| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87334-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12904-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-68123SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74570SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92711SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33186 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86680-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74245 | |||
![Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Western-Blot-NBP1-87334-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87334-img0028.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining in human cerebral cortex and liver tissues . Corresponding UCHL1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0027.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Western-Blot-NBP1-87334-img0017.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Western-Blot-NBP1-87334-img0018.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons and in neuropil.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0022.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0023.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human liver shows no positivity in hepatocytes, as expected.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0024.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0025.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human rectum shows strong cytoplasmic positivity in peripheral ganglion and peripheral nerves.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0026.jpg)
![Western Blot: Nav1.7 Antibody (N68/6) [NBP2-12904] - Western Blot analysis of Hamster CHO cells showing detection of Nav1.7 Sodium Channel protein using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone N68/6 (NBP2-12904). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (NBP2-12904) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Nav1-7-Antibody-N68-6-Western-Blot-NBP2-12904-img0013.jpg "Western Blot: Nav1.7 Antibody (N68/6) [NBP2-12904] - Western Blot analysis of Hamster CHO cells showing detection of Nav1.7 Sodium Channel protein using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone N68/6 (NBP2-12904). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (NBP2-12904) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: Nav1.7 Antibody (N68/6) [NBP2-12904] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav1.7 Monoclonal Antibody, Clone N68/6 (NBP2-12904). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Nav1.7 Monoclonal Antibody (NBP2-12904) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Nav1.7 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Nav1-7-Antibody-N68-6-Immunocytochemistry-Immunofluorescence-NBP2-12904-img0017.jpg)
![Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904] - Immunohistochemistry analysis using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone N68/6 (NBP2-12904). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (NBP2-12904) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Nav1-7-Antibody-N68-6-Immunohistochemistry-NBP2-12904-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Nav1.7 Antibody (N68/6) [NBP2-12904] - Human hippocampal sections using this antibody.](http://images.novusbio.com/fullsize/Nav1-7-Antibody-N68-6-Immunocytochemistry-Immunofluorescence-NBP2-12904-img0007.jpg)
![Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904] - Immunohistochemistry analysis using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone N68/6 (NBP2-12904). Tissue: Brain Slice. Species: Mouse. Fixation: Frozen sections. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (NBP2-12904) at 1:1000. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain.](http://images.novusbio.com/fullsize/Nav1-7-Antibody-N68-6-Immunohistochemistry-NBP2-12904-img0014.jpg)
![Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904] - Immunohistochemistry analysis using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone N68/6 (NBP2-12904). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (NBP2-12904) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Nav1-7-Antibody-N68-6-Immunohistochemistry-NBP2-12904-img0015.jpg)
![Western Blot: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of NGF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysateLane 2: HL-60 cell lysate](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Western-Blot-NBP2-67406-img0007.jpg "Western Blot: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of NGF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysateLane 2: HL-60 cell lysate")
![Immunocytochemistry/Immunofluorescence: beta-NGF Antibody (SI79-01) [NBP2-67406] - Staining NGF in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunocytochemistry-Immunofluorescence-NBP2-67406-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-beta-NGF antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-29, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunohistochemistry-Paraffin-NBP2-67406-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: beta-NGF Antibody (SI79-01) [NBP2-67406] - Staining NGF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunocytochemistry-Immunofluorescence-NBP2-67406-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: beta-NGF Antibody (SI79-01) [NBP2-67406] - Staining NGF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunocytochemistry-Immunofluorescence-NBP2-67406-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of paraffin-embedded mouse brain tissue using anti-NGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunohistochemistry-Paraffin-NBP2-67406-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of paraffin-embedded mouse liver tissue using anti-NGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunohistochemistry-Paraffin-NBP2-67406-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of paraffin-embedded mouse thymus tissue using anti-NGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunohistochemistry-Paraffin-NBP2-67406-img0006.jpg)
![Western Blot: CHT1 Antibody (62-2E8) [NB110-74570] - Western Blot Image of anti-CHT1 (62-2E8). Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:500 dilution of anti-CHT1 in 1% milk/TBST. Reactivity was detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CHT1-Antibody-62-2E8-Western-Blot-NB110-74570-img0003.jpg "Western Blot: CHT1 Antibody (62-2E8) [NB110-74570] - Western Blot Image of anti-CHT1 (62-2E8). Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:500 dilution of anti-CHT1 in 1% milk/TBST. Reactivity was detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry: CHT1 Antibody (62-2E8) [NB110-74570] - Staining of neurons in rat basal forebrain](http://images.novusbio.com/fullsize/CHT1-Antibody-62-2E8-Immunohistochemistry-NB110-74570-img0002.jpg)
![Immunohistochemistry Free-Floating: MAP2 Antibody (5H11) [NBP1-92711] - Analysis of rat hippocampus section stained with mouse mAb to MAP2, NBP1-92711, dilution 1:5,000 in green, and costained with rabbit pAb to alpha-internexin, dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP1-92711 antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, and the alpha-internexin antibody selectively stains axons and dendrites of neuronal cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-5H11-Immunocytochemistry-Immunofluorescence-NBP1-92711-img0001.jpg "Immunohistochemistry Free-Floating: MAP2 Antibody (5H11) [NBP1-92711] - Analysis of rat hippocampus section stained with mouse mAb to MAP2, NBP1-92711, dilution 1:5,000 in green, and costained with rabbit pAb to alpha-internexin, dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP1-92711 antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, and the alpha-internexin antibody selectively stains axons and dendrites of neuronal cells.")
![Western Blot: MAP2 Antibody (5H11) [NBP1-92711] - Analysis of different tissue lysates using mouse mAb to MAP2A/B (5H11), NBP1-92711, dilution 1:10,000 in green: [1] protein standard (red), [2] adult rat whole brain, [2] embryonic (E20) rat brain, [4] adult rat spinal cord, and [5] adult mouse brain lysate. A band observed at about 280 kDa corresponds to full length MAP2a and MAP2B protein. MAP2A/B is expressed heavily in brain particularly in cortical regions, but is a more minor component of spinal cord. Note that the epitope for this antibody in within the projection domain, and so the antibody does not bind to the lower molecular weight MAP2C and MAP2D isoforms which lack this region.](http://images.novusbio.com/fullsize/MAP2-Antibody-5H11-Western-Blot-NBP1-92711-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (5H11) [NBP1-92711] - Single cultured astrocytes stained with MAP2 (red) and Myelin (green). Image from a verified customer review.](http://images.novusbio.com/fullsize/MAP2-Antibody-5H11-Immunocytochemistry-Immunofluorescence-NBP1-92711-img0003.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP2-42847-img0010.jpg "Western Blot: GAP-43 Antibody [NBP2-42847] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP2-42847] - GAP-43 antibody detects GAP-43 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAP-43 protein stained by GAP-43 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42847-img0011.jpg)
![Immunohistochemistry-Frozen: GAP-43 Antibody [NBP2-42847] - GAP-43 antibody detects GAP-43 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: GAP-43 protein stained by GAP-43 antibody diluted at 1:250. Red: NeuN, stained by NeuN antibody diluted at 1:500.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunohistochemistry-Frozen-NBP2-42847-img0007.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP2-42847-img0006.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP2-42847-img0008.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP2-42847-img0009.jpg)
![Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP2-42847] - Cal27 xenograft. GAP43 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunohistochemistry-Paraffin-NBP2-42847-img0002.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Human tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody (NBP2-42847) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-42847_rabbit-polyclonal-gap-43-antibody-91020242021945.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (13C4)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-13C4-Western-Blot-NBP2-29420-img0007.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (13C4)")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (13C4) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-13C4-Immunocytochemistry-Immunofluorescence-NBP2-29420-img0006.jpg)
![Simple Western: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Simple Western lane view shows a specific band for PGP9.5 / UCHL-1 in 0.2 mg/ml of h. Cerebellum (left) and IMR-32 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-13C4-Simple-Western-NBP2-29420-img0001.jpg)
![Simple Western: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Electropherogram image of the corresponding Simple Western lane. PGP9.5 / UCHL-1 antibody was used at 10 ug/ml dilution of h. Cerebellum and IMR-32 lysates(s) respectively.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-13C4-Simple-Western-NBP2-29420-img0004.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NB110-58874] - Analysis of different tissue and cell lysates using rabbit pAb to UCHL1, NB110-58874, dilution 1:2,000 in red, and mouse mAb to HSP60, dilution 1:10,000, in green: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The single band at 24kDa corresponds to the UCHL1 protein, while the 60kDa band represents HSP60 protein. UCHL1 is detectable in CNS extracts and cells with neuronal properties but not in HeLa, NIH-3T3 and other non-neuronal cells.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Western-Blot-NB110-58874-img0006.jpg "Western Blot: UCH-L1/PGP9.5 Antibody [NB110-58874] - Analysis of different tissue and cell lysates using rabbit pAb to UCHL1, NB110-58874, dilution 1:2,000 in red, and mouse mAb to HSP60, dilution 1:10,000, in green: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The single band at 24kDa corresponds to the UCHL1 protein, while the 60kDa band represents HSP60 protein. UCHL1 is detectable in CNS extracts and cells with neuronal properties but not in HeLa, NIH-3T3 and other non-neuronal cells.")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58874] - Analysis of SH-SY5Y cells stained with rabbit pAb to UCHL1, NB110-58874, dilution 1:1,000 in green, and costained with mouse mAb to fibrillarin, dilution 1:1,000 in red. Blue is DAPI staining of nuclear DNA. The UCHL1 antibody produces strong staining of the cellular cytoplasm of these cells which share many properties with neurons, while the fibrilarin antibody specifically labels nucleoli.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58874-img0005.jpg)
![Western Blot: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Blot of whole bovine brain extract stained with RPCA-UCHL1 showing a strong and clean band at about 24kDa.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NB110-58874-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Shows rat mixed neuron/glial cultures stained with Rabbit anti-UCHL1 antibody (green). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58874-img0003.jpg)
![Simple Western: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Simple Western lane view shows a specific band for PGP9.5/UCHL-1 in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Simple-Western-NB110-58874-img0004.jpg)
![Immunohistochemistry-Paraffin: CACNB2 Antibody [NBP1-86680] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding CACNB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CACNB2-Antibody-Immunohistochemistry-Paraffin-NBP1-86680-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CACNB2 Antibody [NBP1-86680] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding CACNB2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CACNB2 Antibody [NBP1-86680] - Staining of human prostate shows weak to moderate membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/CACNB2-Antibody-Immunohistochemistry-Paraffin-NBP1-86680-img0009.jpg)
![Immunohistochemistry-Paraffin: CACNB2 Antibody [NBP1-86680] - Staining of human cerebellum shows moderate to strong membranous positivity in Purkinje cells.](http://images.novusbio.com/fullsize/CACNB2-Antibody-Immunohistochemistry-Paraffin-NBP1-86680-img0007.jpg)
![Immunohistochemistry-Paraffin: CACNB2 Antibody [NBP1-86680] - Staining of human cerebral cortex shows moderate to strong membranous positivity in neurons.](http://images.novusbio.com/fullsize/CACNB2-Antibody-Immunohistochemistry-Paraffin-NBP1-86680-img0008.jpg)
![Immunohistochemistry-Paraffin: CACNB2 Antibody [NBP1-86680] - Staining of human skeletal muscle shows very weak positivity in myocytes.](http://images.novusbio.com/fullsize/CACNB2-Antibody-Immunohistochemistry-Paraffin-NBP1-86680-img0010.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Analysis in human cerebral cortex and testis tissues using NBP1-88769 antibody. Corresponding SNAP25 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0024.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Analysis in human cerebral cortex and testis tissues using NBP1-88769 antibody. Corresponding SNAP25 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: SNAP25 Antibody [NBP1-88769] - Analysis in human cell lines U2OS and MCF-7 using anti-SNAP25 antibody. Corresponding SNAP25 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Western-Blot-NBP1-88769-img0017.jpg)
![Western Blot: SNAP25 Antibody [NBP1-88769] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Western-Blot-NBP1-88769-img0025.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0020.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human cerebellum shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0021.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0022.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0023.jpg)
![Western Blot: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Western-Blot-NBP2-00795-img0002.jpg "Western Blot: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.")
![Immunocytochemistry/Immunofluorescence: SNAP25 Antibody (4G5) [NBP2-00795] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunocytochemistry-Immunofluorescence-NBP2-00795-img0009.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Human liver tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0008.jpg)
![Flow Cytometry: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAP25 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Flow-Cytometry-NBP2-00795-img0001.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0003.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0004.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0005.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0006.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Human colon tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0007.jpg)