Rabbit anti-Actin antibody at 1/500 dilution.Lane 1: Hela cell lysate (10 ug/Lane)Lane 2: A431 cell lysate (10 ug/Lane)Lane 3: PC-12 cell lysate (10 ug/Lane)Lane 4: Mouse brain tissue lysate (20 ug/Lane)Lane 5: Rat kidney tissue lysate (20 ug/Lane)Predicted band size: 42 kDaObserved band size: 42 kDaExposure time: 30 seconds;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-80) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Western-Blot-NBP2-67907-img0008.jpg "Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates with Rabbit anti-Actin antibody at 1/500 dilution.Lane 1: Hela cell lysate (10 ug/Lane)Lane 2: A431 cell lysate (10 ug/Lane)Lane 3: PC-12 cell lysate (10 ug/Lane)Lane 4: Mouse brain tissue lysate (20 ug/Lane)Lane 5: Rat kidney tissue lysate (20 ug/Lane)Predicted band size: 42 kDaObserved band size: 42 kDaExposure time: 30 seconds;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-80) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Actin Antibody (JJ09-29) [NBP2-67907] - Staining Actin in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunocytochemistry-Immunofluorescence-NBP2-67907-img0006.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (JJ09-29) [NBP2-67907] - Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Actin with Rabbit anti-Actin antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunohistochemistry-Paraffin-NBP2-67907-img0009.jpg)
![Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control: Lane 1: zebrafish Lane 2: Hela Lane 3: A431 Lane 4: Jurkat](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Western-Blot-NBP2-67907-img0005.jpg)
![Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control: Lane 1:Hybrid fish (crucian-carp) brain tissue Lane 2:Hybrid fish (crucian-carp) kidney tissue](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Western-Blot-NBP2-67907-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (JJ09-29) [NBP2-67907] - Staining Actin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunocytochemistry-Immunofluorescence-NBP2-67907-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (JJ09-29) [NBP2-67907] - Staining Actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunocytochemistry-Immunofluorescence-NBP2-67907-img0002.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of paraffin-embedded mouse cardiac muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunohistochemistry-Paraffin-NBP2-67907-img0003.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of paraffin-embedded mouse smooth muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunohistochemistry-Paraffin-NBP2-67907-img0004.jpg)