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![Western Blot: TCP1 alpha Antibody [NB100-1400] - Staining of Human Ovary (A) lysate and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Western-Blot-NB100-1400-img0004.jpg "Western Blot: TCP1 alpha Antibody [NB100-1400] - Staining of Human Ovary (A) lysate and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: TCP1 alpha Antibody [NB100-1400] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1400-img0005.jpg)
![Immunohistochemistry-Paraffin: TCP1 alpha Antibody [NB100-1400] - Staining of paraffin embedded Human Testis. Antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-1400-img0002.jpg)
![Flow Cytometry: TCP1 alpha Antibody [NB100-1400] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Flow-Cytometry-NB100-1400-img0006.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of HepG2 (A) and NIH3T3 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Western-Blot-NB100-41377-img0006.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of HepG2 (A) and NIH3T3 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Immunocytochemistry analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41377-img0005.jpg)
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunohistochemistry-Paraffin-NB100-41377-img0008.jpg)
![Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 expression in bovine skeletal muscle whole lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49840-img0005.jpg "Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 expression in bovine skeletal muscle whole lysate. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Glut4 Antibody [NBP1-49840] - 3.75ug/ml staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Glut4-Antibody-Immunohistochemistry-Paraffin-NBP1-49840-img0006.jpg)
![Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 in Mouse Heart lysate (35ug protein in RIPA buffer) using NBP1-49840 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49840-img0004.jpg)
![Western Blot: LC3A Antibody (2312D) [NBP2-75924] - Total protein from human HeLa and mouse Neuro2A cells treated with and without 50 uM chloroquine for 24 hours was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LC3A in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/LC3A-Antibody-2312D-Western-Blot-NBP2-75924-img0003.jpg "Western Blot: LC3A Antibody (2312D) [NBP2-75924] - Total protein from human HeLa and mouse Neuro2A cells treated with and without 50 uM chloroquine for 24 hours was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LC3A in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: LC3A Antibody (2312D) [NBP2-75924] - This LC3A antibody was detected in immersion fixed paraffin-embedded sections of human brain (frontal cortex) using Rabbit Anti-Human LC3A Monoclonal Antibody (Catalog # NBP2-75924) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize/LC3A-Antibody-2312D-Immunohistochemistry-Paraffin-NBP2-75924-img0004.jpg)
![Western Blot: Rab3C Antibody [NBP1-32871] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB3C antibody [N1C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Rab3C-Antibody-Western-Blot-NBP1-32871-img0006.jpg "Western Blot: Rab3C Antibody [NBP1-32871] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB3C antibody [N1C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Rab3C Antibody [NBP1-32871] - DLD1 xenograft, using RAB3C antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Rab3C-Antibody-Immunohistochemistry-Paraffin-NBP1-32871-img0002.jpg)
![Western Blot: Rab3C Antibody [NBP1-32871] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/Rab3C-Antibody-Western-Blot-NBP1-32871-img0003.jpg)
![Western Blot: Rab3C Antibody [NBP1-32871] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Rab3C-Antibody-Western-Blot-NBP1-32871-img0004.jpg)
![Rab3C antibody [N1C3] detects Rab3C protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rab3C stained by Rab3C antibody [N1C3] (NBP1-32871) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-32871_rabbit-polyclonal-rab3c-antibody-immunocytochemistry-immunofluorescence-141120251842514.jpg)
![Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FKBP4 antibody.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Western-Blot-NBP1-33702-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FKBP4 antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FKBP52/FKBP4 Antibody [NBP1-33702] - Paraformaldehyde-fixed A431, using (NBP1-33702) antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33702-img0008.jpg)
![Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - A. 30 ug GL261 whole cell lysate/extract, B. 30 ug C8D30 whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Western-Blot-NBP1-33702-img0011.jpg)
![Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - 30 ug Rat 2 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Western-Blot-NBP1-33702-img0012.jpg)
![Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - A. 30 ug 293T whole cell lysate/extract, B. 30 ug A431 whole cell lysate/extract, C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A37C whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Western-Blot-NBP1-33702-img0013.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP2-20700] - Ovarian carcinoma. TRAP1 antibody [N1N3] dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-20700-img0002.jpg "Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP2-20700] - Ovarian carcinoma. TRAP1 antibody [N1N3] dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: TRAP1 Antibody [NBP2-20700] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Western-Blot-NBP2-20700-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody [N1N3] (NBP2-20700) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20700_rabbit-polyclonal-trap1-antibody-western-blot-261120251943819.jpg)
![TRAP1 antibody [N1N3] detects TRAP1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min and permeabilized with 100% MeOH for 30 sec.Green: TRAP1 protein stained by TRAP1 antibody [N1N3] (NBP2-20700) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-20700_rabbit-polyclonal-trap1-antibody-immunocytochemistry-immunofluorescence-261120251832457.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16881] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Western-Blot-NBP2-16881-img0003.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16881] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:10000.")
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16881] - Analysis of paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Immunohistochemistry-Paraffin-NBP2-16881-img0004.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16881] - Sample (20 ug of whole cell lysate) A: Raw264. 7 NBP2-16881 diluted at 1:2000.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Western-Blot-NBP2-16881-img0001.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16881] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Western-Blot-NBP2-16881-img0002.jpg)
![Western Blot: PTGES3 Antibody [NBP2-19999] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PTGES3-Antibody-Western-Blot-NBP2-19999-img0002.jpg "Western Blot: PTGES3 Antibody [NBP2-19999] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PTGES3 Antibody [NBP2-19999] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PTGES3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19999-img0004.jpg)
![Immunohistochemistry-Paraffin: p23/PTGES3 Antibody [NBP2-19999] - p23/PTGES3 antibody detects p23 protein at cytoplasm in mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. p23 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-Immunohistochemistry-Paraffin-NBP2-19999-img0005.jpg)
![Western Blot: PTGES3 Antibody [NBP2-19999] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PTGES3-Antibody-Western-Blot-NBP2-19999-img0001.jpg)
![Immunohistochemistry-Paraffin: PTGES3 Antibody [NBP2-19999] - Immunohistochemical analysis of paraffin-embedded HT-29 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PTGES3-Antibody-Immunohistochemistry-Paraffin-NBP2-19999-img0003.jpg)
![Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 ug. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Western-Blot-NBP2-59339-img0004.jpg "Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 ug. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis on non-permeabilized HCT116 cells using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate showing cell membrane staining. Tissue: HCT116 cells. Species: Human. Fixation: 4% Formaldehyde. Permeabilization: None. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100. Counterstain: Wheat germ agglutinin Texas red membrane marker; DAPI (blue) nuclear stain. Localization: Cell surface, cell membrane. (A) DAPI nuclear stain. (B) Wheat germ agglutinin Texas red. (C) HSP70 Antibody. (D) Composite. Courtesy of: Lawrence Hightower, Charles Giardina, and Didem Ozcan from University of Connecticut.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Immunocytochemistry-Immunofluorescence-NBP2-59339-img0006.jpg)
![Flow (Cell Surface): HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - A cell surface stain was performed on HeLa cells with Hsp70 / Hsc70 Antibody (1H11) NBP2-59339PE (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Flow-Cell-Surface-NBP2-59339-img0007.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Hsp70 / Hsc70 Antibody (1H11) [NBP2-59339] - Clone 1H11: FITC conjugate. Tissue: Jurkat E6.1. Species: Human. Unfixed/non-permeablized cells were incubated with (A) Anti-HSP70 control antibody (not cell-surface-specific), or (B) Anti-HSP70 antibody, clone 1H11 for 40 min at 4 degrees C. Fixed/permeabilized cells were incubated with (C) Anti-HSP70 control antibody, or (D) Anti-HSP70 antibody, clone 1H11 or 40 min at 4 degrees C. Analysis shows that non-permeabilized cells show cell surface binding with 1H11 clone (NBP2-59339) but not with the control antibody, and the permeabilized cells show strong intracellular binding with the control antibody but much less with 1H11 clone (NBP2-59339). Courtesy of: Dr. Elyse Ireland, Institute of Medicine, University of Chester.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Flow-Cytometry-NBP2-59339-img0002.jpg)