| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95093-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45345 | |||
| Azide and BSA Free | NBP2-34733-0.1mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 647 | DDX0141A647-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46642 | |||
| BSA Free | NBP2-46642-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59779 | |||
| BSA Free | NBP2-59779-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36770 | |||
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Analysis of extracts of various cell lines, using Sodium Potassium ATPase Beta 1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Western-Blot-NBP2-95093-img0004.jpg "Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Analysis of extracts of various cell lines, using Sodium Potassium ATPase Beta 1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Paraffin-embedded mouse stomach using Sodium Potassium ATPase Beta 1 .](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95093-img0003.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Paraffin-embedded human colon carcinoma using Sodium Potassium ATPase Beta 1 .](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95093-img0001.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Paraffin-embedded human gastric cancer using Sodium Potassium ATPase Beta 1 .](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95093-img0002.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] - Western blot analysis of various lysates using Sodium Potassium ATPase Beta 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp2-95093_rabbit-polyclonal-sodium-potassium-atpase-beta-1-antibody-52202516345269.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] - Immunofluorescence analysis of NIH/3T3 cells using Sodium Potassium ATPase Beta 1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95093_rabbit-polyclonal-sodium-potassium-atpase-beta-1-antibody-5220251638037.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] - Immunofluorescence analysis of C6 cells using Sodium Potassium ATPase Beta 1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95093_rabbit-polyclonal-sodium-potassium-atpase-beta-1-antibody-5220251628041.jpg)
![Western Blot: RPE65 Antibody (5X1F6) [NBP3-16840] - Analysis of extracts of Rat eye, using RPE65 Rabbit mAb (NBP3-16840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Western-Blot-NBP3-16840-img0005.jpg "Western Blot: RPE65 Antibody (5X1F6) [NBP3-16840] - Analysis of extracts of Rat eye, using RPE65 Rabbit mAb (NBP3-16840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of mouse eye using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-NBP3-16840-img0004.jpg)
![Immunohistochemistry-Paraffin: RPE65 Antibody (5X1F6) [NBP3-16840] - Rat retina using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-Paraffin-NBP3-16840-img0001.jpg)
![Immunohistochemistry-Paraffin: RPE65 Antibody (5X1F6) [NBP3-16840] - Mouse retina using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-Paraffin-NBP3-16840-img0002.jpg)
![Immunohistochemistry: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of rat eye using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-NBP3-16840-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of paraffin-embedded rat eye using RPE65 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16840_rabbit-rpe65-mab-5x1f6-52202511361295.jpg)
![Western Blot: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - All lanes: Western blot analysis of Cytokeratin 19 with anti-Cytokeratin 19 antibody [SA30-06] (NBP3-32248) at 1:5,000 dilution. Lane 1: Wild-type HepG2 whole cell lysate (20 ug). Lane 2/3: Cytokeratin 19 knockout HepG2 whole cell lysate (20 ug). NBP3-32248 was shown to specifically react with Cytokeratin 19 in wild-type HepG2 cells. No band was observed when Cytokeratin 19 knockout samples were tested. Wild-type and Cytokeratin 19 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Cytokeratin 19 Antibody (ET1601-6, 1/5,000) and anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-278202416285243.jpg "Western Blot: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - All lanes: Western blot analysis of Cytokeratin 19 with anti-Cytokeratin 19 antibody [SA30-06] (NBP3-32248) at 1:5,000 dilution. Lane 1: Wild-type HepG2 whole cell lysate (20 ug). Lane 2/3: Cytokeratin 19 knockout HepG2 whole cell lysate (20 ug). NBP3-32248 was shown to specifically react with Cytokeratin 19 in wild-type HepG2 cells. No band was observed when Cytokeratin 19 knockout samples were tested. Wild-type and Cytokeratin 19 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Cytokeratin 19 Antibody (ET1601-6, 1/5,000) and anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Cytokeratin 19 antibody (NBP3-32248) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32248) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-298202414115431.jpg)
![Immunohistochemistry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Cytokeratin 19 (NBP3-32248). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 19 (NBP3-32248, red) at 1/500 dilution at +4℃ overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-298202414333689.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - ICC staining of Cytokeratin 19 in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32248, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-29202410484831.jpg)
![Flow Cytometry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Flow cytometric analysis of Cytokeratin 19 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (NBP3-32248, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-39202488466.jpg)
![Western Blot: GPM6A Antibody [NBP2-85000] - Lanes: Lane1: 250ug rat hippocampal culture neurons. Lane2: 200ug rat hippocampal culture neurons. Lane3: 100ug rat hippocampal culture neurons. Lane4: 50ug rat hippocampal culture neurons. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:8000. Gene Name: GPM6A. Submitted by: Anonymous.](http://images.novusbio.com/fullsize/GPM6A-Antibody-Western-Blot-NBP2-85000-img0005.jpg "Western Blot: GPM6A Antibody [NBP2-85000] - Lanes: Lane1: 250ug rat hippocampal culture neurons. Lane2: 200ug rat hippocampal culture neurons. Lane3: 100ug rat hippocampal culture neurons. Lane4: 50ug rat hippocampal culture neurons. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:8000. Gene Name: GPM6A. Submitted by: Anonymous.")
![Immunohistochemistry: GPM6A Antibody [NBP2-85000] - Primary Antibody Dilution: 1:250. Secondary Antibody: Anti-rabbit-AlexaFluor 488. Secondary Antibody Dilution: 1:1000. Color/Signal Descriptions: GPM6A: Green DAPI: Blue. Gene Name: GPM6A. Submitted by: Anonymous](http://images.novusbio.com/fullsize/GPM6A-Antibody-Immunohistochemistry-NBP2-85000-img0007.jpg)
![Western Blot: GPM6A Antibody [NBP2-85000] - Host: Rabbit. Target Name: GPM6A. Antibody Dilution: 1.0ug/ml. Sample Type: Human brain](http://images.novusbio.com/fullsize/GPM6A-Antibody-Western-Blot-NBP2-85000-img0001.jpg)
![Western Blot: GPM6A Antibody [NBP2-85000] - Host: Rabbit. Target: GPM6A. Positive control (+): Human Liver (LI). Negative control (-): Human Stomach Tumor (T-ST). Antibody concentration: 3ug/ml](http://images.novusbio.com/fullsize/GPM6A-Antibody-Western-Blot-NBP2-85000-img0003.jpg)
![Western Blot: GPM6A Antibody [NBP2-85000] - Host: Rabbit. Target Name: GPM6A. Sample Tissue: Human Brain. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/GPM6A-Antibody-Western-Blot-NBP2-85000-img0002.jpg)
![Western Blot: GPM6A Antibody [NBP2-85000] - Lanes: Lane1: 400ug rat hippocampal. Lane2: 300ug rat hippocampal. Lane3: 200ug rat hippocampal. Lane4: 100ug rat hippocampal. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:8000. Gene Name: GPM6A. Submitted by: Anonymous.](http://images.novusbio.com/fullsize/GPM6A-Antibody-Western-Blot-NBP2-85000-img0004.jpg)
![Immunohistochemistry: GPM6A Antibody [NBP2-85000] - Primary Antibody Dilution: 1:250. Secondary Antibody: Anti-rabbit-AlexaFluor 488. Secondary Antibody Dilution: 1:1000. Color/Signal Descriptions: GPM6A: Green DAPI: Blue. Gene Name: GPM6A. Submitted by: Anonymous](http://images.novusbio.com/fullsize/GPM6A-Antibody-Immunohistochemistry-NBP2-85000-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SPM123) [NBP2-32933] - Formalin-fixed paraffin-embedded human tonsil stained with CD34 Monoclonal Antibody (SPM123)](http://images.novusbio.com/fullsize/CD34-Antibody-SPM123-Immunohistochemistry-Paraffin-NBP2-32933-img0003.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (SPM123) [NBP2-32933] - Formalin-fixed paraffin-embedded human tonsil stained with CD34 Monoclonal Antibody (SPM123)")
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Immunocytochemistry-Immunofluorescence-DDX0141P-100-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone")
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - FACS staining of hCD40L-transfected Lcellswith LL2 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Flow-Cytometry-DDX0141P-100-img0002.jpg)
![Blocking/Neutralizing: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - LL2 inhibitsthe stimulatory effect of the CD40L-transfected L cells on synovial fibroblasts Rissoan et al, Clin Exp Immunol. 1996, 106(3):481-90.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Blocking-Neutralizing-DDX0141P-100-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Kidney tissue](http://images.novusbio.com/fullsize/nbp3-43802_mouse-dopa-decarboxylase-ddc-mab-cl2962-azide-bsa-free-28220251273749.jpg)
![Untreated (-) and treated (+) unboiled HL-60 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-1411202518415223.jpg "<b>Orthogonal Strategies Validation. </b>Untreated (-) and treated (+) unboiled HL-60 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Boiled and unboiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-1411202518335915.jpg)
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-141120251842516.jpg)
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:5000 and competitor's antibody (competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-141120251839419.jpg)
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-1411202518392014.jpg)
![P2Y2 antibody [HL2704] detects P2Y2 protein at cell membrane by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-immunocytochemistry-immunofluorescence-1411202518333113.jpg)
![<b>Orthogonal Strategies Validation. </b>Boiled and unboiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-141120251842412.jpg)
![Western Blot: GAPDH Antibody (BLR103H) [NBP3-14695] - Whole cell lysate (15 ug) from EL4, mIMCD-3, RenCa, NIH 3T3, TCMK-1, C2C12, A20, and Ba/F3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GAPDH recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-BLR103H-Western-Blot-NBP3-14695-img0002.jpg "Western Blot: GAPDH Antibody (BLR103H) [NBP3-14695] - Whole cell lysate (15 ug) from EL4, mIMCD-3, RenCa, NIH 3T3, TCMK-1, C2C12, A20, and Ba/F3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GAPDH recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry: GAPDH Antibody (BLR103H) [NBP3-14695] - Rabbit anti-GAPDH recombinant monoclonal antibody. Secondary HRP-conjugated goat anti-rabbit IgG. Substrate:DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-BLR103H-Immunohistochemistry-NBP3-14695-img0003.jpg)
![Western Blot: GAPDH Antibody (BLR103H) [NBP3-14695] - Whole cell lysate (50 ug) from HeLa, Jurkat, RKO, U2OS, GaMG, LNCaP, MCF-7, K-562, HEK293T, and Hep-G2 cells prepared using NETN lysis buffer. Rabbit anti-GAPDH recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/GAPDH-Antibody-BLR103H-Western-Blot-NBP3-14695-img0001.jpg)
![ICC/IF-GAPDH Antibody (BLR103H)-NBP3-14695-: Rabbit anti-GAPDH recombinant monoclonalantibody [BLR103H]. Secondary: HRP conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14695_rabbit-gapdh-mab-immunocytochemistry-immunofluorescence-199202311112..jpg)
![Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-2120249415947.jpg "Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - SPG7 antibody [HL2236] detects SPG7 protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: SPG7 stained by SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-212024941595.jpg)
![Immunohistochemistry-Paraffin: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - SPG7 antibody [HL2236] detects SPG7 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. SPG7 stained by SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:20. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-212024941493.jpg)
![Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-2120249422671.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-212024943554.jpg)