| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84675-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43999 | |||
| BSA Free | NBP2-59780-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90788-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96425-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97872-0.2mg | |||
| BSA Free | NBP1-97872-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48051 | |||
| Azide and BSA Free | NBP3-11500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24698SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-48002-0.1mg | |||
| IHC-Prediluted | NBP2-48186 | |||
![Immunohistochemistry-Paraffin: FKBP52/FKBP4 Antibody [NBP1-84675] - Staining in human testis and duodenum tissues using anti-FKBP4 antibody. Corresponding FKBP4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Immunohistochemistry-Paraffin-NBP1-84675-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FKBP52/FKBP4 Antibody [NBP1-84675] - Staining in human testis and duodenum tissues using anti-FKBP4 antibody. Corresponding FKBP4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: FKBP52/FKBP4 Antibody [NBP1-84675] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Western-Blot-NBP1-84675-img0007.jpg)
![Immunohistochemistry-Paraffin: FKBP52/FKBP4 Antibody [NBP1-84675] - Staining of human duodenum shows low expression as expected.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Immunohistochemistry-Paraffin-NBP1-84675-img0010.jpg)
![Immunohistochemistry-Paraffin: FKBP52/FKBP4 Antibody [NBP1-84675] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Immunohistochemistry-Paraffin-NBP1-84675-img0011.jpg)
![Western Blot: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Western-Blot-NBP2-59780-img0009.jpg "Western Blot: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line RT-4")
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human skeletal muscle shows only very low cytoplasmic immunoreactivity.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Immunohistochemistry-Paraffin-NBP2-59780-img0005.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human liver shows strong granular cytoplasmic immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Immunohistochemistry-Paraffin-NBP2-59780-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human fallopian tube shows granular cytoplasmic positivity in epithelial cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Immunohistochemistry-Paraffin-NBP2-59780-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human endometrium shows granular cytoplasmic immunoreactivity in epithelial and stromal cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Immunohistochemistry-Paraffin-NBP2-59780-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human prostate shows strong granular cytoplasmic positivity in epithelial cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Immunohistochemistry-Paraffin-NBP2-59780-img0004.jpg)
![Western Blot: DENND4C Antibody [NBP1-90788] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/DENND4C-Antibody-Western-Blot-NBP1-90788-img0007.jpg "Western Blot: DENND4C Antibody [NBP1-90788] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: DENND4C Antibody [NBP1-90788] - Staining of human cell line A-431 shows localization to cytosol, the Golgi apparatus & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DENND4C-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90788-img0008.jpg)
![Western Blot: DENND4C Antibody [NBP1-90788] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/DENND4C-Antibody-Western-Blot-NBP1-90788-img0006.jpg)
![Western Blot: HSP70/HSPA1A Antibody (N27F3-4) [NB110-96425] - analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-N27F3-4-Western-Blot-NB110-96425-img0004.jpg "Western Blot: HSP70/HSPA1A Antibody (N27F3-4) [NB110-96425] - analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: HSP70/HSPA1A Antibody (N27F3-4) [NB110-96425] - Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27F3-4 (NB110-96425). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (NB110-96425) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-N27F3-4-Immunohistochemistry-NB110-96425-img0007.jpg)
![Western Blot: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERp72 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERp72.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Western-Blot-NBP2-71488-img0002.jpg "Western Blot: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERp72 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERp72.")
![Immunohistochemistry: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - Staining of paraffin-embedded Human pancreas tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71488-img0008.jpg)
![Western Blot: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ERp72 monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Western-Blot-NBP2-71488-img0001.jpg)
![Immunohistochemistry: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71488-img0003.jpg)
![Immunohistochemistry: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71488-img0004.jpg)
![Immunohistochemistry: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71488-img0005.jpg)
![Immunohistochemistry: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - Staining of paraffin-embedded Human liver tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71488-img0006.jpg)
![Immunohistochemistry: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - Staining of paraffin-embedded Human lymphoma tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71488-img0007.jpg)
![Immunohistochemistry: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - Staining of paraffin-embedded Human tonsil using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71488-img0009.jpg)
![Western Blot: Hsp70 / Hsc70 Antibody [NBP1-97872] - Analysis of HSC70/HSP70, pAb Lane 1: MW Marker , Lane 2: 3T3 Cell Lysate Lane 3: 3T3 Cell Lysate (heat shocked) Lane 4: PC-12 Cell Lysate , Lane 5: PC-12 Cell Lysate (heat shocked) Lane 6: HeLa Cell Lysate Lane 7: HeLa Cell Lysate (heat shocked), Lane 8: HSP70/HSP72 (human), (recombinant) Lane 9: HSC70/HSP73 (bovine), (recombinant).](http://images.novusbio.com/fullsize/Hsp70---Hsc70-Antibody-Western-Blot-NBP1-97872-img0003.jpg "Western Blot: Hsp70 / Hsc70 Antibody [NBP1-97872] - Analysis of HSC70/HSP70, pAb Lane 1: MW Marker , Lane 2: 3T3 Cell Lysate Lane 3: 3T3 Cell Lysate (heat shocked) Lane 4: PC-12 Cell Lysate , Lane 5: PC-12 Cell Lysate (heat shocked) Lane 6: HeLa Cell Lysate Lane 7: HeLa Cell Lysate (heat shocked), Lane 8: HSP70/HSP72 (human), (recombinant) Lane 9: HSC70/HSP73 (bovine), (recombinant).")
![Immunohistochemistry-Paraffin: HSP70 / HSC70 Antibody [NBP1-97872] - Analysis of human spleen, neutrophils tissue stained with HSC70/HSP70, pAb at 10ug/ml.](http://images.novusbio.com/fullsize/HSP70---HSC70-Antibody-Immunohistochemistry-Paraffin-NBP1-97872-img0001.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (SPM249) [NBP2-44690] - Human Breast Carcinoma stained with GRP94 Monoclonal Antibody (SPM249).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-SPM249-Immunohistochemistry-Paraffin-NBP2-44690-img0001.jpg "Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (SPM249) [NBP2-44690] - Human Breast Carcinoma stained with GRP94 Monoclonal Antibody (SPM249).")
![Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (SPM249) [NBP2-44690] - Flow Cytometric Analysis of PFA-fixed HePG2 cells using gp96/HSP90B1/GRP94 Antibody (SPM249).followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-SPM249-Flow-Cytometry-NBP2-44690-img0002.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody (SPM249) [NBP2-44690] - Classical swine fever virus (CSFV) infection induces the activation of UPR. (A) RNA extracted from CSFV-infected cells was quantified for the expression of UPR genes Xbp1(s), GRP78, GRP94, EDEM-1, ATF4, ATF6, CHOP, Calreticulin, Calnexin, & ERp57 using q-PCR. Mock-infected PK-15 & Thapsigargin (TG)-treated PK-15 were used as negative & positive controls, respectively, & the fold induction was calculated compared to mock cells at the same time point. Error bars represent the mean ± SD of 3 independent experiments; one-way ANOVA test; ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001. (B,D,E) Immunoblotting analysis of components of UPR signaling pathways during a time course of CSFV infection. Mock or CSFV-infected PK-15 cells lysates were collected at the indicated time points. Lysates were analyzed for the activation of the IRE1 (B), PERK (D) & ATF6 (E) pathway by immunoblotting analysis. Tubulin was used as a loading control, & infection was confirmed by detecting the viral protein Npro. Results of a representative experiment of 2 independent experiments are shown. (C) RNA was collected as described above, & the splicing levels of XBP1 were analyzed with semi-quantitative PCR as described in materials & methods. The length of Xbp1(u) is 474 bp & Xbp1(s) is 448 bp. (F) The relative expression ratios of the targeted proteins/genes were analyzed by densitometric scanning. Error bars represent the mean ± SD of 2 independent experiments; one-way ANOVA test; ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fmicb.2017.02129/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-44690_rat-monoclonal-gp96-hsp90b1-grp94-antibody-spm249-31020241612223.jpg)
![Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody [C3], C-term diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Western-Blot-NB100-78614-img0022.jpg "Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody [C3], C-term diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody [NB100-78614] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERp57 protein stained by ERp57 antibody [C3], C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-78614-img0019.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - Paraffin-embedded mouse lymph node. ERp57 antibody [C3], C-term dilution: 1:500.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NB100-78614-img0021.jpg)
![Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Western-Blot-NB100-78614-img0013.jpg)
![Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Hep G2 7.5% SDS PAGE diluted at 1:10000](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Western-Blot-NB100-78614-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody [NB100-78614] - Sample: MCF-7 cells were fixed in iced-cold MeOH for 5 min. Green: PDIA3 protein stained by ERp57 antibody, C-term diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-78614-img0011.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - H1299 xenograft, using ERp57 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NB100-78614-img0012.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - Mouse intestine. ERp57 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NB100-78614-img0016.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - Rat middle brain. ERp57 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NB100-78614-img0017.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP2-24698] - Analysis of human GRP94 in 3T3 cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24698 at 1 ug/ml.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Western-Blot-NBP2-24698-img0006.jpg "Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP2-24698] - Analysis of human GRP94 in 3T3 cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24698 at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody [NBP2-24698] - GPR94 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24698-img0004.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP2-24698] - Analysis of Human Placenta using NBP2-24698 at 10 ug/ml.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP2-24698-img0005.jpg)
![Western Blot: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Western Blot Analysis of HeLa cell lysate using HSP27 MAb (HSPB1/774). Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Western-Blot-NBP2-44975-img0006.jpg "Western Blot: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Western Blot Analysis of HeLa cell lysate using HSP27 MAb (HSPB1/774). Image using the Azide and BSA Free format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (HSPB1/774) [NBP2-44975] - ICC/IF analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Immunofluorescence-NBP2-44975-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Human Prostate Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Immunohistochemistry-Paraffin-NBP2-44975-img0002.jpg)
![Flow Cytometry: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (HSPB1/774) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Flow-Cytometry-NBP2-44975-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Human Breast Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Immunohistochemistry-Paraffin-NBP2-44975-img0001.jpg)
![Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-24533-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Analysis of HSP27 in human skeletal muscle lysate using 1) preimmune sera at 1:5000 and 2) peptide affinity purified HSP27 antibody at 2.0 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP2-24533-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NBP2-24533] - FFPE human small intestine probed with HSP27 antibody at 10 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-Paraffin-NBP2-24533-img0002.jpg)