| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47440-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79854 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41246-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47441-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77317-0.025mg | |||
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Western-Blot-NBP2-17247-img0027.jpg "<b>Biological Strategies Validation. </b>Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer. MDM2 stained by MDM2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0028.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer, using MDM2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0003.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. (a) Western blot analysis of p53 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (b,c) Western blot analysis of p53 in Hif-p4h-1 & scrambled (Scrm) siRNA transfected HEK293 cells (b) & wt MEFs treated with 50 µM FG4497 for 24 h (c). (d) qPCR analysis of p53 mRNA in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (e) Analysis of p53 protein turnover rate. Hif-p4h-1−/− & wt MEFs were treated with 200 μg/ml of cycloheximide for the indicated time points & whole cell lysates were blotted for p53. (f) Western blot analysis of MDM2 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (g) Western blot analysis of p53 & MDM2 in wt MEFs treated with 10 μM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). (h,i) Western blot analysis of HIF1 alpha in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h (h) & in scrambled & Hif-p4h-1 siRNA transfected HEK293 cells (i). (j) Western blot analysis of ubiquitination of p53 in Hif-p4h-1 & scrambled siRNA transfected HEK293 cells. The cells were transfected with HA-ubiquitin & endogenous p53 was immunoprecipitated followed by Western blotting with anti-HA & anti-P53 antibodies. (k) Western blot analysis of p53 in Hif-p4h-1−/− & wt MEFs were treated with or without 10 μM MG132 for 5 h. Data are presented as representative Western blots & as mean ± s.d., n = at least 3 individual MEF isolates or experiments. *P Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091472.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242010445.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091496.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - MDM2/HDM2 antibody detects MDM2/HDM2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-910202420134015.jpg)
![MDM2 antibody immunoprecipitates MDM2 protein in IP experiments.IP samples: Jurkat whole cell extractA. 30 ug Jurkat cell whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of MDM2 protein by 4 ug MDM2 antibody (NBP2-17247)7.5 % SDS-PAGEThe immunoprecipitated MDM2 protein was detected by MDM2 antibody (NBP2-17247) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-immunoprecipitation-261120251826130.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPSA antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0018.jpg "Western Blot: RPSA Antibody [NBP1-33002] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPSA antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: RPSA Antibody [NBP1-33002] - CA922 xenograft, using RPSA antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RPSA-Antibody-Immunohistochemistry-Paraffin-NBP1-33002-img0012.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0013.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Whole cell extracts (30 ug) was separated by 10 % SDS-PAGE, and blotted with RPSA antibody diluted by 1:1000](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0014.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Liver protein expression in different experimental groups of Laminin R, AdipoR2 and SIRT1.Western Blotting analysis of protein expression in the liver on different experimental groups of aminin Receptor. Image shows demonstrative bands of the analyzed proteins and respective housekeeping protein (beta-tubulin) in liver. Data are expressed in mean +/- s.e.m. *p<0.05 Control diet and EGCG (CE) group versus Control diet and Water (CW) group. #p<0.05 High-fat diet and EGCG (HFE) group versus HFW group. $p<0.05 HFW versus CW. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0141227), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0015.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Sample (30 ug of whole cell lysate). A: Jurkat. 12% SDS PAGE. RPSA Antibody diluted at 1:1000](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0016.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - RPSA antibody detects RPSA protein by Western blot analysis. Mouse tissue extracts (50 ug) was separated by 10 % SDS-PAGE, and the membrane was blotted with RPSA antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0017.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody [NBP1-33002] - Mouse small intestine. RPSA antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RPSA-Antibody-Immunohistochemistry-Paraffin-NBP1-33002-img0009.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - RPSA antibody detects RPSA protein by Western blot analysis. Whole cell extracts (30 ug) was separated by 10 % SDS-PAGE, and blotted with RPSA antibody (NBP1-33002) diluted by 1:1000](http://images.novusbio.com/fullsize/nbp1-33002_rabbit-polyclonal-rpsa-antibody-9102024205675.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining in human testis and liver tissues using anti-AKAP8L antibody. Corresponding AKAP8L RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47440-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining in human testis and liver tissues using anti-AKAP8L antibody. Corresponding AKAP8L RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining of human cerebral cortex, colon, kidney and testis using Anti-AKAP8L antibody NBP2-47440 (A) shows similar protein distribution across tissues to independent antibody NBP2-47441 (B).](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47440-img0008.jpg)
![Western Blot: HA95/AKAP8L Antibody [NBP2-47440] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Western-Blot-NBP2-47440-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HA95/AKAP8L Antibody [NBP2-47440] - Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47440-img0003.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47440-img0010.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47440-img0004.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47440-img0005.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining of human colon.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47440-img0009.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining of human kidney.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47440-img0011.jpg)
![Western Blot: RPSA Antibody (RPSA/2699) [NBP2-79720] - Western Blot Analysis of human THP-1, U937, SK-BR3, and A431 cell lysates. RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Western-Blot-NBP2-79720-img0010.jpg "Western Blot: RPSA Antibody (RPSA/2699) [NBP2-79720] - Western Blot Analysis of human THP-1, U937, SK-BR3, and A431 cell lysates. RPSA Antibody (RPSA/2699).")
![Immunocytochemistry/Immunofluorescence: RPSA Antibody (RPSA/2699) [NBP2-79720] - Immunofluorescent staining of paraformaldehyde-Raji cells using RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunocytochemistry-Immunofluorescence-NBP2-79720-img0003.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0008.jpg)
![Flow Cytometry: RPSA Antibody (RPSA/2699) [NBP2-79720] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Flow-Cytometry-NBP2-79720-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: RPSA Antibody (RPSA/2699) [NBP2-79720] - Immunofluorescent staining of HeLa cells using RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunocytochemistry-Immunofluorescence-NBP2-79720-img0002.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0004.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0005.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Cervix Carcinoma stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0006.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Tonsil stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0007.jpg)
![Protein Array: RPSA Antibody (RPSA/2699) [NBP2-79720] - Analysis of Protein Array containing more than 19,000 full-length human proteins using RPSA Antibody (RPSA/2699) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Protein-Array-NBP2-79720-img0009.jpg)
![Western Blot: RPSA Antibody [NBP2-41246] - Analysis of RPSA in Caco-2 cell lysate with RPSA antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP2-41246-img0004.jpg "Western Blot: RPSA Antibody [NBP2-41246] - Analysis of RPSA in Caco-2 cell lysate with RPSA antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: RPSA Antibody - BSA Free [NBP2-41246] - Immunofluorescence of RPSA in mouse liver tissue with RPSA antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41246_rabbit-polyclonal-rpsa-antibody-2711202420195344.jpg)
![Immunohistochemistry: RPSA Antibody - BSA Free [NBP2-41246] - Immunohistochemistry of RPSA in mouse brain tissue with RPSA Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41246_rabbit-polyclonal-rpsa-antibody-271120242045639.gif)
![Immunohistochemistry: RPSA Antibody - BSA Free [NBP2-41246] - Immunohistochemistry of RPSA in mouse liver tissue with RPSA antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41246_rabbit-polyclonal-rpsa-antibody-271120242004162.jpg)
![Immunocytochemistry/ Immunofluorescence: RPSA Antibody - BSA Free [NBP2-41246] - Immunofluorescence of RPSA in mouse brain tissue with RPSA Antibody at 20 ug/mL.Green: RPSA antibody Red: Phylloidin staining Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-41246_rabbit-polyclonal-rpsa-antibody-271120242042517.gif)
![Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Western blot analysis of extracts of various cell lines, using CaM Kinase II Rabbit mAb (NBP3-15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Western-Blot-NBP3-15256-img0004.jpg "Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Western blot analysis of extracts of various cell lines, using CaM Kinase II Rabbit mAb (NBP3-15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry of paraffin-embedded mouse spinal cord using CaM Kinase II Rabbit mAb (NBP3-15256) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Immunohistochemistry-Paraffin-NBP3-15256-img0003.jpg)
![Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Western blot analysis of extracts of various cell lines, using CaM Kinase II Rabbit mAb (NBP3-15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Western-Blot-NBP3-15256-img0005.jpg)
![Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry of paraffin-embedded rat brain using CaM Kinase II Rabbit mAb (NBP3-15256) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Immunohistochemistry-Paraffin-NBP3-15256-img0001.jpg)
![Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry of paraffin-embedded human brain using CaM Kinase II Rabbit mAb (NBP3-15256) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Immunohistochemistry-Paraffin-NBP3-15256-img0002.jpg)
![Immunohistochemistry: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CaM Kinase II Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15256_rabbit-cam-kinase-ii-mab-9p2e2-72202512443379.jpg)
![Immunocytochemistry/Immunofluorescence: RPSA Antibody (RP-01) [NBP3-09109] - Staining of RPSA in human glioblastoma cells T98G using mouse monoclonal antibody RP-01. The cells were fixed with paraformaldehyde and permeabilized using Triton X-100.](http://images.novusbio.com/fullsize/RPSA-Antibody-RP-01-Immunocytochemistry-Immunofluorescence-NBP3-09109-img0001.jpg "Immunocytochemistry/Immunofluorescence: RPSA Antibody (RP-01) [NBP3-09109] - Staining of RPSA in human glioblastoma cells T98G using mouse monoclonal antibody RP-01. The cells were fixed with paraformaldehyde and permeabilized using Triton X-100.")
![Western Blot: Mouse Monoclonal RPSA Antibody (RP-01) [NBP3-09109] - Western blotting analysis was performed on urea buffer extracts of Ramos, Raji, HeLa, Jurkat, and Huvec cells mixed with hot reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.Nitrocellulose membrane blot was probed with mouse IgG2a monoclonal antibody RP-01 (2 ug/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.RPSA was detected at ~37 kDa in all tested cell lines.](http://images.novusbio.com/fullsize/antibody/nbp3-09109_mouse-monoclonal-rpsa-antibody-rp-01-sandwich-elisa-151202513658..jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47441] - Staining of human colon.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47441-img0009.jpg "Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47441] - Staining of human colon.")
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47441] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47441-img0003.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47441] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47441-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47441] - Staining in human testis and liver tissues using anti-AKAP8L antibody. Corresponding AKAP8L RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47441-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47441] - Staining of human cerebral cortex, colon, kidney and testis using Anti-AKAP8L antibody NBP2-47441 (A) shows similar protein distribution across tissues to independent antibody NBP2-47440 (B).](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47441-img0006.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47441] - Staining of human kidney.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47441-img0007.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47441] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47441-img0008.jpg)
![Western Blot: RPSA Antibody (MLuC5) [NB200-631] - Analysis of 25 ug of HepG2 (lane 1) and Hela (lane 2) cell lysates.](http://images.novusbio.com/fullsize/RPSA-Antibody-MLuC5-Western-Blot-NB200-631-img0005.jpg "Western Blot: RPSA Antibody (MLuC5) [NB200-631] - Analysis of 25 ug of HepG2 (lane 1) and Hela (lane 2) cell lysates.")
![Immunocytochemistry/Immunofluorescence: RPSA Antibody (MLuC5) [NB200-631] - Analysis of Laminin Receptor (green) showing staining in the membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RPSA-Antibody-MLuC5-Immunocytochemistry-Immunofluorescence-NB200-631-img0004.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (MLuC5) [NB200-631] - Human breast carcinoma stained with Laminin Receptor antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane with some cytoplasmic staining of tumor cells.](http://images.novusbio.com/fullsize/RPSA-Antibody-MLuC5-Immunohistochemistry-Paraffin-NB200-631-img0002.jpg)
![Flow Cytometry: RPSA Antibody (MLuC5) [NB200-631] - Analysis of Laminin Receptor in MCF-7 cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/RPSA-Antibody-MLuC5-Flow-Cytometry-NB200-631-img0003.jpg)
![Western Blot: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Analysis of MDM2 expression in transfected 293T cell line by MDM2 monoclonal antibody (M01), clone 1A7.Lane 1: MDM2 transfected lysate(55.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-1A7-Western-Blot-H00004193-M01-img0018.jpg "Western Blot: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Analysis of MDM2 expression in transfected 293T cell line by MDM2 monoclonal antibody (M01), clone 1A7.Lane 1: MDM2 transfected lysate(55.2 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Analysis of monoclonal antibody to MDM2 on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-1A7-Immunohistochemistry-Paraffin-H00004193-M01-img0015.jpg)
![Western Blot: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Analysis of MDM2 over-expressed 293 cell line, cotransfected with MDM2 Validated Chimera RNAi ( Cat # H00004193-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MDM2 monoclonal antibody (M01), clone 1A7 (Cat # H00004193-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-1A7-Western-Blot-H00004193-M01-img0016.jpg)
![Proximity Ligation Assay: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Analysis of protein-protein interactions between TP53 and MDM2. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-MDM2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-1A7-Proximity-Ligation-Assay-H00004193-M01-img0013.jpg)
![ELISA: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Detection limit for recombinant GST tagged MDM2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-1A7-ELISA-H00004193-M01-img0019.jpg)
![Western Blot: FBXL20 Antibody [NBP1-77317] - A20 cell lysate with FBXL20 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/FBXL20-Antibody-Western-Blot-NBP1-77317-img0001.jpg "Western Blot: FBXL20 Antibody [NBP1-77317] - A20 cell lysate with FBXL20 antibody at (A) 0.5 and (B) 1 ug/mL.")
![Immunohistochemistry-Paraffin: FBXL20 Antibody [NBP1-77317] - Human brain tissue using FBXL20 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/FBXL20-Antibody-Immunohistochemistry-Paraffin-NBP1-77317-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: FBXL20 Antibody - BSA Free [NBP1-77317] - Immunofluorescence of scrapper in human brain tissue with scrapper antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77317_rabbit-polyclonal-fbxl20-antibody-2711202419592058.jpg)
![Western Blot: RPSA Antibody (2Q8Q6) [NBP3-16624] - Western blot analysis of extracts of various cell lines, using RPSA Rabbit mAb (NBP3-16624) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/RPSA-Antibody-2Q8Q6-Western-Blot-NBP3-16624-img0002.jpg "Western Blot: RPSA Antibody (2Q8Q6) [NBP3-16624] - Western blot analysis of extracts of various cell lines, using RPSA Rabbit mAb (NBP3-16624) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: RPSA Antibody (2Q8Q6) [NBP3-16624] - Immunofluorescence analysis of NIH-3T3 cells using RPSA Rabbit mAb (NBP3-16624) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPSA-Antibody-2Q8Q6-Immunocytochemistry-Immunofluorescence-NBP3-16624-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: RPSA Antibody (2Q8Q6) [NBP3-16624] - Confocal imaging of NIH/3T3 cells using RPSA Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16624_rabbit-rpsa-mab-2q8q6-72202512435329.jpg)
![Immunocytochemistry/ Immunofluorescence: RPSA Antibody (2Q8Q6) [NBP3-16624] - Confocal imaging of HeLa cells using RPSA Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16624_rabbit-rpsa-mab-2q8q6-72202512435355.jpg)