| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44468 | |||
| BSA Free | NBP2-36766-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-68937-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38774-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44092 | |||
| BSA Free | NBP2-59053-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48786-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-11473-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97465-0.2mg | |||
| BSA Free | NBP1-97465-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50053SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-82364SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25150SS | |||
![Western Blot: ERp57/PDIA3 Antibody (CL2446) [NBP2-36766] - Lane 1: Marker [kDa]. Lane 2:Human cell line U-251 MG](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2446-Western-Blot-NBP2-36766-img0007.jpg "Western Blot: ERp57/PDIA3 Antibody (CL2446) [NBP2-36766] - Lane 1: Marker [kDa]. Lane 2:Human cell line U-251 MG")
![Immunohistochemistry: ERp57/PDIA3 Antibody (CL2446) [NBP2-36766] - Staining of human skeletal muscle shows absence of immunoreactivity.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2446-Immunohistochemistry-NBP2-36766-img0006.jpg)
![Immunohistochemistry: ERp57/PDIA3 Antibody (CL2446) [NBP2-36766] - Staining of human cerebellum shows cytoplasmic immunoreactivity in Purkinje cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2446-Immunohistochemistry-NBP2-36766-img0001.jpg)
![Immunohistochemistry: ERp57/PDIA3 Antibody (CL2446) [NBP2-36766] - Staining of human small intestine shows cytoplasmic positivity in glandular epithelium and lamina propria cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2446-Immunohistochemistry-NBP2-36766-img0002.jpg)
![Immunohistochemistry: ERp57/PDIA3 Antibody (CL2446) [NBP2-36766] - Staining of human fallopian tube shows strong cytoplasmic immunoreactivity in glandular epithelium cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2446-Immunohistochemistry-NBP2-36766-img0003.jpg)
![Immunohistochemistry: ERp57/PDIA3 Antibody (CL2446) [NBP2-36766] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2446-Immunohistochemistry-NBP2-36766-img0004.jpg)
![Immunohistochemistry: ERp57/PDIA3 Antibody (CL2446) [NBP2-36766] - Staining of human placenta shows strong cytoplasmic immunoreactivity in the trophoblast.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2446-Immunohistochemistry-NBP2-36766-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP90 beta Antibody [NBP2-68937] - HSP90AB1 Antibody [NBP2-68937] - Staining of human adrenal gland, cervix, uterine, kidney and testis using Anti-HSP90AB1 antibody NBP2-68937 (A) shows similar protein distribution across tissues to independent antibody NBP2-68978 (B).](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunohistochemistry-Paraffin-NBP2-68937-img0003.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HSP90 beta Antibody [NBP2-68937] - HSP90AB1 Antibody [NBP2-68937] - Staining of human adrenal gland, cervix, uterine, kidney and testis using Anti-HSP90AB1 antibody NBP2-68937 (A) shows similar protein distribution across tissues to independent antibody NBP2-68978 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: HSP90 beta Antibody [NBP2-68937] - HSP90AB1 Antibody [NBP2-68937] - Analysis using Anti-HSP90AB1 antibody NBP2-68937 (A) shows similar pattern to independent antibody NBP2-68978 (B).](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Western-Blot-NBP2-68937-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP90AB1 Antibody [NBP2-68937] - Staining of human cervix, uterine.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunohistochemistry-Paraffin-NBP2-68937-img0007.jpg)
![Immunohistochemistry-Paraffin: HSP90AB1 Antibody [NBP2-68937] - Staining of human kidney.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunohistochemistry-Paraffin-NBP2-68937-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP90AB1 Antibody [NBP2-68937] - Staining of human testis.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunohistochemistry-Paraffin-NBP2-68937-img0006.jpg)
![Immunohistochemistry-Paraffin: HSP90AB1 Antibody [NBP2-68937] - Staining of human adrenal gland.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunohistochemistry-Paraffin-NBP2-68937-img0008.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Analysis in human endometrium and liver tissues. Corresponding CREBBP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Analysis in human endometrium and liver tissues. Corresponding CREBBP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CBP/KAT3A Antibody [NBP2-38774] - Staining of human cell line MCF7 shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38774-img0001.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Staining of human liver shows very weak nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0002.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Staining of human endometrium shows strong nuclear positivity in glandular cells and stromal cells.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0003.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0004.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0005.jpg)
![Western Blot: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Western-Blot-NBP2-59053-img0007.jpg "Western Blot: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Analysis in human cell line RT-4.")
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human skeletal muscle shows only weak cytoplasmic positivity in muscle fibers.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0008.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human cervix shows granular cytoplasmic immunoreactivity in squamous epithelium cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human kidney shows strong granular cytoplasmic positivity in renal tubules and glomeruli.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human rectum shows granular cytoplasmic immunoreactivity in glandular cells and in lymphoid cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human endometrium shows granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous tubules.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp70 interacting protein HIP Antibody [NBP2-48786] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48786-img0004.jpg "Immunocytochemistry/Immunofluorescence: Hsp70 interacting protein HIP Antibody [NBP2-48786] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.")
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-48786] - Staining of human testis.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-48786-img0009.jpg)
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-48786] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-48786-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-48786] - Staining of human colon, kidney, ovary and testis using Anti-ST13 antibody NBP2-48786 (A) shows similar protein distribution across tissues to independent antibody NBP2-47427 (B).](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-48786-img0005.jpg)
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-48786] - Staining of human colon.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-48786-img0006.jpg)
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-48786] - Staining of human kidney.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-48786-img0007.jpg)
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-48786] - Staining of human ovary.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-48786-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERp72 Antibody [NBP2-16371] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using ERP72 antibody (Green) at 1:500 dilution.](http://images.novusbio.com/fullsize/ERp72-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16371-img0004.jpg "Immunocytochemistry/Immunofluorescence: ERp72 Antibody [NBP2-16371] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using ERP72 antibody (Green) at 1:500 dilution.")
![Immunohistochemistry-Paraffin: ERp72 Antibody [NBP2-16371] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ERp72-Antibody-Immunohistochemistry-Paraffin-NBP2-16371-img0003.jpg)
![Western Blot: ERp72 Antibody [NBP2-16371] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ERp72-Antibody-Western-Blot-NBP2-16371-img0001.jpg)
![Western Blot: ERp72 Antibody [NBP2-16371] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/ERp72-Antibody-Western-Blot-NBP2-16371-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ERp72 Antibody [NBP2-16371] - Wild-type (WT) and ERP72 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERp72 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/ERp72-Antibody-Western-Blot-NBP2-16371-img0005.jpg)
![Western Blot: HSP10/EPF Antibody [NB110-11473] - Western blot analysis of Rat brain cell lysates showing detection of HSP10/EPF protein using Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP10-EPF-Antibody-Western-Blot-NB110-11473-img0006.jpg "Western Blot: HSP10/EPF Antibody [NB110-11473] - Western blot analysis of Rat brain cell lysates showing detection of HSP10/EPF protein using Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: HSP10/EPF Antibody [NB110-11473] - Immunohistochemistry analysis using Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis..](http://images.novusbio.com/fullsize/HSP10-EPF Antibody-Immunohistochemistry-NB110-11473-img0007.jpg)
![Western Blot: HSP10/EPF Antibody [NB110-11473] - analysis of Rat brain cell lysates showing detection of Cpn10 protein using Rabbit Anti-Cpn10 Polyclonal Antibody . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP10-EPF-Antibody-Western-Blot-NB110-11473-img0005.jpg)
![Immunohistochemistry: HSP10/EPF Antibody [NB110-11473] - analysis using Rabbit Anti-Cpn10 Polyclonal Antibody . Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative Solution. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis..](http://images.novusbio.com/fullsize/HSP10-EPF-Antibody-Immunohistochemistry-NB110-11473-img0004.jpg)
![Western Blot: CBP/KAT3A Antibody [NB100-382] - Detection of Human CBP by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-382 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Western-Blot-NB100-382-img0008.jpg "Western Blot: CBP/KAT3A Antibody [NB100-382] - Detection of Human CBP by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-382 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry: CBP/KAT3A Antibody [NB100-382] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CBP used at a dilution of 1:500 (2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-NB100-382-img0007.jpg)
![Immunohistochemistry: CBP/KAT3A Antibody [NB100-382] - FFPE section of human prostate adenocarcinoma. Antibody used at a dilution of 1:250. Detection: DAB.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-NB100-382-img0003.jpg)
![Immunoprecipitation: CBP/KAT3A Antibody [NB100-382] - Samples: Nuclear (NE) or cytoplasmic (S100) extract (10 mg) from HeLa cells. Antibody: Affinity purified rabbit anti-CBP used at 20 ug/10 mg extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is CBP.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunoprecipitation-NB100-382-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CBP/KAT3A Antibody [NB100-382] - Detection of human CBP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-382 used for IP at 6 ug/mg lysate. CBP was also immunoprecipitated by rabbit anti-CBP antibody NB100-381. For blotting immunoprecipitated CBP, NB100-382 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunoprecipitation-NB100-382-img0009.jpg)
![Western Blot: FKBP4/FKBP52 Antibody (KN382/EC1) [NBP1-97465] - Analysis of FKBP59 mAb (KN382/EC1): Lane 1: MW marker, Lane 2: HeLa Cell Lysate(heat shocked), Lane 3: MDBK Celll Lysate (heat shocked), Lane 4: VERO Cell Lysate (heat shocked), Lane 5: RK-13 Cell Lysate (heat shocked).](http://images.novusbio.com/fullsize/FKBP4-FKBP52-Antibody-KN382-EC1-Western-Blot-NBP1-97465-img0001.jpg "Western Blot: FKBP4/FKBP52 Antibody (KN382/EC1) [NBP1-97465] - Analysis of FKBP59 mAb (KN382/EC1): Lane 1: MW marker, Lane 2: HeLa Cell Lysate(heat shocked), Lane 3: MDBK Celll Lysate (heat shocked), Lane 4: VERO Cell Lysate (heat shocked), Lane 5: RK-13 Cell Lysate (heat shocked).")
![Western Blot: Calreticulin Antibody (6C6) [NBP2-50053] - Analysis of NBP2-50053 in HEK293 (lane 1), 3T3 (lane 2), SHSY-5Y (lane 3) and HeLa cells (lane 4). NBP2-50053 binds strongly to Calreticulin in 50 kDa.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-6C6-Western-Blot-NBP2-50053-img0002.jpg "Western Blot: Calreticulin Antibody (6C6) [NBP2-50053] - Analysis of NBP2-50053 in HEK293 (lane 1), 3T3 (lane 2), SHSY-5Y (lane 3) and HeLa cells (lane 4). NBP2-50053 binds strongly to Calreticulin in 50 kDa.")
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (6C6) [NBP2-50053] - Analysis of of SH-SY5Y cells stained with mouse mAb to calreticulin, NBP2-50053, dilution 1:500 in green and costained with chicken pAb to lamin A/C, dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA. The MCA-6C6 antibody reveals granular staining of cytoplasm, while the lamin AC antibody stains the nuclear lamina and membrane.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-6C6-Immunocytochemistry-Immunofluorescence-NBP2-50053-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (6C6) [NBP2-50053] - HeLa cell cultures were stained with NBP2-50053 (red). Calreticulin predominately localized in vesicles and the ER. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, green). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-6C6-Immunocytochemistry-Immunofluorescence-NBP2-50053-img0003.jpg)
![Western Blot: TRPM2 Antibody [NB110-82364] - Detection of TRPM2 in mouse brain membrane lysates using NB110-82364.](http://images.novusbio.com/fullsize/TRPM2-Antibody-Western-Blot-NB110-82364-img0002.jpg "Western Blot: TRPM2 Antibody [NB110-82364] - Detection of TRPM2 in mouse brain membrane lysates using NB110-82364.")
![Immunocytochemistry/Immunofluorescence: TRPM2 Antibody [NB110-82364] - TRPM2 antibody was tested in Neuro2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/TRPM2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-82364-img0004.jpg)
![Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-25150-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Analysis of whole cell lysates using chicken pAb to HSP27, NBP2-25150, dilution 1:2,000, in red. [1] protein standard (red), [2] NIH-3T3 , [3] C6, [4] HeLa, [5] HEK293, [6] SH-SY5Y, and [7] COS-1 cells. Strong single band at 27kDa corresponds to the HSP27 protein strongly expressed in human cell lines. Note that the antibody does not recognize rodent HSP27. The blot was simultaneously probed with mouse mAb to Beta-tubulin, dilution 1:10,000 in green. A single band at 50kDa, which is detected in all cell lysates, corresponds to the Beta-tubulin proteins.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP2-25150] - Staining of HeLa cells stained with chicken pAb to heat shock protein 27 (HSP27), NBP2-25150, dilution 1:1,000 in green, and costained with mouse mAb to HSP60, dilution 1:5,000 in red. Blue is DAPI staining of nuclear DNA. NBP2-25150 antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0001.jpg)