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![Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Anti-TNIK antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/TNIK-Antibody-Immunohistochemistry-Paraffin-NLS7401-img0006.jpg "Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Anti-TNIK antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Analysis of anti-TNIK antibody with kidney at 2 ug/ml.](http://images.novusbio.com/fullsize/TNIK-Antibody-Immunohistochemistry-Paraffin-NLS7401-img0002.jpg)
![Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Analysis of anti-TNIK antibody with human breast, carcinoma.](http://images.novusbio.com/fullsize/TNIK-Antibody-Immunohistochemistry-Paraffin-NLS7401-img0003.jpg)
![Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Anti-TNIK antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/TNIK-Antibody-Immunohistochemistry-Paraffin-NLS7401-img0004.jpg)
![Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Anti-TNIK antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/TNIK-Antibody-Immunohistochemistry-Paraffin-NLS7401-img0005.jpg)
![Western Blot: Cofilin Antibody (PSH01-44) [NBP3-32207] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (30 ug/Lane) Lane 5: SH-SY5Y cell lysate (30 ug/Lane) Lane 6: HUVEC cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: COS-1 cell lysate (30 ug/Lane) Lane 9: VERO cell lysate (30 ug/Lane) Lane 10: NIH/3T3 cell lysate (30 ug/Lane) Lane 11: PC-12 cell lysate (30 ug/Lane) Lane 12: Neuro-2a cell lysate (30 ug/Lane) Lane 13: K-562 cell lysate (30 ug/Lane) Lane 14: A431 cell lysate (30 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32207) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-278202416285276.jpg "Western Blot: Cofilin Antibody (PSH01-44) [NBP3-32207] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (30 ug/Lane) Lane 5: SH-SY5Y cell lysate (30 ug/Lane) Lane 6: HUVEC cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: COS-1 cell lysate (30 ug/Lane) Lane 9: VERO cell lysate (30 ug/Lane) Lane 10: NIH/3T3 cell lysate (30 ug/Lane) Lane 11: PC-12 cell lysate (30 ug/Lane) Lane 12: Neuro-2a cell lysate (30 ug/Lane) Lane 13: K-562 cell lysate (30 ug/Lane) Lane 14: A431 cell lysate (30 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32207) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cofilin Antibody (PSH01-44) [NBP3-32207] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32207) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-2982024142099.jpg)
![Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (PSH01-44) [NBP3-32207] - Immunocytochemistry analysis of HeLa cells labeling Cofilin with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-2920241048483.jpg)
![Flow Cytometry: Cofilin Antibody (PSH01-44) [NBP3-32207] - Flow cytometric analysis of HeLa cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32207, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-29202416372168.jpg)
![Immunohistochemistry-Paraffin: CrkL Antibody [NB300-884] - Paraffin embedded human thymus. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CrkL-Antibody-Immunohistochemistry-Paraffin-NB300-884-img0001.jpg "Immunohistochemistry-Paraffin: CrkL Antibody [NB300-884] - Paraffin embedded human thymus. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: CrkL Antibody [NB300-884] - (0.1ug/ml) staining of K562 (A) and (1ug/ml) Daudi (B) and NIH3T3 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb300-884_goat-polyclonal-crkl-antibody-1652023918333.jpg)
![Flow Cytometry: CrkL Antibody [NB300-884] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb300-884_goat-polyclonal-crkl-antibody-1652023918337.jpg)
![Immunocytochemistry/Immunofluorescence: CrkL Antibody [NB300-884] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb300-884_goat-polyclonal-crkl-antibody-16520239183315.jpg)
![Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of paraffin-embedded human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-immunohistochemistry-paraffin-168202375610..jpg "Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of paraffin-embedded human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.")
![Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)- Human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-immunohistochemistry-paraffin-16820237586..jpg)
![Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved NIH-3T3 treated with PDGFA 5 ug/mL, 5 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:1000, 1:50000, 1:200000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-168202375944.jpg)
![<b>Biological Strategies Validation. </b>Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved A431, untreated (-); treated with EGF (200 ng/mL, 15 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution (upper) or anti-Akt antibody (lower).](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-16820238120.jpg)
![Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5 ug/mL, 5 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-16820238442..jpg)
![Western Blot: AKT1 [p Ser473] Antibody (0113) [NBP3-21480] - Western blot analysis of extracts from serum-starved NIH-3T3 treated with PDGFA (5 ug/mL, 5 min; +), using NBP3-21480 at 1:1000, 1:50000, 1:200000 dilution.](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser473-mab-0113-136202422345072.png)
![Western Blot: AKT1 [p Ser473] Antibody (0113) [NBP3-21480] - Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5000 ng/mL, 5min; +) (line B); treated with PDGFA and -phosphatase (line C) using NBP3-21480 at 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser473-mab-0113-23520251628115.png)
![Western Blot: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Western blot analysis of RelA/NFkB p65 on different lysates with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/500 dilution. Lane 1: Hela cell lysate (control) Lane 2: Hela-RELA-KO (1) Lane 3: Hela-RELA-KO (2) Lysates/proteins at 35 ug/Lane. Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32648) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-278202415212453.jpg "Western Blot: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Western blot analysis of RelA/NFkB p65 on different lysates with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/500 dilution. Lane 1: Hela cell lysate (control) Lane 2: Hela-RELA-KO (1) Lane 3: Hela-RELA-KO (2) Lysates/proteins at 35 ug/Lane. Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32648) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32648) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-29820241435276.jpg)
![Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Immunocytochemistry analysis of NIH/3T3 cells treated with or without 50μg/mL TNF-alpha for 20 minutes labeling RelA/NFkB p65 with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-29202410484811.jpg)
![Flow Cytometry: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Flow cytometric analysis of NIH/3T3 cells labeling RelA/NFkB p65. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32648, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-2920241637214.jpg)
![Immunocytochemistry/Immunofluorescence: TrkC Antibody [NBP2-15185] - TrkC antibody was tested in neuro2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/TrkC-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15185-img0001.jpg "Immunocytochemistry/Immunofluorescence: TrkC Antibody [NBP2-15185] - TrkC antibody was tested in neuro2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry: TrkC Antibody [NBP2-15185] - TrkC antibody was tested in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/TrkC-Antibody-Immunohistochemistry-NBP2-15185-img0003.jpg)
![Immunoprecipitation: PTP1B/PTPN1 Antibody [NBP1-28691] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PTPN1 antibody NBP1-28691 used for IP at 10 ug/mg lysate. PTPN1 was also immunoprecipitated by rabbit anti-PTPN1 antibody NBP1-28692, which recognizes a downstream epitope. For blotting immunoprecipitated PTPN1, NBP1-28691 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/PTP1B-PTPN1-Antibody-Immunoprecipitation-NBP1-28691-img0001.jpg "Immunoprecipitation: PTP1B/PTPN1 Antibody [NBP1-28691] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PTPN1 antibody NBP1-28691 used for IP at 10 ug/mg lysate. PTPN1 was also immunoprecipitated by rabbit anti-PTPN1 antibody NBP1-28692, which recognizes a downstream epitope. For blotting immunoprecipitated PTPN1, NBP1-28691 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Western Blot: ATP Citrate Lyase Antibody [NBP2-15510] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATP Citrate Lyase Antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Western-Blot-NBP2-15510-img0005.jpg "Western Blot: ATP Citrate Lyase Antibody [NBP2-15510] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATP Citrate Lyase Antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ATP Citrate Lyase Antibody [NBP2-15510] - ATP Citrate Lyase Antibody detects ATP Citrate Lyase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ATP Citrate Lyase protein stained by ATP Citrate Lyase Antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15510-img0004.jpg)
![Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP2-15510] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP2-15510-img0002.jpg)
![Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP2-15510] - ATP Citrate Lyase antibody [N1N2], N-term detects ATP Citrate Lyase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse pancreas.ATP Citrate Lyase stained by ATP Citrate Lyase antibody [N1N2], N-term (NBP2-15510) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15510_rabbit-polyclonal-atp-citrate-lyase-antibody-91020242091493.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATP citrate lyase antibody [N1N2], N-term (NBP2-15510) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-15510_rabbit-polyclonal-atp-citrate-lyase-antibody-western-blot-26112025195721.jpg)
![Western Blot: HDAC7 [p Ser155] Antibody [NBP2-24454] - Analysis of phosphorylated HDAC7 in (A) recombinant fusion protein containing a phosphorylated serine at position 155and (B) fusion protein containinug an unphosphorylated serine at position 155, using NBP2-24454 at 0.1 ug/ml.](http://images.novusbio.com/fullsize/HDAC7-[p-Ser155]-Antibody-Western-Blot-NBP2-24454-img0003.jpg "Western Blot: HDAC7 [p Ser155] Antibody [NBP2-24454] - Analysis of phosphorylated HDAC7 in (A) recombinant fusion protein containing a phosphorylated serine at position 155and (B) fusion protein containinug an unphosphorylated serine at position 155, using NBP2-24454 at 0.1 ug/ml.")
![Western Blot: MLF1 Interacting Protein [p Thr78] Antibody [NBP1-77938] - Shows detection of MLF1IP phosphorylated at Thr78. HeLa cells were co-infected with the indicated adenoviruses expressing GFP-tagged Plk1 or PBIP1. Blots were probed with the anti-MLF1IP pT78 antibody, stripped, and then reprobed with anti-GFP antibody (KA/G & Park, et al., 2006).](http://images.novusbio.com/fullsize/MLF1-Interacting-Protein-[p-Thr78]-Antibody-Western-Blot-NBP1-77938-img0004.jpg "Western Blot: MLF1 Interacting Protein [p Thr78] Antibody [NBP1-77938] - Shows detection of MLF1IP phosphorylated at Thr78. HeLa cells were co-infected with the indicated adenoviruses expressing GFP-tagged Plk1 or PBIP1. Blots were probed with the anti-MLF1IP pT78 antibody, stripped, and then reprobed with anti-GFP antibody (KA/G & Park, et al., 2006).")
![Immunohistochemistry: MLF1 Interacting Protein [p Thr78] Antibody [NBP1-77938] - Used at 20 g/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderately positive staining of mitotic cells in colon adenocarcinoma at 60X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.](http://images.novusbio.com/fullsize/MLF1-Interacting-Protein-[p-Thr78]-Antibody-Immunohistochemistry-NBP1-77938-img0007.jpg)
![Immunohistochemistry: MLF1 Interacting Protein [p Thr78] Antibody [NBP1-77938] - Analysis of MLF1IP pT78 at the kinetochores of HeLa cells in different phases of the cell cycle. Fluorescent signals were detectable at the kinetochores as early as G2, became most abundant in prophase cells with a discernible nuclear envelope, and gradually diminished as cells proceeded through mitosis (Kang & Park, et al., 2006).](http://images.novusbio.com/fullsize/MLF1-Interacting-Protein-[p-Thr78]-Antibody-Immunohistochemistry-NBP1-77938-img0006.jpg)
![Western Blot: HDAC7 [p Ser448] Antibody [NBP2-03979] - Analysis of phosphorylated HDAC-7 in (A) recombinant fusion protein containing Ser448 and (B) fusion protein without the phosphorylated amino acid, using this antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/HDAC7-[p-Ser448]-Antibody-Western-Blot-NBP2-03979-img0002.jpg "Western Blot: HDAC7 [p Ser448] Antibody [NBP2-03979] - Analysis of phosphorylated HDAC-7 in (A) recombinant fusion protein containing Ser448 and (B) fusion protein without the phosphorylated amino acid, using this antibody at 1 ug/ml.")
![Western Blot: HGS Antibody [NB100-55249] - Detection of Human HRS by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody NB100-55249 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/HGS-Antibody-Western-Blot-NB100-55249-img0002.jpg "Western Blot: HGS Antibody [NB100-55249] - Detection of Human HRS by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody NB100-55249 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: HGS Antibody [NB100-55249] - Whole cell lysate (15 ug) from Renca, CT26.WT and 4T1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/HGS-Antibody-Western-Blot-NB100-55249-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: HGS Antibody [NB100-55249] - Detection of human HRS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HRS antibody NB100-55249 (lot NB100-55249-2) used for IP at 6 ug per reaction. HRS was also immunoprecipitated by a previous lot of this antibody (NB100-55249-1) and another rabbit anti-HRS antibody. For blotting immunoprecipitated HRS, NB100-55249 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/HGS-Antibody-Immunoprecipitation-NB100-55249-img0004.jpg)