| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78966SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81027-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88972-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-43666-25ul | |||
| Azide and BSA Free | NBP3-44172 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 647 | DDX0141A647-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50060SS | |||
![Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Western-Blot-NBP2-66756-img0007.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0006.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of paraffin-embedded human spleen tissue using anti- CD40L antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunohistochemistry-Paraffin-NBP2-66756-img0004.jpg)
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of THP-1 cells with CD40L antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Flow-Cytometry-NBP2-66756-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0003.jpg)
![Western Blot: TET1 Antibody [NBP2-19290] - WB analysis of lysates from 293T cell which were mock transfected (lane1) or with tagged-mTet1 (lane 2) for 24hrs, using either TET1 antibody (diluted at 1:2000) (A) or anti-tag (B) antibody.](http://images.novusbio.com/fullsize/TET1-Antibody-Western-Blot-NBP2-19290-img0003.jpg "Western Blot: TET1 Antibody [NBP2-19290] - WB analysis of lysates from 293T cell which were mock transfected (lane1) or with tagged-mTet1 (lane 2) for 24hrs, using either TET1 antibody (diluted at 1:2000) (A) or anti-tag (B) antibody.")
![Western Blot: TET1 Antibody [NBP2-19290] - WB validation of TET1 antibody on 30 ug whole cell extracts of non-transfected (-) and mouse TET1-transfected (+, including DDDDK-tag) 293T cells. The proteins were separated on 5% SDS-PAGE, and the membrane was blotted with TET1 antibody at a dilution of 1:20000.](http://images.novusbio.com/fullsize/TET1-Antibody-Western-Blot-NBP2-19290-img0001.jpg)
![TET1 antibody [N1], N-term detects TET1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.TET1 stained by TET1 antibody [N1], N-term (NBP2-19290) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19290_rabbit-polyclonal-tet1-antibody-immunohistochemistry-paraffin-261120251838404.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - IF staining of hCD40L-transfected L cells with LL48 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL48-Immunocytochemistry-Immunofluorescence-DDX0140P-100-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - IF staining of hCD40L-transfected L cells with LL48 clone")
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - FACS staining of hCD40L-transfected L cells with LL48 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL48-Flow-Cytometry-DDX0140P-100-img0002.jpg)
![Neutralization: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - Inhibition of IL12 production by CD40L-stimulated human monocyte-derived DCs by LL48 clone (Stax AM et al, 2008, J ImmunolMethods)](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL48-Neutralization-DDX0140P-100-img0003.jpg)
![Immunohistochemistry: TET1 Antibody [NBP1-78966] - Analysis of TET1 in mouse spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-NBP1-78966-img0001.jpg "Immunohistochemistry: TET1 Antibody [NBP1-78966] - Analysis of TET1 in mouse spleen using DAB with hematoxylin counterstain.")
![Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Analysis in control (vector only transfected HEK293T lysate) and cD40LG over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Western-Blot-NBP1-91770-img0007.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Analysis in control (vector only transfected HEK293T lysate) and cD40LG over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells.")
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human rectum shows strong cytoplasmic positivity in leukocytes.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-Paraffin-NBP1-91770-img0011.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-Paraffin-NBP1-91770-img0008.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human lymph node shows strong cytoplasmic positivity in leukocytes.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-Paraffin-NBP1-91770-img0009.jpg)
![Immunohistochemistry: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-NBP1-91770-img0010.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human cerebellum, lymph node, prostate and testis using Anti-MeCP2 antibody NBP1-81027 (A) shows similar protein distribution across tissues to independent antibody NBP1-81028 (B).](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81027-img0014.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human cerebellum, lymph node, prostate and testis using Anti-MeCP2 antibody NBP1-81027 (A) shows similar protein distribution across tissues to independent antibody NBP1-81028 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NBP1-81027] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81027-img0005.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81027-img0017.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human cerebellum shows strong nuclear positivity in cells in granular layer.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81027-img0012.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81027-img0015.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human prostate shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81027-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: TET1 Antibody [NBP1-88972] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear membrane.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88972-img0005.jpg "Immunocytochemistry/Immunofluorescence: TET1 Antibody [NBP1-88972] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear membrane.")
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP1-88972] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-Paraffin-NBP1-88972-img0009.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP1-88972] - Staining of human cerebellum shows moderate nuclear positivity in purkinje cells.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-Paraffin-NBP1-88972-img0006.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP1-88972] - Staining of human cervix, uterine shows moderate nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-Paraffin-NBP1-88972-img0007.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP1-88972] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-Paraffin-NBP1-88972-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CGRP Antibody [NBP3-13330] - Calcitonin antibody detects Calcitonin protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcitonin stained by Calcitonin antibody (NBP3-13330) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/CGRP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13330-img0005.jpg "Immunocytochemistry/Immunofluorescence: CGRP Antibody [NBP3-13330] - Calcitonin antibody detects Calcitonin protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcitonin stained by Calcitonin antibody (NBP3-13330) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Frozen: CGRP Antibody [NBP3-13330] - Calcitonin antibody detects Calcitonin protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: Calcitonin stained by Calcitonin antibody (NBP3-13330) diluted at 1:250. Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/CGRP-Antibody-Immunohistochemistry-Frozen-NBP3-13330-img0004.jpg)
![Immunohistochemistry-Paraffin: CGRP Antibody [NBP3-13330] - Mouse spinal cord . Calcitonin stained by Calcitonin antibody (NBP3-13330) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CGRP-Antibody-Immunohistochemistry-Paraffin-NBP3-13330-img0001.jpg)
![Immunohistochemistry-Paraffin: CGRP Antibody [NBP3-13330] - Mouse brain. Calcitonin stained by Calcitonin antibody (NBP3-13330) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CGRP-Antibody-Immunohistochemistry-Paraffin-NBP3-13330-img0002.jpg)
![Immunohistochemistry-Paraffin: CGRP Antibody [NBP3-13330] - Rat brain. Calcitonin stained by Calcitonin antibody (NBP3-13330) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CGRP-Antibody-Immunohistochemistry-Paraffin-NBP3-13330-img0003.jpg)
![CGRP antibody detects CGRP protein at cytoplasmic vesicles by immunofluorescent analysis.Sample: mouse dorsal root ganglia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CGRP stained by CGRP antibody (NBP3-13330) diluted at 1:500.Red: NF-H, an axon marker, stained by NF-H antibody [GT114] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13330_rabbit-cgrp-pab-immunocytochemistry-immunofluorescence-261120251828108.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Immunocytochemistry-Immunofluorescence-DDX0141P-100-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone")
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - FACS staining of hCD40L-transfected Lcellswith LL2 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Flow-Cytometry-DDX0141P-100-img0002.jpg)
![Blocking/Neutralizing: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - LL2 inhibitsthe stimulatory effect of the CD40L-transfected L cells on synovial fibroblasts Rissoan et al, Clin Exp Immunol. 1996, 106(3):481-90.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Blocking-Neutralizing-DDX0141P-100-img0003.jpg)
![Western Blot: MeCP2 Antibody [NBP2-50060] - Analysis of tissue and cell lysates using rabbit pAb to MeCP2, NBP2-50060, dilution 1:20,000 in green: [1] protein standard (red), [2] Rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain, [6] HeLa cells, [7] C6 cells. Major band at about 70-75 kDa corresponds to MeCP2 protein, predominantly detected in the nuclear fraction of the lysates. The apparent molecular weights of MeCP2 based on SDS-PAGE mobility are variable between species and higher than the actual MeCP2 molecular weight which is ~54kDa](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NBP2-50060-img0005.jpg "Western Blot: MeCP2 Antibody [NBP2-50060] - Analysis of tissue and cell lysates using rabbit pAb to MeCP2, NBP2-50060, dilution 1:20,000 in green: [1] protein standard (red), [2] Rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain, [6] HeLa cells, [7] C6 cells. Major band at about 70-75 kDa corresponds to MeCP2 protein, predominantly detected in the nuclear fraction of the lysates. The apparent molecular weights of MeCP2 based on SDS-PAGE mobility are variable between species and higher than the actual MeCP2 molecular weight which is ~54kDa")
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NBP2-50060] - Mouse brain section, which was cut at 45um on a vibratome after perfusion with 4% paraformaldehyde and overnight fixation, was stained with rabbit polyclonal antibody NBP2-50060. MeCP2 was mainly associated with methylated DNA in nucleus of neuron cells (green). Blue shows DAPI staining of nuclear DNA.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50060-img0003.jpg)
![Immunohistochemistry Free-Floating: MeCP2 Antibody [NBP2-50060] - Analysis of rat cerebellum section costained with rabbit pAb to MeCP2, NBP2-50060, dilution 1:5,000, in red, and chicken pAb to calbindin, dilution 1:2,000, in green. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. The MeCP2 antibody selectively stains nuclei of neuronal cells. Calbindin, often used as Purkinje cell marker, is prominently expressed in dendrites and perikarya of these cells in the cerebellar molecular layer.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50060-img0004.jpg)
![Western Blot: MeCP2 Antibody [NBP2-50060] - Analysis of MeCP2 expression in nuclear extracts from mouse brain with NBP2-50060. This polyclonal antibody recognizes a strong and clear band at 75 kDa corresponding to total MeCP2 of mouse brain in SDS-PAGE, while molecular weight of MeCP2 protein is 54 kDa.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NBP2-50060-img0002.jpg)
![Western Blot: Kir6.2 Antibody [NBP2-76944] - Western blot analysis of Kir6.2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Human liver tissue lysateLane 2: Mouse liver tissue lysate](http://images.novusbio.com/fullsize/Kir6.2-Antibody-Western-Blot-NBP2-76944-img0001.jpg "Western Blot: Kir6.2 Antibody [NBP2-76944] - Western blot analysis of Kir6.2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Human liver tissue lysateLane 2: Mouse liver tissue lysate")
![Immunocytochemistry/Immunofluorescence: Kir6.2 Antibody [NBP2-76944] - ICC staining of Kir6.2 in LoVo cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluor488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/Kir6.2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76944-img0002.jpg)
![Immunohistochemistry: Kir6.2 Antibody [NBP2-76944] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Kir6.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Kir6.2-Antibody-Immunohistochemistry-NBP2-76944-img0005.jpg)
![Immunohistochemistry: Kir6.2 Antibody [NBP2-76944] - Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Kir6.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Kir6.2-Antibody-Immunohistochemistry-NBP2-76944-img0003.jpg)
![Immunohistochemistry: Kir6.2 Antibody [NBP2-76944] - Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Kir6.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Kir6.2-Antibody-Immunohistochemistry-NBP2-76944-img0004.jpg)