| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12909-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47146SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95200-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92597-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12918-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82063-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-20225-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71782 | |||
![Immunohistochemistry-Paraffin: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - TREM2 antibody [HL1738] detects TREM2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TREM2 stained by TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-212024940820.jpg "Immunohistochemistry-Paraffin: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - TREM2 antibody [HL1738] detects TREM2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TREM2 stained by TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - TREM2 antibody [HL1738] detects TREM2 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TREM2 stained by TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:250. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-2120249425467.jpg)
![Western Blot: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-2120249422617.jpg)
![Sandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below. Capture: TREM2 antibody [HL1739] (1 ug/mL)Please notice that NBP3-25728 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637387. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-elisa-1411202518443414.jpg)
![Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human TREM2 protein (1215.95-19.07 pM). Coated protein was probed with TREM2 antibody [HL1738] (NBP3-25728) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-elisa-1411202518414124.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-western-blot-14112025184488.jpg)
![Sandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below. Capture: TREM2 antibody [HL1738] (NBP3-25728) (5 ug/mL) Detection: HRP-conjugated TREM2 antibody [HL1739] (1 ug/mL)Please notice that GTX637387 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25728. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-elisa-141120251843335.jpg)
![Western Blot: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909] - Western Blot analysis of Human Cell lysates showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (NBP2-12909). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12909) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-S15-4-Western-Blot-NBP2-12909-img0008.jpg "Western Blot: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909] - Western Blot analysis of Human Cell lysates showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (NBP2-12909). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12909) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (NBP2-12909). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12909) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpV3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-S15-4-Immunocytochemistry-Immunofluorescence-NBP2-12909-img0011.jpg)
![Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909] - Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (NBP2-12909). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12909) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-S15-4-Immunohistochemistry-NBP2-12909-img0010.jpg)
![Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909] - Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (NBP2-12909). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12909) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-S15-4-Immunohistochemistry-NBP2-12909-img0009.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining in human appendix and skeletal muscle tissues using anti-NFKB2 antibody. Corresponding NFKB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining in human appendix and skeletal muscle tissues using anti-NFKB2 antibody. Corresponding NFKB2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-NFKB2 antibody NBP1-87759 (A) shows similar protein distribution across tissues to independent antibody NBP1-87760 (B).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: NFkB p100/p52 Antibody [NBP1-87759] - Western blot analysis in human cell lines A-431 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NBP1-87759-img0009.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human kidney.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0014.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human appendix shows high expression.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0006.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0007.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human lymph node.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0012.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human testis.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0013.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human cerebral cortex using Anti-NFKB2 antibody.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0015.jpg)
![Western Blot: C9orf72 Antibody [NBP2-47146] - Western Blot Image of anti-C9orf72. Whole cell protein from SK-N-SH (lane 1) and SH-SY-5Y (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-hnRNP in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/C9orf72-Antibody-Western-Blot-NBP2-47146-img0001.jpg "Western Blot: C9orf72 Antibody [NBP2-47146] - Western Blot Image of anti-C9orf72. Whole cell protein from SK-N-SH (lane 1) and SH-SY-5Y (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-hnRNP in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: C9orf72 Antibody [NBP2-47146] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-C9orf72 (NBP2-47146) at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/C9orf72-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47146-img0002.jpg)
![Immunohistochemistry-Paraffin: C9orf72 Antibody [NBP2-47146] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using C9orf72 antibody (NBP2-47146) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated strong/specific cytoplasmic signal in the neurons and other cell types in the tested section.](http://images.novusbio.com/fullsize/C9orf72-Antibody-Immunohistochemistry-Paraffin-NBP2-47146-img0003.jpg)
![Western Blot: C9orf72 Antibody (GT1553-RB) [NBP3-13658] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553-RB] (NBP3-13658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/C9orf72-Antibody-GT1553-RB-Western-Blot-NBP3-13658-img0001.jpg "Western Blot: C9orf72 Antibody (GT1553-RB) [NBP3-13658] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553-RB] (NBP3-13658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![C9orf72 Antibody (1553-RB) [NBP3-13658] - DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: C9orf72 stained by C9orf72 antibody [1553-RB] diluted at 1:250.Red: Tau, a cytoskeleton marker, stained by Tau antibody [287] diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp3-13658_rabbit-c9orf72-mab-gt1553-rb-immunocytochemistry-immunofluorescence-132202483135..jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: C9orf72 Antibody (GT1553-RB) [NBP3-13658] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553-RB] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13658_rabbit-c9orf72-mab-gt1553-rb-1610202419414565.jpg)
![Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553-RB] (NBP3-13658) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13658_rabbit-c9orf72-mab-gt1553-rb-western-blot-2611202518243914.jpg)
![C9orf72 antibody [GT1553-RB] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.C9orf72 stained by C9orf72 antibody [GT1553-RB] (NBP3-13658) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13658_rabbit-c9orf72-mab-gt1553-rb-immunohistochemistry-paraffin-2611202518321916.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553-RB] (NBP3-13658) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13658_rabbit-c9orf72-mab-gt1553-rb-western-blot-2611202518381511.jpg)
![Western Blot: NFkB p100/p52 Antibody [NBP2-95200] - Analysis of extracts of various cell lines, using NFkB p100/p52 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NBP2-95200-img0005.jpg "Western Blot: NFkB p100/p52 Antibody [NBP2-95200] - Analysis of extracts of various cell lines, using NFkB p100/p52 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: NFkB p100/p52 Antibody [NBP2-95200] - Analysis of U2OS cells using NFkB p100/p52 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-95200-img0001.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP2-95200] - Rat brain using NF-kB2 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP2-95200-img0004.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP2-95200] - Human breast using NF-KB2 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP2-95200-img0002.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP2-95200] - Mouse testis using NF-kB2 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP2-95200-img0003.jpg)
![Western Blot: DOK4 Antibody [NBP2-92597] - Western blot analysis of extracts of various cell lines, using DOK4 antibody (NBP2-92597) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/DOK4 Antibody-Western Blot-NBP2-92597-img0002.jpg "Western Blot: DOK4 Antibody [NBP2-92597] - Western blot analysis of extracts of various cell lines, using DOK4 antibody (NBP2-92597) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: DOK4 Antibody [NBP2-92597] - Immunohistochemistry of paraffin-embedded Human colon carcinoma using DOK4 Rabbit pAb (NBP2-92597) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DOK4-Antibody-Immunohistochemistry-Paraffin-NBP2-92597-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: DOK4 Antibody - Azide and BSA Free [NBP2-92597] - Immunofluorescence analysis of PC-12 cells using DOK4 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92597_rabbit-polyclonal-dok4-antibody-42202514264447.jpg)
![Immunocytochemistry/ Immunofluorescence: DOK4 Antibody - Azide and BSA Free [NBP2-92597] - Immunofluorescence analysis of NIH/3T3 cells using DOK4 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92597_rabbit-polyclonal-dok4-antibody-4220251430491.jpg)
![Immunocytochemistry/ Immunofluorescence: DOK4 Antibody - Azide and BSA Free [NBP2-92597] - Immunofluorescence analysis of SH-SY5Y cells using DOK4 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92597_rabbit-polyclonal-dok4-antibody-4220251430463.jpg)
![Western Blot: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Western Blot analysis of Rat brain membrane lysate showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-N15-39-Western-Blot-NBP2-12918-img0006.jpg "Western Blot: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Western Blot analysis of Rat brain membrane lysate showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpV3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-N15-39-Immunocytochemistry-Immunofluorescence-NBP2-12918-img0008.jpg)
![Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining in upper layers. Dull lower layer cell staining. Some stain seen in hypodermis.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-N15-39-Immunohistochemistry-NBP2-12918-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dotty staining in all cells. Some intermediate filamentlike staining in some cells.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-N15-39-Immunocytochemistry-Immunofluorescence-NBP2-12918-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NFkB p100/p52 Antibody [NB100-82063] - Staining of methanol-fixed Hela cells using NB100-82063](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82063-img0004.jpg "Immunocytochemistry/Immunofluorescence: NFkB p100/p52 Antibody [NB100-82063] - Staining of methanol-fixed Hela cells using NB100-82063")
![Western Blot: NFkB p100/p52 Antibody [NB100-82063] - Extracts from HT29 cells using NB100-82063 and the same antibody preincubated with blocking peptide](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NB100-82063-img0008.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NB100-82063] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NB100-82063(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-82063_rabbit-polyclonal-nfkb-p100-p52-antibody-103202523464553.jpg)
![Western Blot: Spectrin beta 2 Antibody [NB100-40828] - Detection of mouse SPTBN1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN1 antibody NB100-40828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Western-Blot-NB100-40828-img0005.jpg "Western Blot: Spectrin beta 2 Antibody [NB100-40828] - Detection of mouse SPTBN1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN1 antibody NB100-40828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: Spectrin beta 2 Antibody [NB100-40828] - Detection of human SPTBN1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SPTBN1 (NB100-40828). Detection: DAB](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Immunohistochemistry-NB100-40828-img0006.jpg)
![Western Blot: Spectrin beta 2 Antibody [NB100-40828] - Detection of human SPTBN1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN1 antibody NB100-40828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Western-Blot-NB100-40828-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Spectrin beta 2 Antibody [NB100-40828] - Detection of human Spectrin beta 2 (SPTBN1) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Spectrin beta 2 antibody NB100-40828 used for IP at 3 ug per reaction. SPTBN1 was also immunoprecipitated by rabbit anti-Spectrin beta 2 antibody NB100-40829. For blotting immunoprecipitated SPTBN1, NB100-40828 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Immunoprecipitation-NB100-40828-img0007.jpg)
![Western Blot: TRPV4 Antibody [NBP1-20225] - WB on mouse brain lysate (reduced, denatured) using Rabbit antibody to TRPV4 at 1 : 1000 dilution.](http://images.novusbio.com/fullsize/TRPV4-Antibody-Western-Blot-NBP1-20225-img0003.jpg "Western Blot: TRPV4 Antibody [NBP1-20225] - WB on mouse brain lysate (reduced, denatured) using Rabbit antibody to TRPV4 at 1 : 1000 dilution.")
![Immunohistochemistry-Paraffin: TRPV4 Antibody [NBP1-20225] - IHC on paraffin sections of rat kidney: Animal was perfused with perfuser at 100 mmHg with 300 ml PBS and then 4%PFA. Tissues were extracted and further fixed for 2 hours at room temperature before being embedded in paraffin. HIER: 1 mM EDTA, pH 8 for 20 min. Detection was done using HRP polymer following manufactures instruction Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)](http://images.novusbio.com/fullsize/TRPV4-Antibody-Immunohistochemistry-Paraffin-NBP1-20225-img0002.jpg)
![Immunohistochemistry-Paraffin: TRPV4 Antibody [NBP1-20225] - IHC on paraffin sections of rat kidney: Animal was perfused with perfuser at 100 mmHg with 300 ml PBS and then 4%PFA. Tissues were extracted and further fixed for 2 hours at room temperature before being embedded in paraffin. HIER: 1 mM EDTA, pH 8 for 20 min. Detection was done using HRP polymer following manufactures instruction Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)](http://images.novusbio.com/fullsize/TRPV4-Antibody-Immunohistochemistry-Paraffin-NBP1-20225-img0001.jpg)
![Western Blot: APPL Antibody (OTI4B11) [NBP2-46536] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APPL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APPL1.](http://images.novusbio.com/fullsize/APPL-Antibody-OTI4B11-Western-Blot-NBP2-46536-img0001.jpg "Western Blot: APPL Antibody (OTI4B11) [NBP2-46536] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APPL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APPL1.")
![Immunohistochemistry-Paraffin: APPL Antibody (OTI4B11) [NBP2-46536] - Staining of paraffin-embedded Human lymphoma tissue using anti-APPL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/APPL-Antibody-OTI4B11-Immunohistochemistry-Paraffin-NBP2-46536-img0004.jpg)
![Immunohistochemistry-Paraffin: APPL Antibody (OTI4B11) [NBP2-46536] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-APPL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/APPL-Antibody-OTI4B11-Immunohistochemistry-Paraffin-NBP2-46536-img0002.jpg)
![Immunohistochemistry-Paraffin: APPL Antibody (OTI4B11) [NBP2-46536] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-APPL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/APPL-Antibody-OTI4B11-Immunohistochemistry-Paraffin-NBP2-46536-img0003.jpg)