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![Western Blot: Tubulin alpha-1B Antibody [NBP2-20760] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Western-Blot-NBP2-20760-img0001.jpg "Western Blot: Tubulin alpha-1B Antibody [NBP2-20760] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE gel, diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody [NBP2-20760] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TUBA1B protein stained by alpha Tubulin antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20760-img0003.jpg)
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody [NBP2-20760] - Human colon carcinoma, using alpha Tubulin antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Immunohistochemistry-Paraffin-NBP2-20760-img0002.jpg)
![Immunohistochemistry: Tubulin alpha-1B Antibody [NBP2-20760] - Tubulin alpha-1B antibody detects Tubulin alpha-1B protein on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. Tubulin alpha-1B antibody (NBP2-20760) dilution: 1:200.](http://images.novusbio.com/fullsize/nbp2-20760_rabbit-polyclonal-tubulin-alpha-1b-antibody-91020241957217.jpg)
![Western Blot: Tubulin alpha-1B Antibody [NBP2-20760] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody (NBP2-20760) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-20760_rabbit-polyclonal-tubulin-alpha-1b-antibody-91020242001045.jpg)
![Western Blot: ATP5J Antibody [NBP2-15514] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa membrane 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ATP5J-Antibody-Western-Blot-NBP2-15514-img0001.jpg "Western Blot: ATP5J Antibody [NBP2-15514] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa membrane 15% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: ATP5J Antibody [NBP2-15514] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ATP5J-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15514-img0003.jpg)
![Immunohistochemistry-Paraffin: ATP5J Antibody [NBP2-15514] - Immunohistochemical analysis of paraffin-embedded J5 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ATP5J-Antibody-Immunohistochemistry-Paraffin-NBP2-15514-img0002.jpg)
![Western Blot: CYP27A1 Antibody [NBP2-16062] - Sample (50 ug of whole cell lysate) A: Mouse Liver. Antibody at 1:1000.](http://images.novusbio.com/fullsize/CYP27A1-Antibody-Western-Blot-NBP2-16062-img0008.jpg "Western Blot: CYP27A1 Antibody [NBP2-16062] - Sample (50 ug of whole cell lysate) A: Mouse Liver. Antibody at 1:1000.")
![Immunohistochemistry-Paraffin: CYP27A1 Antibody [NBP2-16062] - CYP27A1 antibody detects CYP27A1 protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis. CYP27A1 antibody at 1:500.](http://images.novusbio.com/fullsize/CYP27A1-Antibody-Immunohistochemistry-Paraffin-NBP2-16062-img0009.jpg)
![Western Blot: CYP27A1 Antibody [NBP2-16062] - Sample (30 ug of whole cell lysate) A: A549. Separation by 7.5% SDS-PAGE. Antibody at 1:1000.](http://images.novusbio.com/fullsize/CYP27A1-Antibody-Western-Blot-NBP2-16062-img0001.jpg)
![Western Blot: CYP27A1 Antibody [NBP2-16062] - A. 50 ug rat liver lysate/extract.](http://images.novusbio.com/fullsize/CYP27A1-Antibody-Western-Blot-NBP2-16062-img0007.jpg)
![Immunohistochemistry-Paraffin: CYP27A1 Antibody [NBP2-16062] - U87 xenograft, using CYP27A1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CYP27A1-Antibody-Immunohistochemistry-Paraffin-NBP2-16062-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: CYP27A1 Antibody [NBP2-16062] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16062_rabbit-polyclonal-cyp27a1-antibody-1610202419414588.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Immunocytochemistry-Immunofluorescence-DDX0141P-100-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone")
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - FACS staining of hCD40L-transfected Lcellswith LL2 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Flow-Cytometry-DDX0141P-100-img0002.jpg)
![Blocking/Neutralizing: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - LL2 inhibitsthe stimulatory effect of the CD40L-transfected L cells on synovial fibroblasts Rissoan et al, Clin Exp Immunol. 1996, 106(3):481-90.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Blocking-Neutralizing-DDX0141P-100-img0003.jpg)
![Western Blot: Tau Antibody [NBP2-25163] - Analysis of tissue lysates using chicken pAb to MAP-tau, NBP2-25163, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as multiple isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot in the range from 48 kDa to 67 kDa.](http://images.novusbio.com/fullsize/Tau-Antibody-Western-Blot-NBP2-25163-img0005.jpg "Western Blot: Tau Antibody [NBP2-25163] - Analysis of tissue lysates using chicken pAb to MAP-tau, NBP2-25163, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as multiple isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot in the range from 48 kDa to 67 kDa.")
![Immunocytochemistry/Immunofluorescence: Tau Antibody [NBP2-25163] - Immunofluorescent analysis of cortical neuron-glial culture from E20 rat stained with chicken pAb to MAP-tau, NBP2-25163, dilution 1:2,000 in green, and costained with mouse mAb to MAP2, dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. The NBP2-25163 antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both MAP2 and tau proteins.](http://images.novusbio.com/fullsize/Tau-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25163-img0004.jpg)
![Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-05933] - Detection of AGR2 in mouse prostate.](http://images.novusbio.com/fullsize/AG-2-Antibody-Immunohistochemistry-Paraffin-NBP1-05933-img0006.jpg "Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-05933] - Detection of AGR2 in mouse prostate.")
![Immunohistochemistry: AG-2 Antibody [NBP1-05933] - Detection of AGR2 in mouse kidney.](http://images.novusbio.com/fullsize/AG-2-Antibody-Immunohistochemistry-NBP1-05933-img0005.jpg)
![Western Blot: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Detection of Human and Mouse CSE1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSE1 antibody NB100-79803 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CSE1L-CAS-Exportin-2-Antibody-Western-Blot-NB100-79803-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Detection of Human and Mouse CSE1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSE1 antibody NB100-79803 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Mouse plasmacytoma. Antibody: Affinity purified rabbit anti-CSE1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CSE1L-CAS-Exportin-2-Antibody-Immunohistochemistry-Paraffin-NB100-79803-img0010.jpg)
![Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Human testicular seminoma. Antibody: Affinity purified rabbit anti-CSE1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CSE1L-CAS-Exportin-2-Antibody-Immunohistochemistry-Paraffin-NB100-79803-img0009.jpg)
![Immunoprecipitation: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (R; 50 mcg) cells. B. Whole cell lysate (1 mg for IP, 1/4 of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-CSE1L/CAS/Exportin-2 antibody BL1975 (Cat. No. NB100-79803) used at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) for WB and at 3 mcg/mg lysate for IP. Detection: Chemiluminescence with an exposure time of 3 minutes (A and B).](http://images.novusbio.com/fullsize/CSE1L-CAS-Exportin-2-Antibody-Immunoprecipitation-NB100-79803-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Detection of human CSE1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSE1 antibody NB100-79803 (lot NB100-79803-2) used for IP at 6 ug per reaction. CSE1 was also immunoprecipitated by a previous lot of this antibody (NB100-79803-1) and another rabbit anti-CSE1 antibody. For blotting immunoprecipitated CSE1, NB100-79803 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CSE1L-CAS-Exportin-2-Antibody-Immunoprecipitation-NB100-79803-img0013.jpg)
![Western Blot: CRABP2 Antibody [NBP2-24764] - Analysis in HCT116 cell lysate in the 1) absence and 2) presence of immunizing peptide. Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Western-Blot-NBP2-24764-img0002.jpg "Western Blot: CRABP2 Antibody [NBP2-24764] - Analysis in HCT116 cell lysate in the 1) absence and 2) presence of immunizing peptide. Dilution: 1.0 ug/ml.")
![Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-24764] - Staining of human liver tissue using an isotype control (left) and this antibody (right) at 5 ug/ml.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Immunohistochemistry-Paraffin-NBP2-24764-img0001.jpg)
![Western Blot: CRABP2 Antibody [NBP2-15998] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CRABP2 antibody [N1C3] diluted at 1:1000.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Western-Blot-NBP2-15998-img0005.jpg "Western Blot: CRABP2 Antibody [NBP2-15998] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CRABP2 antibody [N1C3] diluted at 1:1000.")
![Western Blot: CRABP2 Antibody [NBP2-15998] - Sample (30 ug of whole cell lysate) A: Hep G2 15% SDS PAGE NBP2-15998 diluted at 1:1000.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Western-Blot-NBP2-15998-img0001.jpg)
![Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-15998] - Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Immunohistochemistry-Paraffin-NBP2-15998-img0002.jpg)
![CRABP2 antibody [N1C3] detects CRABP2 protein expression by immunohistochemical analysis.Sample:Paraffin-Embedded adult mouse retina. Green: CRABP2 protein stained by CRABP2 antibody [N1C3] (NBP2-15998) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15998_rabbit-polyclonal-crabp2-antibody-immunohistochemistry-paraffin-261120251847573.jpg)
![CRABP2 antibody [N1C3] detects CRABP2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CRABP2 protein stained by CRABP2 antibody [N1C3] (NBP2-15998) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15998_rabbit-polyclonal-crabp2-antibody-immunocytochemistry-immunofluorescence-261120251849134.jpg)
![Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FKBP4 antibody.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Western-Blot-NBP1-33702-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FKBP4 antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FKBP52/FKBP4 Antibody [NBP1-33702] - Paraformaldehyde-fixed A431, using (NBP1-33702) antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33702-img0008.jpg)
![Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - A. 30 ug GL261 whole cell lysate/extract, B. 30 ug C8D30 whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Western-Blot-NBP1-33702-img0011.jpg)
![Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - 30 ug Rat 2 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Western-Blot-NBP1-33702-img0012.jpg)
![Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - A. 30 ug 293T whole cell lysate/extract, B. 30 ug A431 whole cell lysate/extract, C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A37C whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Western-Blot-NBP1-33702-img0013.jpg)
![Western Blot: Septin-8 Antibody [NBP2-15116] - Various tissue extracts (10 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Septin-8 antibody diluted at 1:4000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Septin-8-Antibody-Western-Blot-NBP2-15116-img0005.jpg "Western Blot: Septin-8 Antibody [NBP2-15116] - Various tissue extracts (10 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Septin-8 antibody diluted at 1:4000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Septin-8 Antibody [NBP2-15116] - Paraffin-embedded U87 xenograft; antibody dilution 1:500.](http://images.novusbio.com/fullsize/Septin-8-Antibody-Immunohistochemistry-Paraffin-NBP2-15116-img0002.jpg)
![Western Blot: Septin-8 Antibody [NBP2-15116] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/Septin-8-Antibody-Western-Blot-NBP2-15116-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Septin-8 Antibody [NBP2-15116] - Analysis of methanol-fixed HeLa, using 39332 antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Septin-8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15116-img0001.jpg)
![SEPT8 antibody detects SEPT8 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: SEPT8 protein stained by SEPT8 antibody (NBP2-15116) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15116_rabbit-polyclonal-septin-8-antibody-immunocytochemistry-immunofluorescence-261120251971820.jpg)
![Western Blot: MBD2 Antibody [NB100-81657] - Detection of Human and Mouse MBD2 on HeLa whole cell lysate using NB100-81657. MBD2 was also immunoprecipitated by rabbit anti-MBD2 antibody NB100-81656.](http://images.novusbio.com/fullsize/MBD2-Antibody-Western-Blot-NB100-81657-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: MBD2 Antibody [NB100-81657] - Detection of Human and Mouse MBD2 on HeLa whole cell lysate using NB100-81657. MBD2 was also immunoprecipitated by rabbit anti-MBD2 antibody NB100-81656.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-81657] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MBD2 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MBD2-Antibody-Immunohistochemistry-NB100-81657-img0012.jpg)