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![Western Blot: Kif4A Antibody [NB100-57512] - Detection of Human KIF4A on HeLa whole cell lysate using NB100-57512. KIF4A was also immunoprecipitated by rabbit anti-KIF4A antibody NB100-57511.](http://images.novusbio.com/fullsize/Kif4A-Antibody-Western-Blot-NB100-57512-img0001.jpg "Western Blot: Kif4A Antibody [NB100-57512] - Detection of Human KIF4A on HeLa whole cell lysate using NB100-57512. KIF4A was also immunoprecipitated by rabbit anti-KIF4A antibody NB100-57511.")
![Western Blot: CNAP1 Antibody [NB100-1570] - Detection of Human CAP-D2 on HeLa whole cell lysate using NB100-1570.](http://images.novusbio.com/fullsize/CNAP1-Antibody-Western-Blot-NB100-1570-img0002.jpg "Western Blot: CNAP1 Antibody [NB100-1570] - Detection of Human CAP-D2 on HeLa whole cell lysate using NB100-1570.")
![Western Blot: CNAP1 Antibody (5E0Q9) [NBP3-33550] - Western blot analysis of lysates from HeLa cells, using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33550_rabbit-cnap1-mab-5e0q9-10122024140450.jpg "Western Blot: CNAP1 Antibody (5E0Q9) [NBP3-33550] - Western blot analysis of lysates from HeLa cells, using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: CNAP1 Antibody (5E0Q9) [NBP3-33550] - Western blot analysis of various lysates, using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33550_rabbit-cnap1-mab-5e0q9-10122024140438.jpg)
![Western Blot: NUP98 Antibody (6M6V6) [NBP3-33379] - Western blot analysis of lysates from HeLa cells, using NUP98 Rabbit mAb at 1:6000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33379_rabbit-nup98-mab-6m6v6-912202415175242.jpg "Western Blot: NUP98 Antibody (6M6V6) [NBP3-33379] - Western blot analysis of lysates from HeLa cells, using NUP98 Rabbit mAb at 1:6000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: NUP98 Antibody (6M6V6) [NBP3-33379] - Western blot analysis of various lysates using NUP98 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33379_rabbit-nup98-mab-6m6v6-5220251242575.jpg)
![Western Blot: Bub1 Antibody (6U8G10) [NBP3-33408] - Western blot analysis of lysates from HEL cells, using [KD Validated] Bub1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33408_rabbit-bub1-mab-6u8g10-1012202411535835.jpg "Western Blot: Bub1 Antibody (6U8G10) [NBP3-33408] - Western blot analysis of lysates from HEL cells, using [KD Validated] Bub1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Bub1 Antibody (6U8G10) [NBP3-33408] - Western blot analysis of lysates from wild type(WT) and Bub1 knockdown (KD) 293T cells, using [KD Validated] Bub1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33408_rabbit-bub1-mab-6u8g10-52202511374121.jpg)
![Western Blot: Cyclin B1 Antibody (1Q3E9) [NBP3-15626] - Western blot analysis of lysates from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells using [KO Validated] Cyclin B1 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-15626_rabbit-cyclin-b1-mab-1q3e9-82202520986.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cyclin B1 Antibody (1Q3E9) [NBP3-15626] - Western blot analysis of lysates from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells using [KO Validated] Cyclin B1 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclin B1 Antibody (1Q3E9) [Cyclin B1] - Western blot analysis of lysates from Jurkat cells using [KO Validated] Cyclin B1 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-15626_rabbit-cyclin-b1-mab-1q3e9-52202517352744.jpg)
![Western Blot: Pin1 Antibody [NB100-53805] - (0.2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Pin1-Antibody-Western-Blot-NB100-53805-img0001.jpg "Western Blot: Pin1 Antibody [NB100-53805] - (0.2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Kif4A Antibody [NB100-1053] - Staining (green) of 4% formaldahyde-fixed dividing HeLa with endogenously expressed Histone H2B-GFP fusion as DNA marker (red). Data kindly provided by Francisco Iborra, MRC Mol. Haematol. Unit, WIMM, JR Hospital Oxford.](http://images.novusbio.com/fullsize/Kif4A-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1053-img0001.jpg "Immunocytochemistry/Immunofluorescence: Kif4A Antibody [NB100-1053] - Staining (green) of 4% formaldahyde-fixed dividing HeLa with endogenously expressed Histone H2B-GFP fusion as DNA marker (red). Data kindly provided by Francisco Iborra, MRC Mol. Haematol. Unit, WIMM, JR Hospital Oxford.")
![Western Blot: ZWILCH Antibody [H00055055-D01P] - Analysis of ZWILCH expression in mouse spleen.](http://images.novusbio.com/fullsize/ZWILCH-Antibody-Western-Blot-H00055055-D01P-img0001.jpg "Western Blot: ZWILCH Antibody [H00055055-D01P] - Analysis of ZWILCH expression in mouse spleen.")
![Western Blot: ZWILCH Antibody [H00055055-D01P] - Analysis of ZWILCH expression in transfected 293T cell line by ZWILCH polyclonal antibody.Lane 1: ZWILCH transfected lysate(54.20 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ZWILCH-Antibody-Western-Blot-H00055055-D01P-img0002.jpg)
![Western Blot: CKS1 Antibody [H00001163-B01P] - Analysis of CKS1B expression in rat brain.](http://images.novusbio.com/fullsize/CKS1-Antibody-Western-Blot-H00001163-B01P-img0001.jpg "Western Blot: CKS1 Antibody [H00001163-B01P] - Analysis of CKS1B expression in rat brain.")
![Western Blot: CKS1 Antibody [H00001163-B01P] - Analysis of CKS1B expression in transfected 293T cell line by CKS1B polyclonal antibody. Lane 1: CKS1B transfected lysate(8.69 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CKS1-Antibody-Western-Blot-H00001163-B01P-img0002.jpg)
![Western Blot: NUP98 Antibody [NBP2-19612] - Sample (30 ug of whole cell lysate) A: JurKat 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NUP98-Antibody-Western-Blot-NBP2-19612-img0001.jpg "Western Blot: NUP98 Antibody [NBP2-19612] - Sample (30 ug of whole cell lysate) A: JurKat 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunoprecipitation: NUP98 Antibody [NBP2-19612] - HeLa whole cell extracts using 5 ug of NUP98 antibody [N3C1], Internal. Western blot analysis was performed using NUP98 antibody [N3C1], Internal. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/NUP98-Antibody-Immunoprecipitation-NBP2-19612-img0004.jpg)
![Western Blot: CHFR Antibody [NBP1-30986] - CHFR antibody detects CHFR protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5 % SDS-PAGE, and blotted with CHFR antibody diluted by 1:500](http://images.novusbio.com/fullsize/CHFR-Antibody-Western-Blot-NBP1-30986-img0004.jpg "Western Blot: CHFR Antibody [NBP1-30986] - CHFR antibody detects CHFR protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5 % SDS-PAGE, and blotted with CHFR antibody diluted by 1:500")
![Western Blot: CHFR Antibody [NBP1-30986] - Sample (30 ug of whole cell lysate) A: H12997 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CHFR-Antibody-Western-Blot-NBP1-30986-img0002.jpg)