| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50537-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99063-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45348 | |||
| Azide and BSA Free | NBP2-34744-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59628 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-120SS | |||
![Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Ser309]-Antibody-Western-Blot-NB300-236-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Synaptotagmin-1 [p Ser309] Antibody [NB300-236] - Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Ser309 in red and PSD95 in green. Photo courtesy of Gang Liu.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Ser309]-Antibody-Immunohistochemistry-NB300-236-img0002.jpg)
![Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Western-Blot-NBP2-99063-img0003.jpg "Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa")
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-99063] - Immunochemical staining of human Calbindin D-28K in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-99063-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-99063] - Immunofluorescence staining of Calbindin D-28K in 293 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-99063 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-99063_rabbit-polyclonal-calbindin-d-28k-antibody-235202514331592.png)
![Immunoprecipitation: Calbindin D-28K Antibody [NBP2-99063] - Calbindin D-28K was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 4 ul NBP2-99063 and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: NBP2-99063, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa](http://images.novusbio.com/fullsize/nbp2-99063_rabbit-polyclonal-calbindin-d-28k-antibody-235202516335024.png)
![Immunohistochemistry: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/20,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32360) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32360_rabbit-gaba-b-r1-mab-psh03-87-298202414304191.jpg "Immunohistochemistry: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/20,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32360) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABA-B R1 with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/50 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32360, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32360_rabbit-gaba-b-r1-mab-psh03-87-298202413585541.jpg)
![Western Blot: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Western blot analysis of GABA-B R1 on different lysates with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/2,000 dilution.<br/> Lane 1: Human brain tissue lysate<br/> Lane 2: Mouse brain tissue lysate <br/> Lane 3: Rat brain tissue lysate (no heat)<br/> Lane 4: Mouse cerebellum tissue lysate (70℃ heat)<br/> Lane 5: Rat cerebellum tissue lysate<br/> Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative)<br/> Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative)<br/><br/> Lysates/proteins at 40 µg/Lane.<br/><br/> Predicted band size: 108 kDa<br/> Observed band size: 95-108 kDa<br/><br/> Exposure time: <br/> Lane 1-7 (left): 28 seconds; <br/> Lane 1-5 (right): 2 minutes 34 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32360_rabbit-gaba-b-r1-mab-psh03-87-3920248453530.jpg)
![Western blot analysis on different lysates with NBP3-32360 at 1/5,000 dilution. Lane 1: Human brain tissue lysate (40 µg/Lane) Lane 2: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 3: Rat brain tissue lysate (no heat) (20 µg/Lane) Lane 4: Mouse cerebellum tissue lysate (70℃ heat) (20 µg/Lane) Lane 5: Rat cerebellum tissue lysate (20 µg/Lane) Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane) Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 108 kDa Observed band size: 95-108 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32360 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32360_-western-blot-639204597483663345.jpg)
![Immunocytochemistry/Immunofluorescence: mGluR3 Antibody [NLS3218] - Analysis of anti-GRM3 / MGLUR3 antibody with transfected HEK293 human embryonic kidney cells.](http://images.novusbio.com/fullsize/mGluR3-Antibody-Immunocytochemistry-Immunofluorescence-NLS3218-img0010.jpg "Immunocytochemistry/Immunofluorescence: mGluR3 Antibody [NLS3218] - Analysis of anti-GRM3 / MGLUR3 antibody with transfected HEK293 human embryonic kidney cells.")
![Immunohistochemistry-Paraffin: mGluR3 Antibody [NLS3218] - Anti-GRM3 / MGLUR3 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/mGluR3-Antibody-Immunohistochemistry-Paraffin-NLS3218-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: mGluR3 Antibody [NLS3218] - Analysis of anti-GRM3 / MGLUR3 antibody with untransfected HEK293 human embryonic kidney cells.](http://images.novusbio.com/fullsize/mGluR3-Antibody-Immunocytochemistry-Immunofluorescence-NLS3218-img0009.jpg)
![Immunohistochemistry-Paraffin: mGluR3 Antibody [NLS3218] - Analysis of anti-GRM3 / MGLUR3 antibody with human brain, neurons and glia at 9 ug/ml](http://images.novusbio.com/fullsize/mGluR3-Antibody-Immunohistochemistry-Paraffin-NLS3218-img0012.jpg)
![Immunohistochemistry: NPY5R Antibody [NBP3-14426] - Immunohistochemistry of breast carcinoma](http://images.novusbio.com/fullsize/NPY5R-Antibody-Immunohistochemistry-NBP3-14426-img0005.jpg "Immunohistochemistry: NPY5R Antibody [NBP3-14426] - Immunohistochemistry of breast carcinoma")
![Immunohistochemistry-Paraffin: NPY5R Antibody [NBP3-14426] - Immunohistochemistry of formalin-fixed, paraffin-embedded human brain, glioblastoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/NPY5R-Antibody-Immunohistochemistry-Paraffin-NBP3-14426-img0001.jpg)
![Immunohistochemistry-Paraffin: NPY5R Antibody [NBP3-14426] - Immunohistochemistry of formalin-fixed, paraffin-embedded human lung, adenocarcinoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/NPY5R-Antibody-Immunohistochemistry-Paraffin-NBP3-14426-img0002.jpg)
![Immunohistochemistry-Paraffin: NPY5R Antibody [NBP3-14426] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skin melanoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/NPY5R-Antibody-Immunohistochemistry-Paraffin-NBP3-14426-img0003.jpg)
![Immunohistochemistry-Paraffin: NPY5R Antibody [NBP3-14426] - Immunohistochemistry of formalin-fixed, paraffin-embedded human brain, neurons and glia, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/NPY5R-Antibody-Immunohistochemistry-Paraffin-NBP3-14426-img0004.jpg)
![Immunohistochemistry: NPY5R Antibody [NBP3-14426] - Immunohistochemistry of colon carcinoma](http://images.novusbio.com/fullsize/NPY5R Antibody-Immunohistochemistry-NBP3-14426-img0006.jpg)
![Immunohistochemistry: Somatostatin R1/SSTR1 Antibody [NBP3-14480] - Immunohistochemistry of ovary carcinoma](http://images.novusbio.com/fullsize/Somatostatin-R1-SSTR1-Antibody-Immunohistochemistry-NBP3-14480-img0006.jpg "Immunohistochemistry: Somatostatin R1/SSTR1 Antibody [NBP3-14480] - Immunohistochemistry of ovary carcinoma")
![Immunohistochemistry-Paraffin: Somatostatin R1/SSTR1 Antibody [NBP3-14480] - Immunohistochemistry of formalin-fixed, paraffin-embedded human pancreas, islet of Langerhans, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Somatostatin-R1-SSTR1-Antibody-Immunohistochemistry-Paraffin-NBP3-14480-img0001.jpg)
![Immunohistochemistry-Paraffin: Somatostatin R1/SSTR1 Antibody [NBP3-14480] - Immunohistochemistry of formalin-fixed, paraffin-embedded human lung, small cell carcinoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Somatostatin-R1-SSTR1-Antibody-Immunohistochemistry-Paraffin-NBP3-14480-img0002.jpg)
![Immunohistochemistry-Paraffin: Somatostatin R1/SSTR1 Antibody [NBP3-14480] - Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate carcinoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Somatostatin-R1-SSTR1-Antibody-Immunohistochemistry-Paraffin-NBP3-14480-img0003.jpg)
![Immunohistochemistry-Paraffin: Somatostatin R1/SSTR1 Antibody [NBP3-14480] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skin melanoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Somatostatin-R1-SSTR1-Antibody-Immunohistochemistry-Paraffin-NBP3-14480-img0004.jpg)
![Immunohistochemistry: Somatostatin R1/SSTR1 Antibody [NBP3-14480] - Immunohistochemistry of breast carcinoma](http://images.novusbio.com/fullsize/Somatostatin-R1-SSTR1-Antibody-Immunohistochemistry-NBP3-14480-img0005.jpg)
![Immunohistochemistry: Somatostatin R1/SSTR1 Antibody [NBP3-14480] - Immunohistochemistry of pancreas carcinoma](http://images.novusbio.com/fullsize/Somatostatin R1-SSTR1 Antibody-Immunohistochemistry-NBP3-14480-img0007.jpg)
![Western Blot: eNOS Antibody (M221) [NBP3-23209] - Western blot analysis of human umbilical vein endothelial cells before (lanes 1, 3, 5) and after (lanes 2, 4, 6) treatment with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23209_mouse-enos-mab-m221-19920231856919.jpg "<b>Biological Strategies Validation. </b>Western Blot: eNOS Antibody (M221) [NBP3-23209] - Western blot analysis of human umbilical vein endothelial cells before (lanes 1, 3, 5) and after (lanes 2, 4, 6) treatment with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: eNOS Antibody (M221) [NBP3-23209] - Immunocytochemical labeling of endothelial nitric oxide synthase (eNOS) in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with mouse monoclonal eNOS (NBP3-23209) and rabbit polyclonal eNOS (NBP3-23210) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23209_mouse-enos-mab-m221-11920231633165.jpg)
![Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (SPM166) [NBP2-32919] - Formalin-fixed, paraffin-embedded mouse intestine tissue stained with BrdU MAb (SPM166).](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-SPM166-Immunohistochemistry-Paraffin-NBP2-32919-img0001.jpg "Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (SPM166) [NBP2-32919] - Formalin-fixed, paraffin-embedded mouse intestine tissue stained with BrdU MAb (SPM166).")
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Knockout-Validated-NBP2-59626-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Protein Array: p53 Antibody (TRP/816) [NBP2-59626] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Protein-Array-NBP2-59626-img0001.jpg)
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Western-Blot-NBP2-59626-img0006.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/816) [NBP2-59626] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p53 Mouse Monoclonal Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Immunohistochemistry-Paraffin-NBP2-59626-img0002.jpg)
![Western Blot: Somatostatin R1/SSTR1 Antibody [NBP2-27413] - Analysis in human A) lung and B) pancreas lysate using SSTR1 antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/Somatostatin-R1-SSTR1-Antibody-Western-Blot-NBP2-27413-img0002.jpg "Western Blot: Somatostatin R1/SSTR1 Antibody [NBP2-27413] - Analysis in human A) lung and B) pancreas lysate using SSTR1 antibody at 3 ug/ml.")
![Western Blot: Somatostatin Receptor 1 Antibody [NB300-120] - Detection of SSR1 from rat pancreas using NB300-120 (1:1000).](http://images.novusbio.com/fullsize/Somatostatin-Receptor-1-Antibody-Western-Blot-NB300-120-img0003.jpg "Western Blot: Somatostatin Receptor 1 Antibody [NB300-120] - Detection of SSR1 from rat pancreas using NB300-120 (1:1000).")