| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08936 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38323-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43924 | |||
| BSA Free | NBP2-52867-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37888-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82541-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45337 | |||
| Azide and BSA Free | NBP2-34716-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89820-25ul | |||
![Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Western-Blot-NBP3-07191-img0005.jpg "Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human tonsil stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0003.jpg)
![Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human A375 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Western-Blot-NBP3-07191-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human melanoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0002.jpg)
![Western Blot: SUMO1 Antibody (6F8B6) [NBP3-15676] - Western blot analysis of extracts of various cell lines, using SUMO1 antibody (NBP3-15676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/SUMO1-Antibody-6F8B6-Western-Blot-NBP3-15676-img0006.jpg "Western Blot: SUMO1 Antibody (6F8B6) [NBP3-15676] - Western blot analysis of extracts of various cell lines, using SUMO1 antibody (NBP3-15676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: SUMO1 Antibody (6F8B6) [NBP3-15676] - Western blot analysis of extracts of various cell lines, using SUMO1 antibody (NBP3-15676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/SUMO1-Antibody-6F8B6-Western-Blot-NBP3-15676-img0005.jpg)
![Western Blot: SUMO1 Antibody (6F8B6) [NBP3-15676] - Western blot analysis of extracts of various cell lines, using SUMO1 antibody (NBP3-15676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/SUMO1-Antibody-6F8B6-Western-Blot-NBP3-15676-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: SUMO1 Antibody (6F8B6) [SUMO1] - Confocal imaging of NIH/3T3 cells using [KO Validated] SUMO1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15676_rabbit-sumo1-mab-6f8b6-5220251733572.jpg)
![Immunohistochemistry: SUMO1 Antibody (6F8B6) [SUMO1] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KO Validated] SUMO1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15676_rabbit-sumo1-mab-6f8b6-5220251734543.jpg)
![Immunohistochemistry: SUMO1 Antibody (6F8B6) [SUMO1] - Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using [KO Validated] SUMO1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15676_rabbit-sumo1-mab-6f8b6-5220251734513.jpg)
![Immunohistochemistry: SUMO1 Antibody (6F8B6) [SUMO1] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KO Validated] SUMO1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15676_rabbit-sumo1-mab-6f8b6-52202517315566.jpg)
![Immunohistochemistry: SUMO1 Antibody (6F8B6) [SUMO1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KO Validated] SUMO1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15676_rabbit-sumo1-mab-6f8b6-5220251733570.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SUMO1 Antibody (6F8B6) [SUMO1] - Western blot analysis of lysates from wild type(WT) and SUMO1 knockout (KO) 293T cells, using [KO Validated] SUMO1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15676_rabbit-sumo1-mab-6f8b6-52202517361210.jpg)
![Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024158265.jpg "Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds")
![Immunohistochemistry-Frozen: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094).The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33094, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024159390.jpg)
![Immunohistochemistry: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33094) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-298202414161822.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunocytochemistry analysis of C6 cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202410234261.jpg)
![Flow Cytometry: Vimentin Antibody (SC60-05) [NBP3-33094] - Flow cytometric analysis of HeLa (positive, red) and MCF7 (negative, green) cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33094) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HeLa black, MCF7 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202416453228.jpg)
![Western Blot: Vimentin Antibody (SC60-05) [NBP3-33094] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/20,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HEK-293 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: C2C12 cell lysate (10 µg/Lane) Lane 5: RAW264.7 cell lysate (10 µg/Lane) Lane 6: C6 cell lysate (10 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-3920248453527.jpg)
![Western Blot: Rpn2 Antibody [NBP2-38323] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Rpn2-Antibody-Western-Blot-NBP2-38323-img0003.jpg "Western Blot: Rpn2 Antibody [NBP2-38323] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: Rpn2 Antibody [NBP2-38323] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & actin filaments.](http://images.novusbio.com/fullsize/Rpn2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38323-img0004.jpg)
![Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human liver.](http://images.novusbio.com/fullsize/Rpn2-Antibody-Immunohistochemistry-Paraffin-NBP2-38323-img0008.jpg)
![Immunohistochemistry: Rpn2 Antibody [NBP2-38323] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/Rpn2-Antibody-Immunohistochemistry-NBP2-38323-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human cerebral cortex, kidney, liver and testis using Anti-PSMD1 antibody NBP2-38323 (A) shows similar protein distribution across tissues to independent antibody NBP2-38324 (B).](http://images.novusbio.com/fullsize/Rpn2-Antibody-Immunohistochemistry-Paraffin-NBP2-38323-img0005.jpg)
![Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human testis.](http://images.novusbio.com/fullsize/Rpn2-Antibody-Immunohistochemistry-Paraffin-NBP2-38323-img0006.jpg)
![Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human kidney.](http://images.novusbio.com/fullsize/Rpn2-Antibody-Immunohistochemistry-Paraffin-NBP2-38323-img0007.jpg)
![Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Rpn2-Antibody-Immunohistochemistry-Paraffin-NBP2-38323-img0009.jpg)
![Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-52867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Knockout-Validated-NBP2-52867-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-52867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Analysis in human cell lines U-251MG and MCF-7 using Anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Western-Blot-NBP2-52867-img0010.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human skin shows distinct positivity in fibroblasts.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0005.jpg)
![Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Lane 1: Marker [kDa] Lane 2: Human tonsil tissue lysate](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Western-Blot-NBP2-52867-img0006.jpg)
![Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear fraction , Lane 5: Human cell line HeLa chromatin fraction , Lane 6: Human cell line HeLa cytos](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Western-Blot-NBP2-52867-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human cerebellum shows immunoreactivity in the endothelial cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human kidney shows strong immunoreactivity in renal glomeruli but not tubuli cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human prostate shows strong immunoreactivity in the connective tissue.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human rectum shows strong positivity in mesenchymal and lymphoid cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0004.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-37888] - Analysis in control (vector only transfected HEK293T lysate) and PSMD4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Western-Blot-NBP2-37888-img0006.jpg "Western Blot: S5a/Angiocidin Antibody [NBP2-37888] - Analysis in control (vector only transfected HEK293T lysate) and PSMD4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: S5a/Angiocidin Antibody [NBP2-37888] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-37888-img0005.jpg)
![Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human liver.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP2-37888-img0010.jpg)
![Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP2-37888-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human cerebral cortex, liver, lymph node and testis using Anti-PSMD4 antibody NBP2-37888 (A) shows similar protein distribution across tissues to independent antibody NBP1-90821 (B).](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP2-37888-img0007.jpg)
![Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP2-37888-img0008.jpg)
![Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human testis.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP2-37888-img0009.jpg)
![Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP2-37888-img0011.jpg)
![Immunohistochemistry-Paraffin: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541] - Staining in human epididymis and testis tissues using anti-PNP antibody. Corresponding PNP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Purine-Nucleoside-Phosphorylase-PNP-Antibody-Immunohistochemistry-Paraffin-NBP1-82541-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541] - Staining in human epididymis and testis tissues using anti-PNP antibody. Corresponding PNP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541] - Analysis in human cell lines HEK293 and SK-MEL-30 using Anti-PNP antibody. Corresponding PNP RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/Purine-Nucleoside-Phosphorylase-PNP-Antibody-Western-Blot-NBP1-82541-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541] - Staining of human cell line RPTEC TERT1 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Purine-Nucleoside-Phosphorylase-PNP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82541-img0022.jpg)
![Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/Purine-Nucleoside-Phosphorylase-PNP-Antibody-Western-Blot-NBP1-82541-img0012.jpg)
![Immunohistochemistry-Paraffin: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize/Purine-Nucleoside-Phosphorylase-PNP-Antibody-Immunohistochemistry-Paraffin-NBP1-82541-img0018.jpg)
![Immunohistochemistry-Paraffin: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541] - Staining of human testis shows low expression as expected.](http://images.novusbio.com/fullsize/Purine-Nucleoside-Phosphorylase-PNP-Antibody-Immunohistochemistry-Paraffin-NBP1-82541-img0019.jpg)
![Western Blot: SUMO1 Antibody (SM1/495) [NBP2-32901] - Analysis using the Azide and BSA Free version of NBP2-32901. Detection of SUMO1 in human HeLa Cell lysate.](http://images.novusbio.com/fullsize/SUMO1-Antibody-SM1-495-Western-Blot-NBP2-32901-img0004.jpg "Western Blot: SUMO1 Antibody (SM1/495) [NBP2-32901] - Analysis using the Azide and BSA Free version of NBP2-32901. Detection of SUMO1 in human HeLa Cell lysate.")
![Immunocytochemistry/Immunofluorescence: SUMO1 Antibody (SM1/495) [NBP2-32901] - Paraformaldehyde-fixed HepG2 cells stained with SUMO1 Antibody (SM1/495)followed by goat anti-Mouse IgG-CF488 (Green). Cell membranes are stained with Phalloidin (red).](http://images.novusbio.com/fullsize/SUMO1-Antibody-SM1-495-Immunocytochemistry-Immunofluorescence-NBP2-32901-img0007.jpg)
![Immunohistochemistry-Paraffin: SUMO1 Antibody (SM1/495) [NBP2-32901] - Formalin-fixed, paraffin-embedded human tonsil stained with SUMO-1 MAb (SM1/495)](http://images.novusbio.com/fullsize/SUMO1-Antibody-SM1-495-Immunohistochemistry-Paraffin-NBP2-32901-img0001.jpg)
![Flow Cytometry: SUMO1 Antibody (SM1/495) [NBP2-32901] - Flow Cytometric Analysis of paraformaldehyde-fixed HepG2 cells using SUMO1 Antibody (SM1/495) followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/SUMO1-Antibody-SM1-495-Flow-Cytometry-NBP2-32901-img0006.jpg)
![Western Blot: USP7 Antibody [NBP2-24641] - analysis of USP7 in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 0 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/USP7-Antibody-Western-Blot-NBP2-24641-img0002.jpg "Western Blot: USP7 Antibody [NBP2-24641] - analysis of USP7 in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 0 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: USP7 Antibody [NBP2-24641] - analysis of USP7 in formalin-fixed, paraffin-embedded human stomach tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/USP7-Antibody-Immunohistochemistry-Paraffin-NBP2-24641-img0001.jpg)
![Western Blot: BIRC6 Antibody [NB300-264] - Detection of Human and Mouse BIRC6 by Western Blot. Samples: Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIRC6 antibody NB300-264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Western-Blot-NB300-264-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: BIRC6 Antibody [NB300-264] - Detection of Human and Mouse BIRC6 by Western Blot. Samples: Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIRC6 antibody NB300-264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: BIRC6 Antibody [NB300-264] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BIRC6/Apollon used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Coutnerstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-Paraffin-NB300-264-img0008.jpg)
![Immunoprecipitation: BIRC6 Antibody [NB300-264] - Detection of human BIRC6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BIRC6 antibody NB300-264 (lot 2) used for IP at 6 ug per reaction. BIRC6 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated BIRC6, NB300-264 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunoprecipitation-NB300-264-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Frequenin Antibody [NBP1-89820] - Staining of human cell line U-2 OS shows localization to plasma membrane and vesicles.](http://images.novusbio.com/fullsize/Frequenin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89820-img0013.jpg "Immunocytochemistry/Immunofluorescence: Frequenin Antibody [NBP1-89820] - Staining of human cell line U-2 OS shows localization to plasma membrane and vesicles.")
![Immunohistochemistry-Paraffin: Frequenin Antibody [NBP1-89820] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Frequenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89820-img0010.jpg)
![Immunohistochemistry-Paraffin: Frequenin Antibody [NBP1-89820] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Frequenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89820-img0011.jpg)
![Western Blot: Frequenin Antibody [NBP1-89820] - Analysis in control (vector only transfected HEK293T lysate) and NCS1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Frequenin-Antibody-Western-Blot-NBP1-89820-img0014.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp1-89820_rabbit-polyclonal-frequenin-antibody-15520258574147.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp1-89820_rabbit-polyclonal-frequenin-antibody-15520258273033.jpg)
![Immunohistochemistry-Frozen: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: HCN1 protein stained by HCN1 antibody (NBP3-13014) diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Frozen-NBP3-13014-img0008.jpg "Immunohistochemistry-Frozen: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: HCN1 protein stained by HCN1 antibody (NBP3-13014) diluted at 1:250. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein in mouse retina by immunohistochemical analysis. Sample: Paraffin-embedded mouse retina. HCN1 antibody (NBP3-13014) diluted at 1:800. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-13014-img0002.jpg)
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. HCN1 antibody (NBP3-13014) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-13014-img0003.jpg)
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HCN1 antibody (NBP3-13014) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-13014-img0004.jpg)
![Immunohistochemistry-Frozen: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: HCN1 protein stained by HCN1 antibody (NBP3-13014) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Frozen-NBP3-13014-img0005.jpg)
![HCN1 antibody detects HCN1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HCN1 stained by HCN1 antibody (NBP3-13014) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13014_rabbit-hcn1-pab-immunocytochemistry-immunofluorescence-261120251818921.jpg)