| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88661-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84199-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89196-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59057 | |||
| BSA Free | NBP2-59057-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77060-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02943-20ul | |||
![Western Blot: NeuroD1 Antibody [NBP1-88661] - Analysis in control (vector only transfected HEK293T lysate) and NEUROD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/NeuroD1-Antibody-Western-Blot-NBP1-88661-img0006.jpg "Western Blot: NeuroD1 Antibody [NBP1-88661] - Analysis in control (vector only transfected HEK293T lysate) and NEUROD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Western Blot: NeuroD1 Antibody [NBP1-88661] - Example of the positive control of the four key molecules by WB in SCLC cell lines. NeuroD1 was strongly expressed in H82 cells. Beta-actin served as an internal control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33202998/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-88661_rabbit-polyclonal-neurod1-antibody-20920231412573.jpg)
![Immunohistochemistry: NeuroD1 Antibody [NBP1-88661] - Example of the positive control of the four key molecules in xenotransplanted tumor tissues from the four cell lines in mice by IHC. NeuroD1 staining was found in the tumor cell nuclei of H82 cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33202998/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-88661_rabbit-polyclonal-neurod1-antibody-2092023181150.jpg)
![Immunohistochemistry: NeuroD1 Antibody [NBP1-88661] - IHC of the co-expression of key molecules other than ASCL1 in SCLC samples. Serial section immunostaining for NEUROD1 (A) & YAP1 (B): SCLC cell nuclei were positive for NEUROD1 (A), & the cytoplasm was positive for YAP1 (B). Serial section immunostaining for NEUROD1 (C) & POU2F31 (D): SCLC cell nuclei were positive for NEUROD1 (C), & the cytoplasm was positive for POU2F3 (D). The cytoplasm of SCLC cells was positive for YAP1 (E) & POU2F3 (F). Scale bar = 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33202998), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88661_rabbit-polyclonal-neurod1-antibody-310202415395953.jpg)
![Immunohistochemistry: NeuroD1 Antibody [NBP1-88661] - IHC of the co-expression of key molecules other than ASCL1 in SCLC samples. Serial section immunostaining for NEUROD1 (A) & YAP1 (B): SCLC cell nuclei were positive for NEUROD1 (A), & the cytoplasm was positive for YAP1 (B). Serial section immunostaining for NEUROD1 (C) & POU2F31 (D): SCLC cell nuclei were positive for NEUROD1 (C), & the cytoplasm was positive for POU2F3 (D). The cytoplasm of SCLC cells was positive for YAP1 (E) & POU2F3 (F). Scale bar = 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33202998), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88661_rabbit-polyclonal-neurod1-antibody-31020241534377.jpg)
![Immunohistochemistry: NeuroD1 Antibody [NBP1-88661] - Example of the positive control of four key molecules by WB & IHC in SCLC samples. (A) Example of the positive control of the four key molecules by WB in SCLC cell lines. ASCL1 was strongly expressed in H209 cells. NeuroD1 was strongly expressed in H82 cells. Pou2F3 was strongly expressed in H526 cells & weakly expressed in H82 & SBC3 cells. YAP1 was strongly expressed in SBC3 cells. beta -actin served as an internal control. (B) Example of the positive control of the four key molecules in xenotransplanted tumor tissues from the four cell lines in mice by IHC. ASCL1 staining was found in tumor cell nuclei of H209 cells. NeuroD1 staining was found in the tumor cell nuclei of H82 cells. Pou2F3 staining with a diffuse cytoplasmic pattern was found in H82, H526, & SBC3 cells. The Pou2F3 staining intensity was weak in H82 & SBC3 cells & strong in H526 cells. YAP1 was stained with a membranous pattern in SBC3 cells. Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33202998), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88661_rabbit-polyclonal-neurod1-antibody-310202416114786.jpg)
![Western Blot: NeuroD1 Antibody [NBP1-88661] - Example of the positive control of four key molecules by WB & IHC in SCLC samples. (A) Example of the positive control of the four key molecules by WB in SCLC cell lines. ASCL1 was strongly expressed in H209 cells. NeuroD1 was strongly expressed in H82 cells. Pou2F3 was strongly expressed in H526 cells & weakly expressed in H82 & SBC3 cells. YAP1 was strongly expressed in SBC3 cells. beta -actin served as an internal control. (B) Example of the positive control of the four key molecules in xenotransplanted tumor tissues from the four cell lines in mice by IHC. ASCL1 staining was found in tumor cell nuclei of H209 cells. NeuroD1 staining was found in the tumor cell nuclei of H82 cells. Pou2F3 staining with a diffuse cytoplasmic pattern was found in H82, H526, & SBC3 cells. The Pou2F3 staining intensity was weak in H82 & SBC3 cells & strong in H526 cells. YAP1 was stained with a membranous pattern in SBC3 cells. Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33202998), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88661_rabbit-polyclonal-neurod1-antibody-310202416114787.jpg)
![Western Blot: GBX2 Antibody [NBP2-43617] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/GBX2-Antibody-Western-Blot-NBP2-43617-img0006.jpg "Western Blot: GBX2 Antibody [NBP2-43617] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted at 1:1000.")
![Western Blot: GBX2 Antibody [NBP2-43617] - Analysis of IMR32 whole cell extracts and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/GBX2-Antibody-Western-Blot-NBP2-43617-img0001.jpg)
![Western Blot: GBX2 Antibody [NBP2-43617] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/GBX2-Antibody-Western-Blot-NBP2-43617-img0003.jpg)
![Western Blot: GBX2 Antibody [NBP2-43617] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/GBX2-Antibody-Western-Blot-NBP2-43617-img0004.jpg)
![Western Blot: GBX2 Antibody [NBP2-43617] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (NBP2-43617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43617_rabbit-polyclonal-gbx2-antibody-910202420104463.jpg)
![Western Blot: GBX2 Antibody [NBP2-43617] - GBX2 antibody detects GBX2 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (NBP2-43617) diluted by 1:1000.](http://images.novusbio.com/fullsize/nbp2-43617_rabbit-polyclonal-gbx2-antibody-91020242091476.jpg)
![Immunocytochemistry/Immunofluorescence: RAB3GAP2 Antibody [NBP1-84199] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RAB3GAP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84199-img0004.jpg "Immunocytochemistry/Immunofluorescence: RAB3GAP2 Antibody [NBP1-84199] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: RAB3GAP2 Antibody [NBP1-84199] - Staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/antibody/nbp1-84199_rabbit-polyclonal-rab3gap2-antibody-immunohistochemistry-paraffin-95202495355..jpg)
![Immunohistochemistry-Paraffin: RAB3GAP2 Antibody [NBP1-84199] -Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-84199_rabbit-polyclonal-rab3gap2-antibody-immunohistochemistry-paraffin-95202495454..jpg)
![Immunohistochemistry-Paraffin: RAB3GAP2 Antibody [NBP1-84199] -Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-84199_rabbit-polyclonal-rab3gap2-antibody-immunohistochemistry-paraffin-9520249562..jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0002.jpg "Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human testis with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0001.jpg)
![Western Blot: Synapsin II Antibody [NBP3-13445] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody (NBP3-13445) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Synapsin-II-Antibody-Western-Blot-NBP3-13445-img0002.jpg "Western Blot: Synapsin II Antibody [NBP3-13445] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody (NBP3-13445) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Synapsin II Antibody [NBP3-13445] - Rat brain. Synapsin II stained by Synapsin II antibody (NBP3-13445) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Synapsin-II-Antibody-Immunohistochemistry-Paraffin-NBP3-13445-img0005.jpg)
![Western Blot: Synapsin II Antibody [NBP3-13445] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody (NBP3-13445) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Synapsin-II-Antibody-Western-Blot-NBP3-13445-img0001.jpg)
![Immunohistochemistry-Paraffin: Synapsin II Antibody [NBP3-13445] - Mouse brain. Synapsin II stained by Synapsin II antibody (NBP3-13445) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Synapsin-II-Antibody-Immunohistochemistry-Paraffin-NBP3-13445-img0004.jpg)
![Synapsin II antibody detects Synapsin II protein at Synapse by immunofluorescent analysis.Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Synapsin II stained by Synapsin II antibody (NBP3-13445) diluted at 1:500.Red: Tau, a marker, stained by Tau antibody [GT287] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp3-13445_rabbit-synapsin-ii-pab-immunocytochemistry-immunofluorescence-2611202518283516.jpg)
![Western Blot: Sonic Hedgehog/Shh Antibody [NBP1-69270] - Lane A: Marker; Lane B HepG2 Cell Lysate. Antibody titration 1.0ug/ml. Gel concentration 12%](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-Western-Blot-NBP1-69270-img0005.jpg "Western Blot: Sonic Hedgehog/Shh Antibody [NBP1-69270] - Lane A: Marker; Lane B HepG2 Cell Lysate. Antibody titration 1.0ug/ml. Gel concentration 12%")
![Immunohistochemistry: Sonic Hedgehog/Shh Antibody [NBP1-69270] - Chicken embryos Primary Antibody: 1 : 1000 (NBP1-69270) anti -shh Secondary Antibody: 1 : 500 goat anti-rabbit HRP conjugated.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-Immunohistochemistry-NBP1-69270-img0009.jpg)
![Immunohistochemistry-Paraffin: Sonic Hedgehog/Shh Antibody [NBP1-69270] - Human Intestine cells with positive label: Epithelial cells of intestinal villus (indicated with arrows). Concentration 4.0-8.0 ug/ml](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-Immunohistochemistry-Paraffin-NBP1-69270-img0002.jpg)
![Immunohistochemistry: Sonic Hedgehog/Shh Antibody [NBP1-69270] - Human glioma cells. Primary Antibody Dilution :1:200 Secondary Antibody :Anti-rabbit-GFP. Secondary Antibody Dilution :1:500. Color/Signal Descriptions :1. SHH: Green 2. DAPI: Blue 3. Merge. Gene Name :SHH](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-Immunohistochemistry-NBP1-69270-img0007.jpg)
![Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-26820241525104.jpg "Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Western Blot: SOX2 Antibody (PO00-28) [NBP3-32972] - Western blot analysis of SOX2 on different lysates with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/2,000 dilution. Lane 1: NCCIT cell lysate Lane 2: F9 cell lysate Lane 3: HeLa cell lysate (negative) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32972) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-28820241249344.jpg)
![Immunohistochemistry: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-298202414161844.jpg)
![Immunocytochemistry/ Immunofluorescence: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunocytochemistry analysis of F9 cells labeling SOX2 with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-29202410334048.jpg)
![Western Blot: NOTUM Antibody [NBP1-77060] - Human kidney tissue lysate with NOTUM antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/NOTUM-Antibody-Western-Blot-NBP1-77060-img0001.jpg "Western Blot: NOTUM Antibody [NBP1-77060] - Human kidney tissue lysate with NOTUM antibody at (A) 0.5 and (B) 1 ug/mL.")
![Immunohistochemistry-Paraffin: NOTUM Antibody [NBP1-77060] - Human brain tissue with NOTUM antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/NOTUM-Antibody-Immunohistochemistry-Paraffin-NBP1-77060-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: NOTUM Antibody - BSA Free [NBP1-77060] - Immunofluorescence of NOTUM in Mouse Kidney cells with NOTUM antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77060_rabbit-polyclonal-notum-antibody-271120241928873.jpg)
![Western Blot: USP9x Antibody (7T0S2) [NBP3-16884] - Western blot analysis of extracts of various cell lines, using USP9x Rabbit mAb (NBP3-16884) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/USP9x-Antibody-7T0S2-Western-Blot-NBP3-16884-img0004.jpg "Western Blot: USP9x Antibody (7T0S2) [NBP3-16884] - Western blot analysis of extracts of various cell lines, using USP9x Rabbit mAb (NBP3-16884) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (7T0S2) [NBP3-16884] - Immunofluorescence analysis of NIH-3T3 cells using USP9x Rabbit mAb (NBP3-16884) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/USP9x-Antibody-7T0S2-Immunocytochemistry-Immunofluorescence-NBP3-16884-img0003.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (7T0S2) [NBP3-16884] - Immunohistochemistry of paraffin-embedded mouse testis using USP9x Rabbit mAb (NBP3-16884) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/USP9x-Antibody-7T0S2-Immunohistochemistry-Paraffin-NBP3-16884-img0002.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (7T0S2) [NBP3-16884] - Immunohistochemistry of paraffin-embedded rat testis using USP9x Rabbit mAb (NBP3-16884) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/USP9x-Antibody-7T0S2-Immunohistochemistry-Paraffin-NBP3-16884-img0001.jpg)
![Western Blot: HuC Antibody - Azide and BSA Free [HuC] - Western blot analysis of various lysates, using HuC Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.8s.](http://images.novusbio.com/fullsize/nbp3-02943_rabbit-huc-pab-52202516321480.jpg "Western Blot: HuC Antibody - Azide and BSA Free [HuC] - Western blot analysis of various lysates, using HuC Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.8s.")
![Immunohistochemistry-Paraffin: HuC Antibody [NBP3-02943] - Immunohistochemistry of paraffin-embedded mouse brain using HuC antibody (NBP3-02943) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HuC-Antibody-Immunohistochemistry-Paraffin-NBP3-02943-img0006.jpg)
![Immunohistochemistry-Paraffin: HuC Antibody [NBP3-02943] - Paraffin-embedded rat brain using HuC antibody at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/HuC-Antibody-Immunohistochemistry-Paraffin-NBP3-02943-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: HuC Antibody - Azide and BSA Free [HuC] - Immunofluorescence analysis of paraffin-embedded mouse brain using HuC Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-02943_rabbit-huc-pab-52202516321493.jpg)
![Western Blot: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Analysis of extracts of various cell lines, using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-8T4D3-Western-Blot-NBP3-15454-img0002.jpg "Western Blot: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Analysis of extracts of various cell lines, using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Immunofluorescence analysis of Hep G2 cells using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-8T4D3-Immunocytochemistry-Immunofluorescence-NBP3-15454-img0001.jpg)
![Western Blot: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Analysis of extracts of various cell lines, using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-8T4D3-Western-Blot-NBP3-15454-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog/Shh Antibody (8T4D3) [Sonic Hedgehog/Shh] - Confocal imaging of paraffin-embedded Mouse kidney using Sonic Hedgehog/Shh Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15454_rabbit-sonic-hedgehog-shh-mab-8t4d3-522025171899.jpg)
![Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog/Shh Antibody (8T4D3) [Sonic Hedgehog/Shh] - Confocal imaging of Hep G2 cells using Sonic Hedgehog/Shh Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15454_rabbit-sonic-hedgehog-shh-mab-8t4d3-52202517192951.jpg)