| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08442 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48703-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03109-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03718-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15492-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22411-0.025mg | |||
![Western Blot: DISC1 Antibody (4U5N1) [NBP3-16462] - Western blot analysis of extracts of various cell lines, using DISC1 Rabbit mAb (NBP3-16462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/DISC1-Antibody-4U5N1-Western-Blot-NBP3-16462-img0004.jpg "Western Blot: DISC1 Antibody (4U5N1) [NBP3-16462] - Western blot analysis of extracts of various cell lines, using DISC1 Rabbit mAb (NBP3-16462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: DISC1 Antibody (4U5N1) [NBP3-16462] - Immunofluorescence analysis of U-2 OS cells using DISC1 Rabbit mAb (NBP3-16462) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DISC1-Antibody-4U5N1-Immunocytochemistry-Immunofluorescence-NBP3-16462-img0003.jpg)
![Western Blot: DISC1 Antibody (4U5N1) [NBP3-16462] - Western blot analysis of extracts of various cell lines, using DISC1 Rabbit mAb (NBP3-16462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/DISC1-Antibody-4U5N1-Western-Blot-NBP3-16462-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: DISC1 Antibody (4U5N1) [NBP3-16462] - Immunofluorescence analysis of C6 cells using DISC1 Rabbit mAb (NBP3-16462) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DISC1-Antibody-4U5N1-Immunocytochemistry-Immunofluorescence-NBP3-16462-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DISC1 Antibody (4U5N1) [NBP3-16462] - Immunofluorescence analysis of NIH-3T3 cells using DISC1 Rabbit mAb (NBP3-16462) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DISC1-Antibody-4U5N1-Immunocytochemistry-Immunofluorescence-NBP3-16462-img0002.jpg)
![Western Blot: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Syntenin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Syntenin 1.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-OTI2H6-Azide-and-BSA-Free-Western-Blot-NBP2-74418-img0002.jpg "Western Blot: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Syntenin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Syntenin 1.")
![Immunohistochemistry: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Syntenin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-OTI2H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74418-img0004.jpg)
![Western Blot: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-Syntenin 1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-OTI2H6-Azide-and-BSA-Free-Western-Blot-NBP2-74418-img0001.jpg)
![Immunohistochemistry: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Syntenin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-OTI2H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74418-img0003.jpg)
![Immunohistochemistry: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - Staining of paraffin-embedded Human liver tissue using anti-Syntenin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-OTI2H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74418-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody [NBP1-31136] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Syntenin 1antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31136-img0011.jpg "Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody [NBP1-31136] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Syntenin 1antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.")
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-31136] - Mouse liver. Syntenin 1 antibody [N1], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-31136-img0005.jpg)
![Immunoprecipitation: Syntenin 1 Antibody [NBP1-31136] - Immunoprecipitation of Syntenin 1 protein from U87-MG whole cell extracts using 5 ug of Syntenin 1 antibody [N1], N-term (NBP1-31136). Western blot analysis was performed using Syntenin 1 antibody [N1], N-term (NBP1-31136).EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunoprecipitation-NBP1-31136-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody [NB110-58783] - Spectrin beta 3 antibody was tested in Neuro 2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58783-img0002.jpg "Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody [NB110-58783] - Spectrin beta 3 antibody was tested in Neuro 2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry: Spectrin beta 3 Antibody [NB110-58783] - Detection of Spectrin Beta-III in human hepatocytes using NB110-58783.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-Immunohistochemistry-NB110-58783-img0006.jpg)
![Immunoprecipitation: Spectrin beta 3 Antibody [NB110-58783] - Detection of Human SPTBN2/SCA5 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SPTBN2/SCA5 antibody NB110-58783 used for IP at 3 mcg/mg lysate. SPTBN2/SCA5 was also immunoprecipitated by rabbit anti-SPTBN2/SCA5 antibody NB110-58346, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-Immunoprecipitation-NB110-58783-img0005.jpg)
![Western Blot: Spectrin beta 3 Antibody (SPTBN2/3142R) [NBP3-07294] - Western Blot Analysis of HeLa, PC-3 cells and Brain tissue lysate using Spectrin beta 3 Recombinant Rabbit Monoclonal (SPTBN2/3142R).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-3142R-Western-Blot-NBP3-07294-img0004.jpg "Western Blot: Spectrin beta 3 Antibody (SPTBN2/3142R) [NBP3-07294] - Western Blot Analysis of HeLa, PC-3 cells and Brain tissue lysate using Spectrin beta 3 Recombinant Rabbit Monoclonal (SPTBN2/3142R).")
![Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (SPTBN2/3142R) [NBP3-07294] - Immunofluorescence Analysis of HeLa cells labeling Spectrin Beta III with Spectrin beta 3 Recombinant Rabbit Monoclonal (SPTBN2/3142R) followed by goat anti- Mouse IgG-CF488 (Green). Nuclear counterstain is RedDot (Red)](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-3142R-Immunocytochemistry-Immunofluorescence-NBP3-07294-img0002.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/3142R) [NBP3-07294] - Formalin-fixed, paraffin-embedded human Pancreas stained with Spectrin beta 3 Recombinant Rabbit Monoclonal (SPTBN2/3142R).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-3142R-Immunohistochemistry-Paraffin-NBP3-07294-img0003.jpg)
![Flow Cytometry: Spectrin beta 3 Antibody (SPTBN2/3142R) [NBP3-07294] - Flow Cytometric Analysis of HeLa cells using Spectrin beta 3 Recombinant Rabbit Monoclonal (SPTBN2/3142R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-3142R-Flow-Cytometry-NBP3-07294-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody [NBP2-48703] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48703-img0003.jpg "Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody [NBP2-48703] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody [NBP2-48703] - Staining in human skin and endometrium tissues using anti-SPTBN2 antibody. Corresponding SPTBN2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-48703-img0006.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody [NBP2-48703] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells, granular and molecular layer cells.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-48703-img0002.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody [NBP2-48703] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-48703-img0004.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody [NBP2-48703] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-48703-img0005.jpg)
![Western Blot: Kv11.1 Antibody [NBP3-03109] - Analysis of extracts of various cell lines, using Kv11.1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhan](http://images.novusbio.com/fullsize/Kv11.1-Antibody-Western-Blot-NBP3-03109-img0004.jpg "Western Blot: Kv11.1 Antibody [NBP3-03109] - Analysis of extracts of various cell lines, using Kv11.1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhan")
![Immunohistochemistry-Paraffin: Kv11.1 Antibody [NBP3-03109] - Human lung cancer using KCNH2 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kv11.1-Antibody-Immunohistochemistry-Paraffin-NBP3-03109-img0003.jpg)
![Immunohistochemistry-Paraffin: Kv11.1 Antibody [NBP3-03109] - human breast cancer using KCNH2 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kv11.1-Antibody-Immunohistochemistry-Paraffin-NBP3-03109-img0001.jpg)
![Immunohistochemistry-Paraffin: Kv11.1 Antibody [NBP3-03109] - Human colon carcinoma using KCNH2 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kv11.1-Antibody-Immunohistochemistry-Paraffin-NBP3-03109-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Kv11.1 Antibody - Azide and BSA Free [Kv11.1] - Immunofluorescence analysis of Mouse kidney tissue using Kv11.1 Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-03109_rabbit-polyclonal-kv11-1-antibody-52202516293929.jpg)
![Western Blot: Galactosylceramidase/GALC Antibody [NBP3-03718] - Analysis of extracts of various cell lines, using Galactosylceramidase/GALC antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Galactosylceramidase-GALC-Antibody-Western-Blot-NBP3-03718-img0004.jpg "Western Blot: Galactosylceramidase/GALC Antibody [NBP3-03718] - Analysis of extracts of various cell lines, using Galactosylceramidase/GALC antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry-Paraffin: Galactosylceramidase/GALC Antibody [NBP3-03718] -Human kidney using GALC Rabbit pAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-03718_rabbit-polyclonal-galactosylceramidase-galc-antibody-immunohistochemistry-paraffin-682024111037..jpg)
![Immunocytochemistry/ Immunofluorescence: Galactosylceramidase/GALC Antibody - BSA Free [Galactosylceramidase/GALC] - Immunofluorescence analysis of A-549 cells using Galactosylceramidase/GALC Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03718_rabbit-polyclonal-galactosylceramidase-galc-antibody-5220251638083.jpg)
![Western Blot: Huntingtin Antibody [NBP3-15492] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Western-Blot-NBP3-15492-img0003.jpg "Western Blot: Huntingtin Antibody [NBP3-15492] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Huntingtin Antibody [NBP3-15492] - Immunohistochemistry of paraffin-embedded rat brain using Huntingtin Rabbit pAb (NBP3-15492) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Immunohistochemistry-Paraffin-NBP3-15492-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Huntingtin Antibody - Azide and BSA Free [Huntingtin] - Immunofluorescence analysis of U-251MG cells using Huntingtin Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15492_rabbit-huntingtin-pab-5220251734580.jpg)
![Western Blot: Kif4A Antibody (4A9P2) [NBP3-16748] - Western blot analysis of extracts of various cell lines, using Kif4A antibody (NBP3-16748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Kif4A-Antibody-4A9P2-Western-Blot-NBP3-16748-img0004.jpg "Western Blot: Kif4A Antibody (4A9P2) [NBP3-16748] - Western blot analysis of extracts of various cell lines, using Kif4A antibody (NBP3-16748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748] - Immunohistochemistry of paraffin-embedded mouse testis using Kif4A Rabbit mAb (NBP3-16748) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kif4A-Antibody-4A9P2-Immunohistochemistry-Paraffin-NBP3-16748-img0003.jpg)
![Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748] - Immunohistochemistry of paraffin-embedded rat testis using Kif4A Rabbit mAb (NBP3-16748) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kif4A-Antibody-4A9P2-Immunohistochemistry-Paraffin-NBP3-16748-img0001.jpg)
![Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748] - Immunohistochemistry of paraffin-embedded human colon using Kif4A Rabbit mAb (NBP3-16748) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kif4A-Antibody-4A9P2-Immunohistochemistry-Paraffin-NBP3-16748-img0002.jpg)
![Western Blot: Stargazin Antibody (S245-36) [NBP2-22411] - Western Blot analysis of Rat brain lysates showing detection of StargazinCalcium Channel protein using Mouse Anti-StargazinCalcium Channel Monoclonal Antibody, Clone S245-36 (NBP2-22411). Primary Antibody: Mouse Anti-StargazinCalcium Channel Monoclonal Antibody (NBP2-22411) at 1:100, 1:250, 1:500, and 1:1000.](http://images.novusbio.com/fullsize/Stargazin-Antibody-S245-36-Western-Blot-NBP2-22411-img0003.jpg "Western Blot: Stargazin Antibody (S245-36) [NBP2-22411] - Western Blot analysis of Rat brain lysates showing detection of StargazinCalcium Channel protein using Mouse Anti-StargazinCalcium Channel Monoclonal Antibody, Clone S245-36 (NBP2-22411). Primary Antibody: Mouse Anti-StargazinCalcium Channel Monoclonal Antibody (NBP2-22411) at 1:100, 1:250, 1:500, and 1:1000.")
![Immunocytochemistry/Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone S245-36 (NBP2-22411). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (NBP2-22411) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody (D) Composite.](http://images.novusbio.com/fullsize/Stargazin-Antibody-S245-36-Immunocytochemistry-Immunofluorescence-NBP2-22411-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/Stargazin-Antibody-S245-36-Immunocytochemistry-Immunofluorescence-NBP2-22411-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone S245-36 (NBP2-22411). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (NBP2-22411) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Stargazin-Antibody-S245-36-Immunocytochemistry-Immunofluorescence-NBP2-22411-img0005.jpg)
![Western Blot: KIF3A Antibody (JE31-39) [NBP3-32501] - Western blot analysis of KIF3A on different lysates with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (40 ug/Lane) Lane 3: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32501) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-278202415295563.jpg "Western Blot: KIF3A Antibody (JE31-39) [NBP3-32501] - Western blot analysis of KIF3A on different lysates with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (40 ug/Lane) Lane 3: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32501) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: KIF3A Antibody (JE31-39) [NBP3-32501] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32501) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-298202413585569.jpg)
![Immunocytochemistry/ Immunofluorescence: KIF3A Antibody (JE31-39) [NBP3-32501] - Immunocytochemistry analysis of NIH/3T3 cells labeling KIF3A with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-29202410334094.jpg)