| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12895-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB120-10505-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81016 | |||
| BSA Free | NB100-406SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91920-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43811 | |||
| BSA Free | NBP2-46627-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43815 | |||
| BSA Free | NBP2-46629-25ul | |||
![Western Blot: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Analysis of extracts of various cell lines, using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-7L7N2-Western-Blot-NBP3-16582-img0005.jpg "Western Blot: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Analysis of extracts of various cell lines, using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunofluorescence analysis of NIH-3T3 cells using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-7L7N2-Immunocytochemistry-Immunofluorescence-NBP3-16582-img0004.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Analysis of extracts of various cell lines, using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-7L7N2-Western-Blot-NBP3-16582-img0006.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511383740.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-522025125432.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511361270.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511411443.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-5220251215457.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511361223.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511411490.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511365186.jpg)
![Western Blot: HCN2 Antibody (S71-37) [NBP2-12895] - Western Blot analysis of Mouse Brain showing detection of ~95 kDa HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Lane 1: MW Ladder. Lane 2: Mouse Brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~95 kDa.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Western-Blot-NBP2-12895-img0010.jpg "Western Blot: HCN2 Antibody (S71-37) [NBP2-12895] - Western Blot analysis of Mouse Brain showing detection of ~95 kDa HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Lane 1: MW Ladder. Lane 2: Mouse Brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~95 kDa.")
![Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunocytochemistry-Immunofluorescence-NBP2-12895-img0013.jpg)
![Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895] - Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunohistochemistry-NBP2-12895-img0012.jpg)
![Western Blot: HCN2 Antibody (S71-37) [NBP2-12895] - Western Blot analysis of Rat brain membrane lysate showing detection of HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Western-Blot-NBP2-12895-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Human hippocampal tissues. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunocytochemistry-Immunofluorescence-NBP2-12895-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 (NBP2-12895). Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:100. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) HCN2 Antibody. (D) Hoechst (E) Composite.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunocytochemistry-Immunofluorescence-NBP2-12895-img0014.jpg)
![Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895] - Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunohistochemistry-NBP2-12895-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: BDNF Antibody (35928.11) [NB120-10505] - Human thyroid tissue was incubated with anti-BDNF antibody at a dilution of 1:100 overnight at 4C and detected with Alexa Fluor 594 Donkey anti-mouse 1:500. Nuclei stained with DAPI. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/BDNF-Antibody-35928-11-Immunocytochemistry-Immunofluorescence-NB120-10505-img0001.jpg "Immunocytochemistry/Immunofluorescence: BDNF Antibody (35928.11) [NB120-10505] - Human thyroid tissue was incubated with anti-BDNF antibody at a dilution of 1:100 overnight at 4C and detected with Alexa Fluor 594 Donkey anti-mouse 1:500. Nuclei stained with DAPI. ICC/IF image submitted by a verified customer review.")
![Western Blot: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Analysis of NMDAR1 on different cells lysates using anti-NMDAR1 antibody at 1/500 dilution. Positive control: Line 1: MCF-7 Line 2: A549](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Western-Blot-NBP2-66982-img0004.jpg "Western Blot: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Analysis of NMDAR1 on different cells lysates using anti-NMDAR1 antibody at 1/500 dilution. Positive control: Line 1: MCF-7 Line 2: A549")
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Staining NMDAR1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Immunocytochemistry-Immunofluorescence-NBP2-66982-img0005.jpg)
![Immunohistochemistry-Paraffin: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Analysis of paraffin-embedded rat brain tissue using anti-NMDAR1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Immunohistochemistry-Paraffin-NBP2-66982-img0003.jpg)
![Flow Cytometry: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Analysis of SH-SY5Y cells with NMDAR1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Flow-Cytometry-NBP2-66982-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Staining NMDAR1 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Immunocytochemistry-Immunofluorescence-NBP2-66982-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Staining NMDAR1 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Immunocytochemistry-Immunofluorescence-NBP2-66982-img0006.jpg)
![Immunohistochemistry-Paraffin: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Analysis of paraffin-embedded mouse brain tissue using anti-NMDAR1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Immunohistochemistry-Paraffin-NBP2-66982-img0002.jpg)
![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Western-Blot-NBP2-54787-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate")
![Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde made from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54787-img0006.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to when 100 uM of active alpha Synuclein monomer (NBP2-54788) is combined with 10 nM of control alpha Synuclein aggregate (NBP2-54787), or 100 uM of control alpha Synuclein monomer (NBP2-54786) is combined with 10 nM of control alpha Synuclein aggregate (NBP2-54787). Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-In-vitro-assay-NBP2-54787-img0007.jpg)
![Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) (Catalog No. NBP2-54787) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Product-Image-NBP2-54787-img0009.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - TEM of Type 2 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54787)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Electron-Microscopy-NBP2-54787-img0008.jpg)
![Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - (1) HeLa, (2) PC12 and (3) NIH-3T3 whole cell lysates were separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with Anti-14-3-3 gamma [Ac Val2] antibody at 2 ug/ml for 1 hour, followed by incubation a 1:10,000 dilution of anti-rabbit HRP. The protein was detected at approximately 28 kDa after incubation with SuperSignal West Pico Chemiluminescent substrate.](http://images.novusbio.com/fullsize/14-3-3-gamma-ac-Val2-Antibody-KC21-Western-Blot-NB100-406-img0005.jpg "Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - (1) HeLa, (2) PC12 and (3) NIH-3T3 whole cell lysates were separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with Anti-14-3-3 gamma [Ac Val2] antibody at 2 ug/ml for 1 hour, followed by incubation a 1:10,000 dilution of anti-rabbit HRP. The protein was detected at approximately 28 kDa after incubation with SuperSignal West Pico Chemiluminescent substrate.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - 14-3-3 gamma [ac Val2] antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/14-3-3-gamma-ac-Val2-Antibody-KC21-Immunocytochemistry-Immunofluorescence-NB100-406-img0004.jpg)
![Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - 14-3-3 gamma detected in lysates using NB100-406. Lane 1: HeLa cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).](http://images.novusbio.com/fullsize/14-3-3-gamma-ac-Val2-Antibody-KC21-Western-Blot-NB100-406-img0002.jpg)
![Simple Western: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - Lane view shows a specific band for 14-3-3 Gamma in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/14-3-3-gamma-ac-Val2-Antibody-KC21-Simple-Western-NB100-406-img0007.jpg)
![Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.](http://images.novusbio.com/fullsize/Abeta-40-Antibody-Western-Blot-NBP1-44047-img0002.jpg "Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.")
![HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PEDF antibody [N1C2] (NBP2-19767) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19767_rabbit-polyclonal-serpin-f1-pedf-antibody-western-blot-261120251839577.jpg "HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PEDF antibody [N1C2] (NBP2-19767) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - Analysis of MECP2 expression in transfected 293T cell line by MECP2 monoclonal antibody (M01), clone 4B6.Lane 1: MECP2 transfected lysate(52.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MeCP2-Antibody-4B6-Western-Blot-H00004204-M01-img0012.jpg "Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - Analysis of MECP2 expression in transfected 293T cell line by MECP2 monoclonal antibody (M01), clone 4B6.Lane 1: MECP2 transfected lysate(52.4 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: MeCP2 Antibody (4B6) [H00004204-M01] - Analysis of monoclonal antibody to MECP2 on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/MeCP2-Antibody-4B6-Immunohistochemistry-Paraffin-H00004204-M01-img0007.jpg)
![Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - MECP2 monoclonal antibody (M01), clone 4B6 Analysis of MECP2 expression in MCF-7.](http://images.novusbio.com/fullsize/MeCP2-Antibody-4B6-Western-Blot-H00004204-M01-img0009.jpg)
![Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - MECP2 monoclonal antibody (M01), clone 4B6. Analysis of MECP2 expression in NIH/3T3.](http://images.novusbio.com/fullsize/MeCP2-Antibody-4B6-Western-Blot-H00004204-M01-img0010.jpg)
![Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - MECP2 monoclonal antibody (M01), clone 4B6. Analysis of MECP2 expression in rat muscle.](http://images.novusbio.com/fullsize/MeCP2-Antibody-4B6-Western-Blot-H00004204-M01-img0011.jpg)
![Sandwich ELISA: MeCP2 Antibody (4B6) [H00004204-M01] - Detection limit for recombinant GST tagged MECP2 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MeCP2-Antibody-4B6-Sandwich-ELISA-H00004204-M01-img0008.jpg)
![Western Blot: GAT3 Antibody [NBP1-91920] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/GAT3-Antibody-Western-Blot-NBP1-91920-img0006.jpg "Western Blot: GAT3 Antibody [NBP1-91920] - Analysis in human cerebral cortex tissue.")
![Immunohistochemistry-Paraffin: GAT3 Antibody [NBP1-91920] - Staining of human Cerebellum shows strong positivity in processes in cells in molecular layer.](http://images.novusbio.com/fullsize/GAT3-Antibody-Immunohistochemistry-Paraffin-NBP1-91920-img0007.jpg)
![Immunohistochemistry-Paraffin: GAT3 Antibody [NBP1-91920] - Staining of human Cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/GAT3-Antibody-Immunohistochemistry-Paraffin-NBP1-91920-img0008.jpg)
![Immunohistochemistry-Paraffin: GAT3 Antibody [NBP1-91920] - Staining of human Testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/GAT3-Antibody-Immunohistochemistry-Paraffin-NBP1-91920-img0010.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody (CL2754) [NBP2-46627] - VGLUT1 Antibody (2754) [NBP2-46627] - Staining in human cerebral cortex and tonsil tissues. Corresponding VGLUT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-2754-Immunohistochemistry-Paraffin-NBP2-46627-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VGLUT1 Antibody (CL2754) [NBP2-46627] - VGLUT1 Antibody (2754) [NBP2-46627] - Staining in human cerebral cortex and tonsil tissues. Corresponding VGLUT1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: VGLUT1 Antibody (2754) [NBP2-46627] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/VGLUT1-Antibody-2754-Western-Blot-NBP2-46627-img0008.jpg)
![Immunohistochemistry: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of rat brain shows strong positivity in glutamatergic processes in the molecular and granular layers of cerebellum.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-2754-Immunohistochemistry-NBP2-46627-img0015.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-2754-Immunohistochemistry-Paraffin-NBP2-46627-img0011.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of human tonsil shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-2754-Immunohistochemistry-Paraffin-NBP2-46627-img0018.jpg)
![Immunohistochemistry: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of mouse hippocampus shows strong positivity in glutamatergic fibers in the CA2 layer.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-2754-Immunohistochemistry-NBP2-46627-img0012.jpg)
![Immunohistochemistry: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of mouse brain shows strong positivity in glutamatergic fibers in the molecular and granular layers of the cerebellum.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-2754-Immunohistochemistry-NBP2-46627-img0013.jpg)
![Immunohistochemistry: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of rat brain shows strong positivity in glutamatergic fibers in the polymorph layer of dentate gyrus.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-2754-Immunohistochemistry-NBP2-46627-img0014.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Analysis in human cerebral cortex and tonsil tissues using NBP2-46629 antibody. Corresponding GAD2/GAD65 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Analysis in human cerebral cortex and tonsil tissues using NBP2-46629 antibody. Corresponding GAD2/GAD65 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Western-Blot-NBP2-46629-img0009.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of mouse midbrain shows moderate positivity in GABergic neural fibers in the substantia nigra.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0014.jpg)
![Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Western-Blot-NBP2-46629-img0008.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of rat basal forebrain shows moderate immunoreactivity in GABAergic fibers.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0001.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of mouse substantia nigra shows moderate positivity in GABAergic fibers in pars reticulata.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0002.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of rat globus pallidus shows moderate positivity in GABAergic neural fibers.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0004.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human cerebellum shows moderate immunoreactivity in GABAergic fibers in granular and molecular layers.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0005.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human cerebral cortex shows moderate to strong positivity in GABAergic neuronal processes.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0011.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human pancreas shows moderate cytoplasmic positivity in endocrine glandular cells.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0012.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0013.jpg)