| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87394-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14045-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59789-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44488 | |||
| BSA Free | NBP2-52868-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44489 | |||
| BSA Free | NBP2-14783-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74117 | |||
![Immunocytochemistry/Immunofluorescence: RP5-1077B9.4 Antibody [NBP1-87394] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/RP5-1077B9.4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87394-img0006.jpg "Immunocytochemistry/Immunofluorescence: RP5-1077B9.4 Antibody [NBP1-87394] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: RP5-1077B9.4 Antibody [NBP1-87394] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/RP5-1077B9.4-Antibody-Immunohistochemistry-Paraffin-NBP1-87394-img0009.jpg)
![Immunohistochemistry-Paraffin: RP5-1077B9.4 Antibody [NBP1-87394] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/RP5-1077B9.4-Antibody-Immunohistochemistry-Paraffin-NBP1-87394-img0001.jpg)
![Immunohistochemistry-Paraffin: RP5-1077B9.4 Antibody [NBP1-87394] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/RP5-1077B9.4-Antibody-Immunohistochemistry-Paraffin-NBP1-87394-img0005.jpg)
![Immunohistochemistry-Paraffin: RP5-1077B9.4 Antibody [NBP1-87394] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/RP5-1077B9.4-Antibody-Immunohistochemistry-Paraffin-NBP1-87394-img0007.jpg)
![Immunohistochemistry-Paraffin: RP5-1077B9.4 Antibody [NBP1-87394] - Staining of human testis shows weak cytoplasmic positivity in spermatogonia.](http://images.novusbio.com/fullsize/RP5-1077B9.4-Antibody-Immunohistochemistry-Paraffin-NBP1-87394-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/GFR-alpha-1-GDNF-R-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14045-img0003.jpg "Immunocytochemistry/Immunofluorescence: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.")
![Immunohistochemistry-Paraffin: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Staining of human kidney shows weak granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/GFR-alpha-1-GDNF-R-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14045-img0008.jpg)
![Immunohistochemistry-Paraffin: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Staining of human smooth muscle shows strong granular cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/GFR-alpha-1-GDNF-R-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14045-img0004.jpg)
![Immunohistochemistry-Paraffin: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Staining of human prostate shows strong granular cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/GFR-alpha-1-GDNF-R-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14045-img0005.jpg)
![Immunohistochemistry-Paraffin: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Staining of human colon shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/GFR-alpha-1-GDNF-R-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14045-img0006.jpg)
![Immunohistochemistry-Paraffin: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/GFR-alpha-1-GDNF-R-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14045-img0007.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining in human cervix, uterine and liver tissues using NBP2-59789 antibody. Corresponding PROM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59789-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining in human cervix, uterine and liver tissues using NBP2-59789 antibody. Corresponding PROM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD133 Antibody [NBP2-59789] - Analysis in human cell line CACO-2 and human cell line PC-3.](http://images.novusbio.com/fullsize/CD133-Antibody-Western-Blot-NBP2-59789-img0005.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59789-img0011.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining of human breast shows moderate to strong membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59789-img0006.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining of human cervix shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59789-img0007.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59789-img0008.jpg)
![Western Blot: CD45 Antibody (CL0159) [NBP2-52868] - Western Blot analysis using CD45 antibody (CL0159) [NBP2-52868]. Lane 1: Marker [kDa], Lane 2: Human tonsil tissue lysate. Observed molecular weight ~200 kDa.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Western-Blot-NBP2-52868-img0006.jpg "Western Blot: CD45 Antibody (CL0159) [NBP2-52868] - Western Blot analysis using CD45 antibody (CL0159) [NBP2-52868]. Lane 1: Marker [kDa], Lane 2: Human tonsil tissue lysate. Observed molecular weight ~200 kDa.")
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0159) [NBP2-52868] - Staining of human tonsil with CD45 antibody (CL0159) [NBP2-52868] shows strong membranous immunoreactivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Immunohistochemistry-Paraffin-NBP2-52868-img0005.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0159) [NBP2-52868] - Staining of human cortex using CD45 antibody (CL0159) [NBP2-52868] shows strong immunoreactivity in microglia.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Immunohistochemistry-NBP2-52868-img0001.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0159) [NBP2-52868] - Staining of human liver with CD45 antibody (CL0159) [NBP2-52868] shows strong positivity in Kupffer cells.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Immunohistochemistry-Paraffin-NBP2-52868-img0002.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0159) [NBP2-52868] - Staining of human lung with CD45 antibody (CL0159) [NBP2-52868] shows strong membranous positivity in macrophages.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Immunohistochemistry-Paraffin-NBP2-52868-img0003.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0159) [NBP2-52868] - Staining of human small intestine with CD45 antibody (CL0159) [NBP2-52868] shows strong immunoreactivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Immunohistochemistry-Paraffin-NBP2-52868-img0004.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Analysis in human tonsil and skeletal muscle tissues using NBP2-14783 antibody. Corresponding PTPRC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-14783_mouse-monoclonal-cd45-antibody-cl0160-immunohistochemistry-paraffin-39202411351..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Analysis in human tonsil and skeletal muscle tissues using NBP2-14783 antibody. Corresponding PTPRC RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD45 Antibody (CL0160) [NBP2-14783] - Western Blot analysis using CD45 antibody (CL0160) [NBP2-14783]. Lane 1: Marker [kDa]. Lane 2: Human cell line Jurkat. Lane 3: Human cell line MCF-7. Observed molecular weight ~230 kDa.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0160-Western-Blot-NBP2-14783-img0007.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] - Staining of human tonsil using CD45 antibody (CL0160) [NBP2-14783] shows strong positivity in a majority of lymphoid cells](http://images.novusbio.com/fullsize/CD45-Antibody-CL0160-Immunohistochemistry-Paraffin-NBP2-14783-img0005.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Staining of human lymph node shows moderate to strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/antibody/nbp2-14783_mouse-monoclonal-cd45-antibody-cl0160-immunohistochemistry-paraffin-39202411445..jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Staining of human duodenum shows moderate membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/antibody/nbp2-14783_mouse-monoclonal-cd45-antibody-cl0160-immunohistochemistry-paraffin-39202411549..jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-14783_mouse-monoclonal-cd45-antibody-cl0160-immunohistochemistry-paraffin-39202411643..jpg)
![Western Blot: Serpin E2/PN1 Antibody (2C9) [NBP2-01984] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINE2.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-2C9-Western-Blot-NBP2-01984-img0015.jpg "Western Blot: Serpin E2/PN1 Antibody (2C9) [NBP2-01984] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINE2.")
![Immunohistochemistry-Paraffin: Serpin E2/PN1 Antibody (2C9) [NBP2-01984] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SERPINE2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-2C9-Immunohistochemistry-Paraffin-NBP2-01984-img0014.jpg)
![Flow Cytometry: Serpin E2/PN1 Antibody (2C9) [NBP2-01984] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SERPINE2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-2C9-Flow-Cytometry-NBP2-01984-img0011.jpg)
![Immunohistochemistry-Paraffin: Serpin E2/PN1 Antibody (2C9) [NBP2-01984] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SERPINE2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-2C9-Immunohistochemistry-Paraffin-NBP2-01984-img0012.jpg)
![Immunohistochemistry-Paraffin: Serpin E2/PN1 Antibody (2C9) [NBP2-01984] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SERPINE2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-2C9-Immunohistochemistry-Paraffin-NBP2-01984-img0013.jpg)
![Western Blot: IGHMBP2 Antibody [NBP1-68921] - Mouse Heart.](http://images.novusbio.com/fullsize/IGHMBP2-Antibody-Western-Blot-NBP1-68921-img0001.jpg "Western Blot: IGHMBP2 Antibody [NBP1-68921] - Mouse Heart.")
![Immunocytochemistry/Immunofluorescence: IGHMBP2 Antibody [NBP1-68921] - IGHMBP2 in HEK293 cells. Antibody at 1:50. Image from verified customer review.](http://images.novusbio.com/fullsize/IGHMBP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-68921-img0002.jpg)
![Western Blot: Serpin E2/PN1 Antibody (OTI2C9) - Azide and BSA Free [NBP2-74117] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINE2.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-OTI2C9-Azide-and-BSA-Free-Western-Blot-NBP2-74117-img0001.jpg "Western Blot: Serpin E2/PN1 Antibody (OTI2C9) - Azide and BSA Free [NBP2-74117] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINE2.")
![Immunohistochemistry: Serpin E2/PN1 Antibody (OTI2C9) - Azide and BSA Free [NBP2-74117] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SERPINE2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-OTI2C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74117-img0004.jpg)
![Flow Cytometry: Serpin E2/PN1 Antibody (OTI2C9) - Azide and BSA Free [NBP2-74117] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SERPINE2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-OTI2C9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74117-img0002.jpg)
![Immunohistochemistry: Serpin E2/PN1 Antibody (OTI2C9) - Azide and BSA Free [NBP2-74117] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SERPINE2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-OTI2C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74117-img0003.jpg)
![Immunohistochemistry: Serpin E2/PN1 Antibody (OTI2C9) - Azide and BSA Free [NBP2-74117] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SERPINE2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-OTI2C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74117-img0005.jpg)
![Western Blot: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-OTI3G1-Azide-and-BSA-Free-Western-Blot-NBP2-70334-img0001.jpg "Western Blot: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2.")
![Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-OTI3G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70334-img0004.jpg)
![Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-OTI3G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70334-img0002.jpg)
![Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-OTI3G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70334-img0003.jpg)
![Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-OTI3G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70334-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204] - Staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red). Cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/AHNAK-Antibody-EM-09-Immunocytochemistry-Immunofluorescence-NBP2-62204-img0002.jpg "Immunocytochemistry/Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204] - Staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red). Cell nuclei stained with DAPI (blue).")
![Immunohistochemistry-Frozen: AHNAK Antibody (EM-09) [NBP2-62204] - Staining (frozen sections) of murine tongue by anti-AHNAK1 antibody (EM-09; red). Actin filaments were decorated by phalloidin (green), cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/AHNAK-Antibody-EM-09-Immunohistochemistry-Frozen-NBP2-62204-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204] - Staining of AHNAK1 in human primary fibroblasts using anti-AHNAK1 (EM-09; green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/AHNAK-Antibody-EM-09-Immunocytochemistry-Immunofluorescence-NBP2-62204-img0001.jpg)
![Western Blot: xCT Antibody (A7C6-R) [NBP3-33104] - Western blot analysis of xCT on different lysates with Mouse anti-xCT antibody (NBP3-33104) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-288202414191525.jpg "Western Blot: xCT Antibody (A7C6-R) [NBP3-33104] - Western blot analysis of xCT on different lysates with Mouse anti-xCT antibody (NBP3-33104) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: xCT Antibody (A7C6-R) [NBP3-33104] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-xCT antibody (NBP3-33104) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33104) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-298202413585586.jpg)
![Immunocytochemistry/ Immunofluorescence: xCT Antibody (A7C6-R) [NBP3-33104] - Immunocytochemistry analysis of HT-29 cells labeling xCT with Mouse anti-xCT antibody (NBP3-33104) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-xCT antibody (NBP3-33104) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-2920241114813.jpg)
![Flow Cytometry: xCT Antibody (A7C6-R) [NBP3-33104] - Flow cytometric analysis of A549 cells labeling xCT. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-33104, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-29202416372152.jpg)