| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56359SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB35062-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56660SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92688SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25144SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90062-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44249SS | |||
![Western Blot: APE Antibody [NB100-56359] - Analysis of APE in HeLa lysates using APE antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/APE-Antibody-Western-Blot-NB100-56359-img0005.jpg "Western Blot: APE Antibody [NB100-56359] - Analysis of APE in HeLa lysates using APE antibody at 2 ug/mL.")
![Immunohistochemistry-Paraffin: APE Antibody [NB100-56359] - Human Breast: FFPE tissue section using APE antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/APE-Antibody-Immunohistochemistry-Paraffin-NB100-56359-img0004.jpg)
![Western Blot: RUVBL2 Antibody [NBP1-33599] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Western-Blot-NBP1-33599-img0004.jpg "Western Blot: RUVBL2 Antibody [NBP1-33599] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: RUVBL2 Antibody [NBP1-33599] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33599-img0003.jpg)
![Immunohistochemistry-Paraffin: RUVBL2 Antibody [NBP1-33599] - Mouse testis.RUVBL2 antibody [N1C1] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Immunohistochemistry-Paraffin-NBP1-33599-img0006.jpg)
![Immunoprecipitation of RUVBL2 protein from 293T whole cell extracts using 5 ug of RUVBL2 antibody [N1C1] (NBP1-33599).Western blot analysis was performed using RUVBL2 antibody [N1C1] (NBP1-33599).EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/nbp1-33599_rabbit-polyclonal-ruvbl2-antibody-immunoprecipitation-2611202518491321.jpg)
![Western Blot: Cytokeratin 19 Antibody (963410) [MAB35062] - Western blot detection of Cytokeratin 19 (clone 963410), catalog number MAB35062, in HT-29, ZR-75, DU145 and MCF-7 cell lysates at a dilution of 0.25 ug/ml.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-963410-Western-Blot-MAB35062-img0003.jpg "Western Blot: Cytokeratin 19 Antibody (963410) [MAB35062] - Western blot detection of Cytokeratin 19 (clone 963410), catalog number MAB35062, in HT-29, ZR-75, DU145 and MCF-7 cell lysates at a dilution of 0.25 ug/ml.")
![Immunohistochemistry: Cytokeratin 19 Antibody (963410) [MAB35062] - Cytokeratin 19 was detected in immersion fixed paraffin-embedded sections of human breast cancer using Cytokeratin 19 (963410) Monoclonal Antibody at 5 ug/ml. Tissue was stained using Mouse IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in cancer cells.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-963410-Immunohistochemistry-MAB35062-img0006.jpg)
![Western Blot: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Proteasome 20S alpha 5 antibody detects Proteasome 20S alpha 5 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Proteasome 20S alpha 5 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/Proteasome-20S-alpha-5-Antibody-Western-Blot-NBP1-32387-img0008.jpg "Western Blot: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Proteasome 20S alpha 5 antibody detects Proteasome 20S alpha 5 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Proteasome 20S alpha 5 antibody diluted by 1:1000.")
![Immunocytochemistry/Immunofluorescence: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Proteasome-20S-alpha-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32387-img0003.jpg)
![Immunohistochemistry-Paraffin: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Proteasome 20S alpha 5 antibody detects Proteasome 20S alpha 5 protein at cytoplasm and nucleus in human hepatocellular carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. Proteasome 20S alpha 5 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Proteasome-20S-alpha-5-Antibody-Immunohistochemistry-Paraffin-NBP1-32387-img0007.jpg)
![Western Blot: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/Proteasome-20S-alpha-5-Antibody-Western-Blot-NBP1-32387-img0005.jpg)
![Immunohistochemistry-Paraffin: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Proteasome-20S-alpha-5-Antibody-Immunohistochemistry-Paraffin-NBP1-32387-img0004.jpg)
![Western Blot: NIFK Antibody [NB100-56660] - Analysis of NIFK in 30 ugs of HeLa cell lysate using NB100-56660 at 1:1000.](http://images.novusbio.com/fullsize/NIFK-Antibody-Western-Blot-NB100-56660-img0004.jpg "Western Blot: NIFK Antibody [NB100-56660] - Analysis of NIFK in 30 ugs of HeLa cell lysate using NB100-56660 at 1:1000.")
![Immunohistochemistry: NIFK Antibody [NB100-56660] - Staining in adipocytes of human carcinomatus breast tissue using NB100-56660 at 1:500 dilution.](http://images.novusbio.com/fullsize/NIFK-Antibody-Immunohistochemistry-NB100-56660-img0002.jpg)
![Western Blot: TRIP6 Antibody [NB100-79791] - Detection of Human TRIP6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP6 antibody NB100-79791 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/TRIP6-Antibody-Western-Blot-NB100-79791-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: TRIP6 Antibody [NB100-79791] - Detection of Human TRIP6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP6 antibody NB100-79791 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: TRIP6 Antibody [NB100-79791] - Nuclear extract (NE) from HeLa cells. Antibody used at 0.3 ug/ml.](http://images.novusbio.com/fullsize/TRIP6-Antibody-Western-Blot-NB100-79791-img0003.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Knockout-Validated-NBP1-92688-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2A52) [NBP1-92688] - Immunocytochemistry/Immunofluorescence: [NBP1-92688] - View of mixed neuron/glial cultures stained with NBP1-92687 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Immunocytochemistry-Immunofluorescence-NBP1-92688-img0005.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Analysis of cell and whole brain tissue lysates using mouse mAb to vimentin, NBP1-92688, dilution 1:5,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, [6] C6, and [7] rat brain. The band at about 50kDa mark corresponds to vimentin protein. The antibody does not recognize mouse vimentin (not shown).](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Western-Blot-NBP1-92688-img0006.jpg)
![Western Blot: RUVBL1 Antibody [NBP2-27147] - analysis of Rvb1 in human testis lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse testis and 4) rat testis lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Western-Blot-NBP2-27147-img0002.jpg "Western Blot: RUVBL1 Antibody [NBP2-27147] - analysis of Rvb1 in human testis lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse testis and 4) rat testis lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-27147] - Immunohistochemical analysis of Rvb1 in formalin-fixed, paraffin-embedded mouse testis tissue using NBP2-27147 at 5 ug/ml.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-27147-img0003.jpg)
![Western Blot: Aldolase C Antibody (1A1) [NBP2-25144] - Blots of crude rat brain lysates. The 1A1 clone binds strongly and cleanly to a band at about 40 kDa.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-1A1-Western-Blot-NBP2-25144-img0001.jpg "Western Blot: Aldolase C Antibody (1A1) [NBP2-25144] - Blots of crude rat brain lysates. The 1A1 clone binds strongly and cleanly to a band at about 40 kDa.")
![Immunocytochemistry/Immunofluorescence: Aldolase C Antibody (1A1) [NBP2-25144] - View of mixed neuron/glial cultures stained with NBP2-25144 (green) and the rabbit antibody to RBFOX3/NeuN (NBP1-92716, red). NBP2-25144 reveals strong cytoplasmic staining in astrocytes, while the rabbit RBFOX3/NeuN antibody shows nuclear and distal cytoplasmic staining in neuron cells and complete absence of astrocytes. Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-1A1-Immunocytochemistry-Immunofluorescence-NBP2-25144-img0003.jpg)
![Flow Cytometry: Aldolase C Antibody (1A1) [NBP2-25144] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Aldolase C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-1A1-Flow-Cytometry-NBP2-25144-img0004.jpg)
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0005.jpg "Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.")
![Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human breast carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0006.jpg)
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Plk-1 pT210 antibody to detect endogenous protein present in a Mouse A20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~68 kDa corresponding to Plk-1 protein. It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0002.jpg)
![Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0007.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Staining of human cerebellum, cerebral cortex, skeletal muscle and testis using Anti-Ataxin-2 antibody NBP1-90062 (A) shows similar protein distribution across tissues to independent antibody NBP1-90063 (B).](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90062-img0017.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Staining of human cerebellum, cerebral cortex, skeletal muscle and testis using Anti-Ataxin-2 antibody NBP1-90062 (A) shows similar protein distribution across tissues to independent antibody NBP1-90063 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Analysis in human testis and skeletal muscle tissues. Corresponding Ataxin-2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90062-img0013.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90062-img0018.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90062-img0014.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90062-img0016.jpg)
![Western Blot: CD133 Antibody [NBP2-44249] - Total mouse placenta protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/ml anti-mCD133 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CD133-Antibody-Western-Blot-NBP2-44249-img0001.jpg "Western Blot: CD133 Antibody [NBP2-44249] - Total mouse placenta protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/ml anti-mCD133 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CD133 Antibody [NBP2-44249] - NIH/3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-mCD133 (M30) (NBP2-44249) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44249-img0002.jpg)