| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-77452SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-111SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94822-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94900-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49263-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98994-50ul | |||
![Western Blot: TSG101 Antibody [NBP2-77452] - Total protein from human HeLa, HepG2, mouse 3T3 and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-TSG101 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP2-77452-img0002.jpg "Western Blot: TSG101 Antibody [NBP2-77452] - Total protein from human HeLa, HepG2, mouse 3T3 and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-TSG101 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-77452] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TSG101 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-77452-img0004.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-77452] - Analysis of a FFPE tissue section of the human small intestine using 1:200 dilution of TSG101 antibody (NBP2-77452). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated staining mainly in the cytoplasm and membrane of glandular cells.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-77452-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-77452] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TSG101 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-77452-img0003.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-111-img0007.jpg "Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)")
![Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody [NB400-111] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Immunocytochemistry-Immunofluorescence-NB400-111-img0001.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-111] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY LIPG cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-111-img0006.jpg)
![Western Blot: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of TSG101 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysateLane 2: Hela cell lysate](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Western-Blot-NBP2-67884-img0010.jpg "Western Blot: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of TSG101 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysateLane 2: Hela cell lysate")
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody (JJ0900) [NBP2-67884] - Staining TSG101 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunocytochemistry-Immunofluorescence-NBP2-67884-img0009.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of paraffin-embedded mouse kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunohistochemistry-Paraffin-NBP2-67884-img0007.jpg)
![Flow Cytometry: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of K562 cells with TSG101 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Flow-Cytometry-NBP2-67884-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody (JJ0900) [NBP2-67884] - Staining TSG101 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunocytochemistry-Immunofluorescence-NBP2-67884-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody (JJ0900) [NBP2-67884] - Staining TSG101 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunocytochemistry-Immunofluorescence-NBP2-67884-img0003.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of paraffin-embedded human colon cancer tissue using anti-TSG101 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunohistochemistry-Paraffin-NBP2-67884-img0004.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of paraffin-embedded human kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunohistochemistry-Paraffin-NBP2-67884-img0005.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of paraffin-embedded mouse colon tissue using anti-TSG101 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunohistochemistry-Paraffin-NBP2-67884-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of TSG101 with anti-TSG101 antibody [JJ0900] at 1:500 dilution.Lane 1: Wild-type Hela whole cell lysate (10 ug). Lane 2/3: TSG101 knockdown Hela whole cell lysate (10 ug). NBP2-67884 was shown to specifically react with TSG101 in wild-type Hela cells. Weakened bands were observed when TSG101 knockdown samples were tested. Wild-type and TSG101 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Knockdown-Validated-NBP2-67884-img0012.jpg)
![Simple Western: TSG101 Antibody (JJ0900) [NBP2-67884] - Simple Western Lane view shows K562 whole cell lysate (WCL). A specific band was detected for TSG101 antibody (NBP2-67884) at approximately 53 kDa (as indicated) using 1:50 ug/mL of TSG101 antibody. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Simple-Western-NBP2-67884-img0013.jpg)
![Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425] - Staining of paraffin embedded Human Prostate. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/VPS41-Antibody-Immunohistochemistry-Paraffin-NB100-2425-img0005.jpg "Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425] - Staining of paraffin embedded Human Prostate. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425] - Staining of paraffin embedded Human Kidney. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH9.](http://images.novusbio.com/fullsize/VPS41-Antibody-Immunohistochemistry-Paraffin-NB100-2425-img0003.jpg)
![Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425] - Staining of paraffin embedded Human Placenta. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/VPS41-Antibody-Immunohistochemistry-Paraffin-NB100-2425-img0004.jpg)
![Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (NBP3-13656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-RB-Western-Blot-NBP3-13656-img0001.jpg "Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (NBP3-13656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (NBP3-13656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13656_rabbit-tsg101-mab-4a10-rb-9102024202190.jpg)
![Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (NBP3-13656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13656_rabbit-tsg101-mab-4a10-rb-91020242001026.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13656_rabbit-tsg101-mab-4a10-rb-1610202419414543.jpg)
![Western Blot: TSG101 Antibody - Azide and BSA Free [TSG101] - Western blot analysis of lysates from K-562 cells using TSG101 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 120s.](http://images.novusbio.com/fullsize/nbp2-94822_rabbit-polyclonal-tsg101-antibody-52202516321425.jpg "Western Blot: TSG101 Antibody - Azide and BSA Free [TSG101] - Western blot analysis of lysates from K-562 cells using TSG101 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 120s.")
![<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody - Azide and BSA Free [TSG101] - Western blot analysis of lysates from wild type (WT) and TSG101 knockdown (KD) SH-SY5Y cells using TSG101 Rabbit pAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 120s.](http://images.novusbio.com/fullsize/nbp2-94822_rabbit-polyclonal-tsg101-antibody-52202516283277.jpg)
![Immunocytochemistry/ Immunofluorescence: TSG101 Antibody - Azide and BSA Free [TSG101] - Immunofluorescence analysis of PC-12 cells using TSG101 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94822_rabbit-polyclonal-tsg101-antibody-5220251638068.jpg)
![Immunocytochemistry/ Immunofluorescence: TSG101 Antibody - Azide and BSA Free [TSG101] - Immunofluorescence analysis of Jurkat cells using TSG101 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94822_rabbit-polyclonal-tsg101-antibody-5220251626465.jpg)
![Western Blot: TSG101 Antibody - Azide and BSA Free [NBP2-94900] - Western blot analysis of extracts from normal (control) and TSG101 knockdown (KD) SH-SY5Y(KD) cells, using TSG101 Rabbit pAb (A2216) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-94900_rabbit-polyclonal-tsg101-antibody-52202519294535.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody - Azide and BSA Free [NBP2-94900] - Western blot analysis of extracts from normal (control) and TSG101 knockdown (KD) SH-SY5Y(KD) cells, using TSG101 Rabbit pAb (A2216) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.")
![<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody - Azide and BSA Free [NBP2-94900] - Western blot analysis of K-562, using TSG101 Rabbit pAb (A2216) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-94900_rabbit-polyclonal-tsg101-antibody-52202519302972.jpg)
![Immunocytochemistry/ Immunofluorescence: TSG101 Antibody - Azide and BSA Free [NBP2-94900] - Immunofluorescence analysis of Jurkat cells using TSG101 Rabbit pAb (A2216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94900_rabbit-polyclonal-tsg101-antibody-52202519313476.jpg)
![Immunocytochemistry/ Immunofluorescence: TSG101 Antibody - Azide and BSA Free [NBP2-94900] - Immunofluorescence analysis of PC-12 cells using TSG101 Rabbit pAb (A2216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94900_rabbit-polyclonal-tsg101-antibody-522025193131.jpg)
![Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-49263] - Staining of human endometrium shows moderate granular cytoplasmic positivity in in glandular cells.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Immunohistochemistry-Paraffin-NBP2-49263-img0004.jpg "Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-49263] - Staining of human endometrium shows moderate granular cytoplasmic positivity in in glandular cells.")
![Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-49263] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Immunohistochemistry-Paraffin-NBP2-49263-img0002.jpg)
![Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-49263] - Staining of human urinary bladder shows moderate granular cytoplasmic positivity in in urothelial cells.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Immunohistochemistry-Paraffin-NBP2-49263-img0003.jpg)
![Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-49263] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Immunohistochemistry-Paraffin-NBP2-49263-img0005.jpg)
![Flow Cytometry: Integrin alpha 4/CD49d Antibody (9F10) [NBP1-43398] - Staining of normal human peripheral blood cells with 0.125 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.125 ug of Anti-Human CD49d Purified (filled histogram) followed by Anti-Mouse IgG FITC. Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/Integrin-alpha-4-CD49d-Antibody-9F10-Flow-Cytometry-NBP1-43398-img0001.jpg "Flow Cytometry: Integrin alpha 4/CD49d Antibody (9F10) [NBP1-43398] - Staining of normal human peripheral blood cells with 0.125 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.125 ug of Anti-Human CD49d Purified (filled histogram) followed by Anti-Mouse IgG FITC. Cells in the lymphocyte gate were used for analysis.")
![Immunofluorescence: Integrin alpha 4/CD49d Antibody (9F10) [NBP1-43398] - Analysis was performed using 70% confluent log phase MOLT-4 cells.](http://images.novusbio.com/fullsize/Integrin-alpha-4-CD49d-Antibody-9F10-Immunofluorescence-NBP1-43398-img0002.jpg)
![Western Blot: Integrin alpha 4/CD49d Antibody (8M8C2) [NBP3-16322] - Analysis of extracts of various cell lines, using Integrin alpha 4/CD49d (ITGA4/CD49d) Rabbit mAb (NBP3-16322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Integrin-alpha-4-CD49d-Antibody-8M8C2-Western-Blot-NBP3-16322-img0002.jpg "Western Blot: Integrin alpha 4/CD49d Antibody (8M8C2) [NBP3-16322] - Analysis of extracts of various cell lines, using Integrin alpha 4/CD49d (ITGA4/CD49d) Rabbit mAb (NBP3-16322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Integrin alpha 4/CD49d Antibody (8M8C2) [NBP3-16322] - Mouse testis using Integrin alpha 4/CD49d (ITGA4/CD49d) Rabbit mAb (NBP3-16322) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Integrin-alpha-4-CD49d-Antibody-8M8C2-Immunohistochemistry-Paraffin-NBP3-16322-img0001.jpg)
![Western Blot: TSG101 Antibody [NBP2-98994] - Lane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:43 kDaObserved band size:49 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP2-98994-img0003.jpg "Western Blot: TSG101 Antibody [NBP2-98994] - Lane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:43 kDaObserved band size:49 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-98994] - Immunofluorescence staining of TSG101 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human TSG101 polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98994-img0002.jpg)
![Immunoprecipitation: TSG101 Antibody [NBP2-98994] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell Lysate1 uL anti-TSG101 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TSG101 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size: 44 kDa](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunoprecipitation-NBP2-98994-img0004.jpg)
![Western Blot: VPS4B Antibody [NBP1-19119] - Whole cell lysate from HeLa and 293T cells. VPS4B was also immunoprecipitated by rabbit anti-VPS4B antibody NBP1-19118.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Western-Blot-NBP1-19119-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: VPS4B Antibody [NBP1-19119] - Whole cell lysate from HeLa and 293T cells. VPS4B was also immunoprecipitated by rabbit anti-VPS4B antibody NBP1-19118.")