| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47611-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12893-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59359-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72977 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61673-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37525-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94075-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93831-0.02ml | |||
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-294202517153..jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-30420259378..jpg)
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Whole cell and rat tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-304202593920..jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2497) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.Capture: Tau (p Thr181) antibody [HL2498] (NBP3-45158) (5 ug/mL)Detection: HRP-conjugated Tau (p Thr181) antibody [HL2497] (NBP3-45157) (1 ug/mL)Please notice that NBP3-45157 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-45158.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202594949.jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2497) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.Capture: Tau (phospho Thr181) antibody [HL2497] (NBP3-45157) (5 ug/mL)Detection: HRP-conjugated Tau (p Thr181) antibody [HL2498] (NBP3-45158) (1 ug/mL)Please notice that NBP3-45158 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-45157. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202512432..jpg)
![Immunohistochemistry: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Tau (p Thr181) antibody [HL2497] detects Tau (p Thr181) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).Sample: Paraffin-embedded mouse brain.Tau (p Thr181) stained by Tau (p Thr181) antibody [HL2497] (GTX638855) diluted at 1:100.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-immunohistochemistry-3042025122656..jpg)
![Western Blot: Huntingtin Antibody [NBP3-13106] - Non-transfected (-) and transfected (+) U87-MG whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Huntingtin Antibody [NBP3-13106] - Non-transfected (-) and transfected (+) U87-MG whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Huntingtin Antibody [NBP3-13106] - Huntingtin antibody detects Huntingtin protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Huntingtin antibody (NBP3-13106) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Immunohistochemistry-Paraffin-NBP3-13106-img0002.jpg)
![Western Blot: Huntingtin Antibody [NBP3-13106] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0001.jpg)
![Western Blot: Huntingtin Antibody [NBP3-13106] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0004.jpg)
![Western Blot: Huntingtin Antibody [NBP3-13106] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Huntingtin Antibody [NBP3-13106] - Wild-type (WT) and HTT knockout (KO) 293T cell extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13106_rabbit-huntingtin-pab-161020241940576.jpg)
![Western Blot: NMDAR2B Antibody (S59) [NBP1-47611] - Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NMDAR2B protein using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Western-Blot-NBP1-47611-img0008.jpg "Western Blot: NMDAR2B Antibody (S59) [NBP1-47611] - Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NMDAR2B protein using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2B/NMDAR2B Antibody (D) Composite.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Immunocytochemistry-Immunofluorescence-NBP1-47611-img0010.jpg)
![Immunohistochemistry: NMDAR2B Antibody (S59) [NBP1-47611] - Immunohistochemistry analysis using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining, and dermal staining.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Immunohistochemistry-NBP1-47611-img0009.jpg)
![Western Blot: NMDAR2B Antibody (S59) [NBP1-47611] - analysis of Human HEK-T lysates showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36 . Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:250.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Western-Blot-NBP1-47611-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything positive.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Immunocytochemistry-Immunofluorescence-NBP1-47611-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Cav1.3 Antibody (S48) [NBP2-12893] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.3Monoclonal Antibody, Clone S48 (NBP2-12893). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav1.3Monoclonal Antibody (NBP2-12893) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.3Antibody (D) Composite.](http://images.novusbio.com/fullsize/Cav1-3-Antibody-S48-Immunocytochemistry-Immunofluorescence-NBP2-12893-img0007.jpg "Immunocytochemistry/Immunofluorescence: Cav1.3 Antibody (S48) [NBP2-12893] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.3Monoclonal Antibody, Clone S48 (NBP2-12893). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav1.3Monoclonal Antibody (NBP2-12893) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.3Antibody (D) Composite.")
![Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893] - Immunohistochemistry analysis using Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody, Clone S48 (NBP2-12893). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody (NBP2-12893) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Cav1-3-Antibody-S48-Immunohistochemistry-NBP2-12893-img0006.jpg)
![Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893] - IHC analysis of Cav1.3 tested on human hippocampus lysates. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/Cav1-3-Antibody-S48-Immunohistochemistry-NBP2-12893-img0001.jpg)
![Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893] - Immunohistochemistry analysis using Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody, Clone S48 (NBP2-12893). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody (NBP2-12893) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Cav1-3-Antibody-S48-Immunohistochemistry-NBP2-12893-img0005.jpg)
![Western Blot: Acrolein Antibody (10A10) [NBP2-59359] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10 (NBP2-59359). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59359) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.](http://images.novusbio.com/fullsize/Acrolein-Antibody-10A10-Western-Blot-NBP2-59359-img0012.jpg "Western Blot: Acrolein Antibody (10A10) [NBP2-59359] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10 (NBP2-59359). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59359) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.")
![Immunocytochemistry/Immunofluorescence: Acrolein Antibody (10A10) [NBP2-59359] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/Acrolein-Antibody-10A10-Immunocytochemistry-Immunofluorescence-NBP2-59359-img0002.jpg)
![Immunohistochemistry: Acrolein Antibody (10A10) [NBP2-59359] - Immunohistochemistry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Tissue: Adrenal Carcinoma. Species: Human. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.](http://images.novusbio.com/fullsize/Acrolein-Antibody-10A10-Immunohistochemistry-NBP2-59359-img0010.jpg)
![Flow Cytometry: Acrolein Antibody (10A10) [NBP2-59359] - Flow Cytometry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10 (NBP2-59359). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59359) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 M H2O2 for 24 hours.](http://images.novusbio.com/fullsize/Acrolein-Antibody-10A10-Flow-Cytometry-NBP2-59359-img0013.jpg)
![Western Blot: Acrolein Antibody (10A10) [NBP2-59359] - Western Blot analysis of Acrolein-BSA Conjugate showing detection of 67 kDa Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10 (NBP2-59359). Lane 1: Molecular Weight Ladder (MW). Lane 2: AcroleinBSA (0.5 ug). Lane 3: AcroleinBSA (2.0 ug). Lane 4: BSA (0.5 ug). Lane 5: BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59359) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.](http://images.novusbio.com/fullsize/Acrolein-Antibody-10A10-Western-Blot-NBP2-59359-img0011.jpg)
![Western Blot: SIN3A Antibody [NB100-2252] - Detection of Human and Mouse SIN3A Antibody on HeLa whole cell lysate using NB100-2252. SIN3A Antibody was also immunoprecipitated using rabbit anti-SIN3A Antibody NB100-2253, NB100-2254 and another Sin3A antibody.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Western-Blot-NB100-2252-img0005.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SIN3A Antibody [NB100-2252] - Detection of Human and Mouse SIN3A Antibody on HeLa whole cell lysate using NB100-2252. SIN3A Antibody was also immunoprecipitated using rabbit anti-SIN3A Antibody NB100-2253, NB100-2254 and another Sin3A antibody.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: SIN3A Antibody [NB100-2252] - Sample: FFPE sections of human metastatic lymph node (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Sin3A used at a dilution of 1:200 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-NB100-2252-img0013.jpg)
![Immunohistochemistry-Paraffin: SIN3A Antibody [NB100-2252] - FFPE section of human ovarian tumor. Rabbit anti-SIN3A Antibody used at a dilution of 1:250.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NB100-2252-img0011.jpg)
![Immunohistochemistry-Paraffin: SIN3A Antibody [NB100-2252] - FFPE section of mouse squamous cell carcinoma. Rabbit anti-SIN3A Antibody used at a dilution of 1:250.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NB100-2252-img0007.jpg)
![Western Blot: NF-M Antibody (2G3) [NBP2-46139] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NF-M.](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Western-Blot-NBP2-46139-img0010.jpg "Western Blot: NF-M Antibody (2G3) [NBP2-46139] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NF-M.")
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human testicular cancer tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0009.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human skin tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0001.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human adult heart tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0002.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human adult brain tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0003.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human melanoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0004.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human embryonic liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0005.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human muscle tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0006.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human embryonic brain cortex tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0007.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human embryonic cerebellum tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0008.jpg)
![NF-M Antibody (OTI2G3)[NBP2-46139]Simple Western� analysis of lysates (0.2 mg/mL) from HEK293T cell culture using CD63 mouse mAb (SKU: TA803474). EVs were harvested from cell culture media and enriched by polymer-based precipitation. EV contained cell culture media without enrichment was used as controls. The virtual lane view (left) shows the target band corresponding to CD63 (highly glycosylated) at 1:20 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary of EV lysate (Green), cell lysate (Blue) and unenriched media (Grey). The experiment was performed on the Jess Simple Western instrument for ProteinSimple, a BioTechne band, using the 12-230 kDa separation module. *CD63 is highly glycosylated protein, verified by overexpressed lysate tagged with DDK. PNGase F treatment can bring the band to the expected size.](http://images.novusbio.com/fullsize/nbp2-46139_mouse-nf-m-mab-oti2g3-simple-western-131120251236356.jpg%253fstore=america&image-type=image)
![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis using CHRNA3 mAb against human CHRNA3 (AA: 32-240) recombinant protein. (Expected MW is 50.6 kDa)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Western-Blot-NBP2-61673-img0005.jpg "Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis using CHRNA3 mAb against human CHRNA3 (AA: 32-240) recombinant protein. (Expected MW is 50.6 kDa)")
![Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SMMC-7721 cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Immunocytochemistry-Immunofluorescence-NBP2-61673-img0004.jpg)
![Flow Cytometry: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SK-N-SH cells using CHRNA3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Flow-Cytometry-NBP2-61673-img0007.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis using CHRNA3 mAb against HEK293 (1) and CHRNA3 (AA: 32-240)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Western-Blot-NBP2-61673-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of Hela cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Immunocytochemistry-Immunofluorescence-NBP2-61673-img0003.jpg)
![Flow Cytometry: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SH-SY5Y cells using CHRNA3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Flow-Cytometry-NBP2-61673-img0002.jpg)
![ELISA: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-ELISA-NBP2-61673-img0001.jpg)
![Western Blot: NF-L Antibody (2G10) [NBP2-37525] - Analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Western-Blot-NBP2-37525-img0002.jpg "Western Blot: NF-L Antibody (2G10) [NBP2-37525] - Analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (2G10) [NBP2-37525] - Analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Immunocytochemistry-Immunofluorescence-NBP2-37525-img0003.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (2G10) [NBP2-37525] - Analysis of brain tissues using NEFL mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Immunohistochemistry-NBP2-37525-img0006.jpg)
![Flow Cytometry: NF-L Antibody (2G10) [NBP2-37525] - Analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Flow-Cytometry-NBP2-37525-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (2G10) [NBP2-37525] - Analysis of lung cancer tissues using NEFL mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Immunohistochemistry-NBP2-37525-img0005.jpg)
![ELISA: NF-L Antibody (2G10) [NBP2-37525] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-ELISA-NBP2-37525-img0001.jpg)
![Western Blot: TOMM40 Antibody [NBP2-94075] - Analysis of extracts of various cell lines, using TOMM40 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.](http://images.novusbio.com/fullsize/TOMM40-Antibody-Western-Blot-NBP2-94075-img0001.jpg "Western Blot: TOMM40 Antibody [NBP2-94075] - Analysis of extracts of various cell lines, using TOMM40 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: TOMM40 Antibody [NBP2-94075] - Analysis of U2OS cells using TOMM40 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/TOMM40-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94075-img0002.jpg)
![Western Blot: NDUFA4L2 Antibody [NBP2-93831] - Analysis of extracts of various cell lines, using NDUFA4L2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.](http://images.novusbio.com/fullsize/NDUFA4L2-Antibody-Western-Blot-NBP2-93831-img0003.jpg "Western Blot: NDUFA4L2 Antibody [NBP2-93831] - Analysis of extracts of various cell lines, using NDUFA4L2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.")
![Immunocytochemistry/Immunofluorescence: NDUFA4L2 Antibody [NBP2-93831] - Immunofluorescence analysis of C6 cells using NDUFA4L2 antibody (NBP2-93831) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NDUFA4L2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93831-img0006.jpg)
![Immunohistochemistry-Paraffin: NDUFA4L2 Antibody [NBP2-93831] - Mouse kidney using NDUFA4L2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NDUFA4L2-Antibody-Immunohistochemistry-Paraffin-NBP2-93831-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NDUFA4L2 Antibody [NBP2-93831] - Analysis of L929 cells using NDUFA4L2 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NDUFA4L2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93831-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NDUFA4L2 Antibody [NBP2-93831] - Analysis of U-2 OS cells using NDUFA4L2 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NDUFA4L2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93831-img0002.jpg)
![Immunohistochemistry-Paraffin: NDUFA4L2 Antibody [NBP2-93831] - Human colon using NDUFA4L2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NDUFA4L2-Antibody-Immunohistochemistry-Paraffin-NBP2-93831-img0004.jpg)
![Western Blot: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Western blot analysis of extracts of various cell lines, using Ndufs4 Rabbit mAb (NBP3-16679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-6T10F4-Western-Blot-NBP3-16679-img0006.jpg "Western Blot: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Western blot analysis of extracts of various cell lines, using Ndufs4 Rabbit mAb (NBP3-16679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Immunofluorescence analysis of NIH-3T3 cells using Ndufs4 Rabbit mAb (NBP3-16679) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-6T10F4-Immunocytochemistry-Immunofluorescence-NBP3-16679-img0005.jpg)
![Western Blot: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Western blot analysis of extracts of HepG2 cells, using Ndufs4 Rabbit mAb (NBP3-16679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-6T10F4-Western-Blot-NBP3-16679-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Immunofluorescence analysis of C6 cells using Ndufs4 Rabbit mAb (NBP3-16679) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-6T10F4-Immunocytochemistry-Immunofluorescence-NBP3-16679-img0004.jpg)
![Immunohistochemistry-Paraffin: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Analysis of NDUFS4 in paraffin-embedded human colon carcinoma tissue using NDUFS4 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16679_rabbit-ndufs4-mab-6t10f4-immunohistochemistry-paraffin-272202491912..jpg)
![Immunohistochemistry-Paraffin: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Analysis of NDUFS4 in paraffin-embedded human liver tissue using NDUFS4 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16679_rabbit-ndufs4-mab-6t10f4-immunohistochemistry-paraffin-27220249233..jpg)