| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12878-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96431-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72607 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-22440-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22214-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44974 | |||
| Azide and BSA Free | NBP2-34751-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97927-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12880-50ug | |||
![Western Blot: HSP90 Antibody (4F3.E8) [NBP2-12878] - Western Blot analysis of Rat tissue lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F3-E8-Western-Blot-NBP2-12878-img0011.jpg "Western Blot: HSP90 Antibody (4F3.E8) [NBP2-12878] - Western Blot analysis of Rat tissue lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (4F3.E8) [NBP2-12878] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F3-E8-Immunocytochemistry-Immunofluorescence-NBP2-12878-img0007.jpg)
![Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F3-E8-Immunohistochemistry-NBP2-12878-img0014.jpg)
![Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:100000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F3-E8-Immunohistochemistry-NBP2-12878-img0012.jpg)
![Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Tissue: muscle tissue. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F3-E8-Immunohistochemistry-NBP2-12878-img0013.jpg)
![Western Blot: HSP27 Antibody (8A7) [NB110-96431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:8,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NB110-96431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Knockout-Validated-NB110-96431-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (8A7) [NB110-96431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:8,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NB110-96431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (8A7) [NB110-96431] - analysis of Rat Lung tissue lysates showing detection of Hsp27 protein using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 . Load: 15 ug protein. Block: 5% blocking solution. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Western-Blot-NB110-96431-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (8A7) [NB110-96431] - analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 . Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Courtesy of: Mike Welsh, Umich.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunocytochemistry-Immunofluorescence-NB110-96431-img0007.jpg)
![Immunohistochemistry: HSP27 Antibody (8A7) [NB110-96431] - Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (NB110-96431). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (NB110-96431) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-NB110-96431-img0016.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (8A7) [NB110-96431] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-Paraffin-NB110-96431-img0008.jpg)
![Immunohistochemistry: HSP27 Antibody (8A7) [NB110-96431] - Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (NB110-96431). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (NB110-96431) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-NB110-96431-img0017.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0015.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0001.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0002.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0003.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0004.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0005.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0006.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human lung tissue within the normal limits using ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0007.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0008.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0009.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0010.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0011.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0012.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0013.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0014.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0016.jpg)
![Western Blot: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of 50ug of the indicated whole cell lysates and 15ul of PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Western-Blot-NBP2-22440-img0015.jpg "Western Blot: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of 50ug of the indicated whole cell lysates and 15ul of PageRuler Prestained Protein Ladder.")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 (HSP60) (green) in HeLa and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP60 monoclonal antibody, at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0014.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) [NBP2-22440] - Normal deparaffinized human Tonsil tissue.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunohistochemistry-Paraffin-NBP2-22440-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 using Anti-Heat Shock Protein 60 Monoclonal Antibody (4B9/89) shows staining in A2058 Cells. Heat Shock Protein 60 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 60 at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 using Anti-Heat Shock Protein 60 Monoclonal Antibody (4B9/89) shows staining in ATDC5 Cells. Heat Shock Protein 60 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 60 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 using Anti-Heat Shock Protein 60 Monoclonal Antibody (4B9/89) shows staining in Hela Cells. Heat Shock Protein 60 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 60 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 using a Hsp60 Monoclonal Antibody in human endothelial cells.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 (Hsp60, green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with (left panel) or without (right panel) a Hsp60 monoclonal antibody, at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with Dylight 554 phalloidin, and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0013.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) [NBP2-22440] - Cancer biopsies of deparaffinized human Breast carcinoma tissue.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunohistochemistry-Paraffin-NBP2-22440-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) [NBP2-22440] - Normal deparaffinized human Kidney tissue.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunohistochemistry-Paraffin-NBP2-22440-img0004.jpg)
![Immunoprecipitation: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 (HSP60) was performed on HeLa cells. Antigen:antibody complexes were formed by incubating 500ug whole cell lysate with 2ug of HSP60 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent (HRP) at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunoprecipitation-NBP2-22440-img0002.jpg)
![Western Blot: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP 60) was performed by loading 50ug of the indicated whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a concentration of 1ug/ml overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM secondary antibody at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Western-Blot-NB120-5479-img0016.jpg "Western Blot: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP 60) was performed by loading 50ug of the indicated whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a concentration of 1ug/ml overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM secondary antibody at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP60) (green) in HeLa and NIH3T3 cells.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0013.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (2E1/53) [NB120-5479] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunohistochemistry-Paraffin-NB120-5479-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Anlysis of Hela Cells at a dilution of 1:200](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Heat Shock Protein 60 monoclonal antibody (2E1/53) at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Heat Shock Protein 60 monoclonal antibody (2E1/53) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0012.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (2E1/53) [NB120-5479] - Both normal and cancer biopsies of deparaffinized Human kidney tissue tissues.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunohistochemistry-Paraffin-NB120-5479-img0014.jpg)
![Immunoprecipitation: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP60) was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 2ug of a HSP60 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a concentration of 1ug/ml overnight rotating at 4C, washed in TBST, and probed with a goat anti-mouse IgM secondary antibody at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunoprecipitation-NB120-5479-img0006.jpg)
![Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter GLUT4 mouse mAb against HeLa (1), NIH3T3 (2), 3T3-L1 (3) cell lysate and Mouse heart (4) tissue lysate.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Western-Blot-NBP2-22214-img0008.jpg "Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter GLUT4 mouse mAb against HeLa (1), NIH3T3 (2), 3T3-L1 (3) cell lysate and Mouse heart (4) tissue lysate.")
![Immunocytochemistry/Immunofluorescence: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of HepG2 cells using Glucose Transporter GLUT4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Immunocytochemistry-Immunofluorescence-NBP2-22214-img0003.jpg)
![Immunohistochemistry-Paraffin: Glut4 Antibody (3G10A3) [NBP2-22214] - analysis of cardiac muscle tissues using SLC2A4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Immunohistochemistry-Paraffin-NBP2-22214-img0009.jpg)
![Flow Cytometry: Glut4 Antibody (3G10A3) [NBP2-22214] - Flow cytometric analysis of HeLa cells using Glucose Transporter GLUT4 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Flow-Cytometry-NBP2-22214-img0001.jpg)
![Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter Glut4 Antibody (3G10A3)mAb against HEK293 (1) and Glucose Transporter GLUT4 (AA: 224-353)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Western-Blot-NBP2-22214-img0006.jpg)
![Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - human Glucose Transporter GLUT4 recombinant protein.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Western-Blot-NBP2-22214-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of HeLa cells using Glucose Transporter GLUT4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Immunocytochemistry-Immunofluorescence-NBP2-22214-img0002.jpg)
![Immunohistochemistry-Paraffin: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of paraffin-embedded bladder cancer tissues using Glucose Transporter GLUT4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Immunohistochemistry-Paraffin-NBP2-22214-img0004.jpg)
![ELISA: Glut4 Antibody (3G10A3) [NBP2-22214] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-ELISA-NBP2-22214-img0005.jpg)
![Western Blot: HSP27 Antibody (SPM252) [NBP2-32865] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (SPM252).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Western-Blot-NBP2-32865-img0005.jpg "Western Blot: HSP27 Antibody (SPM252) [NBP2-32865] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (SPM252).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (SPM252) [NBP2-32865] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunocytochemistry-Immunofluorescence-NBP2-32865-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 Mouse Monoclonal Antibody (SPM252).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0006.jpg)
![Flow Cytometry: HSP27 Antibody (SPM252) [NBP2-32865] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (SPM252).followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Flow-Cytometry-NBP2-32865-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-paraffin breast carcinoma (20X) stained with HSP27 MAb (SPM252)](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0002.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-paraffin breast carcinoma (10X) stained with HSP27 MAb (SPM252))](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0001.jpg)
![Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 expression in bovine skeletal muscle whole lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49840-img0005.jpg "Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 expression in bovine skeletal muscle whole lysate. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Glut4 Antibody [NBP1-49840] - 3.75ug/ml staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Glut4-Antibody-Immunohistochemistry-Paraffin-NBP1-49840-img0006.jpg)
![Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 in Mouse Heart lysate (35ug protein in RIPA buffer) using NBP1-49840 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49840-img0004.jpg)
![Western Blot: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Analysis using Anti-ST13 antibody NBP2-47427 (A) shows similar pattern to independent antibody NBP2-48865 (B).](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Western-Blot-NBP2-47427-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Analysis using Anti-ST13 antibody NBP2-47427 (A) shows similar pattern to independent antibody NBP2-48865 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47427-img0004.jpg)
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human kidney.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-47427-img0009.jpg)
![Immunohistochemistry: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-NBP2-47427-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human colon, kidney, ovary and testis using Anti-ST13 antibody NBP2-47427 (A) shows similar protein distribution across tissues to independent antibody NBP2-48786 (B).](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-47427-img0005.jpg)
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human testis.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-47427-img0007.jpg)
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human colon.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-47427-img0008.jpg)
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human ovary.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-47427-img0010.jpg)
![Western Blot: Aquaporin-3 Antibody [NBP1-97927] - Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin-3 protein using Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927). Primary Antibody: Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927) at 1:2000.](http://images.novusbio.com/fullsize/Aquaporin-3-Antibody-Western-Blot-NBP1-97927-img0007.jpg "Western Blot: Aquaporin-3 Antibody [NBP1-97927] - Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin-3 protein using Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927). Primary Antibody: Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927) at 1:2000.")
![Immunohistochemistry: Aquaporin-3 Antibody [NBP1-97927] - Immunohistochemistry analysis using Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 m cryostat sections.](http://images.novusbio.com/fullsize/Aquaporin-3 Antibody-Immunohistochemistry-NBP1-97927-img0008.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Western Blot analysis of Human Cell lysates showing detection of HSPA8/HSC71/Hsc70 protein using Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody, Clone 1F2-H5 (NBP2-12880). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody (NBP2-12880) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Western-Blot-NBP2-12880-img0010.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Western Blot analysis of Human Cell lysates showing detection of HSPA8/HSC71/Hsc70 protein using Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody, Clone 1F2-H5 (NBP2-12880). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody (NBP2-12880) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein.")
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 Monoclonal Antibody, Clone 1F2-H5. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degrees C. Primary Antibody: Mouse Anti-Hsc70 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Bright cytoplasmic staining, duller nuclear staining.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Immunocytochemistry-Immunofluorescence-NBP2-12880-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody, Clone 1F2-H5. Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Localizes to nucleus upon heat shock. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsc70 (Hsp73) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Immunocytochemistry-Immunofluorescence-NBP2-12880-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody, Clone 1F2-H5. Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Localizes to nucleus upon heat shock. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsc70 (Hsp73) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Immunocytochemistry-Immunofluorescence-NBP2-12880-img0007.jpg)
![Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NBP1-87712] - Staining in human testis and skeletal muscle tissues . Corresponding KPNB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Importin-beta-KPNB1-Antibody-Immunohistochemistry-Paraffin-NBP1-87712-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NBP1-87712] - Staining in human testis and skeletal muscle tissues . Corresponding KPNB1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Importin beta/KPNB1 Antibody [NBP1-87712] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Importin-beta-KPNB1-Antibody-Western-Blot-NBP1-87712-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Importin beta/KPNB1 Antibody [NBP1-87712] - Staining of human cell line A-431 shows localization to nucleoplasm, nuclear membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Importin-beta-KPNB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87712-img0010.jpg)
![Western Blot: Importin beta/KPNB1 Antibody [NBP1-87712] - Analysis in human cell line A-549.](http://images.novusbio.com/fullsize/Importin-beta-KPNB1-Antibody-Western-Blot-NBP1-87712-img0007.jpg)
![Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NBP1-87712] - Staining of human cerebral cortex shows moderate to strong nuclear and nuclear membrane positivity in neurons.](http://images.novusbio.com/fullsize/Importin-beta-KPNB1-Antibody-Immunohistochemistry-Paraffin-NBP1-87712-img0015.jpg)
![Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NBP1-87712] - Staining of human skeletal muscle shows very weak nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/Importin-beta-KPNB1-Antibody-Immunohistochemistry-Paraffin-NBP1-87712-img0017.jpg)
![Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NBP1-87712] - Staining of human stomach shows moderate nuclear and nuclear membrane positivity in glandular cells.](http://images.novusbio.com/fullsize/Importin-beta-KPNB1-Antibody-Immunohistochemistry-Paraffin-NBP1-87712-img0018.jpg)
![Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NBP1-87712] - Staining of human testis shows moderate to strong nuclear and nuclear membrane positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Importin-beta-KPNB1-Antibody-Immunohistochemistry-Paraffin-NBP1-87712-img0019.jpg)