| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93832-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB300-174-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-82375SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92686SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-153SS | |||
| Azide and BSA Free | NBP2-80870 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59376-25ug | |||
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249432833.jpg "Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249415999.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-212024943553.jpg)
![Western Blot: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - Various extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249374992.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249374949.jpg)
![Western Blot: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249432886.jpg)
![Immunocytochemistry/Immunofluorescence: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in ice-cold MeOH for 5 min. Green: MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249342758.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded A375 xenograft . MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249362712.jpg)
![Frozen-sectioned mouse cerebellum.Green: MC1 Receptor stained by MC1 Receptor antibody [HL1471] diluted at 1:100.Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-immunohistochemistry-frozen-119202513498.png)
![<b>Independent Antibodies Validation. </b>Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1471] (NBP3-25558) diluted at 1:50000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-western-blot-141120251843244.jpg)
![<b>Independent Antibodies Validation. </b>Various tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1471] (NBP3-25558) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-western-blot-1411202518433316.jpg)
![<b>Independent Antibodies Validation, Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Non-transfected (-) and transfected (+) unboiled A375 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1471] (NBP3-25558) diluted at 1:5000 and competitor's antibody (Highly Cited Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-western-blot-14112025183376.jpg)
![Western Blot: MT-ND3 Antibody [NBP2-93832] - Analysis of extracts of various cell lines, using MT-ND3 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MT-ND3-Antibody-Western-Blot-NBP2-93832-img0007.jpg "Western Blot: MT-ND3 Antibody [NBP2-93832] - Analysis of extracts of various cell lines, using MT-ND3 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: MT-ND3 Antibody [NBP2-93832] - Analysis of L929 cells using MT-ND3 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MT-ND3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93832-img0001.jpg)
![Immunohistochemistry-Paraffin: MT-ND3 Antibody [NBP2-93832] - Mouse kidney using MT-ND3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MT-ND3-Antibody-Immunohistochemistry-Paraffin-NBP2-93832-img0004.jpg)
![Immunohistochemistry-Paraffin: MT-ND3 Antibody [NBP2-93832] - Human appendix using MT-ND3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MT-ND3-Antibody-Immunohistochemistry-Paraffin-NBP2-93832-img0002.jpg)
![Immunohistochemistry-Paraffin: MT-ND3 Antibody [NBP2-93832] - Human colon carcinoma using MT-ND3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MT-ND3-Antibody-Immunohistochemistry-Paraffin-NBP2-93832-img0003.jpg)
![Immunoprecipitation: MT-ND3 Antibody [NBP2-93832] - Analysis of 200ug extracts of HeLa cells using 3ug MT-ND3 antibody. Western blot was performed from the immunoprecipitate using MT-ND3 antibody at a dilition of 1:500.](http://images.novusbio.com/fullsize/MT-ND3-Antibody-Immunoprecipitation-NBP2-93832-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: MT-ND3 Antibody - Azide and BSA Free [MT-ND3] - Immunofluorescence analysis of HepG2 cells using MT-ND3 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93832_rabbit-polyclonal-mt-nd3-antibody-52202516293969.jpg)
![Western Blot: ATP5A Antibody (6M3B8) [NBP3-15355] - Analysis of extracts of various cell lines, using ATP5A Rabbit mAb (NBP3-15355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ATP5A-Antibody-6M3B8-Western-Blot-NBP3-15355-img0006.jpg "Western Blot: ATP5A Antibody (6M3B8) [NBP3-15355] - Analysis of extracts of various cell lines, using ATP5A Rabbit mAb (NBP3-15355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Simple Western: ATP5A Antibody (6M3B8) [NBP3-15355] - ATP5A Antibodies (NBP3-15355), ProteinSimple Western Blot on Jess Instrument; 1, 0.5, and 0.2 microgram of human brain tissue lysate was tested with the antibodies diluted 20 times. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-simple-western-204202311391.jpg)
![Immunocytochemistry/Immunofluorescence: ATP5A Antibody (6M3B8) [NBP3-15355] - Confocal imaging of NIH/3T3 cells using ATP5A1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-immunocytochemistry-immunofluorescence-412202412213..png)
![Immunohistochemistry-Paraffin: ATP5A Antibody (6M3B8) [NBP3-15355] -Rat brain tissue using ATP5A1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-immunohistochemistry-paraffin-412202412333..png)
![Immunohistochemistry-Paraffin: ATP5A Antibody (6M3B8) [NBP3-15355] - Mouse brain tissue using ATP5A1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-immunohistochemistry-paraffin-412202412451..png)
![Immunocytochemistry/Immunofluorescence: ATP5A Antibody (6M3B8) [NBP3-15355] - Confocal imaging of HeLa cells using ATP5A1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-immunocytochemistry-immunofluorescence-412202412651..png)
![Immunohistochemistry: ATP5A Antibody (6M3B8) [ATP5A] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using ATP5A Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517223956.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5A Antibody (6M3B8) [ATP5A] - Confocal imaging of paraffin-embedded Mouse brain tissue using ATP5A Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-5220251718967.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5A Antibody (6M3B8) [ATP5A] - Confocal imaging of NIH/3T3 cells using ATP5A Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517251916.jpg)
![Immunohistochemistry: ATP5A Antibody (6M3B8) [ATP5A] - Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using ATP5A Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517235715.jpg)
![Immunohistochemistry: ATP5A Antibody (6M3B8) [ATP5A] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using ATP5A Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-522025172603.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5A Antibody (6M3B8) [ATP5A] - Confocal imaging of paraffin-embedded Rat brain tissue using ATP5A Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517223923.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5A Antibody (6M3B8) [ATP5A] - Confocal imaging of HeLa cells using ATP5A Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517202976.jpg)
![Immunoprecipitation: ATP5A Antibody (6M3B8) [ATP5A] - Immunoprecipitation analysis of 600 ug extracts of Mouse heart using 3 ug ATP5A antibody . Western blot was performed from the immunoprecipitate using ATP5A antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517162373.jpg)
![Immunohistochemistry: ATP5A Antibody (6M3B8) [ATP5A] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ATP5A Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517152767.jpg)
![Immunohistochemistry: ATP5A Antibody (6M3B8) [ATP5A] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using ATP5A Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517235771.jpg)
![Immunohistochemistry: ATP5A Antibody (6M3B8) [ATP5A] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using ATP5A Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517202985.jpg)
![Western Blot: EWSR1 Antibody (9G3H1) [NBP3-16845] - Western blot analysis of extracts of various cell lines, using EWSR1 Rabbit mAb (NBP3-16845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/EWSR1-Antibody-9G3H1-Western-Blot-NBP3-16845-img0007.jpg "Western Blot: EWSR1 Antibody (9G3H1) [NBP3-16845] - Western blot analysis of extracts of various cell lines, using EWSR1 Rabbit mAb (NBP3-16845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunofluorescence analysis of U-2 OS cells using EWSR1 Rabbit mAb (NBP3-16845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/EWSR1-Antibody-9G3H1-Immunocytochemistry-Immunofluorescence-NBP3-16845-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunofluorescence analysis of C6 cells using EWSR1 Rabbit mAb (NBP3-16845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/EWSR1-Antibody-9G3H1-Immunocytochemistry-Immunofluorescence-NBP3-16845-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunofluorescence analysis of NIH-3T3 cells using EWSR1 Rabbit mAb (NBP3-16845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/EWSR1-Antibody-9G3H1-Immunocytochemistry-Immunofluorescence-NBP3-16845-img0005.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded human breast cancer tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-822025209823.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded rat colon tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-82202520982.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded mouse kidney tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-822025209884.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded mouse lung tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-82202520104012.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded mouse colon tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-8220252010463.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded human thyroid tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-8220252010470.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded rat spleen tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-8220252010450.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded human tonsil tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-822025209895.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded human breast tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-822025209877.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded mouse heart tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-822025208472.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded mouse liver tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-822025208433.jpg)
![Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla. The antibody is specific for the ~55 kDa DOPA decarboxylase protein.](http://images.novusbio.com/fullsize/Dopa-Decarboxylase-DDC-Antibody-Western-Blot-NB300-174-img0005.jpg "Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla. The antibody is specific for the ~55 kDa DOPA decarboxylase protein.")
![Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla showing specific immunolabeling of the ~55k DDC protein.](http://images.novusbio.com/fullsize/Dopa-Decarboxylase-DDC-Antibody-Western-Blot-NB300-174-img0004.jpg)
![Simple Western: Dopa Decarboxylase/DDC Antibody [NB300-174] - Simple Western lane view shows a specific band for DOPA Decarboxylase in 0.2 mg/ml of Mouse Cerebellum lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize/Dopa-Decarboxylase-DDC-Antibody-Simple-Western-NB300-174-img0003.jpg)
![Western Blot: Mucolipin 1 Antibody [NB110-82375] - Total protein from mouse brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Mucolipin 1 (NB110-82375) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Western-Blot-NB110-82375-img0005.jpg "Western Blot: Mucolipin 1 Antibody [NB110-82375] - Total protein from mouse brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Mucolipin 1 (NB110-82375) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Simple Western: Mucolipin 1 Antibody [NB110-82375] - Lane view shows a specific band for Mucolipin 1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Simple-Western-NB110-82375-img0004.jpg)
![Western Blot: EWSR1 Antibody (5H7) [NBP1-92686] - Image shows a specific band for EWSR1 (observed molecular weight ~95 kDa) in HEK293T and huh-7 lysate.](http://images.novusbio.com/fullsize/EWSR1-Antibody-5H7-Western-Blot-NBP1-92686-img0005.jpg "Western Blot: EWSR1 Antibody (5H7) [NBP1-92686] - Image shows a specific band for EWSR1 (observed molecular weight ~95 kDa) in HEK293T and huh-7 lysate.")
![Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (5H7) [NBP1-92686] - HeLa cell cultures stained with antibody NBP1-92686 (green) and chicken antibody to vimentin (NB300-223, red). Blue is a DNA stain, and it is clear that in these cells EWSR1 is localized along with the DNA in the nucleus.](http://images.novusbio.com/fullsize/EWSR1-Antibody-5H7-Immunocytochemistry-Immunofluorescence-NBP1-92686-img0003.jpg)
![Western Blot: EWSR1 Antibody (5H7) [NBP1-92686] - Analysis of different cell line lysates using EWSR1 antibody, dilution 1:1000 (Green): [1] protein standard (Red), [2] HeLa, [3] HEK293, [4] mouse NIH-3T3, [5] rat PC12, [6] equine NBL6 and [7] canine A72 cells. The strong band at ~80kDa corresponds to the EWS protein seen in all species tested.](http://images.novusbio.com/fullsize/EWSR1-Antibody-5H7-Western-Blot-NBP1-92686-img0004.jpg)
![Western Blot: KCNJ10 Antibody [NBP1-20149] - WB on mouse tissue lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:3000 incubated at 4C overnight.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Western-Blot-NBP1-20149-img0013.jpg "Western Blot: KCNJ10 Antibody [NBP1-20149] - WB on mouse tissue lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:3000 incubated at 4C overnight.")
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0012.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0002.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0005.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of mouse kidney. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer from following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0006.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat kidney. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0007.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat kidney. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0009.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat heart. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0010.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0011.jpg)
![Immunohistochemistry-Paraffin: mGluR6 Antibody [NLS4655] - Eye, Retina, Ganglion cells.](http://images.novusbio.com/fullsize/mGluR6-Antibody-Immunohistochemistry-Paraffin-NLS4655-img0010.jpg "Immunohistochemistry-Paraffin: mGluR6 Antibody [NLS4655] - Eye, Retina, Ganglion cells.")
![Immunohistochemistry-Paraffin: mGluR6 Antibody [NLS4655] - Analysis of anti-GRM6 / MGLUR6 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/mGluR6-Antibody-Immunohistochemistry-Paraffin-NLS4655-img0005.jpg)
![Immunohistochemistry-Paraffin: mGluR6 Antibody [NLS4655] - Human eye, retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/mGluR6-Antibody-Immunohistochemistry-Paraffin-NLS4655-img0006.jpg)
![Immunohistochemistry-Paraffin: mGluR6 Antibody [NLS4655] - Human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/mGluR6-Antibody-Immunohistochemistry-Paraffin-NLS4655-img0007.jpg)
![Immunohistochemistry-Paraffin: mGluR6 Antibody [NLS4655] - Human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/mGluR6-Antibody-Immunohistochemistry-Paraffin-NLS4655-img0008.jpg)
![Immunohistochemistry-Paraffin: mGluR6 Antibody [NLS4655] - Eye, Retina](http://images.novusbio.com/fullsize/mGluR6-Antibody-Immunohistochemistry-Paraffin-NLS4655-img0009.jpg)
![Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Western-Blot-NB300-153-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human brain using Neurofibromin 1 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of Neurofibromin 1was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Immunohistochemistry-Paraffin-NB300-153-img0005.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2A.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Western-Blot-NBP2-45602-img0008.jpg "Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2A.")
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HepG2 cells.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0003.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Carcinoma of Human liver tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0007.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: Human; HeLa: Human; HT29: Human; A549: Human; COS7: monkey; Jurkat: human; MDCK: Canine; PC12: Rat; MCF7: human)](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Western-Blot-NBP2-45602-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CDKN2A.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0002.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0004.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0005.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0006.jpg)
![Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Western Blot analysis of Rat Liver showing detection of ~180 kDa ATP7A protein using Mouse Anti-ATP7A Monoclonal Antibody, Clone S60-4 (NBP2-59376). Lane 1: MW Ladder. Lane 2: Rat Liver . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse ATP7A Monoclonal Antibody (NBP2-59376) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~180 kDa.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Western-Blot-NBP2-59376-img0005.jpg "Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Western Blot analysis of Rat Liver showing detection of ~180 kDa ATP7A protein using Mouse Anti-ATP7A Monoclonal Antibody, Clone S60-4 (NBP2-59376). Lane 1: MW Ladder. Lane 2: Rat Liver . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse ATP7A Monoclonal Antibody (NBP2-59376) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~180 kDa.")
![Immunocytochemistry/Immunofluorescence: ATP7A Antibody (S60-4) [NBP2-59376] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody, Clone S60-4 (NBP2-59376). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody (NBP2-59376) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Copper Transporting ATPase 1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Immunocytochemistry-Immunofluorescence-NBP2-59376-img0006.jpg)
![Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Analysis of Mouse Lung showing detection of >150 kDa Copper Transporting ATPase 1 protein using clone S60-4. 10 ug of sample was loaded and primary antibody was used at 1:1000. Predicted/Observed Size: ~160 kDa. Other band(s): 250 kDa.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Western-Blot-NBP2-59376-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7A Antibody (S60-4) [NBP2-59376] - Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum, Cytoplasm, Golgi Apparatus, Trans-Golgi Network Membrane, Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Immunocytochemistry-Immunofluorescence-NBP2-59376-img0002.jpg)