Neuroscience reagents: Novus Biologicals

NB100-2833

AIF-1/Iba1 Antibody

(11 Publications)

0.1 mg

NB100-2833

Flow Cytometry: AIF-1/Iba1 Antibody [NB100-2833] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Pg/gingipain is present intracellularly in astrocytes, microglia and neurons in the hippocampus of experimental mice.Green: astrocytes (top panels), microglia (middle panels), and neurons (bottom panels). Red: Pg/gingipain. Blue: DAPI. Representative of N = 4 (GFAP), 3 (Iba1) and 4 (NeuN) experimental mice. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0204941), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Immunofluorescence analysis of paraformaldehyde fixed CaCo-2 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-2833] - Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Pg/gingipain is present intracellularly in astrocytes, microglia & neurons in the hippocampus of experimental mice.Green: astrocytes (top panels), microglia (middle panels), & neurons (bottom panels). Red: Pg/gingipain. Blue: DAPI. Representative of N = 4 (GFAP), 3 (Iba1) & 4 (NeuN) experimental mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - IL-1 beta synthesis, & microglial activation modulation in PDD005-treated aged mice. (A) Images showing IL-1 beta (green fluorescence), iba-1 (red fluorescence) expression. Yellow = Overlay; Blue = nuclei; SGZ region of mouse brain. 40X magnification, scale bar = 100 μm. (B) Graphs representing the average relative fluorescence of Iba-1 in SGZ. Significant differences determined by using one-way ANOVA with Tukey’s test. *P > Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26536027), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Ca

Species Glossary

Reactivity: Hu, Mu, Ca

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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NBP1-89236

$DMS 53 cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of ADNP Antibody (Catalog # NBP1-89236) pre-coupled to Dynabeads Protein A. Immunoprecipitated ADNP was detected in Western Blot with a Rabbit ADNP antibody used at 1/500. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).$

Expression of ADNP in SIRC cells exposed to UV-B radiations. (A) Representative immunoblots of ADNP expression in SIRC cells following exposure to UV-B insult. The bar graph shows quantitative analysis of signals obtained by immunoblots resulting from three independent experiments. Relative band densities were quantified using ImageJ software. Protein levels are expressed as arbitrary units obtained following normalization to b-tubulin, which was used as loading control. Data represent means +/- SEM. *** p < 0.001 vs. CTRL, as determined by unpaired two-tailed Student t-test. (B) Representative photomicrographs showing ADNP expression (green) in SIRC cells exposed to UV-B radiations. Nuclei were stained with DAPI. Photomicrographs are representative results of fields taken randomly from each slide and scanned by confocal laser scanning microscopy (CLSM; Zeiss LSM700). In the right column, a thin section (0.3 um) with some SIRC cells showing ADNP in the euchromatic compartment of the nuclei is shown. Scale bar in the left and middle columns is 20 μm, and in the right column is 10 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35052632), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Expression of ADNP and p63 in corneal epithelium. H&E staining and immunodetection of ADNP and p63 in human (A) and rabbit (B) cornea: (1) Epithelium, (2) Bowman’s membrane, (3) stroma. Original magnification ×200. Digital micrographs are representative results of fields taken in randomly selected slides and obtained using the Zeiss Axioplan light microscope (Carl Zeiss) fitted with a digital camera (AxioCam MRc5; Carl Zeiss); scale bar: 20 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35052632), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Rb

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-89236-25ul

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NB100-1533

POMC Antibody

(11 Publications)

0.1 mg

NB100-1533

Immunohistochemistry-Paraffin: POMC Antibody [NB100-1533] - Staining of paraffin embedded Human Pituitary Gland. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Immunohistochemistry: POMC Antibody [NB100-1533] - Representative immunofluorescence images of (B) POMC as a prohormone (n = 5-6 each) in the ARC of mice approximately 5 to 10 weeks of age. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY license.

$Immunocytochemistry/ Immunofluorescence: POMC Antibody [NB100-1533] - Pathogenic POMC-C28F mutation is completely rescued by an intragenic suppressor mutation C50S.(A) Schematic diagram showing the sequence & positions of Cys residues in POMC-WT, C28F, & C28F/C50S. (B) Western blot analyses of POMC-Flag immunoprecipitates in HEK293T cells transfected with a combination of Myc- or Flag-tagged POMC under nonreducing (– beta -ME) & reducing (+ beta -ME) SDS-PAGE. Two panels were from the same experiment at the same exposure time, with the irrelevant lanes in the middle cut off. (C) Sucrose gradient fractionation & Western blot analysis of HEK293T cells expressing POMC-C28F or -C28F/C50S under nonreducing (– beta -ME) & reducing (+ beta -ME) SDS-PAGE. (D) Metabolic labeling experiments to visualize the maturation of nascent POMC in HEK293T cells transfected with POMC-Myc. Cells were pulsed for 30 minutes & chased for the indicated times, followed by immunoprecipitation with anti-Myc agarose beads & separation on SDS-PAGE gels under nonreducing or reducing conditions & autoradiography. (E) Representative confocal images of POMC in POMC-transfected WT N2a cells. White arrows point to secreted POMC in granules, while yellow arrows point to perinuclear POMC, possibly in the form of aggregates. KDEL marks the ER. Representative data from at least 2 independent experiments shown. Image collected & cropped by CiteAb from the following publication (//www.jci.org/articles/view/96420), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt, Po, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Sh

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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NB100-493

PINK1 Antibody - BSA Free

(12 Publications)

0.1 ml

NB100-493

PINK1 was detected in immersion fixed HeLa human cervix adenocarcinoma cell line using Rabbit anti-PINK1 Antigen Affinity Purified Polyclonal Antibody (Catalog # NB100-493) at 1.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-493AF350
Alexa Fluor 405	NB100-493AF405
Alexa Fluor 488	NB100-493AF488
Alexa Fluor 532	NB100-493AF532
Alexa Fluor 594	NB100-493AF594
Alexa Fluor 647	NB100-493AF647
Alexa Fluor 700	NB100-493AF700
Alexa Fluor 750	NB100-493AF750
Allophycocyanin	NB100-493APC
Biotin	NB100-493B
CoraFluor 1	NB100-493CL1
DyLight 350	NB100-493UV
DyLight 405	NB100-493V
DyLight 488	NB100-493G
DyLight 550	NB100-493R
DyLight 594	NB100-493DL594
DyLight 650	NB100-493C
DyLight 680	NB100-493FR
DyLight 755	NB100-493IR
FITC	NB100-493F
HRP	NB100-493H
Janelia Fluor 525	NB100-493JF525
Janelia Fluor 549	NB100-493JF549
Janelia Fluor 646	NB100-493JF646
Janelia Fluor 669	NB100-493JF669
PE	NB100-493PE
PerCP	NB100-493PCP
mFluor Violet 450 SE	NB100-493MFV450
mFluor Violet 500 SE	NB100-493MFV500
mFluor Violet 610 SE	NB100-493MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-493SS

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NB300-324

GAPDH Antibody

(9 Publications)

0.1 ml

NB300-324

Immunohistochemistry: GAPDH Antibody [NB300-324] - Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GAPDH (NB100-79808). Detection: DAB

Western Blot: GAPDH Antibody [NB300-324] - Detection of Human and Mouse GAPDH, theoretical molecular weight 36 kDa. Whole cell lysate from HeLa (5, 15 and 50 mcg) and mouse NIH3T3 (M; 50 mcg). Affinity purified rabbit anti-GAPDH antibody used at 0.04 mcg/ml. Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-324] - IHC detection of GAPDH in formalin-fixed paraffin-embedded human lung carcinoma tissue sections with NB300-324 at a dilution of 1:200

Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-324] - IHC detection of GAPDH in formalin-fixed paraffin-embedded mouse squamous cell carcinoma tissue sections with NB300-324 at a dilution of 1:200.

Simple Western: GAPDH Antibody [NB300-324] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.

Reactivity:

Hu, Mu, Xp, Rt, Po, Am, Av, Ch, Gp, Pm, Re, Tr

Species Glossary

Reactivity: Hu, Mu, Xp, Rt, Po, Am, Av, Ch, Gp, Pm, Re, Tr

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-31239

GPER/GPR30 Antibody - BSA Free

(1 Review)

(9 Publications)

100 ul

NBP1-31239

<b>Genetic Strategies Validation. </b>Western Blot: GPER/GPR30 Antibody [NBP1-31239] - A. 50 ug 293T whole cell lysate/extract. B. 50 ug whole cell lysate/extract of GFP-human GPR30-transfected 293T cells (No boiling). C. 50 ug whole cell lysate/extract of GFP-human GPR30 and GPR30 siRNA-transfected 293T cells (No boiling). 5 % SDS-PAGE GPR30 antibody ) dilution: 1:1000

Western Blot: GPER/GPR30 Antibody [NBP1-31239] - AhR & GPR30 receptors are present & functional in the MCF10AT1 cells. (A) RT-qPCR analysis of AhR & GPR30 mRNA expression levels represented in arbitrary units (a.u.) in the MCF10AT1 & MCF10CA1a.cl1 cells. MCF-7 cells were used as a control. Values represent mean ± SD of three independent experiments conducted in triplicate. (B) Representative Western blot analyses from three independent experiments of AhR & GPR30 protein expression in MCF10AT1 & MCF10CA1a.cl1 cells. MCF-7 cells were used as a control. (C) XRE-luciferase activity following 8 h exposure of MCF10AT1 cells to ITE at the indicated concentrations. TCDD 10−7 M was used as a control & results were expressed as % of TCDD 10−7 M activity. ***p < 0.001 in Student t-test. (D) XRE-luciferase activity upon 8 h of exposure to ITE 10−10 M alone or in combination with GNF351 at the indicated concentrations. TCDD 10−7 M was used as a control, & results were expressed as % of TCDD 10−7 M activity. Student t-tests revealed the statistically significant differences between unexposed & exposed cells: ***p < 0.001; & between ITE & ITE+GNF351: ###p < 0.001. Values in (C,D) represent mean ± SD of three independent experiments. (E) Representative Western blot analyses from three independent experiments of the phospho-MAPK/MAPK ratio upon exposure of MCF10AT1 cells to G1 (GPR30 agonist) for the times indicated, in the presence or absence of a 2 h pre-treatment with G15 (GPR30 antagonist). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32670863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GPER/GPR30 Antibody [NBP1-31239] - Effects of short-term exposure of BPA, B[a]P, ITE & G1 10−10 M on AIG & MFE are inhibited by siRNA-AhR & siRNA-GPR30. Representative Western blot analysis from three independent experiments of AhR & GPR30 expression in transfected MCF10AT1 cells with (A) siRNA-AhR, (B) siRNA-GPR30 or their scrambled controls. Quantification of protein expression levels was normalized against tubulin expression. (C,D) Secondary mammospheres formation & (E,F) average number of colonies in soft agar, with the following treatments: BPA and/or B[a]P, G1, or ITE, 10−10 M. Cells were transfected with either siRNA-AhR, siRNA-GPR30 or their scrambled controls before being subjected to the treatments. Treatments were maintained throughout the course of experiments. (mean ± SD of 2 independent experiments, in triplicate). ***p < 0.001, *p < 0.05 vs. their respective unexposed; ###p < 0.001 siRNA vs. scrambled in Student t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32670863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: GPER/GPR30 Antibody [NBP1-31239] - Human endothelial cells express GPR30 protein in the cell nucleus.(A) Confluent monolayers of HUVECs from 3 different cords were lysed & the protein lysates were immunoblotted for GPR30. alpha -tubulin was used as loading control. (B) HUVEC monolayers at 30–40% confluence were treated with 40 nM siRNA (control or GPR30_4) for 48 hours prior to lysis followed by immunoblotting of the cell lysates for GPR30. alpha -tubulin was used as loading control. Data shown are mean ± SEM of 4 independent experiments. ** & ## indicate p<0.01 compared to untreated & control siRNA-treated cells, respectively. (C) Confluent HUVECs grown on glass coverslips were fixed, permeabilized & immunostained with anti-GPR30 antibody. Nuclei were stained with Hoechst33342 dye. The merged image shows GPR30 (red) & nuclei (blue) in pseudocolor. Representative images from 3 independent experiments are shown. Bar, 20 µm. (D) Confluent HUVECs were lysed & fractionated into cytosolic (C) & nuclear (N) fractions prior to western blotting for eNOS, GPR30, p65, alpha -tubulin & c-Jun. A representative set of images (obtained from different membranes) from 3 independent experiments is shown. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23285008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GPER/GPR30 antibody (NBP1-31239) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Po, Rt

Species Glossary

Reactivity: Hu, Po, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-71656

OPA1 Antibody (1E8-1D9) - BSA Free

(1 Review)

(4 Publications)

0.1 ml

NBP1-71656

Immunohistochemistry: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 on mouse skin using NBP1-71656.

Simple Western: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Image shows a specific band for OPA1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: OPA1 Antibody (1E8-1D9) - BSA Free [NBP1-71656] - Image shows a specific band for OPA1 in 0.1 ug/uL mouse hippocampus tissue lysate. Primary antibody dilution: 1:200. Image from verified customer review.

Melatonin-induced miR-4516 improves mitochondrial dynamics and enhances PINK1/Parkin-mediated mitophagy in the CKD mouse model. (A) Representative TEM images of mitochondria in renal cortex of CKD mice either treated with melatonin (0.2 mg/kg), or both melatonin and miR-4516 inhibitor (300 nM). Each group received two intraperitoneal injections per week (every 3–4 days)—a total of 4 injections for 2 weeks. All comparisons were made against healthy kidney control (scare bar = 1 μm) (B,C) Measurement of mitochondrial area and number of abnormal mitochondria in renal cortex of each groups (n = 3). (D) The expression of p-DRP1, DRP1, MFN1, and OPA1 in renal cortex of each group. Protein expression level were quantified by densitometry and normalized to DRP1 or alpha -tubulin levels (n = 3). (E) Immunofluorescence staining for LAMP-1 (green) and COX4 (red) in renal cortex of each group. Scare bar = 20 μm. (F) Expression of LC3B-II/LC3B-I ratio and P62 in renal cortex of each groups (n = 3). The values represent mean +/- SEM, * p < 0.05, ** p < 0.01 versus healthy kidney cortex; #p < 0.05, ##p < 0.01 versus PBS; $p < 0.05, $$p < 0.01 versus melatonin. The alpha -tubulin was used as Western blot loading control for whole tissue lysates. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34359852), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Melatonin-induced miR-4516 rescues abnormal mitochondrial functions. (A) Representative TEM images for TH1 cells either treated with p-Cresol alone, melatonin under p-Cresol exposure, or miR-4516 inhibitor (50 nM for 48 h) before melatonin treatment, compared with TH1 control (scare bar = 1 μm). (B,C) Measurement of mitochondrial area and number of abnormal mitochondria in each experimental group (n = 3). (D) The effects of melatonin on p-DRP1, DRP1, MFN1, and OPA1 were reversed with miR-4516 inhibitor. Protein expression level was detected using western blot, quantified by densitometry, and normalized to DRP1 or VDAC1 levels (n = 3) respectively. (E,F) Measurement of TMRE (E) and MitoSOX (F) positive cells for each group (n = 3). The values represent mean +/- SEM, * p < 0.05, ** p < 0.01 versus control; #p < 0.05, ##p < 0.01 versus p-Cresol exposure; $$p < 0.01 versus melatonin-treated cells in p-Cresol exposure. The beta -actin or VDAC1 was used as Western blot loading control for whole cell lysates or mitochondrial fraction, respectively. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34359852), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt, Bv, ChHa

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, ChHa

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-71656AF350
Alexa Fluor 405	NBP1-71656AF405
Alexa Fluor 488	NBP1-71656AF488
Alexa Fluor 532	NBP1-71656AF532
Alexa Fluor 594	NBP1-71656AF594
Alexa Fluor 647	NBP1-71656AF647
Alexa Fluor 700	NBP1-71656AF700
Alexa Fluor 750	NBP1-71656AF750
Allophycocyanin	NBP1-71656APC
Biotin	NBP1-71656B
CoraFluor 1	NBP1-71656CL1
DyLight 350	NBP1-71656UV
DyLight 405	NBP1-71656V
DyLight 488	NBP1-71656G
DyLight 550	NBP1-71656R
DyLight 594	NBP1-71656DL594
DyLight 650	NBP1-71656C
DyLight 680	NBP1-71656FR
DyLight 755	NBP1-71656IR
FITC	NBP1-71656F
HRP	NBP1-71656H
Janelia Fluor 525	NBP1-71656JF525
Janelia Fluor 549	NBP1-71656JF549
Janelia Fluor 646	NBP1-71656JF646
Janelia Fluor 669	NBP1-71656JF669
PE	NBP1-71656PE
PerCP	NBP1-71656PCP
mFluor Violet 450 SE	NBP1-71656MFV450
mFluor Violet 500 SE	NBP1-71656MFV500
mFluor Violet 610 SE	NBP1-71656MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-71656SS

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NBP2-16908

AIF-1/Iba1 Antibody

(7 Publications)

0.1 ml

NBP2-16908

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - Primary murine microglia cells, staining with AIF-1:Iba1 Antibody using 1. 0 ug per 4 x 10^5 cells. NBP2-16908 (blue), Rabbit IgG (green) Unstained (red).

Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 antibody (NBP2-16908) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Iba1 stained by Iba1 antibody (NBP2-16908) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Iba1 stained by Iba1 antibody (NBP2-16908) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Iba1 stained by Iba1 antibody (NBP2-16908) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Iba1 stained by Iba1 antibody (NBP2-16908) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (NBP2-16908) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Miconazole effects on NO generation and inflammation in LPS-treated cultured astrocytes and microglial BV2 cells.The NO generation levels were measured in 24-h LPS-treated (1 μg/mL) cultured astrocytes (a) and microglial BV2 cells (b) pretreated with MCZ (1.25, 2.5, 5, and 10 μM) for 1 h. COX-2, GFAP, and Iba-1 proteins were detected by western blotting using specific antibodies in cultured astrocytes (c) and microglial BV2 cells (d). beta -actin levels were measured to confirm equal protein loading. All experiments were performed three times with duplicate. The values on the western blot bands represent the arbitrary density measured by ImageJ. Significant difference from the control group (t-test, p < 0.05). #Significant difference from the LPS-treated group (p < 0.05). *Significant difference from the MCZ-treated group (p < 0.05). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32796824), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, RM

Species Glossary

Reactivity: Hu, Mu, Rt, Po, RM

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-47254

ERR alpha/NR3B1 Antibody - BSA Free

(7 Publications)

0.1 ml

NBP1-47254

Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha/ESRRA protein in a section of normal human kidney using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. Some cells of tubules/ducts in the tested renal tissue depicted intense nuclear staining with an overall weak/diffused cytoplasmic staining. (10X Magnification)

Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - Analysis of ERR alpha in A431 cell lysates

Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - ERR alpha antibody was tested in HeLa cells at a 1:100 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.

Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha / ESRRA protein in a section of normal skin of human using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. The different cell types in the cellular epidermis as well as the blood vessels in the dermal layer showed positivity for ERR alpha, and the staining was found to be more intense in the nuclei compared to cytoplasm. (10X Magnification)

Reactivity:

Hu, Mu, Rt, Po, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ca

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-47254AF350
Alexa Fluor 405	NBP1-47254AF405
Alexa Fluor 488	NBP1-47254AF488
Alexa Fluor 532	NBP1-47254AF532
Alexa Fluor 594	NBP1-47254AF594
Alexa Fluor 647	NBP1-47254AF647
Alexa Fluor 700	NBP1-47254AF700
Alexa Fluor 750	NBP1-47254AF750
Allophycocyanin	NBP1-47254APC
Biotin	NBP1-47254B
CoraFluor 1	NBP1-47254CL1
DyLight 350	NBP1-47254UV
DyLight 405	NBP1-47254V
DyLight 488	NBP1-47254G
DyLight 550	NBP1-47254R
DyLight 594	NBP1-47254DL594
DyLight 650	NBP1-47254C
DyLight 680	NBP1-47254FR
DyLight 755	NBP1-47254IR
FITC	NBP1-47254F
HRP	NBP1-47254H
Janelia Fluor 525	NBP1-47254JF525
Janelia Fluor 549	NBP1-47254JF549
Janelia Fluor 646	NBP1-47254JF646
Janelia Fluor 669	NBP1-47254JF669
PE	NBP1-47254PE
PerCP	NBP1-47254PCP
mFluor Violet 450 SE	NBP1-47254MFV450
mFluor Violet 500 SE	NBP1-47254MFV500
mFluor Violet 610 SE	NBP1-47254MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-47254SS

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NBP1-92695

TDP-43/TARDBP Antibody (3H8) - BSA Free

(7 Publications)

0.1 ml

NBP1-92695

Immunohistochemistry Free-Floating: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of rat hippocampus section stained with mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in red, and costained with chicken pAb to GFAP,, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The TDP43 protein is concentrated in the nuclei of hippocampal neurons, while the GFAP antibody stains the intermediate filament network of astroglial cells.

<b>Genetic Strategies Validation. </b>Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Lysates of HAP1 (WT) and TDP-43/TARDBP (KO) were prepared, and 50 ug of protein was processed for western blot with the TDP-43/TARDBP antibody. The Ponceau stained transfers of the blot is shown (right). Antibody dilution used: NBP1-92695 at 1/1000. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).

Immunoprecipitation: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - HAP1 lysates were prepared, and immunoprecipitation was performed using 2.0 ug of the TDP-43/TARDBP antibody pre-coupled to Dynabeads protein G. Sample was washed and processed for immunoblot with the TDP-43/TARDBP antibody. The Ponceau stained transfer of the blot is shown. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).

<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - HAP1 (WT) and TDP-43/TARDBP (KO) cells were labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with glass bottom. Cells were stained with the TDP-43/TARDBP antibody and with the corresponding Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined on both channels with green and magenta dashed line, respectively. Antibody dilution used: NBP1-92695 at 1/1000. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).

Reactivity:

Hu, Mu, Rt, Po, Bv, Eq

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Eq

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-92695AF350
Alexa Fluor 405	NBP1-92695AF405
Alexa Fluor 488	NBP1-92695AF488
Alexa Fluor 532	NBP1-92695AF532
Alexa Fluor 594	NBP1-92695AF594
Alexa Fluor 647	NBP1-92695AF647
Alexa Fluor 700	NBP1-92695AF700
Alexa Fluor 750	NBP1-92695AF750
Allophycocyanin	NBP1-92695APC
Biotin	NBP1-92695B
CoraFluor 1	NBP1-92695CL1
DyLight 350	NBP1-92695UV
DyLight 405	NBP1-92695V
DyLight 488	NBP1-92695G
DyLight 550	NBP1-92695R
DyLight 594	NBP1-92695DL594
DyLight 650	NBP1-92695C
DyLight 680	NBP1-92695FR
DyLight 755	NBP1-92695IR
FITC	NBP1-92695F
HRP	NBP1-92695H
Janelia Fluor 525	NBP1-92695JF525
Janelia Fluor 549	NBP1-92695JF549
Janelia Fluor 646	NBP1-92695JF646
Janelia Fluor 669	NBP1-92695JF669
PE	NBP1-92695PE
PerCP	NBP1-92695PCP
mFluor Violet 450 SE	NBP1-92695MFV450
mFluor Violet 500 SE	NBP1-92695MFV500
mFluor Violet 610 SE	NBP1-92695MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-92695SS

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NBP1-31123

Apolipoprotein E/ApoE Antibody - BSA Free

(4 Publications)

0.1 mg

NBP1-31123

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Apolipoprotein E antibody [C2C3], C-term detects secreted Apolipoprotein E protein by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (NBP1-31123).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Apolipoprotein E/ApoE Antibody [NBP1-31123] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of Apolipoprotein E protein expressing HepG2 cells B : Control with 3ug of pre-immune rabbit IgG C : Immunoprecipitation of Apolipoprotein E by 3 ug of Apolipoprotein E antibody [C2C3], C-term 10% SDS-PAGE The immunoprecipitated Apolipoprotein E protein was detected by Apolipoprotein E antibody [C2C3], C-term diluted at 1 : 1000. Easy Blot anti-rabbit IgG (HRP) was used as a secondary reagent.

Western Blot: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Human tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [C2C3], C-term diluted at 1:5000.

Western Blot: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Sample (2. 5 ug of whole cell lysate) A: Conditional medium from human primary preadipocyte B: Conditional medium from differentiated human primary preadipocyte 12% SDS PAGE, antibody diluted at 1:2000.

Immunocytochemistry/Immunofluorescence: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by analysis. Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min. Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Human hepatoma. Apolipoprotein E antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ELISA: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Sandwich ELISA detection of recombinant full-length Apolipoprotein E protein using as capture antibody at concentration of 5 ug/mL and a mouse monoclonal anti-Apolipoprotein E antibody as detection antibody at concentration of 1 ug/mL. Mouse IgG antibody (HRP) (NBP2-19382) was diluted at 1:10000 and used to detect the primary antibody.

Apolipoprotein E/ApoE was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Rabbit anti-Apolipoprotein E/ApoE Affinity Purified Polyclonal Antibody conjugated to Biotin (Catalog # NBP1-31123B) at 5 µg/mL overnight at 4C. Cells were stained using Streptavidin conjugated to DyLight 550 (red) and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Pm

Species Glossary

Reactivity: Hu, Pm

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-31123AF350
Alexa Fluor 405	NBP1-31123AF405
Alexa Fluor 488	NBP1-31123AF488
Alexa Fluor 532	NBP1-31123AF532
Alexa Fluor 594	NBP1-31123AF594
Alexa Fluor 647	NBP1-31123AF647
Alexa Fluor 700	NBP1-31123AF700
Alexa Fluor 750	NBP1-31123AF750
Allophycocyanin	NBP1-31123APC
Biotin	NBP1-31123B
CoraFluor 1	NBP1-31123CL1
DyLight 350	NBP1-31123UV
DyLight 405	NBP1-31123V
DyLight 488	NBP1-31123G
DyLight 550	NBP1-31123R
DyLight 594	NBP1-31123DL594
DyLight 650	NBP1-31123C
DyLight 680	NBP1-31123FR
DyLight 755	NBP1-31123IR
FITC	NBP1-31123F
HRP	NBP1-31123H
Janelia Fluor 525	NBP1-31123JF525
Janelia Fluor 549	NBP1-31123JF549
Janelia Fluor 646	NBP1-31123JF646
Janelia Fluor 669	NBP1-31123JF669
PE	NBP1-31123PE
PerCP	NBP1-31123PCP
mFluor Violet 450 SE	NBP1-31123MFV450
mFluor Violet 500 SE	NBP1-31123MFV500
mFluor Violet 610 SE	NBP1-31123MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-31123SS

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NBP2-59770

OPA1 Antibody (1284B) - BSA Free

(1 Review)

(8 Publications)

0.1 mg

NBP2-59770

Immunohistochemistry-Paraffin: OPA1 Antibody (1284B) [NBP2-59770] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of mouse testes using OPA1 antibody at 1:1000 dilution. The primary antibody bound to OPA1 antigens in the tissue section was deteced using an HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This OPA1 antibody generated a diffused cytoplasmic staining of OPA1 protein in the tubular epithelial cells, the spermatids/spermatocytes and Leydig's cells.

Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1284B) [NBP2-59770] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-OPA1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-59770AF350
Alexa Fluor 405	NBP2-59770AF405
Alexa Fluor 488	NBP2-59770AF488
Alexa Fluor 532	NBP2-59770AF532
Alexa Fluor 594	NBP2-59770AF594
Alexa Fluor 647	NBP2-59770AF647
Alexa Fluor 700	NBP2-59770AF700
Alexa Fluor 750	NBP2-59770AF750
Allophycocyanin	NBP2-59770APC
Biotin	NBP2-59770B
CoraFluor 1	NBP2-59770CL1
DyLight 350	NBP2-59770UV
DyLight 405	NBP2-59770V
DyLight 488	NBP2-59770G
DyLight 550	NBP2-59770R
DyLight 594	NBP2-59770DL594
DyLight 650	NBP2-59770C
DyLight 680	NBP2-59770FR
DyLight 755	NBP2-59770IR
FITC	NBP2-59770F
HRP	NBP2-59770H
Janelia Fluor 525	NBP2-59770JF525
Janelia Fluor 549	NBP2-59770JF549
Janelia Fluor 646	NBP2-59770JF646
Janelia Fluor 669	NBP2-59770JF669
PE	NBP2-59770PE
PerCP	NBP2-59770PCP
mFluor Violet 450 SE	NBP2-59770MFV450
mFluor Violet 500 SE	NBP2-59770MFV500
mFluor Violet 610 SE	NBP2-59770MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-59770SS
Azide and BSA Free	NBP2-80894

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

AIF-1/Iba1 Antibody

ADNP Antibody - BSA Free

POMC Antibody

PINK1 Antibody - BSA Free

GAPDH Antibody

GPER/GPR30 Antibody - BSA Free

OPA1 Antibody (1E8-1D9) - BSA Free

AIF-1/Iba1 Antibody

ERR alpha/NR3B1 Antibody - BSA Free

TDP-43/TARDBP Antibody (3H8) - BSA Free

Apolipoprotein E/ApoE Antibody - BSA Free

OPA1 Antibody (1284B) - BSA Free