| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59359-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24781SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33104 | |||
| IHC-Prediluted | NBP2-44839 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44853 | |||
| Azide and BSA Free | NBP2-34527-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47969-0.1mg | |||
| IHC-Prediluted | NBP2-48292 | |||
![Western Blot: Acrolein Antibody (10A10) [NBP2-59359] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10 (NBP2-59359). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59359) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.](http://images.novusbio.com/fullsize/Acrolein-Antibody-10A10-Western-Blot-NBP2-59359-img0012.jpg "Western Blot: Acrolein Antibody (10A10) [NBP2-59359] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10 (NBP2-59359). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59359) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.")
![Immunocytochemistry/Immunofluorescence: Acrolein Antibody (10A10) [NBP2-59359] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/Acrolein-Antibody-10A10-Immunocytochemistry-Immunofluorescence-NBP2-59359-img0002.jpg)
![Immunohistochemistry: Acrolein Antibody (10A10) [NBP2-59359] - Immunohistochemistry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Tissue: Adrenal Carcinoma. Species: Human. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.](http://images.novusbio.com/fullsize/Acrolein-Antibody-10A10-Immunohistochemistry-NBP2-59359-img0010.jpg)
![Flow Cytometry: Acrolein Antibody (10A10) [NBP2-59359] - Flow Cytometry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10 (NBP2-59359). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59359) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 M H2O2 for 24 hours.](http://images.novusbio.com/fullsize/Acrolein-Antibody-10A10-Flow-Cytometry-NBP2-59359-img0013.jpg)
![Western Blot: Acrolein Antibody (10A10) [NBP2-59359] - Western Blot analysis of Acrolein-BSA Conjugate showing detection of 67 kDa Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10 (NBP2-59359). Lane 1: Molecular Weight Ladder (MW). Lane 2: AcroleinBSA (0.5 ug). Lane 3: AcroleinBSA (2.0 ug). Lane 4: BSA (0.5 ug). Lane 5: BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59359) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.](http://images.novusbio.com/fullsize/Acrolein-Antibody-10A10-Western-Blot-NBP2-59359-img0011.jpg)
![Western Blot: Tmp21/p23 Antibody (PSH01-41) [NBP3-33031] - Western blot analysis of Tmp21/p23 on different lysates with Rabbit anti-Tmp21/p23 antibody (NBP3-33031) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: Raji cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: U-87 MG cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse pancreas tissue lysate (40 ug/Lane) Lane 9: Rat pancreas tissue lysate (40 ug/Lane) Lane 10: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 25 kDa Observed band size: 19 kDa Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33031) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33031_rabbit-tmp21-p23-mab-psh01-41-288202412493461.jpg "Western Blot: Tmp21/p23 Antibody (PSH01-41) [NBP3-33031] - Western blot analysis of Tmp21/p23 on different lysates with Rabbit anti-Tmp21/p23 antibody (NBP3-33031) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: Raji cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: U-87 MG cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse pancreas tissue lysate (40 ug/Lane) Lane 9: Rat pancreas tissue lysate (40 ug/Lane) Lane 10: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 25 kDa Observed band size: 19 kDa Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33031) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tmp21/p23 Antibody (PSH01-41) [NBP3-33031] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Tmp21/p23 with Rabbit anti-Tmp21/p23 antibody (NBP3-33031) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33031, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33031_rabbit-tmp21-p23-mab-psh01-41-298202414161859.jpg)
![Immunohistochemistry: Tmp21/p23 Antibody (PSH01-41) [NBP3-33031] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Tmp21/p23 antibody (NBP3-33031) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33031) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33031_rabbit-tmp21-p23-mab-psh01-41-298202413585570.jpg)
![Immunocytochemistry/ Immunofluorescence: Tmp21/p23 Antibody (PSH01-41) [NBP3-33031] - Immunocytochemistry analysis of HeLa cells labeling Tmp21/p23 with Rabbit anti-Tmp21/p23 antibody (NBP3-33031) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Tmp21/p23 antibody (NBP3-33031) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33031_rabbit-tmp21-p23-mab-psh01-41-29202410234260.jpg)
![Flow Cytometry: Tmp21/p23 Antibody (PSH01-41) [NBP3-33031] - Flow cytometric analysis of HeLa cells labeling Tmp21/p23. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33031, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33031_rabbit-tmp21-p23-mab-psh01-41-29202416453210.jpg)
![Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of Huntingtin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: A431 cell lysate](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Western-Blot-NBP2-75531-img0006.jpg "Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of Huntingtin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: A431 cell lysate")
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded rat brain tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0005.jpg)
![Flow Cytometry: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of SH-SY-5Y cells with Huntingtin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Flow-Cytometry-NBP2-75531-img0001.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded human colon cancer tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0002.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded human breast tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0003.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded mouse epididymis tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (JF10-30) [NBP2-66994] - Staining C3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-JF10-30-Immunocytochemistry-Immunofluorescence-NBP2-66994-img0002.jpg "Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (JF10-30) [NBP2-66994] - Staining C3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.")
![Flow Cytometry: Complement C3 Antibody (JF10-30) [NBP2-66994] - Analysis of HepG2 cells with C3 antibody at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Complement-C3-Antibody-JF10-30-Flow-Cytometry-NBP2-66994-img0001.jpg)
![Flow Cytometry: Complement C3 Antibody (JF10-30) [NBP2-66994] - Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4c for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4 c. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-66994_rabbit-monoclonal-complement-c3-antibody-jf10-30-flow-cytometry-1542024105623..jpg)
![Western Blot: Complement C3 Antibody (JF10-30) [NBP2-66994] -Western blot analysis of C3 on different lysates with Rabbit anti-C3 antibody at 1/1,000 dilution.Lane 1: Human liver tissue lysateLane 2: HepG2 cell lysateLane 3: HeLa cell lysateLane 4: Mouse liver tissue lysateLane 5: Mouse spleen tissue lysateLane 6: Rat liver tissue lysateLane 7: Rat spleen tissue lysateLysates/proteins at 30 µg/Lane.Predicted band size: 187 kDaObserved band size: 108/37 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.](http://images.novusbio.com/fullsize/nbp2-66994_rabbit-monoclonal-complement-c3-antibody-jf10-30-western-blot-1542024105739..jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (JF10-30) [NBP2-66994] -Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-C3 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4 c. Goat Anti-Mouse IgG H&L (iFluor™ ) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-66994_rabbit-monoclonal-complement-c3-antibody-jf10-30-immunocytochemistry-immunofluorescence-15420241102..jpg)
![Western Blot: A20/TNFAIP3 Antibody (SN07-31) [NBP2-67676] - Western blot analysis of A20/TNFAIP3 on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-SN07-31-Western-Blot-NBP2-67676-img0007.jpg "Western Blot: A20/TNFAIP3 Antibody (SN07-31) [NBP2-67676] - Western blot analysis of A20/TNFAIP3 on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat")
![Immunocytochemistry/Immunofluorescence: A20/TNFAIP3 Antibody (SN07-31) [NBP2-67676] - Staining TNFAIP3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-SN07-31-Immunocytochemistry-Immunofluorescence-NBP2-67676-img0004.jpg)
![Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody (SN07-31) [NBP2-67676] - Analysis of paraffin-embedded human kidney tissue using anti-TNFAIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-SN07-31-Immunohistochemistry-Paraffin-NBP2-67676-img0005.jpg)
![Flow Cytometry: A20/TNFAIP3 Antibody (SN07-31) [NBP2-67676] - Analysis of HepG2 cells with TNFAIP3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-SN07-31-Flow-Cytometry-NBP2-67676-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: A20/TNFAIP3 Antibody (SN07-31) [NBP2-67676] - Staining TNFAIP3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-SN07-31-Immunocytochemistry-Immunofluorescence-NBP2-67676-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: A20/TNFAIP3 Antibody (SN07-31) [NBP2-67676] - Staining TNFAIP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-SN07-31-Immunocytochemistry-Immunofluorescence-NBP2-67676-img0003.jpg)
![Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Western-Blot-NBP2-67373-img0006.jpg "Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0003.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67373-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of paraffin-embedded human kidney tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67373-img0004.jpg)
![Western Blot: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Analysis of MTCO2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysateLane 2: THP-1 cell lysate Lane 3: Hela cell lysate Lane 4: human lung tissue lysate Lane 5: human placenta tissue lysate](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Western-Blot-NBP2-67617-img0006.jpg "Western Blot: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Analysis of MTCO2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysateLane 2: THP-1 cell lysate Lane 3: Hela cell lysate Lane 4: human lung tissue lysate Lane 5: human placenta tissue lysate")
![Immunocytochemistry/Immunofluorescence: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Staining MTCO2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Immunocytochemistry-Immunofluorescence-NBP2-67617-img0004.jpg)
![Immunohistochemistry-Paraffin: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Analysis of paraffin-embedded human liver tissue using anti-MTCO2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Immunohistochemistry-Paraffin-NBP2-67617-img0005.jpg)
![Flow Cytometry: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Analysis of HepG2 cells with MTCO2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Flow-Cytometry-NBP2-67617-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Staining MTCO2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Immunocytochemistry-Immunofluorescence-NBP2-67617-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Staining MTCO2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Immunocytochemistry-Immunofluorescence-NBP2-67617-img0003.jpg)
![Western Blot: PGP9.5 / UCHL-1 Antibody [NBP2-24781] - Western blot analysis of PGP9.5 / UCHL-1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using PGP9.5 / UCHL-1 antibody at 0.2 ug/ml.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NBP2-24781-img0005.jpg "Western Blot: PGP9.5 / UCHL-1 Antibody [NBP2-24781] - Western blot analysis of PGP9.5 / UCHL-1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using PGP9.5 / UCHL-1 antibody at 0.2 ug/ml.")
![Immunohistochemistry-Paraffin: PGP9.5 / UCHL-1 Antibody [NBP2-24781] - IHC analysis of human brain, cortex, using PGP9.5 / UCHL-1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunohistochemistry-Paraffin-NBP2-24781-img0003.jpg)
![Immunohistochemistry-Paraffin: CD45RO Antibody (UCHL-1) [NBP2-29631] - Staining of human tonsil](http://images.novusbio.com/fullsize/CD45RO-Antibody-UCHL-1-Immunohistochemistry-Paraffin-NBP2-29631-img0004.jpg "Immunohistochemistry-Paraffin: CD45RO Antibody (UCHL-1) [NBP2-29631] - Staining of human tonsil")
![<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: CD45RO Antibody (UCHL-1) [NBP2-29631] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD45 mRNA (ACD RNAScope probe, catalog # 601991; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using mouse monoclonal (Novus catalog # NBP2-29631) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC001) and green HRP chromogen (ACD Catalog # 322500).](http://images.novusbio.com/fullsize/CD45RO-Antibody-UCHL-1-Dual-RNAscope-ISH-IHC-NBP2-29631-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CD45RO Antibody (UCHL-1) [NBP2-29631] - Immunofluorescence Analysis of human tonsil FFPE section stained with CD45RO antibody (UCHL-1)and goat anti-mouse HRP, detected using CF488A tyramide in Tyramide Amplification Buffer Plus.](http://images.novusbio.com/fullsize/CD45RO-Antibody-UCHL-1-Immunocytochemistry-Immunofluorescence-NBP2-29631-img0008.jpg)
![Flow Cytometry: CD45RO Antibody (UCHL-1) [NBP2-29631] - Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45RO Antibody (UCHL-1) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD45RO-Antibody-UCHL-1-Flow-Cytometry-NBP2-29631-img0007.jpg)
![Flow Cytometry: CD45RO Antibody (UCHL-1) [NBP2-29631] - CD45RO expression by lymphocyte gated population of PBMC: PBMC were stained either with purified CD45RO antibody (0.5 ug/10^6 cells) or isotype control following surface staining protocol. PE-conjugated goat anti-mouse IgG, secondary antibody used to identify the positive staining on PPI negative lymphocyte gated population.](http://images.novusbio.com/fullsize/CD45RO-Antibody-UCHL-1-Flow-Cytometry-NBP2-29631-img0002.jpg)
![Formalin-fixed, paraffin-embedded human tonsil stained with CD45 antibody (2B11) [NBP2-32934].](http://images.novusbio.com/fullsize/CD45-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-32934-img0002.jpg "Formalin-fixed, paraffin-embedded human tonsil stained with CD45 antibody (2B11) [NBP2-32934].")
![Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD45 antibody (2B11) [NBP2-32934].](http://images.novusbio.com/fullsize/CD45-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-32934-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (NR-4) [NBP2-44891] - Immunofluorescence staining of HEK293 cells using NF-L Antibody (NR-4) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NR-4-Immunocytochemistry-Immunofluorescence-NBP2-44891-img0005.jpg "Immunocytochemistry/Immunofluorescence: NF-L Antibody (NR-4) [NBP2-44891] - Immunofluorescence staining of HEK293 cells using NF-L Antibody (NR-4) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).")
![Immunohistochemistry-Paraffin: NF-L Antibody (NR-4) [NBP2-44891] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NR-4).](http://images.novusbio.com/fullsize/NF-L-Antibody-NR-4-Immunohistochemistry-Paraffin-NBP2-44891-img0002.jpg)
![Flow Cytometry: NF-L Antibody (NR-4) [NBP2-44891] - Flow Cytometric Analysis of HEK293 cells using NF-L Antibody (NR-4) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NR-4-Flow-Cytometry-NBP2-44891-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (NR-4) [NBP2-44891] - Human Cerebellum stained with Neurofilament Monoclonal Antibody (NR-4).](http://images.novusbio.com/fullsize/NF-L-Antibody-NR-4-Immunohistochemistry-Paraffin-NBP2-44891-img0001.jpg)
![Western Blot: Nrf1 Antibody [NBP1-77822] - Lane: NFR1-HIS recombinant protein. Load: 50ug per lane. Primary antibody - 1: NRF1 antibody at 1:1,000 overnight at 4C. Primary antibody - 2: 6xHIS Epitope tag antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/observed size: 67 kDa, 67 kDa for NRF1-His tagged. Other band(s): None.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-77822-img0003.jpg "Western Blot: Nrf1 Antibody [NBP1-77822] - Lane: NFR1-HIS recombinant protein. Load: 50ug per lane. Primary antibody - 1: NRF1 antibody at 1:1,000 overnight at 4C. Primary antibody - 2: 6xHIS Epitope tag antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/observed size: 67 kDa, 67 kDa for NRF1-His tagged. Other band(s): None.")
![Immunohistochemistry: Nrf1 Antibody [NBP1-77822] - Used at a 5 ug/ml to detect NRF1 in a variety of tissues. This image shows NRF1 staining of human testis. The antibody shows nuclear staining in lymphocytes. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-NBP1-77822-img0004.jpg)
![Western Blot: Nrf1 Antibody [NBP1-77822] - Shows detection of a 67-kDa band corresponding to human NRF1 in a (lane 1) HeLa nuclear extract and (lane 2) whole cell lysate (molecular weight marker not shown). Approx. 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye (TM) 700 labeled Goat-a-Rabbit IgG [H&L] diluted 1:2,500. IRDye (TM) 700](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-77822-img0002.jpg)
