| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48512-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49246-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38508-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79835 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85520-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90322-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70335 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12882-50ug | |||
![Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-48512] - Staining in human cerebral cortex and pancreas tissues using anti-MAP1LC3A antibody. Corresponding MAP1LC3A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-Paraffin-NBP2-48512-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-48512] - Staining in human cerebral cortex and pancreas tissues using anti-MAP1LC3A antibody. Corresponding MAP1LC3A RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: LC3A Antibody [NBP2-48512] - Analysis in control (vector only transfected HEK293T lysate) and MAP1LC3A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/LC3A-Antibody-Western-Blot-NBP2-48512-img0006.jpg)
![Western Blot: LC3A Antibody [NBP2-48512] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/LC3A-Antibody-Western-Blot-NBP2-48512-img0003.jpg)
![Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-48512] - Staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-Paraffin-NBP2-48512-img0001.jpg)
![Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-48512] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-Paraffin-NBP2-48512-img0004.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP2-49246] - Analysis in human heart muscle and liver tissues using NBP2-49246 antibody. Corresponding CRYAB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunohistochemistry-Paraffin-NBP2-49246-img0002.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP2-49246] - Analysis in human heart muscle and liver tissues using NBP2-49246 antibody. Corresponding CRYAB RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP2-49246] - Staining of human liver shows very weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunohistochemistry-Paraffin-NBP2-49246-img0006.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP2-49246] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunohistochemistry-Paraffin-NBP2-49246-img0003.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP2-49246] - Staining of human skeletal muscle shows moderate ctyoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunohistochemistry-Paraffin-NBP2-49246-img0004.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP2-49246] - Staining of human pancreas shows very weak cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunohistochemistry-Paraffin-NBP2-49246-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PrPC Antibody [NBP2-38508] - Staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38508-img0003.jpg "Immunocytochemistry/Immunofluorescence: PrPC Antibody [NBP2-38508] - Staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: PrPC Antibody [NBP2-38508] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/PrPC-Antibody-Immunohistochemistry-Paraffin-NBP2-38508-img0007.jpg)
![Immunohistochemistry-Paraffin: PrPC Antibody [NBP2-38508] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-Immunohistochemistry-NBP2-38508-img0002.jpg)
![Immunohistochemistry-Paraffin: PrPC Antibody [NBP2-38508] - Staining of human cerebral cortex shows strong granuclar cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/PrPC-Antibody-Immunohistochemistry-Paraffin-NBP2-38508-img0005.jpg)
![Immunohistochemistry-Paraffin: PrPC Antibody [NBP2-38508] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/PrPC-Antibody-Immunohistochemistry-Paraffin-NBP2-38508-img0006.jpg)
![Western Blot: Calnexin Antibody (CANX/1543) [NBP2-79701] - Western Blot of Analysis of human Kidney lysate using Calnexin Antibody (CANX/1543).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1543-Western-Blot-NBP2-79701-img0004.jpg "Western Blot: Calnexin Antibody (CANX/1543) [NBP2-79701] - Western Blot of Analysis of human Kidney lysate using Calnexin Antibody (CANX/1543).")
![Immunohistochemistry-Paraffin: Calnexin Antibody (CANX/1543) [NBP2-79701] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Calnexin Antibody (CANX/1543).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1543-Immunohistochemistry-Paraffin-NBP2-79701-img0001.jpg)
![Western Blot: Calnexin Antibody (CANX/1543) [NBP2-79701] - Western Blot of Analysis of PANC1 and MCF-7 cell lysate using Calnexin Antibody (CANX/1543).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1543-Western-Blot-NBP2-79701-img0003.jpg)
![Protein Array: Calnexin Antibody (CANX/1543) [NBP2-79701] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Calnexin Antibody (CANX/1543). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1543-Protein-Array-NBP2-79701-img0002.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85520-img0016.jpg "Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.")
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85520-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human cerebral cortex, placenta, testis and tonsil using Anti-Calnexin antibody NBP1-85520 (A) shows similar protein distribution across tissues to independent antibody NBP1-85519 (B).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85520-img0013.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85520-img0014.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85520-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HSP27 Antibody [NBP1-89666] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunohistochemistry-Paraffin-NBP1-89666-img0009.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NBP1-89666] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunohistochemistry-Paraffin-NBP1-89666-img0010.jpg)
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2498] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-western-blot-152025102720.jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2498] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Untreated (–) and treated (+) Mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2498] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-western-blot-152025103434..jpg)
![Immunohistochemistry: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Tau (p Thr181) antibody [HL2498] detects Tau (phospho Thr181) protein in the cytoplasm by immunohistochemical analysis in the presence of non phospho control peptide (left) but not in the presence of the antigenic phospho peptide (right).Sample: Paraffin-embedded mouse brain.Tau (p Thr181) stained by Tau (p Thr181) antibody [HL2498] diluted at 1:100.](http://images.novusbio.com/fullsize/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-immunohistochemistry-152025103033..jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Sandwich ELISA detection of Tau peptide and Tau (phospho Thr181) peptide using antibodies as below.Capture: Tau (phospho Thr181) antibody [HL2498] (NBP3-45158) (5 ug/mL)Detection: HRP-conjugated Tau (phospho Thr181) antibody [HL2497] (NBP3-45157) (1 ug/mL)](http://images.novusbio.com/fullsize/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-elisa-152025104331..jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2498) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.Capture: Tau (p Thr181) antibody [HL2497] (NBP3-45157) (5 ug/mL)Detection: HRP-conjugated Tau (p Thr181) antibody [HL2498] (NBP3-45158) (1 ug/mL)](http://images.novusbio.com/fullsize/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-elisa-152025105927..jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 5-HT2B Antibody [NBP1-90322] - Staining of human endometrium shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NBP1-90322-img0007.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NBP1-90322] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NBP1-90322-img0010.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NBP1-90322] - Staining of human kidney shows moderate membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NBP1-90322-img0008.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NBP1-90322] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NBP1-90322-img0009.jpg)
![Western Blot: Calpain 2 Antibody (1F4) [NBP2-01814] Analysis of extracts (35ug) from 9 different cell lines by using anti-Calpain 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Calpain-2-Antibody-1F4-Western-Blot-NBP2-01814-img0005.jpg "Western Blot: Calpain 2 Antibody (1F4) [NBP2-01814] Analysis of extracts (35ug) from 9 different cell lines by using anti-Calpain 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Flow Cytometry: Calpain 2 Antibody (1F4) [NBP2-01814] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Calpain 2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-1F4-Flow-Cytometry-NBP2-01814-img0003.jpg)
![Western Blot: Calpain 2 Antibody (1F4) [NBP2-01814] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Calpain 2.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-1F4-Western-Blot-NBP2-01814-img0004.jpg)
![Flow Cytometry: Calpain 2 Antibody (1F4) [NBP2-01814] - Analysis of Hela cells, using anti-Calpain 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Calpain-2-Antibody-1F4-Flow-Cytometry-NBP2-01814-img0001.jpg)
![Flow Cytometry: Calpain 2 Antibody (1F4) [NBP2-01814] - Analysis of Jurkat cells, using anti-Calpain 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Calpain-2-Antibody-1F4-Flow-Cytometry-NBP2-01814-img0002.jpg)
![Western Blot: HDAC6 Antibody [NBP1-69127] - WB Suggested Anti-Hdac6 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Mouse Liver](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NBP1-69127-img0001.jpg "Western Blot: HDAC6 Antibody [NBP1-69127] - WB Suggested Anti-Hdac6 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Mouse Liver")
![Immunohistochemistry: HDAC6 Antibody [NBP1-69127] - Human Pineal Tissue.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69127-img0004.jpg)
![Immunohistochemistry: HDAC6 Antibody [NBP1-69127] - Human Adult heart Observed Staining: Cytoplasmic Primary Antibody Concentration: 1 : 100 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 2.0 sec.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunohistochemistry-NBP1-69127-img0005.jpg)
![Immunohistochemistry-Paraffin: HDAC6 Antibody [NBP1-69127] - Human testis tissue at an antibody concentration of 4-8ug/ml.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunohistochemistry-Paraffin-NBP1-69127-img0003.jpg)
![Western Blot: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - analysis of Human Cell lysates showing detection of Ubiquitin protein using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone 6C11-B3 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-6C11-B3-Western-Blot-NBP2-12882-img0003.jpg "Western Blot: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - analysis of Human Cell lysates showing detection of Ubiquitin protein using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone 6C11-B3 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin B Monoclonal Antibody, Clone 6C11-B3 (NBP2-12882). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin B Monoclonal Antibody (NBP2-12882) at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin B Antibody. (C) Composite.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-6C11-B3-Immunocytochemistry-Immunofluorescence-NBP2-12882-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-6C11-B3-Immunocytochemistry-Immunofluorescence-NBP2-12882-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin B Monoclonal Antibody, Clone 6C11-B3 (NBP2-12882). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin B Monoclonal Antibody (NBP2-12882) at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin B Antibody. (C) Composite.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-6C11-B3-Immunocytochemistry-Immunofluorescence-NBP2-12882-img0007.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32538) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-29820241420974.jpg "Immunohistochemistry: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32538) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunocytochemistry analysis of HeLa cells labeling LIMPII/SR-B2 with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/200 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-292024119239.jpg)
![Western Blot: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Western blot analysis of LIMPII/SR-B2 on different lysates with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: SH-SY5Y cell lysate (15 µg/Lane) Lane 4: Neuro-2a cell lysate (15 µg/Lane) Lane 5: MEF cell lysate (15 µg/Lane) Lane 6: PC-12 cell lysate (15 µg/Lane) Lane 7: COS-1 cell lysate (15 µg/Lane) Lane 8: Mouse kidney tissue lysate (30 µg/Lane) Lane 9: Rat kidney tissue lysate (30 µg/Lane) Lane 10: Mouse liver tissue lysate (30 µg/Lane) Lane 11: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 54 kDa Observed band size: 80 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-392024845352.jpg)