| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03748-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93989-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94511-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61775-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59700-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52520-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61782-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61834-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59326-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50537-0.025ml | |||
![Western Blot: Kv4.2 Antibody - BSA Free [NBP3-03748] - Western blot analysis of Mouse heart, using Kv4.2 antibody (A6203) at 1:3000 dilution.<br>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.<br>Lysates/proteins: 25μg per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit (RM00020).<br>Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-03748_rabbit-polyclonal-kv4-2-antibody-5220251931375.jpg "Western Blot: Kv4.2 Antibody - BSA Free [NBP3-03748] - Western blot analysis of Mouse heart, using Kv4.2 antibody (A6203) at 1:3000 dilution.<br>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.<br>Lysates/proteins: 25μg per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit (RM00020).<br>Exposure time: 60s.")
![Western Blot: SYT11 Antibody - Azide and BSA Free [NBP2-93989] - Western blot analysis of extracts of various cell lines, using SYT11 antibody (A8198) at 1:600 dilution.<br>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.<br>Lysates/proteins: 25μg per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit (RM00020).<br>Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp2-93989_rabbit-polyclonal-syt11-antibody-5220251931366.jpg "Western Blot: SYT11 Antibody - Azide and BSA Free [NBP2-93989] - Western blot analysis of extracts of various cell lines, using SYT11 antibody (A8198) at 1:600 dilution.<br>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.<br>Lysates/proteins: 25μg per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit (RM00020).<br>Exposure time: 20s.")
![Immunocytochemistry/ Immunofluorescence: SYT11 Antibody - Azide and BSA Free [NBP2-93989] - Immunofluorescence analysis of mouse brain cells using SYT11 Rabbit pAb (A8198) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93989_rabbit-polyclonal-syt11-antibody-5220251931367.jpg)
![Immunocytochemistry/ Immunofluorescence: SYT11 Antibody - Azide and BSA Free [NBP2-93989] - Immunofluorescence analysis of rat brain cells using SYT11 Rabbit pAb (A8198) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93989_rabbit-polyclonal-syt11-antibody-5220251931333.jpg)
![Immunocytochemistry/ Immunofluorescence: SYT11 Antibody - Azide and BSA Free [NBP2-93989] - Immunofluorescence analysis of SH-5Y5Y cells using SYT11 Rabbit pAb (A8198) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93989_rabbit-polyclonal-syt11-antibody-5220251930292.jpg)
![Western Blot: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Analysis of extracts of various cell lines, using CHRM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Western-Blot-NBP2-94511-img0001.jpg "Western Blot: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Analysis of extracts of various cell lines, using CHRM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Analysis of SH-SY5Y cells using CHRM1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94511-img0004.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Mouse brain using CHRM1 Rabbit pAb at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-Paraffin-NBP2-94511-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Analysis of Neuro-2a cells using CHRM1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94511-img0003.jpg)
![Western Blot: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319] - analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S96-55 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/GABA-A-R-beta-1-Antibody-S96-55-Western-Blot-NBP1-48319-img0004.jpg "Western Blot: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319] - analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S96-55 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Positive.](http://images.novusbio.com/fullsize/GABA-A-R-beta-1-Antibody-S96-55-Immunocytochemistry-Immunofluorescence-NBP1-48319-img0003.jpg)
![Western Blot: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis using GRIA2 mAb against human GRIA2 (AA: 35-175) recombinant protein. (Expected MW is 41.9 kDa)](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Western-Blot-NBP2-61775-img0004.jpg "Western Blot: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis using GRIA2 mAb against human GRIA2 (AA: 35-175) recombinant protein. (Expected MW is 41.9 kDa)")
![Immunohistochemistry-Paraffin: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis of rectum cancer tissues using GRIA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Immunohistochemistry-NBP2-61775-img0003.jpg)
![Flow Cytometry: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis of SK-N-SH cells using GRIA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Flow-Cytometry-NBP2-61775-img0002.jpg)
![Western Blot: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis using GRIA2 mAb against HEK293 (1) and GRIA2 (AA: 35-175)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Western-Blot-NBP2-61775-img0005.jpg)
![ELISA: GluR2 Antibody (7A7A3) [NBP2-61775] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-ELISA-NBP2-61775-img0001.jpg)
![Western Blot: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700] - Analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 5 protein using Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody, Clone S415-24 . Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa.](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-S415-24-Western-Blot-NBP2-59700-img0002.jpg "Western Blot: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700] - Analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 5 protein using Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody, Clone S415-24 . Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa.")
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-AR alpha 5 Alpha 5 Monoclonal Antibody, Clone S415-24 (NBP2-59700). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-AR alpha 5 Alpha 5 Monoclonal Antibody (NBP2-59700) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-AR alpha 5 Alpha 5 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-S415-24-Immunocytochemistry-Immunofluorescence-NBP2-59700-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700] - Analysis using Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody, Clone S415-24 . Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABA-A Receptor Alpha5 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-S415-24-Immunocytochemistry-Immunofluorescence-NBP2-59700-img0001.jpg)
![Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Ser309]-Antibody-Western-Blot-NB300-236-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Synaptotagmin-1 [p Ser309] Antibody [NB300-236] - Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Ser309 in red and PSD95 in green. Photo courtesy of Gang Liu.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Ser309]-Antibody-Immunohistochemistry-NB300-236-img0002.jpg)
![Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against human NOS1 (AA: 17-153) recombinant protein. (Expected MW is 40.3 kDa)](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Western-Blot-NBP2-52520-img0003.jpg "Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against human NOS1 (AA: 17-153) recombinant protein. (Expected MW is 40.3 kDa)")
![Immunocytochemistry/Immunofluorescence: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis of HeLa cells using NOS1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Immunofluorescence-NBP2-52520-img0002.jpg)
![Flow Cytometry: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis of HeLa cells using NOS1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Flow-Cytometry-NBP2-52520-img0001.jpg)
![Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against HEK293 (1) and NOS1 (AA: 17-153)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Western-Blot-NBP2-52520-img0004.jpg)
![ELISA: nNOS Antibody (2E11G6) [NBP2-52520] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-ELISA-NBP2-52520-img0005.jpg)
![Western Blot: GluR7/GRIK3 Antibody (2B3D1) [NBP2-61782] - Analysis using GRIK3 mAb against human GRIK3 (AA: extra 32-173) recombinant protein. (Expected MW is 42 kDa)](http://images.novusbio.com/fullsize/GluR7-GRIK3-Antibody-2B3D1-Western-Blot-NBP2-61782-img0004.jpg "Western Blot: GluR7/GRIK3 Antibody (2B3D1) [NBP2-61782] - Analysis using GRIK3 mAb against human GRIK3 (AA: extra 32-173) recombinant protein. (Expected MW is 42 kDa)")
![Immunocytochemistry/Immunofluorescence: GluR7/GRIK3 Antibody (2B3D1) [NBP2-61782] - Analysis of Hela cells using GRIK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/GluR7-GRIK3-Antibody-2B3D1-Immunocytochemistry-Immunofluorescence-NBP2-61782-img0003.jpg)
![Western Blot: GluR7/GRIK3 Antibody (2B3D1) [NBP2-61782] - Analysis using GRIK3 mAb against HEK293 (1) and GRIK3 (AA: extra 32-173)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GluR7-GRIK3-Antibody-2B3D1-Western-Blot-NBP2-61782-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: GluR7/GRIK3 Antibody (2B3D1) [NBP2-61782] - Analysis of Hela cells using GRIK3 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/GluR7-GRIK3-Antibody-2B3D1-Immunocytochemistry-Immunofluorescence-NBP2-61782-img0002.jpg)
![ELISA: GluR7/GRIK3 Antibody (2B3D1) [NBP2-61782] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/GluR7-GRIK3-Antibody-2B3D1-ELISA-NBP2-61782-img0001.jpg)
![Western Blot: mGluR2 Antibody (6E4F6) [NBP2-61834] - Analysis using GRM2 mouse mAb against SK-N-SH (1), Jurkat (2), and SW620 (3) cell lysate.](http://images.novusbio.com/fullsize/mGluR2-Antibody-6E4F6-Western-Blot-NBP2-61834-img0004.jpg "Western Blot: mGluR2 Antibody (6E4F6) [NBP2-61834] - Analysis using GRM2 mouse mAb against SK-N-SH (1), Jurkat (2), and SW620 (3) cell lysate.")
![Western Blot: mGluR2 Antibody (6E4F6) [NBP2-61834] - Analysis using GRM2 mAb against human GRM2 (AA: extra 414-558) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/mGluR2-Antibody-6E4F6-Western-Blot-NBP2-61834-img0003.jpg)
![Western Blot: mGluR2 Antibody (6E4F6) [NBP2-61834] - Analysis using GRM2 mAb against HEK293 (1) and GRM2 (AA: extra 414-558)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/mGluR2-Antibody-6E4F6-Western-Blot-NBP2-61834-img0005.jpg)
![ELISA: mGluR2 Antibody (6E4F6) [NBP2-61834] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/mGluR2-Antibody-6E4F6-ELISA-NBP2-61834-img0001.jpg)
![ELISA: mGluR2 Antibody (6E4F6) [NBP2-61834] - Analysis of SK-N-SH cells using GRM2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/mGluR2-Antibody-6E4F6-ELISA-NBP2-61834-img0002.jpg)
![Western Blot: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody (NBP2-59326) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.](http://images.novusbio.com/fullsize/GABA-AR-alpha-4-Antibody-S398A-34-Western-Blot-NBP2-59326-img0007.jpg "Western Blot: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody (NBP2-59326) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.")
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody (NBP2-59326) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha 4 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GABA-AR-alpha-4-Antibody-S398A-34-Immunocytochemistry-Immunofluorescence-NBP2-59326-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Analysis using Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody, Clone S398A-34. (Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT.](http://images.novusbio.com/fullsize/GABA-AR-alpha-4-Antibody-S398A-34-Immunocytochemistry-Immunofluorescence-NBP2-59326-img0004.jpg)