| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76677SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70334 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-11267-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-216-0.125ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81021-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1229-0.025ml | |||
![Western Blot: Presenilin-2 Antibody (APS 21) [NB100-74512] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and PC12 (lane 3).](http://images.novusbio.com/fullsize/Presenilin-2-Antibody-APS-21-Western-Blot-NB100-74512-img0009.jpg "Western Blot: Presenilin-2 Antibody (APS 21) [NB100-74512] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and PC12 (lane 3).")
![Immunocytochemistry/Immunofluorescence: Presenilin-2 Antibody (APS 21) [NB100-74512] - Presenilin- 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 2 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Presenilin-2-Antibody-APS-21-Immunocytochemistry-Immunofluorescence-NB100-74512-img0007.jpg)
![Immunohistochemistry-Paraffin: Presenilin-2 Antibody (APS 21) [NB100-74512] - Normal biopsies of deparaffinized Human kidney tissue.](http://images.novusbio.com/fullsize/Presenilin-2-Antibody-APS-21-Immunohistochemistry-Paraffin-NB100-74512-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Presenilin-2 Antibody (APS 21) [NB100-74512] - Presenilin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin- 2 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Presenilin-2-Antibody-APS-21-Immunocytochemistry-Immunofluorescence-NB100-74512-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Presenilin-2 Antibody (APS 21) [NB100-74512] - Presenilin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin- 2 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Presenilin-2-Antibody-APS-21-Immunocytochemistry-Immunofluorescence-NB100-74512-img0006.jpg)
![Western Blot: Nudel Antibody [NBP1-76677] - Jurkat whole cell lysate with Nudel antibody at (A) 0.5, (B) 1, or (C) 2 ug/ml.](http://images.novusbio.com/fullsize/Nudel-Antibody-Western-Blot-NBP1-76677-img0001.jpg "Western Blot: Nudel Antibody [NBP1-76677] - Jurkat whole cell lysate with Nudel antibody at (A) 0.5, (B) 1, or (C) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Nudel Antibody [NBP1-76677] - Immunofluorescence of Nudel in Jurkat cells with Nudel antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/Nudel-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76677-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nudel Antibody [NBP1-76677] - Jurkat cells with Nudel antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Nudel-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76677-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Nudel Antibody - BSA Free [NBP1-76677] - Immunofluorescence of Nudel in Jurkat cells with Nudel antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-76677_rabbit-polyclonal-nudel-antibody-2711202420195390.jpg)
![Immunocytochemistry/ Immunofluorescence: Nudel Antibody - BSA Free [NBP1-76677] - Immunocytochemistry of Nudel in Jurkat cells with Nudel antibody at 2 u/mL.](http://images.novusbio.com/fullsize/nbp1-76677_rabbit-polyclonal-nudel-antibody-271120241928851.jpg)
![Immunocytochemistry/ Immunofluorescence: Nudel Antibody - BSA Free [NBP1-76677] - Immunofluorescence of Nudel in Jurkat cells with Nudel antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-76677_rabbit-polyclonal-nudel-antibody-2711202420392740.jpg)
![Immunocytochemistry/ Immunofluorescence: Nudel Antibody - BSA Free [NBP1-76677] - Immunocytochemistry of Nudel in Jurkat cells with Nudel antibody at 2 u/mL.](http://images.novusbio.com/fullsize/nbp1-76677_rabbit-polyclonal-nudel-antibody-2711202420324811.jpg)
![Western Blot: Calpain 2 Antibody (3G1) [NBP2-46063] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-3G1-Western-Blot-NBP2-46063-img0005.jpg "Western Blot: Calpain 2 Antibody (3G1) [NBP2-46063] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2.")
![Immunohistochemistry: Calpain 2 Antibody (3G1) [NBP2-46063] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-3G1-Immunohistochemistry-NBP2-46063-img0004.jpg)
![Immunohistochemistry: Calpain 2 Antibody (3G1) [NBP2-46063] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-3G1-Immunohistochemistry-NBP2-46063-img0001.jpg)
![Immunohistochemistry: Calpain 2 Antibody (3G1) [NBP2-46063] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-3G1-Immunohistochemistry-NBP2-46063-img0002.jpg)
![Immunohistochemistry: Calpain 2 Antibody (3G1) [NBP2-46063] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-3G1-Immunohistochemistry-NBP2-46063-img0003.jpg)
![Western Blot: Synapsin I [p Ser603] Antibody [NB300-181] - The figure depicts Western blots of the two synaptic plasticity markers investigated. The two bands seen in the p-synapsin and synapsin Western blots (70 and 74 kDa respectively) are consistent with splice variants. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/10/6/749) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Synapsin-I-[p-Ser603]-Antibody-Western-Blot-NB300-181-img0002.jpg "Western Blot: Synapsin I [p Ser603] Antibody [NB300-181] - The figure depicts Western blots of the two synaptic plasticity markers investigated. The two bands seen in the p-synapsin and synapsin Western blots (70 and 74 kDa respectively) are consistent with splice variants. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/10/6/749) licensed under a CC-BY license.")
![Western Blot: Synapsin I [p Ser603] Antibody [NB300-181] - Rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser603 in the first lane (-). Phosphospecificity is shown in thesecond lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (l-Ptase, 1200 units for 30 minutes).](http://images.novusbio.com/fullsize/Synapsin-I-[p-Ser603]-Antibody-Western-Blot-NB300-181-img0001.jpg)
![Western Blot: Synapsin I [p Ser603] Antibody [NB300-181] - Examples of Western blots of p-/synapsin 1 & p-/Ca2+-calmodulin-dependent kinase II. The figure depicts examples of the Western blots of the four synaptic plasticity markers investigated. The two bands seen in the p-synapsin & synapsin Western blots (70 & 74 kDa respectively) are consistent with splice variants as confirmed by manufacturer. P-CAMKII & CAMKII were detected around 50 kDa in accordance with previous findings. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29890692), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-181_rabbit-polyclonal-synapsin-i-p-ser603-antibody-imgenex-img-683-31020241539736.jpg)
![Western Blot: MAP2 Antibody (HM-2) [NB120-11267] - Rat brain extract (adult and newborn) was separated on SDS-PAGE and probed with Monoclonal PA To MAP2 (MO) CL:HM-2 . The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBTBCIP substrate. Observed molecular weight is 280 kDA.](http://images.novusbio.com/fullsize/MAP2-Antibody-HM-2-Western-Blot-NB120-11267-img0003.jpg "Western Blot: MAP2 Antibody (HM-2) [NB120-11267] - Rat brain extract (adult and newborn) was separated on SDS-PAGE and probed with Monoclonal PA To MAP2 (MO) CL:HM-2 . The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBTBCIP substrate. Observed molecular weight is 280 kDA.")
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (HM-2) [NB120-11267] - B35 cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-HM-2-Immunocytochemistry-Immunofluorescence-NB120-11267-img0002.jpg)
![Western Blot: Tau Antibody [NBP2-25163] - Analysis of tissue lysates using chicken pAb to MAP-tau, NBP2-25163, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as multiple isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot in the range from 48 kDa to 67 kDa.](http://images.novusbio.com/fullsize/Tau-Antibody-Western-Blot-NBP2-25163-img0005.jpg "Western Blot: Tau Antibody [NBP2-25163] - Analysis of tissue lysates using chicken pAb to MAP-tau, NBP2-25163, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as multiple isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot in the range from 48 kDa to 67 kDa.")
![Immunocytochemistry/Immunofluorescence: Tau Antibody [NBP2-25163] - Immunofluorescent analysis of cortical neuron-glial culture from E20 rat stained with chicken pAb to MAP-tau, NBP2-25163, dilution 1:2,000 in green, and costained with mouse mAb to MAP2, dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. The NBP2-25163 antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both MAP2 and tau proteins.](http://images.novusbio.com/fullsize/Tau-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25163-img0004.jpg)
![Western Blot: alpha-Internexin Antibody (1D2) [NB300-216] - Analysis of different tissue lysates using alpha-Internexin antibody, dilution 1:10,000 (Green): [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord and [7] cow spinal cord. The alpha-Internexin antibody reveals the alpha-internexin protein with apparent molecular weight of 64 to 66 kDa with slight variability among species.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-1D2-Western-Blot-NB300-216-img0002.jpg "Western Blot: alpha-Internexin Antibody (1D2) [NB300-216] - Analysis of different tissue lysates using alpha-Internexin antibody, dilution 1:10,000 (Green): [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord and [7] cow spinal cord. The alpha-Internexin antibody reveals the alpha-internexin protein with apparent molecular weight of 64 to 66 kDa with slight variability among species.")
![Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody (1D2) [NB300-216] - Mixed cultures of rat CNS cells stained with NB300-216 (red) and chicken antibody to Microtubule associated protein 2 (MAP2- green). The a-internexin is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-1D2-Immunocytochemistry-Immunofluorescence-NB300-216-img0001.jpg)
![Immunohistochemistry Free-Floating: alpha-Internexin Antibody (1D2) [NB300-216] - Analysis of rat cerebellum section stained with alpha-internexin antibody, dilution 1:5,000 (Green), and costained with chicken Calretinin pAb, dilution 1:2,000 (Red). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with the above antibodies. The alpha-internexin antibody selectively stains neuronal processes, in particular parallel fibers, the axons of granule cells. Calretinin antibody stains interneurons predominantly in the molecular layer of the cerebellum.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-1D2-Immunohistochemistry-Free-Floating-NB300-216-img0003.jpg)
![Western Blot: QKI/Quaking Antibody [NB300-240] - Detection of Human and Mouse QKI by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-QKI antibody NB300-240 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NB300-240-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: QKI/Quaking Antibody [NB300-240] - Detection of Human and Mouse QKI by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-QKI antibody NB300-240 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: QKI/Quaking Antibody [NB300-240] - Sample: FFPE sections of human testicular seminoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- QKI used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-NB300-240-img0006.jpg)
![Immunoprecipitation: QKI/Quaking Antibody [NB300-240] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. antibodies: Affinity purified rabbit anti-QKI antibody used for IP at 6 ug/mg lysate. QKI was also immunoprecipitated by a prior lot of this antibody. For blotting immunoprecipitated QKI, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunoprecipitation-NB300-240-img0011.jpg)
![Western Blot: EFHD2 Antibody [NBP1-31134] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EFHD2 antibody (NBP1-31134). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EFHD2-Antibody-Western-Blot-NBP1-31134-img0006.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: EFHD2 Antibody [NBP1-31134] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EFHD2 antibody (NBP1-31134). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: EFHD2 Antibody [NBP1-31134] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EFHD2 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/EFHD2-Antibody-Western-Blot-NBP1-31134-img0003.jpg)
![Western Blot: EFHD2 Antibody [NBP1-31134] - Sample(30 ug of whole cell lysate)A:H129910% SDS PAGENBP1-31134 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-31134_rabbit-polyclonal-efhd2-antibody-91020242011492.jpg)
![EFHD2 antibody detects EFHD2 protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: EFHD2 protein stained by EFHD2 antibody (NBP1-31134) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31134_rabbit-polyclonal-efhd2-antibody-immunocytochemistry-immunofluorescence-141120251840164.jpg)
![EFHD2 antibody detects EFHD2 protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: EFHD2 protein stained by EFHD2 antibody (NBP1-31134) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48818_rabbit-gat-1-slc6a1-mab-hl3475-immunohistochemistry-frozen-14112025185549.jpg)
![Western Blot: Synapsin II Antibody [NBP3-13445] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody (NBP3-13445) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Synapsin-II-Antibody-Western-Blot-NBP3-13445-img0002.jpg "Western Blot: Synapsin II Antibody [NBP3-13445] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody (NBP3-13445) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Synapsin II Antibody [NBP3-13445] - Rat brain. Synapsin II stained by Synapsin II antibody (NBP3-13445) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Synapsin-II-Antibody-Immunohistochemistry-Paraffin-NBP3-13445-img0005.jpg)
![Western Blot: Synapsin II Antibody [NBP3-13445] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody (NBP3-13445) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Synapsin-II-Antibody-Western-Blot-NBP3-13445-img0001.jpg)
![Immunohistochemistry-Paraffin: Synapsin II Antibody [NBP3-13445] - Mouse brain. Synapsin II stained by Synapsin II antibody (NBP3-13445) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Synapsin-II-Antibody-Immunohistochemistry-Paraffin-NBP3-13445-img0004.jpg)
![Synapsin II antibody detects Synapsin II protein at Synapse by immunofluorescent analysis.Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Synapsin II stained by Synapsin II antibody (NBP3-13445) diluted at 1:500.Red: Tau, a marker, stained by Tau antibody [GT287] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp3-13445_rabbit-synapsin-ii-pab-immunocytochemistry-immunofluorescence-2611202518283516.jpg)
![Immunocytochemistry/Immunofluorescence: Myt1 Antibody [NBP1-81021] - Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/Myt1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81021-img0006.jpg "Immunocytochemistry/Immunofluorescence: Myt1 Antibody [NBP1-81021] - Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry-Paraffin: Myt1 Antibody [NBP1-81021] - Staining of human cerebellum shows moderate nuclear positivity in cells in granular layer.](http://images.novusbio.com/fullsize/Myt1-Antibody-Immunohistochemistry-Paraffin-NBP1-81021-img0005.jpg)
![Western Blot: DLG1 Antibody [NB600-1229] - Analysis of 25 ug of C6 (lane 1), PC12 (lane 2) and rat brain (lane 3) cell lysates.](http://images.novusbio.com/fullsize/DLG1-Antibody-Western-Blot-NB600-1229-img0003.jpg "Western Blot: DLG1 Antibody [NB600-1229] - Analysis of 25 ug of C6 (lane 1), PC12 (lane 2) and rat brain (lane 3) cell lysates.")
![Immunocytochemistry/Immunofluorescence: DLG1 Antibody [NB600-1229] - Analysis of SAP97 (green) showing staining in the cytoplasm and membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a SAP97 polyclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/fullsize/DLG1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1229-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DLG1 Antibody [NB600-1229] - Analysis of SAP97 (green) showing staining in the cytoplasm and membrane of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a SAP97 polyclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/fullsize/DLG1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1229-img0001.jpg)