| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76905-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77021-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04863-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22410-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59374-25ug | |||
![Western Blot: RIMS2 Antibody [NBP1-76905] - Analysis in human brain tissue lysate with antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/RIMS2-Antibody-Western-Blot-NBP1-76905-img0006.jpg "Western Blot: RIMS2 Antibody [NBP1-76905] - Analysis in human brain tissue lysate with antibody at 1 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: RIMS2 Antibody - BSA Free [NBP1-76905] - Immunofluorescence of RIMS2 in Human Brain tissue with RIMS2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76905_rabbit-polyclonal-rims2-antibody-2711202419251537.jpg)
![Immunohistochemistry: RIMS2 Antibody - BSA Free [NBP1-76905] - Immunohistochemistry of RIMS2 in human brain with RIMS2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76905_rabbit-polyclonal-rims2-antibody-2711202419451211.jpg)
![Western Blot: FTO Antibody [NBP1-77021] - Analysis of FTO in human uterus tissue lysate with FTO antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/FTO-Antibody-Western-Blot-NBP1-77021-img0004.jpg "Western Blot: FTO Antibody [NBP1-77021] - Analysis of FTO in human uterus tissue lysate with FTO antibody at 1 ug/mL.")
![Immunohistochemistry: FTO Antibody - BSA Free [NBP1-77021] - Immunohistochemistry of FTO in mouse brain tissue with FTO antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77021_rabbit-polyclonal-fto-antibody-2711202419451216.jpg)
![Immunocytochemistry/ Immunofluorescence: FTO Antibody - BSA Free [NBP1-77021] - Immunofluorescence of FTO in Mouse Brain cells with FTO antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77021_rabbit-polyclonal-fto-antibody-2711202420285821.jpg)
![Western Blot: Kv7.1 Antibody [NBP1-30119] - Analysis of Kv7.1 in Human Heart lysate (35ug protein in RIPA buffer) using NBP1-30119 at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Kv7.1-Antibody-Western-Blot-NBP1-30119-img0002.jpg "Western Blot: Kv7.1 Antibody [NBP1-30119] - Analysis of Kv7.1 in Human Heart lysate (35ug protein in RIPA buffer) using NBP1-30119 at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry: Kv7.1 Antibody [NBP1-30119] - (8ug/ml) staining of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.](http://images.novusbio.com/fullsize/Kv7.1-Antibody-Immunohistochemistry-NBP1-30119-img0004.jpg)
![Control: Kv7.1 Antibody [NBP1-30119] - Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.](http://images.novusbio.com/fullsize/Kv7.1-Antibody-Control-NBP1-30119-img0003.jpg)
![Western Blot: KCNN2 Antibody [NBP1-46119] - Staining of human liver lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP1-46119-img0002.jpg "Western Blot: KCNN2 Antibody [NBP1-46119] - Staining of human liver lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: KCNN2 Antibody [NBP1-46119] - Staining of paraffin embedded human kidney. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/KCNN2-Antibody-Immunohistochemistry-Paraffin-NBP1-46119-img0005.jpg)
![Immunohistochemistry-Paraffin: KCNN2 Antibody [NBP1-46119] - Staining of paraffin embedded human thyroid gland. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/KCNN2-Antibody-Immunohistochemistry-Paraffin-NBP1-46119-img0001.jpg)
![Western Blot: NKCC2/SLC12A1 Antibody [NBP1-57622] - Sample Tissue: Human A549 Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Western-Blot-NBP1-57622-img0007.jpg "Western Blot: NKCC2/SLC12A1 Antibody [NBP1-57622] - Sample Tissue: Human A549 Antibody Dilution: 1.0 ug/ml")
![Immunohistochemistry: NKCC2/SLC12A1 Antibody [NBP1-57622] - Paraffin Embedded Tissue: Human Kidney Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0 - 8.0 ug/ml Magnification: 400X](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-NBP1-57622-img0006.jpg)
![Western Blot: NKCC2/SLC12A1 Antibody [NBP1-57622] - Jurkat cell lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Western-Blot-NBP1-57622-img0002.jpg)
![Western Blot: NKCC2/SLC12A1 Antibody [NBP1-57622] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Western-Blot-NBP1-57622-img0005.jpg)
![Western Blot: GAD2/GAD65 Antibody [NB100-1506] - (0.5ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Western-Blot-NB100-1506-img0001.jpg "Western Blot: GAD2/GAD65 Antibody [NB100-1506] - (0.5ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NB100-1506] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.](http://images.novusbio.com/fullsize/nb100-1506_goat-polyclonal-gad2-gad65-antibody-16520239183338.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NB100-1506] - (6ug/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nb100-1506_goat-polyclonal-gad2-gad65-antibody-1652023922513.jpg)
![Immunohistochemistry-Paraffin: Secretagogin Antibody (9K8H9) [NBP3-15814] - Immunohistochemistry of paraffin-embedded mouse stomach using Secretagogin (Secretagogin) Rabbit mAb (NBP3-15814) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-9K8H9-Immunohistochemistry-Paraffin-NBP3-15814-img0003.jpg "Immunohistochemistry-Paraffin: Secretagogin Antibody (9K8H9) [NBP3-15814] - Immunohistochemistry of paraffin-embedded mouse stomach using Secretagogin (Secretagogin) Rabbit mAb (NBP3-15814) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: Secretagogin Antibody (9K8H9) [NBP3-15814] - Immunohistochemistry of paraffin-embedded rat pancreatic islet using Secretagogin (Secretagogin) Rabbit mAb (NBP3-15814) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-9K8H9-Immunohistochemistry-Paraffin-NBP3-15814-img0001.jpg)
![Immunohistochemistry-Paraffin: Secretagogin Antibody (9K8H9) [NBP3-15814] - Immunohistochemistry of paraffin-embedded human colon using Secretagogin (Secretagogin) Rabbit mAb (NBP3-15814) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-9K8H9-Immunohistochemistry-Paraffin-NBP3-15814-img0002.jpg)
![Western Blot: Secretagogin Antibody (9K8H9) [Secretagogin] - Western blot analysis of various lysates using Secretagogin (Secretagogin) Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-15814_rabbit-secretagogin-mab-9k8h9-52202517172898.jpg)
![Western Blot: KCNJ15 Antibody [NBP3-04863] - Analysis of extracts of various cell lines, using KCNJ15 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basi](http://images.novusbio.com/fullsize/KCNJ15-Antibody-Western-Blot-NBP3-04863-img0004.jpg "Western Blot: KCNJ15 Antibody [NBP3-04863] - Analysis of extracts of various cell lines, using KCNJ15 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basi")
![Immunohistochemistry-Paraffin: KCNJ15 Antibody [NBP3-04863] - Mouse kidney using KCNJ15 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KCNJ15-Antibody-Immunohistochemistry-Paraffin-NBP3-04863-img0003.jpg)
![Immunohistochemistry-Paraffin: KCNJ15 Antibody [NBP3-04863] - Human breast cancer using KCNJ15 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KCNJ15-Antibody-Immunohistochemistry-Paraffin-NBP3-04863-img0001.jpg)
![Immunohistochemistry-Paraffin: KCNJ15 Antibody [NBP3-04863] - Rat heart using KCNJ15 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KCNJ15-Antibody-Immunohistochemistry-Paraffin-NBP3-04863-img0002.jpg)
![Western Blot: USP21 Antibody - Azide and BSA Free [USP21] - Western blot analysis of lysates from Mouse brain, using USP21 Rabbit pAb at 1:700 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-03947_rabbit-polyclonal-usp21-antibody-5220251638021.jpg "Western Blot: USP21 Antibody - Azide and BSA Free [USP21] - Western blot analysis of lysates from Mouse brain, using USP21 Rabbit pAb at 1:700 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: USP21 Antibody - Azide and BSA Free [USP21] - Immunofluorescence analysis of PC-12 cells using USP21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03947_rabbit-polyclonal-usp21-antibody-52202516293926.jpg)
![Immunocytochemistry/ Immunofluorescence: USP21 Antibody - Azide and BSA Free [USP21] - Immunofluorescence analysis of HeLa cells using USP21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03947_rabbit-polyclonal-usp21-antibody-522025162802.jpg)
![Immunocytochemistry/ Immunofluorescence: USP21 Antibody - Azide and BSA Free [USP21] - Immunofluorescence analysis of RAW264.7 cells using USP21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03947_rabbit-polyclonal-usp21-antibody-5220251628058.jpg)
![Western Blot: Somatostatin R5/SSTR5 Antibody (4O6N5) [NBP3-16682] - Analysis of extracts of various cell lines, using Somatostatin R5/SSTR5 Rabbit mAb (NBP3-16682) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Somatostatin-R5-SSTR5-Antibody-4O6N5-Western-Blot-NBP3-16682-img0004.jpg "Western Blot: Somatostatin R5/SSTR5 Antibody (4O6N5) [NBP3-16682] - Analysis of extracts of various cell lines, using Somatostatin R5/SSTR5 Rabbit mAb (NBP3-16682) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunohistochemistry: Somatostatin R5/SSTR5 Antibody (4O6N5) [NBP3-16682] - Immunohistochemistry analysis of Somatostatin R5/SSTR5 in paraffin-embedded human liver cancer tissue using Somatostatin R5/SSTR5 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16682_rabbit-somatostatin-r5-sstr5-mab-4o6n5-822025208477.jpg)
![Immunohistochemistry: Somatostatin R5/SSTR5 Antibody (4O6N5) [NBP3-16682] - Immunohistochemistry analysis of Somatostatin R5/SSTR5 in paraffin-embedded rat spleen tissue using Somatostatin R5/SSTR5 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16682_rabbit-somatostatin-r5-sstr5-mab-4o6n5-822025208488.jpg)
![Immunohistochemistry: Somatostatin R5/SSTR5 Antibody (4O6N5) [NBP3-16682] - Immunohistochemistry analysis of Somatostatin R5/SSTR5 in paraffin-embedded mouse testis tissue using Somatostatin R5/SSTR5 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16682_rabbit-somatostatin-r5-sstr5-mab-4o6n5-822025208476.jpg)
![Western Blot: VGCNL1 Antibody (S187-7) [NBP2-22410] - Western Blot analysis of Rat Brain showing detection of ~200 kDa VGCNL1protein using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Lane 1: Molecular Weight (MW) Ladder. Lane 3: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.](http://images.novusbio.com/fullsize/VGCNL1-Antibody-S187-7-Western-Blot-NBP2-22410-img0002.jpg "Western Blot: VGCNL1 Antibody (S187-7) [NBP2-22410] - Western Blot analysis of Rat Brain showing detection of ~200 kDa VGCNL1protein using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Lane 1: Molecular Weight (MW) Ladder. Lane 3: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.")
![Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGCNL1Antibody (D) Composite.](http://images.novusbio.com/fullsize/VGCNL1-Antibody-S187-7-Immunocytochemistry-Immunofluorescence-NBP2-22410-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/VGCNL1-Antibody-S187-7-Immunocytochemistry-Immunofluorescence-NBP2-22410-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VGCNL1Antibody. (D) Composite.](http://images.novusbio.com/fullsize/VGCNL1-Antibody-S187-7-Immunocytochemistry-Immunofluorescence-NBP2-22410-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin Brain Antibody (D) Composite.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0003.jpg "Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin Brain Antibody (D) Composite.")
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin B Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0004.jpg)