| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61843-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97471-0.2mg | |||
| BSA Free | NBP1-97471-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-41535SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78403SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB2416-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24781SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-62009B | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25953 | |||
![Western Blot: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis using GRM3 mAb against human GRM3 (AA: extra 433-576) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Western-Blot-NBP2-61843-img0005.jpg "Western Blot: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis using GRM3 mAb against human GRM3 (AA: extra 433-576) recombinant protein. (Expected MW is 42.4 kDa)")
![Immunocytochemistry/Immunofluorescence: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis of Hela cells using GRM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Immunocytochemistry-Immunofluorescence-NBP2-61843-img0003.jpg)
![Immunohistochemistry-Paraffin: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis of cervical cancer tissues using GRM3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Immunohistochemistry-NBP2-61843-img0004.jpg)
![Flow Cytometry: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis of SH-SY5Y cells using GRM3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-ELISA-NBP2-61843-img0002.jpg)
![Western Blot: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis using GRM3 mAb against HEK293 (1) and GRM3 (AA: extra 433-576)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Western-Blot-NBP2-61843-img0006.jpg)
![ELISA: mGluR3 Antibody (7A5A6) [NBP2-61843] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-ELISA-NBP2-61843-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Western Blot: iNOS Antibody [NBP1-97471] - Rat brain extract, probed with iNOS, pAb.](http://images.novusbio.com/fullsize/iNOS-Antibody-Western-Blot-NBP1-97471-img0001.jpg "Western Blot: iNOS Antibody [NBP1-97471] - Rat brain extract, probed with iNOS, pAb.")
![Immunohistochemistry: NK1R Antibody (JE39-75) [NBP3-32653] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-NK1R antibody (NBP3-32653) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32653) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32653_rabbit-nk1r-mab-je39-75-298202413585593.jpg "Immunohistochemistry: NK1R Antibody (JE39-75) [NBP3-32653] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-NK1R antibody (NBP3-32653) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32653) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: NK1R Antibody (JE39-75) [NBP3-32653] - Immunocytochemistry analysis of Neuro-2a cells labeling NK1R with Rabbit anti-NK1R antibody (NBP3-32653) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NK1R antibody (NBP3-32653) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32653_rabbit-nk1r-mab-je39-75-29202410234275.jpg)
![Flow Cytometry: NK1R Antibody (JE39-75) [NBP3-32653] - Flow cytometric analysis of Neuro-2a cells labeling NK1R.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32653, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32653_rabbit-nk1r-mab-je39-75-29202416453284.jpg)
![Western Blot: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Analysis of GABA A Receptor alpha 5 on different lysates using anti-GABA A Receptor alpha 5 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: Rat brain tissue lysate](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-JB34-19-Western-Blot-NBP2-75497-img0006.jpg "Western Blot: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Analysis of GABA A Receptor alpha 5 on different lysates using anti-GABA A Receptor alpha 5 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: Rat brain tissue lysate")
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Staining GABA A Receptor alpha 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-JB34-19-Immunocytochemistry-Immunofluorescence-NBP2-75497-img0003.jpg)
![Immunohistochemistry-Paraffin: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Analysis of paraffin-embedded mouse brain tissue using anti-GABA A Receptor alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-JB34-19-Immunohistochemistry-Paraffin-NBP2-75497-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Staining GABA A Receptor alpha 5 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-JB34-19-Immunocytochemistry-Immunofluorescence-NBP2-75497-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Staining GABA A Receptor alpha 5 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-JB34-19-Immunocytochemistry-Immunofluorescence-NBP2-75497-img0002.jpg)
![Immunohistochemistry-Paraffin: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Analysis of paraffin-embedded rat brain tissue using anti-GABA A Receptor alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-JB34-19-Immunohistochemistry-Paraffin-NBP2-75497-img0005.jpg)
![Western Blot: TREK 1 Antibody [NB110-41535] - Detection of TREK 1 in membrane lysate fraction of human brain tissue.](http://images.novusbio.com/fullsize/TREK-1-Antibody-Western-Blot-NB110-41535-img0003.jpg "Western Blot: TREK 1 Antibody [NB110-41535] - Detection of TREK 1 in membrane lysate fraction of human brain tissue.")
![Western Blot: 5-HT4 Antibody [NBP1-78403] - Western Blot Image of anti-5HT4 Receptor. Total protein from human liver tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LYVE1 in 1% milk/TBST. Reactivity was detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/5-HT4-Antibody-Western-Blot-NBP1-78403-img0003.jpg "Western Blot: 5-HT4 Antibody [NBP1-78403] - Western Blot Image of anti-5HT4 Receptor. Total protein from human liver tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LYVE1 in 1% milk/TBST. Reactivity was detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: 5-HT4 Antibody [NBP1-78403] - Staining of 5HT4 receptor in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/5-HT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78403-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15334-img0001.jpg "Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - Cal27 xenograft, using ALDH1A1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP2-15334-img0002.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Western-Blot-NBP2-15334-img0003.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - A. 50 ug mouse liver lysate/extract 10 % SDS-PAGE ALDH1A1 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NBP2-15334-img0006.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - PG-4 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP2-15334) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15334_rabbit-polyclonal-aldehyde-dehydrogenase-1-a1-aldh1a1-antibody-9102024206330.jpg)
![Western Blot: nNOS Antibody (60718) [MAB2416] - Analysis of mouse and rat brain Tissue lysate with ug/ml of hnNOS Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hnNOS at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/nNOS-Antibody-60718-Western-Blot-MAB2416-img0001.jpg "Western Blot: nNOS Antibody (60718) [MAB2416] - Analysis of mouse and rat brain Tissue lysate with ug/ml of hnNOS Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hnNOS at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Western Blot: PGP9.5 / UCHL-1 Antibody [NBP2-24781] - Western blot analysis of PGP9.5 / UCHL-1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using PGP9.5 / UCHL-1 antibody at 0.2 ug/ml.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NBP2-24781-img0005.jpg "Western Blot: PGP9.5 / UCHL-1 Antibody [NBP2-24781] - Western blot analysis of PGP9.5 / UCHL-1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using PGP9.5 / UCHL-1 antibody at 0.2 ug/ml.")
![Immunohistochemistry-Paraffin: PGP9.5 / UCHL-1 Antibody [NBP2-24781] - IHC analysis of human brain, cortex, using PGP9.5 / UCHL-1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunohistochemistry-Paraffin-NBP2-24781-img0003.jpg)
![Western Blot: IgG2b Antibody (RM108) [Unconjugated] [NBP2-62009] - Western blot of nonreduced(-) and reduced(+) mouse IgG2b, using NBP2-62009. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced g2b form (~50 kDa).](http://images.novusbio.com/fullsize/IgG2b-Antibody-RM108-Unconjugated-Western-Blot-NBP2-62009-img0004.jpg "Western Blot: IgG2b Antibody (RM108) [Unconjugated] [NBP2-62009] - Western blot of nonreduced(-) and reduced(+) mouse IgG2b, using NBP2-62009. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced g2b form (~50 kDa).")
![ELISA: IgG2b Antibody (RM108) - Azide and BSA Free [NBP2-62009] - A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62009_rabbit-monoclonal-igg2b-antibody-rm108-elisa-189202523423523.jpg)
![ELISA: IgG2b Antibody (RM108) - Azide and BSA Free [NBP2-62009] - ELISA of mouse immunoglobulins shows RM108 reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62009_rabbit-monoclonal-igg2b-antibody-rm108-elisa-189202523403523.jpg)