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![Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PLK4 antibody [HL3371] (NBP3-48797) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48797_rabbit-plk4-mab-hl3371-western-blot-141120251841524.jpg "Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PLK4 antibody [HL3371] (NBP3-48797) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![PLK4 antibody [HL3371] detects PLK4 protein by immunohistochemical analysis.Sample: Paraffin-embedded human placenta.PLK4 stained by PLK4 antibody [HL3371] (NBP3-48797) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48797_rabbit-plk4-mab-hl3371-immunohistochemistry-paraffin-141120251834498.jpg)
![Western Blot: Pin1 Antibody (3G8) [NBP1-22956] - Cell lysates of 293T, HeLa and Jurkat (each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Pin1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/Pin1-Antibody-3G8-Western-Blot-NBP1-22956-img0002.jpg "Western Blot: Pin1 Antibody (3G8) [NBP1-22956] - Cell lysates of 293T, HeLa and Jurkat (each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Pin1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Flow Cytometry: Pin1 Antibody (3G8) [NBP1-22956] - Analysis of Pin1 in Jurkat cell line, staining at2-5ug for 1x106cells (red line). The secondary antibody usedgoat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/Pin1-Antibody-3G8-Flow-Cytometry-NBP1-22956-img0003.jpg)
![Western Blot: Aurora B Antibody (2B1) [NBP1-74027] - The recombinant proteins (50ng) were resolved by SDSPAGE,transferred to PVDF membrane and probed with antihumanAurora kinase B antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibodyconjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human Aurora kinase ALane 2.: Recombinant Human Aurora kinase B](http://images.novusbio.com/fullsize/Aurora-B-Antibody-2B1-Western-Blot-NBP1-74027-img0005.jpg "Western Blot: Aurora B Antibody (2B1) [NBP1-74027] - The recombinant proteins (50ng) were resolved by SDSPAGE,transferred to PVDF membrane and probed with antihumanAurora kinase B antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibodyconjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human Aurora kinase ALane 2.: Recombinant Human Aurora kinase B")
![Flow Cytometry: Aurora B Antibody (2B1) [NBP1-74027] - Analysis of Aurora B in LNCap cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/Aurora-B-Antibody-2B1-Flow-Cytometry-NBP1-74027-img0006.jpg)
![Western Blot: BubR1 Antibody - C-terminus [NBP3-30805] - Immunoprecipitation/Western blot analysis using Bub1b-CT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flowcytometry.](http://images.novusbio.com/fullsize/nbp3-30805_sheep-bubr1-pab-c-terminus-811202420592120.jpg "Western Blot: BubR1 Antibody - C-terminus [NBP3-30805] - Immunoprecipitation/Western blot analysis using Bub1b-CT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flowcytometry.")
![Western Blot: BubR1 Antibody [NBP3-18818] - Immunoprecipitation/ Western blot analysis on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flow cytometry](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NBP3-18818-img0001.jpg "Western Blot: BubR1 Antibody [NBP3-18818] - Immunoprecipitation/ Western blot analysis on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flow cytometry")
![Western Blot: BubR1 Antibody - N-terminal [NBP3-30806] - Immunoprecipitation/Western blot analysis using Bub1b-NT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flowcytometry.](http://images.novusbio.com/fullsize/nbp3-30806_sheep-bubr1-pab-n-terminal-811202420594239.jpg "Western Blot: BubR1 Antibody - N-terminal [NBP3-30806] - Immunoprecipitation/Western blot analysis using Bub1b-NT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flowcytometry.")
![Western Blot:[NBP3-29063]-Detection of human and mouse NPM1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NPM1 antibody used for WB at 0. 04 µg/ml and 0. 1 µg/ml and used for IP at 10 µg/mg lysate. NPM1 was successfully immunoprecipitated by rabbit anti-NPM1 antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds and 3 seconds.](http://images.novusbio.com/fullsize/nbp3-29063_rabbit-npm1-pab-23120251204028.jpg "Western Blot:[NBP3-29063]-Detection of human and mouse NPM1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NPM1 antibody used for WB at 0. 04 µg/ml and 0. 1 µg/ml and used for IP at 10 µg/mg lysate. NPM1 was successfully immunoprecipitated by rabbit anti-NPM1 antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds and 3 seconds.")
![Western Blot: H2AX Protein [NBP1-42464] - The anti-H2AX antibody NB100-638 was used to detect this protein. 200ng of protein was loaded onto the gel and NB100-638 was used to detect the protein.](http://images.novusbio.com/fullsize/H2AX-Protein-Western-Blot-NBP1-42464-img0002.jpg "Western Blot: H2AX Protein [NBP1-42464] - The anti-H2AX antibody NB100-638 was used to detect this protein. 200ng of protein was loaded onto the gel and NB100-638 was used to detect the protein.")
![SDS-Page: H2AX Protein [NBP1-42464] - This H2AX protein was tested on a 4-20% SDS-PAGE gel with 5ug of protein loaded on the gel. The purity of the protein is >95%.](http://images.novusbio.com/fullsize/H2AX-Protein-Immunoblotting-NBP1-42464-img0001.jpg)
![Western Blot: NEK2 Antibody (5O0A1) [NBP3-16641] - Western blot analysis of extracts of various cell lines, using NEK2 Rabbit mAb (NBP3-16641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/NEK2-Antibody-5O0A1-Western-Blot-NBP3-16641-img0001.jpg "Western Blot: NEK2 Antibody (5O0A1) [NBP3-16641] - Western blot analysis of extracts of various cell lines, using NEK2 Rabbit mAb (NBP3-16641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: CDC2/CDK1 [p Thr14] Antibody (6R1O7) [NBP3-16913] - Analysis of extracts of various cell lines, using CDC2/CDK1 Rabbit mAb (NBP3-16913) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37C for 20 hours or treated by Nocodazole (100 ng/mL) at 37C for 24 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CDC2-CDK1-p-Thr14-Antibody-6R1O7-Western-Blot-NBP3-16913-img0001.jpg "Western Blot: CDC2/CDK1 [p Thr14] Antibody (6R1O7) [NBP3-16913] - Analysis of extracts of various cell lines, using CDC2/CDK1 Rabbit mAb (NBP3-16913) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37C for 20 hours or treated by Nocodazole (100 ng/mL) at 37C for 24 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: PRC1 Antibody [NBP2-99648] - Immunochemical staining of human PRC1 in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PRC1-Antibody-Immunohistochemistry-Paraffin-NBP2-99648-img0002.jpg "Immunohistochemistry-Paraffin: PRC1 Antibody [NBP2-99648] - Immunochemical staining of human PRC1 in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.")
![Immunocytochemistry/Immunofluorescence: PRC1 Antibody [NBP2-99648] - Immunofluorescence staining of PRC1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99648 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm and cell membrane.](http://images.novusbio.com/fullsize/nbp2-99648_rabbit-polyclonal-prc1-antibody-23520251628112.png)
![Western Blot: Bub1 Antibody (1N8M1) [NBP3-33398] - Western blot analysis of various lysates, using Bub1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33398_rabbit-bub1-mab-1n8m1-1012202411343381.jpg "Western Blot: Bub1 Antibody (1N8M1) [NBP3-33398] - Western blot analysis of various lysates, using Bub1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")