| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40773SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92344-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81592-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83349-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47365-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90815-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-57587SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34413-0.1mg | |||
![Western Blot: NF-M Antibody (3H11) [NB300-134] - Neuronal tissue lysates using mouse mAb to NF-M, dilution 1:10,000 in green: [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at ~145 kDa corresponds to rodent NF-M while that at about 160 kDa corresponds to the significantly larger bovine NF-M protein.](http://images.novusbio.com/fullsize/NF-M-Antibody-3H11-Western-Blot-NB300-134-img0004.jpg "Western Blot: NF-M Antibody (3H11) [NB300-134] - Neuronal tissue lysates using mouse mAb to NF-M, dilution 1:10,000 in green: [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at ~145 kDa corresponds to rodent NF-M while that at about 160 kDa corresponds to the significantly larger bovine NF-M protein.")
![Immunohistochemistry Free-Floating: NF-M Antibody (3H11) [NB300-134] - Adult rat frontal cortex section stained with mouse mAb to neurofilament NF-M, dilution 1:5,000 in green, and costained with chicken pAb to neurofilament NF-H, dilution 1:5,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NB300-134 antibody labels neuron cell bodies and dendrites of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the NF-H antibody stains the network of neuronal axons only.](http://images.novusbio.com/fullsize/NF-M-Antibody-3H11-Immunohistochemistry-NB300-134-img0003.jpg)
![Western Blot: 160kDa Neurofilament Medium Antibody (3H11) [NB300-134] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including NB300-134 (lane 3).](http://images.novusbio.com/fullsize/160kDa-Neurofilament-Medium-Antibody-3H11-Western-Blot-NB300-134-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DISC1 Antibody [NB110-40773] - DISC1 antibody was tested in PC-12 cells at 1:100 with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).](http://images.novusbio.com/fullsize/DISC1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40773-img0006.jpg "Immunocytochemistry/Immunofluorescence: DISC1 Antibody [NB110-40773] - DISC1 antibody was tested in PC-12 cells at 1:100 with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).")
![Immunohistochemistry: DISC1 Antibody [NB110-40773] - Staining of DISC1 in human brain neurons and neuropil at 2.5ug/ml. 40X.](http://images.novusbio.com/fullsize/DISC1-Antibody-Immunohistochemistry-NB110-40773-img0005.jpg)
![Western Blot: RPB9 Antibody [NBP1-92344] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/RPB9-Antibody-Western-Blot-NBP1-92344-img0010.jpg "Western Blot: RPB9 Antibody [NBP1-92344] - Analysis in human cell line HEK 293.")
![Immunocytochemistry/Immunofluorescence: RPB9 Antibody [NBP1-92344] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/RPB9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92344-img0009.jpg)
![Western Blot: RPB9 Antibody [NBP1-92344] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/RPB9-Antibody-Western-Blot-NBP1-92344-img0011.jpg)
![Western Blot: HIP1 Antibody [NBP1-81592] - Analysis in human cell lines A-549 and Caco-2 using Anti-HIP1 antibody. Corresponding HIP1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/HIP1-Antibody-Western-Blot-NBP1-81592-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: HIP1 Antibody [NBP1-81592] - Analysis in human cell lines A-549 and Caco-2 using Anti-HIP1 antibody. Corresponding HIP1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIP1 Antibody [NBP1-81592] - Staining of human cell line U-251 MG shows localization to vesicles.](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81592-img0018.jpg)
![Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human testis using Anti-HIP1 antibody NBP1-81592.](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81592-img0023.jpg)
![Western Blot: HIP1 Antibody [NBP1-81592] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/HIP1-Antibody-Western-Blot-NBP1-81592-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Analysis in human testis and liver tissues using Anti-HIP1 antibody. Corresponding HIP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81592-img0014.jpg)
![Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81592-img0016.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining in human testis and liver tissues using anti-HIP1 antibody. Corresponding HIP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81592-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human colon, kidney, liver and testis using Anti-HIP1 antibody NBP1-81592 (A) shows similar protein distribution across tissues to independent antibody NBP1-81593 (B).](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81592-img0019.jpg)
![Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human colon.](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81592-img0020.jpg)
![Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human kidney.](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81592-img0021.jpg)
![Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human liver using Anti-HIP1 antibody NBP1-81592.](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81592-img0022.jpg)
![Western Blot: LRPPRC Antibody [NBP1-83349] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LRPPRC antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Western-Blot-NBP1-83349-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LRPPRC Antibody [NBP1-83349] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LRPPRC antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LRPPRC Antibody [NBP1-83349] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83349-img0007.jpg)
![Western Blot: MT-ND4 Antibody [NBP2-47365] - Analysis in human cell line HeLa.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Western-Blot-NBP2-47365-img0003.jpg "Western Blot: MT-ND4 Antibody [NBP2-47365] - Analysis in human cell line HeLa.")
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human Stomach shows strong granular cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunohistochemistry-Paraffin-NBP2-47365-img0011.jpg)
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunohistochemistry-Paraffin-NBP2-47365-img0004.jpg)
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human Lymph node shows very weak granular cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunohistochemistry-Paraffin-NBP2-47365-img0009.jpg)
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human Salivary gland shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunohistochemistry-Paraffin-NBP2-47365-img0010.jpg)
![Western Blot: ATPB Antibody [NBP1-90815] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ATPB-Antibody-Western-Blot-NBP1-90815-img0015.jpg "Western Blot: ATPB Antibody [NBP1-90815] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: ATPB Antibody [NBP1-90815] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90815-img0007.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0020.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATPB Antibody [NBP1-90815] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATP5B antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/ATPB-Antibody-Western-Blot-NBP1-90815-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human kidney, liver, prostate and small intestine using Anti-ATP5B antibody NBP1-90815 (A) shows similar protein distribution across tissues to independent antibody NBP1-90816 (B).](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0016.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0018.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0019.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0021.jpg)
![Western Blot: SELENBP1 Antibody [NBP1-54805] - Antibody Titration: 1 ug/ml Human heart.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Western-Blot-NBP1-54805-img0006.jpg "Western Blot: SELENBP1 Antibody [NBP1-54805] - Antibody Titration: 1 ug/ml Human heart.")
![Immunohistochemistry: SELENBP1 Antibody [NBP1-54805] - Human Adult heart Observed Staining: Cytoplasmic,Membrane (weak) Primary Antibody Concentration: 1 : 600 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 2.0 sec.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-NBP1-54805-img0005.jpg)
![Western Blot: SELENBP1 Antibody [NBP1-54805] - Titration: 0.2-1 ug/ml, Positive Control: SH-SYSY cell lysate.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Western-Blot-NBP1-54805-img0003.jpg)
![Western Blot: SELENBP1 Antibody [NBP1-54805] - Antibody Titration: 1 ug/ml Human liver.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Western-Blot-NBP1-54805-img0007.jpg)
![Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-54805] - SELENBP1 in colon mucosa (Mouse), dilution 1:500, 10x, 20x and 40x. Image from verfied customer review.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-54805-img0004.jpg)
![Western Blot: CD45 Antibody (BL-178-12C7) [NBP3-14668] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 1 second. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/CD45-Antibody-BL-178-12C7-Western-Blot-NBP3-14668-img0013.jpg "Western Blot: CD45 Antibody (BL-178-12C7) [NBP3-14668] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 1 second. Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunocytochemistry/Immunofluorescence: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody [BL-178-12C7](A700-012). Secondary: HRP-conjugated goat anti-rabbitIgG.Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0006.jpg)
![Immunohistochemistry: CD45 Antibody [NBP3-14668] - Sample: FFPE section of human coloncarcinoma. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™.Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0007.jpg)
![Flow Cytometry: CD45 Antibody [NBP3-14668] - Sample: Jurkat cells were fixed in 4% formaldehyde andpermeabilized with 90% methanol. Antibody:1E6 cells were incubated with 1 ul of rabbit anti-CD45recombinant monoclonal antibody (red shaded) or isotype control(unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD45-Antibody-Flow-Cytometry-NBP3-14668-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody. Secondary: HRP-conjugated goat antirabbit IgG.Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0005.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody. Secondary: HRP-conjugated goat antirabbit IgG .Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0003.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody. Secondary: HRP-conjugated goat antirabbit IgG.Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0004.jpg)
![Immunoprecipitation: CD45 Antibody [NBP3-14668] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from Jurkat cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-CD45recombinant monoclonal antibody used for IP at 20 ul/mg lysate. CD45 wasalso immunoprecipitated by a previous lot of this antibodyand rabbit anti-CD45 antibody. For blotting immunoprecipitated CD45,was used at 1:1000. Chemiluminescence withan exposure time of 250 milliseconds.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunoprecipitation-NBP3-14668-img0002.jpg)
![Western Blot: Tmp21/p23 Antibody [NB110-57587] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TMP21/TMED10 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human TMP21/TMED10 Polyclonal Antibody (Catalog # NB110-57587) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TMP21/TMED10 at approximately 25 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reduc](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Knockdown-Validated-NB110-57587-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Tmp21/p23 Antibody [NB110-57587] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TMP21/TMED10 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human TMP21/TMED10 Polyclonal Antibody (Catalog # NB110-57587) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TMP21/TMED10 at approximately 25 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reduc")
![<b>Genetic Strategies Validation. </b>Western Blot: TMP21 Antibody [NB110-57587] - Detection of TMP21 in human liver lysate using NB110-57587.](http://images.novusbio.com/fullsize/TMP21-Antibody-Western-Blot-NB110-57587-img0003.jpg)
![Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NB110-57587] - Tmp21/p23 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human TMP21/p23 polyclonal Antibody (Catalog # NB110-57587) at 1 ug/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in hepatocytes.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NB110-57587-img0004.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser276] Antibody-Western Blot-NBP1-77807-img0010.jpg "Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method.")
![Immunohistochemistry: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - Diluted to1:500 to detect p65 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunohistochemistry-NBP1-77807-img0007.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS529 shows phospho p65 staining in carcinoma cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0005.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS276 shows phospho p65 staining in carcinoma cells. Western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS276 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes and does not show significant levels of phosphorylated p65. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0006.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0008.jpg)
![Western Blot: GFAP Antibody (SPM507) [NBP2-34365] - Western Blot Analysis of human Brain tissue lysate using GFAP Antibody (SPM507).](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM507-Western-Blot-NBP2-34365-img0004.jpg "Western Blot: GFAP Antibody (SPM507) [NBP2-34365] - Western Blot Analysis of human Brain tissue lysate using GFAP Antibody (SPM507).")
![Immunohistochemistry-Paraffin: GFAP Antibody (SPM507) [NBP2-34365] - Formalin-paraffin human brain stained with GFAP Ab (SPM507). Note cytoplasmic staining.](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM507-Immunohistochemistry-Paraffin-NBP2-34365-img0001.jpg)