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![Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PLK4 antibody [HL3371] (NBP3-48797) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48797_rabbit-plk4-mab-hl3371-western-blot-141120251841524.jpg "Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PLK4 antibody [HL3371] (NBP3-48797) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![PLK4 antibody [HL3371] detects PLK4 protein by immunohistochemical analysis.Sample: Paraffin-embedded human placenta.PLK4 stained by PLK4 antibody [HL3371] (NBP3-48797) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48797_rabbit-plk4-mab-hl3371-immunohistochemistry-paraffin-141120251834498.jpg)
![Western Blot:[NBP3-29063]-Detection of human and mouse NPM1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NPM1 antibody used for WB at 0. 04 µg/ml and 0. 1 µg/ml and used for IP at 10 µg/mg lysate. NPM1 was successfully immunoprecipitated by rabbit anti-NPM1 antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds and 3 seconds.](http://images.novusbio.com/fullsize/nbp3-29063_rabbit-npm1-pab-23120251204028.jpg "Western Blot:[NBP3-29063]-Detection of human and mouse NPM1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NPM1 antibody used for WB at 0. 04 µg/ml and 0. 1 µg/ml and used for IP at 10 µg/mg lysate. NPM1 was successfully immunoprecipitated by rabbit anti-NPM1 antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds and 3 seconds.")
![Immunocytochemistry/Immunofluorescence: Kif4A Antibody [NB100-1053] - Staining (green) of 4% formaldahyde-fixed dividing HeLa with endogenously expressed Histone H2B-GFP fusion as DNA marker (red). Data kindly provided by Francisco Iborra, MRC Mol. Haematol. Unit, WIMM, JR Hospital Oxford.](http://images.novusbio.com/fullsize/Kif4A-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1053-img0001.jpg "Immunocytochemistry/Immunofluorescence: Kif4A Antibody [NB100-1053] - Staining (green) of 4% formaldahyde-fixed dividing HeLa with endogenously expressed Histone H2B-GFP fusion as DNA marker (red). Data kindly provided by Francisco Iborra, MRC Mol. Haematol. Unit, WIMM, JR Hospital Oxford.")
![Western Blot: Pin1 Antibody [NB100-53805] - (0.2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Pin1-Antibody-Western-Blot-NB100-53805-img0001.jpg "Western Blot: Pin1 Antibody [NB100-53805] - (0.2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: CDC2/CDK1 [p Thr14] Antibody (6R1O7) [NBP3-16913] - Analysis of extracts of various cell lines, using CDC2/CDK1 Rabbit mAb (NBP3-16913) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37C for 20 hours or treated by Nocodazole (100 ng/mL) at 37C for 24 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CDC2-CDK1-p-Thr14-Antibody-6R1O7-Western-Blot-NBP3-16913-img0001.jpg "Western Blot: CDC2/CDK1 [p Thr14] Antibody (6R1O7) [NBP3-16913] - Analysis of extracts of various cell lines, using CDC2/CDK1 Rabbit mAb (NBP3-16913) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37C for 20 hours or treated by Nocodazole (100 ng/mL) at 37C for 24 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Aurora B Antibody (2B1) [NBP1-74027] - The recombinant proteins (50ng) were resolved by SDSPAGE,transferred to PVDF membrane and probed with antihumanAurora kinase B antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibodyconjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human Aurora kinase ALane 2.: Recombinant Human Aurora kinase B](http://images.novusbio.com/fullsize/Aurora-B-Antibody-2B1-Western-Blot-NBP1-74027-img0005.jpg "Western Blot: Aurora B Antibody (2B1) [NBP1-74027] - The recombinant proteins (50ng) were resolved by SDSPAGE,transferred to PVDF membrane and probed with antihumanAurora kinase B antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibodyconjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human Aurora kinase ALane 2.: Recombinant Human Aurora kinase B")
![Flow Cytometry: Aurora B Antibody (2B1) [NBP1-74027] - Analysis of Aurora B in LNCap cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/Aurora-B-Antibody-2B1-Flow-Cytometry-NBP1-74027-img0006.jpg)
![Western Blot: NEK2 Antibody (5O0A1) [NBP3-16641] - Western blot analysis of extracts of various cell lines, using NEK2 Rabbit mAb (NBP3-16641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/NEK2-Antibody-5O0A1-Western-Blot-NBP3-16641-img0001.jpg "Western Blot: NEK2 Antibody (5O0A1) [NBP3-16641] - Western blot analysis of extracts of various cell lines, using NEK2 Rabbit mAb (NBP3-16641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Pin1 Antibody (3G8) [NBP1-22956] - Cell lysates of 293T, HeLa and Jurkat (each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Pin1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/Pin1-Antibody-3G8-Western-Blot-NBP1-22956-img0002.jpg "Western Blot: Pin1 Antibody (3G8) [NBP1-22956] - Cell lysates of 293T, HeLa and Jurkat (each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Pin1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Flow Cytometry: Pin1 Antibody (3G8) [NBP1-22956] - Analysis of Pin1 in Jurkat cell line, staining at2-5ug for 1x106cells (red line). The secondary antibody usedgoat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/Pin1-Antibody-3G8-Flow-Cytometry-NBP1-22956-img0003.jpg)
![Western Blot: Bub1 Antibody (1N8M1) [NBP3-33398] - Western blot analysis of various lysates, using Bub1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33398_rabbit-bub1-mab-1n8m1-1012202411343381.jpg "Western Blot: Bub1 Antibody (1N8M1) [NBP3-33398] - Western blot analysis of various lysates, using Bub1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![Western Blot: PLK4 Antibody [NBP3-37985] - Western blot analysis of various lysates using PLK4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-37985_rabbit-plk4-pab-101220241134335.jpg "Western Blot: PLK4 Antibody [NBP3-37985] - Western blot analysis of various lysates using PLK4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.")
![Western Blot: Bub1 Antibody [NBP3-03432] - Analysis of extracts from normal (control) and BUB1 knockout (KO) HeLa cells, using Bub1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Bub1-Antibody-Western-Blot-NBP3-03432-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Bub1 Antibody [NBP3-03432] - Analysis of extracts from normal (control) and BUB1 knockout (KO) HeLa cells, using Bub1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: BubR1 Antibody - N-terminal [NBP3-30806] - Immunoprecipitation/Western blot analysis using Bub1b-NT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flowcytometry.](http://images.novusbio.com/fullsize/nbp3-30806_sheep-bubr1-pab-n-terminal-811202420594239.jpg "Western Blot: BubR1 Antibody - N-terminal [NBP3-30806] - Immunoprecipitation/Western blot analysis using Bub1b-NT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flowcytometry.")