| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25144SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56660SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92688SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44249SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90062-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44338 | |||
| Azide and BSA Free | NBP2-34732-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48330 | |||
| Azide and BSA Free | NBP3-11580 | |||
![Western Blot: Aldolase C Antibody (1A1) [NBP2-25144] - Blots of crude rat brain lysates. The 1A1 clone binds strongly and cleanly to a band at about 40 kDa.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-1A1-Western-Blot-NBP2-25144-img0001.jpg "Western Blot: Aldolase C Antibody (1A1) [NBP2-25144] - Blots of crude rat brain lysates. The 1A1 clone binds strongly and cleanly to a band at about 40 kDa.")
![Immunocytochemistry/Immunofluorescence: Aldolase C Antibody (1A1) [NBP2-25144] - View of mixed neuron/glial cultures stained with NBP2-25144 (green) and the rabbit antibody to RBFOX3/NeuN (NBP1-92716, red). NBP2-25144 reveals strong cytoplasmic staining in astrocytes, while the rabbit RBFOX3/NeuN antibody shows nuclear and distal cytoplasmic staining in neuron cells and complete absence of astrocytes. Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-1A1-Immunocytochemistry-Immunofluorescence-NBP2-25144-img0003.jpg)
![Flow Cytometry: Aldolase C Antibody (1A1) [NBP2-25144] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Aldolase C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-1A1-Flow-Cytometry-NBP2-25144-img0004.jpg)
![Western Blot: RPL13A Antibody [NBP1-32710] - Sample (30 ug of whole cell lysate)A: zebrafish heart12% SDS PAGE diluted at 1:500](http://images.novusbio.com/fullsize/RPL13A-Antibody-Western-Blot-NBP1-32710-img0007.jpg "Western Blot: RPL13A Antibody [NBP1-32710] - Sample (30 ug of whole cell lysate)A: zebrafish heart12% SDS PAGE diluted at 1:500")
![Immunocytochemistry/Immunofluorescence: RPL13A Antibody [NBP1-32710] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/RPL13A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32710-img0003.jpg)
![Immunohistochemistry-Paraffin: RPL13A Antibody [NBP1-32710] - Analysis of paraffin-embedded zebrafish tissue, using RPL13A antibody [N1C3] at 1:300 dilution.](http://images.novusbio.com/fullsize/RPL13A-Antibody-Immunohistochemistry-Paraffin-NBP1-32710-img0006.jpg)
![Western Blot: RPL13A Antibody [NBP1-32710] - Sample (30 ug of whole cell lysate)A:293T B:HeLa10% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/RPL13A-Antibody-Western-Blot-NBP1-32710-img0005.jpg)
![Immunohistochemistry-Paraffin: RPL13A Antibody [NBP1-32710] - NCI-N87 xenograft, using RPL13A antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RPL13A-Antibody-Immunohistochemistry-Paraffin-NBP1-32710-img0004.jpg)
![Western Blot: NIFK Antibody [NB100-56660] - Analysis of NIFK in 30 ugs of HeLa cell lysate using NB100-56660 at 1:1000.](http://images.novusbio.com/fullsize/NIFK-Antibody-Western-Blot-NB100-56660-img0004.jpg "Western Blot: NIFK Antibody [NB100-56660] - Analysis of NIFK in 30 ugs of HeLa cell lysate using NB100-56660 at 1:1000.")
![Immunohistochemistry: NIFK Antibody [NB100-56660] - Staining in adipocytes of human carcinomatus breast tissue using NB100-56660 at 1:500 dilution.](http://images.novusbio.com/fullsize/NIFK-Antibody-Immunohistochemistry-NB100-56660-img0002.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Knockout-Validated-NBP1-92688-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2A52) [NBP1-92688] - Immunocytochemistry/Immunofluorescence: [NBP1-92688] - View of mixed neuron/glial cultures stained with NBP1-92687 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Immunocytochemistry-Immunofluorescence-NBP1-92688-img0005.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Analysis of cell and whole brain tissue lysates using mouse mAb to vimentin, NBP1-92688, dilution 1:5,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, [6] C6, and [7] rat brain. The band at about 50kDa mark corresponds to vimentin protein. The antibody does not recognize mouse vimentin (not shown).](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Western-Blot-NBP1-92688-img0006.jpg)
![Western Blot: PHGDH Antibody [NBP2-19801] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N3C2], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP2-19801-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PHGDH Antibody [NBP2-19801] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N3C2], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: PHGDH Antibody [NBP2-19801] - Sample (30 ug of whole cell lysate) A: A431 B: Raji 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP2-19801-img0001.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP2-19801] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP2-19801-img0002.jpg)
![PHGDH antibody [N3C2], Internal detects PHGDH protein by immunohistochemical analysis.Sample: Frozen-sectioned rat E13.5 brain.Green: PHGDH stained by PHGDH antibody [N3C2], Internal (NBP2-19801) diluted at 1:250.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19801_rabbit-polyclonal-phgdh-antibody-immunohistochemistry-frozen-2611202518503014.jpg)
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0005.jpg "Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.")
![Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human breast carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0006.jpg)
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Plk-1 pT210 antibody to detect endogenous protein present in a Mouse A20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~68 kDa corresponding to Plk-1 protein. It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0002.jpg)
![Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0007.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody [NBP1-33611] - Paraffin-embedded mouse brain. Presenilin 1 antibody [C1C2], Internal dilution: 1:500.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33611-img0011.jpg "Immunohistochemistry-Paraffin: Presenilin-1 Antibody [NBP1-33611] - Paraffin-embedded mouse brain. Presenilin 1 antibody [C1C2], Internal dilution: 1:500.")
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody [NBP1-33611] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33611-img0008.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody [NBP1-33611] - Paraffin-embedded U87 xenograft. Presenilin 1 antibody [C1C2], Internal dilution: 1:500.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33611-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody [NBP1-33611] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Presenilin 1 stained by Presenilin 1 antibody [C1C2], Internal diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/antibody/nbp1-33611_rabbit-polyclonal-presenilin-1-antibody-immunocytochemistry-immunofluorescence-562023123542..jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Presenilin-1 Antibody [NBP1-33611] - Wild-type (WT) and Presenilin 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Presenilin 1 antibody [C1C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-33611_rabbit-polyclonal-presenilin-1-antibody-1610202419414521.jpg)
![Western Blot: CD133 Antibody [NBP2-44249] - Total mouse placenta protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/ml anti-mCD133 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CD133-Antibody-Western-Blot-NBP2-44249-img0001.jpg "Western Blot: CD133 Antibody [NBP2-44249] - Total mouse placenta protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/ml anti-mCD133 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CD133 Antibody [NBP2-44249] - NIH/3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-mCD133 (M30) (NBP2-44249) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44249-img0002.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Staining of human cerebellum, cerebral cortex, skeletal muscle and testis using Anti-Ataxin-2 antibody NBP1-90062 (A) shows similar protein distribution across tissues to independent antibody NBP1-90063 (B).](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90062-img0017.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Staining of human cerebellum, cerebral cortex, skeletal muscle and testis using Anti-Ataxin-2 antibody NBP1-90062 (A) shows similar protein distribution across tissues to independent antibody NBP1-90063 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Analysis in human testis and skeletal muscle tissues. Corresponding Ataxin-2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90062-img0013.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90062-img0018.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90062-img0014.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90062-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Transketolase Antibody [NBP2-20699] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Transketolase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20699-img0002.jpg "Immunocytochemistry/Immunofluorescence: Transketolase Antibody [NBP2-20699] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.")
![Western Blot: Transketolase Antibody [NBP2-20699] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Transketolase-Antibody-Western-Blot-NBP2-20699-img0001.jpg)
![Transketolase antibody [N3C2], Internal detects Transketolase protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Transketolase antibody [N3C2], Internal (NBP2-20699) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20699_rabbit-polyclonal-transketolase-antibody-immunohistochemistry-paraffin-261120251848233.jpg)
![Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Transketolase antibody [N3C2], Internal (NBP2-20699) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-20699_rabbit-polyclonal-transketolase-antibody-western-blot-261120251819367.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Transketolase antibody [N3C2], Internal (NBP2-20699) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-20699_rabbit-polyclonal-transketolase-antibody-western-blot-2611202519623.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (SPM122) [NBP2-32930] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD31/PECAM-1 Antibody (SPM122).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-SPM122-Immunohistochemistry-Paraffin-NBP2-32930-img0001.jpg "Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (SPM122) [NBP2-32930] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD31/PECAM-1 Antibody (SPM122).")
![Flow Cytometry: CD31/PECAM-1 Antibody (SPM122) [NBP2-32930] - Flow Cytometric analysis of human CD31/PECAM-1 on Jurkat cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled CD31/PECAM-1 Monoclonal Antibody (SPM122).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-SPM122-Flow-Cytometry-NBP2-32930-img0002.jpg)
![Western Blot: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-10-Western-Blot-NBP2-44340-img0005.jpg "Western Blot: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.10).")
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Human Tonsil stained with CD31/PECAM-1 Antibody (C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-10-Immunohistochemistry-Paraffin-NBP2-44340-img0003.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Flow Cytometry of human CD31 on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled CD31/PECAM-1 Antibody (C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-10-Flow-Cytometry-NBP2-44340-img0004.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Human Angiosarcoma stained with CD31/PECAM-1 Antibody (C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-10-Immunohistochemistry-Paraffin-NBP2-44340-img0002.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Human CD31 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled CD31/PECAM-1 Antibody (C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-10-Flow-Cytometry-NBP2-44340-img0001.jpg)