| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70187 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87915-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38391-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38390-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-46852SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71049 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73588 | |||
![Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-western-blot-2942025163536..jpg "<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-western-blot-294202516917..jpg)
![Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-western-blot-2942025161213..jpg)
![Immunocytochemistry/ Immunofluorescence: Rabbit Tau mAb (HL2802) [NBP3-45161] - Tau antibody [HL2802] detects Tau protein at cytoskeleton by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tau stained by Tau antibody [HL2802] diluted at 1:250.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-immunocytochemistry-immunofluorescence-2942025161556..jpg)
![Immunohistochemistry: Rabbit Tau mAb (HL2802) [NBP3-45161] - Tau antibody [HL2802] detects Tau protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Tau stained by Tau antibody [HL2802] diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-immunohistochemistry-paraffin-2942025161950..jpg)
![Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-western-blot-2942025162224..jpg)
![Tau antibody [HL2802] detects Tau protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse eye.Green: Tau stained by Tau antibody [HL2802] (NBP3-45161) diluted at 1:250.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-32618_rabbit-polyclonal-protein-phosphatase-1-beta-antibody-immunohistochemistry-frozen-141120251855420.jpg)
![Western Blot: APP Antibody (OTI7G9) [NBP2-02358] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Amyloid Precursor Protein (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Amyloid Precursor Protein.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Western-Blot-NBP2-02358-img0002.jpg "Western Blot: APP Antibody (OTI7G9) [NBP2-02358] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Amyloid Precursor Protein (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Amyloid Precursor Protein.")
![Immunocytochemistry/Immunofluorescence: APP Antibody (OTI7G9) [NBP2-02358] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Amyloid Precursor Protein.](http://images.novusbio.com/fullsize/APP-Antibody-7G9-Immunocytochemistry-Immunofluorescence-NBP2-02358-img0009.jpg)
![Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded prostate tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunohistochemistry-Paraffin-NBP2-02358-img0007.jpg)
![Flow Cytometry: APP Antibody (OTI7G9) [NBP2-02358] - HEK293T cells transfected with either pCMV6-ENTRY Amyloid Precursor Protein.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Amyloid Precursor Protein mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Flow-Cytometry-NBP2-02358-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: APP Antibody (OTI7G9) [NBP2-02358] - Staining of HepG2 cells using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunocytochemistry-Immunofluorescence-NBP2-02358-img0003.jpg)
![Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded colon tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunohistochemistry-Paraffin-NBP2-02358-img0004.jpg)
![Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded Kidney tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunohistochemistry-Paraffin-NBP2-02358-img0005.jpg)
![Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded liver tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunohistochemistry-Paraffin-NBP2-02358-img0006.jpg)
![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Western-Blot-NBP2-54787-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate")
![Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde made from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54787-img0006.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to when 100 uM of active alpha Synuclein monomer (NBP2-54788) is combined with 10 nM of control alpha Synuclein aggregate (NBP2-54787), or 100 uM of control alpha Synuclein monomer (NBP2-54786) is combined with 10 nM of control alpha Synuclein aggregate (NBP2-54787). Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-In-vitro-assay-NBP2-54787-img0007.jpg)
![Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) (Catalog No. NBP2-54787) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Product-Image-NBP2-54787-img0009.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - TEM of Type 2 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54787)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Electron-Microscopy-NBP2-54787-img0008.jpg)
![Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.](http://images.novusbio.com/fullsize/Abeta-40-Antibody-Western-Blot-NBP1-44047-img0002.jpg "Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.")
![Immunocytochemistry/Immunofluorescence: Mena Antibody [NBP1-87915] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & focal adhesion sites. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87915-img0006.jpg "Immunocytochemistry/Immunofluorescence: Mena Antibody [NBP1-87915] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & focal adhesion sites. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Analysis in human testis and liver tissues. Corresponding ENAH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human endometrium, liver, pancreas and tonsil using Anti-AGXT antibody (A) NBP1-87915 shows similar protein distribution across tissues to independent antibody NBP1-87914 (B).](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0021.jpg)
![Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human prostate shows moderate to strong cytoplasmic and membranous positivity in glandular cells and smooth muscle cells.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0023.jpg)
![Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human Fallopian tube shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0018.jpg)
![Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human liver shows low positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0019.jpg)
![Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0020.jpg)
![Western Blot: HIP1 Related Antibody [NBP2-38391] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Western-Blot-NBP2-38391-img0003.jpg "Western Blot: HIP1 Related Antibody [NBP2-38391] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: HIP1 Related Antibody [NBP2-38391] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38391-img0004.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38391] - Staining of human kidney.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38391-img0008.jpg)
![Immunohistochemistry: HIP1 Related Antibody [NBP2-38391] - Staining of human kidney shows strong membranous and cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-NBP2-38391-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38391] - Staining of human cerebral cortex, colon, kidney and testis using Anti-HIP1R antibody NBP2-38391 (A) shows similar protein distribution across tissues to independent antibody NBP2-38390 (B).](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38391-img0005.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38391] - Staining of human testis.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38391-img0006.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38391] - Staining of human colon.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38391-img0007.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38391] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38391-img0009.jpg)
![Western Blot: HIP1 Related Antibody [NBP2-38390] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Western-Blot-NBP2-38390-img0003.jpg "Western Blot: HIP1 Related Antibody [NBP2-38390] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: HIP1 Related Antibody [NBP2-38390] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & vesicles.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38390-img0004.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38390] - Staining of human testis.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38390-img0008.jpg)
![Immunohistochemistry: HIP1 Related Antibody [NBP2-38390] - Staining of human kidney shows strong membranous and cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-NBP2-38390-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38390] - Staining of human cerebral cortex, colon, kidney and testis using Anti-HIP1R antibody NBP2-38390 (A) shows similar protein distribution across tissues to independent antibody NBP2-38391 (B).](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38390-img0005.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38390] - Staining of human colon.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38390-img0006.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38390] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38390-img0007.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38390] - Staining of human kidney.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38390-img0009.jpg)
![Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Western-Blot-NB300-749-img0007.jpg "Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).")
![Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 11) [NB300-749] - Mouse fibroblasts.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunocytochemistry-Immunofluorescence-NB300-749-img0004.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunohistochemistry-Paraffin-NB300-749-img0006.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunohistochemistry-Paraffin-NB300-749-img0005.jpg)
![Western Blot: RCAN1 Antibody [NBP1-46852] - Western blot analysis of human skeletal muscle tissue (A) using RCAN1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP1-46852-img0006.jpg "Western Blot: RCAN1 Antibody [NBP1-46852] - Western blot analysis of human skeletal muscle tissue (A) using RCAN1 antibody at 2 ug/ml.")
![Immunohistochemistry: RCAN1 Antibody [NBP1-46852] - Immunohistochemical analysis of RCAN1 in human fetal brain.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Immunohistochemistry-NBP1-46852-img0003.jpg)
![Western Blot: RCAN1 Antibody [NBP1-46852] - Analysis of RCAN1 in human fetal brain.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP1-46852-img0004.jpg)
![Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-3042025124758..jpg "<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: Rabbit Tau mAb (HL2736) [NBP3-45160] - Tau antibody [HL2736] detects Tau protein by immunofluorescent analysis.Sample: DIV11 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tau stained by Tau antibody [HL2736] diluted at 1:250.Red: MAP2, a microtubule marker, stained by MAP2 antibody [HM-2] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-immunohistochemistry-3042025123354..jpg)
![Immunocytochemistry/ Immunofluorescence: Rabbit Tau mAb (HL2736) [NBP3-45160] - Tau antibody [HL2736] detects Tau protein at cytoskeleton by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tau stained by Tau antibody [HL2736] diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-immunocytochemistry-immunofluorescence-3042025125627..jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Untreated (–) and treated (+) mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-3042025125836..jpg)
![Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-304202513045..jpg)
![Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-304202513345..jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Untreated (–) and treated (+) SK-N-SH whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-304202513540..jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-3042025132854..jpg)
![<b>Orthogonal Strategies Validation. </b>Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] (NBP3-45160) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-141120251831211.jpg)
![Tau antibody [HL2736] detects Tau protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse eye.Green: Tau stained by Tau antibody [HL2736] (NBP3-45160) diluted at 1:250.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-immunohistochemistry-frozen-14112025185543.jpg)
![Western Blot: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRS1.](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Western-Blot-NBP2-45435-img0018.jpg "Western Blot: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRS1.")
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0017.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0001.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0002.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0003.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0004.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0005.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0006.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0007.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0008.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0009.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0010.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0011.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0012.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0013.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0014.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0015.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0016.jpg)
![Western Blot: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PPT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/PPT1-Antibody-1F10-Western-Blot-NBP2-45388-img0007.jpg "Western Blot: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PPT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PPT1-Antibody-1F10-Immunohistochemistry-Paraffin-NBP2-45388-img0012.jpg)
![Western Blot: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPT1.](http://images.novusbio.com/fullsize/PPT1-Antibody-1F10-Western-Blot-NBP2-45388-img0006.jpg)
![Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PPT1-Antibody-1F10-Immunohistochemistry-Paraffin-NBP2-45388-img0008.jpg)
![Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PPT1-Antibody-1F10-Immunohistochemistry-Paraffin-NBP2-45388-img0009.jpg)
![Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PPT1-Antibody-1F10-Immunohistochemistry-Paraffin-NBP2-45388-img0010.jpg)
![Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PPT1-Antibody-1F10-Immunohistochemistry-Paraffin-NBP2-45388-img0011.jpg)