![Western Blot: Syntaxin Antibody (7D9V8) [NBP3-15934] - Western blot analysis of extracts of various cell lines, using Syntaxin antibody (NBP3-15934) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Syntaxin-Antibody-7D9V8-Western-Blot-NBP3-15934-img0006.jpg "Western Blot: Syntaxin Antibody (7D9V8) [NBP3-15934] - Western blot analysis of extracts of various cell lines, using Syntaxin antibody (NBP3-15934) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, IHC |
| Clone | 7D9V8 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Syntaxin (P61266). MKDRTQELRSAKDSDDEEEVVHVDRDHFMDEFFEQVEEIRGCIEKLSEDVEQVKKQHSAILAAPNPDEKTKQELEDLTADIKKTANKVRSKLKAIEQSIE |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:20 |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol, 0.05% BSA |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Syntaxin Antibody (7D9V8) [NBP3-15934] - Western blot analysis of extracts of Rat spinal cord, using Syntaxin antibody (NBP3-15934) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Syntaxin-Antibody-7D9V8-Western-Blot-NBP3-15934-img0007.jpg "Western Blot: Syntaxin Antibody (7D9V8) [NBP3-15934] - Western blot analysis of extracts of Rat spinal cord, using Syntaxin antibody (NBP3-15934) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry: Syntaxin Antibody (7D9V8) [NBP3-15934] - Immunohistochemistry analysis of Syntaxin in paraffin-embedded mouse brain tissue using Syntaxin Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15934_rabbit-syntaxin-mab-7d9v8-72202512435342.jpg "Immunohistochemistry: Syntaxin Antibody (7D9V8) [NBP3-15934] - Immunohistochemistry analysis of Syntaxin in paraffin-embedded mouse brain tissue using Syntaxin Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: Syntaxin Antibody (7D9V8) [NBP3-15934] - Immunofluorescence analysis of paraffin-embedded mouse brain using Syntaxin Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15934_rabbit-syntaxin-mab-7d9v8-7220251243536.jpg "Immunocytochemistry/ Immunofluorescence: Syntaxin Antibody (7D9V8) [NBP3-15934] - Immunofluorescence analysis of paraffin-embedded mouse brain using Syntaxin Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry: Syntaxin Antibody (7D9V8) [NBP3-15934] - Immunohistochemistry analysis of Syntaxin in paraffin-embedded human brain tissue using Syntaxin Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15934_rabbit-syntaxin-mab-7d9v8-7220251245337.jpg "Immunohistochemistry: Syntaxin Antibody (7D9V8) [NBP3-15934] - Immunohistochemistry analysis of Syntaxin in paraffin-embedded human brain tissue using Syntaxin Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Syntaxin Antibody (7D9V8) [NBP3-15934] - Immunohistochemistry analysis of Syntaxin in paraffin-embedded mouse testis tissue using Syntaxin Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15934_rabbit-syntaxin-mab-7d9v8-72202512443332.jpg "Immunohistochemistry: Syntaxin Antibody (7D9V8) [NBP3-15934] - Immunohistochemistry analysis of Syntaxin in paraffin-embedded mouse testis tissue using Syntaxin Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Syntaxin Antibody (7D9V8) [NBP3-15934] - Immunohistochemistry analysis of Syntaxin in paraffin-embedded rat brain tissue using Syntaxin Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15934_rabbit-syntaxin-mab-7d9v8-72202512443320.jpg "Immunohistochemistry: Syntaxin Antibody (7D9V8) [NBP3-15934] - Immunohistochemistry analysis of Syntaxin in paraffin-embedded rat brain tissue using Syntaxin Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Syntaxin Antibody (7D9V8) [NBP3-15934] - Immunohistochemistry analysis of Syntaxin in paraffin-embedded human colon tissue using Syntaxin Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15934_rabbit-syntaxin-mab-7d9v8-72202512443368.jpg "Immunohistochemistry: Syntaxin Antibody (7D9V8) [NBP3-15934] - Immunohistochemistry analysis of Syntaxin in paraffin-embedded human colon tissue using Syntaxin Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Syntaxin Antibody (7D9V8) [NBP3-15934] - Immunohistochemistry analysis of Syntaxin in paraffin-embedded human pancreas tissue using Syntaxin Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15934_rabbit-syntaxin-mab-7d9v8-72202512443390.jpg "Immunohistochemistry: Syntaxin Antibody (7D9V8) [NBP3-15934] - Immunohistochemistry analysis of Syntaxin in paraffin-embedded human pancreas tissue using Syntaxin Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: Syntaxin Antibody (7D9V8) [NBP3-15934] - Immunofluorescence analysis of paraffin-embedded rat brain using Syntaxin Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15934_rabbit-syntaxin-mab-7d9v8-72202512432094.jpg "Immunocytochemistry/ Immunofluorescence: Syntaxin Antibody (7D9V8) [NBP3-15934] - Immunofluorescence analysis of paraffin-embedded rat brain using Syntaxin Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")