| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44974 | |||
| Azide and BSA Free | NBP2-34751-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25154SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12880-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97927-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47773-0.1mg | |||
| IHC-Prediluted | NBP2-48118 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-115SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44499 | |||
| Azide and BSA Free | NBP2-34742-0.1mg | |||
![Western Blot: HSP27 Antibody (SPM252) [NBP2-32865] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (SPM252).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Western-Blot-NBP2-32865-img0005.jpg "Western Blot: HSP27 Antibody (SPM252) [NBP2-32865] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (SPM252).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (SPM252) [NBP2-32865] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunocytochemistry-Immunofluorescence-NBP2-32865-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 Mouse Monoclonal Antibody (SPM252).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0006.jpg)
![Flow Cytometry: HSP27 Antibody (SPM252) [NBP2-32865] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (SPM252).followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Flow-Cytometry-NBP2-32865-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-paraffin breast carcinoma (20X) stained with HSP27 MAb (SPM252)](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0002.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-paraffin breast carcinoma (10X) stained with HSP27 MAb (SPM252))](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of HeLa cells stained with mouse LAMP-1/CD107a mAb, dilution 1:500 (Red), and costained with chicken Vimentin pAb, dilution 1:10000, (Green). DAPI staining of nuclear DNA (Blue). The cells were treated with 50uM of chloroquine, an inhibitor of autophagy, for 16hrs prior to staining. The LAMP-1/CD107a antibody reveals vesicular staining of LAMP1 protein accumulated in swollen lysosomes, while the Vimentin antibody specifically labels the intermediate filament network in these cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5H6-Immunocytochemistry-Immunofluorescence-NBP2-25154-img0007.jpg "Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of HeLa cells stained with mouse LAMP-1/CD107a mAb, dilution 1:500 (Red), and costained with chicken Vimentin pAb, dilution 1:10000, (Green). DAPI staining of nuclear DNA (Blue). The cells were treated with 50uM of chloroquine, an inhibitor of autophagy, for 16hrs prior to staining. The LAMP-1/CD107a antibody reveals vesicular staining of LAMP1 protein accumulated in swollen lysosomes, while the Vimentin antibody specifically labels the intermediate filament network in these cells.")
![Western Blot: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of different cell lysates using mouse mAb to LAMP1, NBP2-25154 dilution 1:10,000 in green. Cells were maintained under normal conditions (Ct), or treated with 50uM of chloroquine (CQ), an inhibitor of autophagy, for 24 hours: [1] protein standard (red), [2] HeLa Ct, [3] HeLa+CQ, [4] NIH-3T3 Ct, and [5] NIH-3T3+CQ. The smeared band between 75-120kDa corresponds to variably glycosylated forms of the LAMP1 protein detected only in the human cells, this antibody fails to recognize the rodent homologue. The same blot was probed with chicken pAB to HSP60, dilution 1:20,000. The HSP60 antibody reveals heat shock 60 protein with apparent molecular weight of 60kDa in all preparations.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5H6-Western-Blot-NBP2-25154-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - D2.0 R cells activate autophagy upon entering a dormant state. Representative images are shown for immunofluorescent staining of lysosomal (LAMP-1/CD107a, red) and autophagic (LC3, green) markers of D2.0 R cells in BME (upper panels) and BME + COL1 (lower panels). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04070-6), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5H6-Immunocytochemistry-Immunofluorescence-NBP2-25154-img0008.jpg)
![Western Blot: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Analysis using Anti-ST13 antibody NBP2-47427 (A) shows similar pattern to independent antibody NBP2-48865 (B).](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Western-Blot-NBP2-47427-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Analysis using Anti-ST13 antibody NBP2-47427 (A) shows similar pattern to independent antibody NBP2-48865 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47427-img0004.jpg)
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human kidney.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-47427-img0009.jpg)
![Immunohistochemistry: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-NBP2-47427-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human colon, kidney, ovary and testis using Anti-ST13 antibody NBP2-47427 (A) shows similar protein distribution across tissues to independent antibody NBP2-48786 (B).](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-47427-img0005.jpg)
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human testis.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-47427-img0007.jpg)
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human colon.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-47427-img0008.jpg)
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human ovary.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-47427-img0010.jpg)
![Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 expression in bovine skeletal muscle whole lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49840-img0005.jpg "Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 expression in bovine skeletal muscle whole lysate. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Glut4 Antibody [NBP1-49840] - 3.75ug/ml staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Glut4-Antibody-Immunohistochemistry-Paraffin-NBP1-49840-img0006.jpg)
![Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 in Mouse Heart lysate (35ug protein in RIPA buffer) using NBP1-49840 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49840-img0004.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Western Blot analysis of Human Cell lysates showing detection of HSPA8/HSC71/Hsc70 protein using Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody, Clone 1F2-H5 (NBP2-12880). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody (NBP2-12880) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Western-Blot-NBP2-12880-img0010.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Western Blot analysis of Human Cell lysates showing detection of HSPA8/HSC71/Hsc70 protein using Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody, Clone 1F2-H5 (NBP2-12880). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody (NBP2-12880) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein.")
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 Monoclonal Antibody, Clone 1F2-H5. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degrees C. Primary Antibody: Mouse Anti-Hsc70 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Bright cytoplasmic staining, duller nuclear staining.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Immunocytochemistry-Immunofluorescence-NBP2-12880-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody, Clone 1F2-H5. Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Localizes to nucleus upon heat shock. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsc70 (Hsp73) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Immunocytochemistry-Immunofluorescence-NBP2-12880-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody, Clone 1F2-H5. Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Localizes to nucleus upon heat shock. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsc70 (Hsp73) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Immunocytochemistry-Immunofluorescence-NBP2-12880-img0007.jpg)
![Western Blot: Aquaporin-3 Antibody [NBP1-97927] - Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin-3 protein using Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927). Primary Antibody: Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927) at 1:2000.](http://images.novusbio.com/fullsize/Aquaporin-3-Antibody-Western-Blot-NBP1-97927-img0007.jpg "Western Blot: Aquaporin-3 Antibody [NBP1-97927] - Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin-3 protein using Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927). Primary Antibody: Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927) at 1:2000.")
![Immunohistochemistry: Aquaporin-3 Antibody [NBP1-97927] - Immunohistochemistry analysis using Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 m cryostat sections.](http://images.novusbio.com/fullsize/Aquaporin-3 Antibody-Immunohistochemistry-NBP1-97927-img0008.jpg)
![Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Lung Carcinoma stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)](http://images.novusbio.com/fullsize/TTF-1-NKX2-1-Antibody-8G7G3-1-+-NX2-1-690-Immunohistochemistry-Paraffin-NBP2-44501-img0001.jpg "Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Lung Carcinoma stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)")
![Immunohistochemistry: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - TTF1 staining in thyroid tissue of wild-type C57 mouse (red). Dilution is 1:100. Image from a verified customer review.](http://images.novusbio.com/fullsize/TTF-1-NKX2-1-Antibody-8G7G3-1-+-NX2-1-690-Immunohistochemistry-NBP2-44501-img0003.jpg)
![Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Thyroid stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)](http://images.novusbio.com/fullsize/TTF-1-NKX2-1-Antibody-8G7G3-1-+-NX2-1-690-Immunohistochemistry-Paraffin-NBP2-44501-img0002.jpg)
![Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NBP1-87712] - Staining in human testis and skeletal muscle tissues . Corresponding KPNB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Importin-beta-KPNB1-Antibody-Immunohistochemistry-Paraffin-NBP1-87712-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NBP1-87712] - Staining in human testis and skeletal muscle tissues . Corresponding KPNB1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Importin beta/KPNB1 Antibody [NBP1-87712] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Importin-beta-KPNB1-Antibody-Western-Blot-NBP1-87712-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Importin beta/KPNB1 Antibody [NBP1-87712] - Staining of human cell line A-431 shows localization to nucleoplasm, nuclear membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Importin-beta-KPNB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87712-img0010.jpg)
![Western Blot: Importin beta/KPNB1 Antibody [NBP1-87712] - Analysis in human cell line A-549.](http://images.novusbio.com/fullsize/Importin-beta-KPNB1-Antibody-Western-Blot-NBP1-87712-img0007.jpg)
![Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NBP1-87712] - Staining of human cerebral cortex shows moderate to strong nuclear and nuclear membrane positivity in neurons.](http://images.novusbio.com/fullsize/Importin-beta-KPNB1-Antibody-Immunohistochemistry-Paraffin-NBP1-87712-img0015.jpg)
![Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NBP1-87712] - Staining of human skeletal muscle shows very weak nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/Importin-beta-KPNB1-Antibody-Immunohistochemistry-Paraffin-NBP1-87712-img0017.jpg)
![Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NBP1-87712] - Staining of human stomach shows moderate nuclear and nuclear membrane positivity in glandular cells.](http://images.novusbio.com/fullsize/Importin-beta-KPNB1-Antibody-Immunohistochemistry-Paraffin-NBP1-87712-img0018.jpg)
![Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NBP1-87712] - Staining of human testis shows moderate to strong nuclear and nuclear membrane positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Importin-beta-KPNB1-Antibody-Immunohistochemistry-Paraffin-NBP1-87712-img0019.jpg)
![Western Blot: ABCF2 Antibody [NB400-115] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and ABCF2 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human ABCF2 Polyclonal Antibody (Catalog # NB400-115) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for ABCF2 at approximately 71 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Knockdown-Validated-NB400-115-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ABCF2 Antibody [NB400-115] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and ABCF2 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human ABCF2 Polyclonal Antibody (Catalog # NB400-115) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for ABCF2 at approximately 71 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: ABCF2 Antibody [NB400-115] - Analysis of ABCF2 on Lane 1: HeLa whole cell extracts and Lane 2: NIH/3T3 cell lysates using NB400-115.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Western-Blot-NB400-115-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ABCF2 Antibody [NB400-115] - ABCF2 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunocytochemistry-Immunofluorescence-NB400-115-img0005.jpg)
![Simple Western: ABCF2 Antibody [NB400-115] - Simple Western lane view shows a specific band for ABCF2 in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Simple-Western-NB400-115-img0006.jpg)
![Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Knockout-Validated-NBP2-25149-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of tissue or whole cell lysates using mouse mAb to HSP27, NBP2-25149, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong single band at ~27 kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific and unreactive with rodent HSP27. The blot was simultaneously probed with rabbit pAb to HSP-60, dilution 1:5,000 in red. A strong 60 kDa band is present in all preparations.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Western-Blot-NBP2-25149-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of HeLa cells stained with mouse HSP27 mAb, dilution 1:2000 (Green), and costained with chicken HSP60 pAb, dilution 1:2000 (Red). DAPI staining of nuclear DNA (Blue). The HSP27 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0008.jpg)
![Flow Cytometry: Hsp27 Antibody (6H11) [NBP2-25149] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Flow-Cytometry-NBP2-25149-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp27 Antibody (6H11) [NBP2-25149] - HeLa cells stained with NBP2-25149 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25149 reveals strong cytoplasmic staining and penetrates into the Actin rich ruffled margins, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0003.jpg)
![Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (SPM150) [NBP2-32999] - Analysis of human lung carcinoma using TTF-1 / NKX2-1 antibody. Incubated over night in 4C with the primary antibody, 1.0ug/ml. Image from verified customer review.](http://images.novusbio.com/fullsize/TTF-1-NKX2-1-Antibody-SPM150-Immunohistochemistry-Paraffin-NBP2-32999-img0002.jpg "Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (SPM150) [NBP2-32999] - Analysis of human lung carcinoma using TTF-1 / NKX2-1 antibody. Incubated over night in 4C with the primary antibody, 1.0ug/ml. Image from verified customer review.")
![Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (SPM150) [NBP2-32999] - Human LUng Carcinoma](http://images.novusbio.com/fullsize/TTF-1-NKX2-1-Antibody-SPM150-Immunohistochemistry-Paraffin-NBP2-32999-img0001.jpg)
![Western Blot: FKBP52/FKBP4 Antibody (5C11) [H00002288-M01] - FKBP4 monoclonal antibody (M01), clone 5C11. Analysis of FKBP4 expression in NIH/3T3.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-5C11-Western-Blot-H00002288-M01-img0014.jpg "Western Blot: FKBP52/FKBP4 Antibody (5C11) [H00002288-M01] - FKBP4 monoclonal antibody (M01), clone 5C11. Analysis of FKBP4 expression in NIH/3T3.")
![Immunohistochemistry-Paraffin: FKBP52/FKBP4 Antibody (5C11) [H00002288-M01] - Analysis of monoclonal antibody to FKBP4 on formalin-fixed paraffin-embedded human uterine cervix. Antibody concentration 0.5 ug/ml.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-5C11-Immunohistochemistry-Paraffin-H00002288-M01-img0008.jpg)
![Western Blot: FKBP52/FKBP4 Antibody (5C11) [H00002288-M01] - FKBP4 monoclonal antibody (M01), clone 5C11 Analysis of FKBP4 expression in HeLa.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-5C11-Western-Blot-H00002288-M01-img0011.jpg)
![Western Blot: FKBP52/FKBP4 Antibody (5C11) [H00002288-M01] - FKBP4 monoclonal antibody (M01), clone 5C11. Analysis of FKBP4 expression in PC-12.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-5C11-Western-Blot-H00002288-M01-img0012.jpg)
![Western Blot: FKBP52/FKBP4 Antibody (5C11) [H00002288-M01] - FKBP4 monoclonal antibody (M01), clone 5C11. Analysis of FKBP4 expression in Raw 264.7.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-5C11-Western-Blot-H00002288-M01-img0013.jpg)
![Immunoprecipitation: FKBP52/FKBP4 Antibody (5C11) [H00002288-M01] - Analysis of FKBP4 transfected lysate using anti-FKBP4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FKBP4 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-5C11-Immunoprecipitation-H00002288-M01-img0009.jpg)
![ELISA: FKBP52/FKBP4 Antibody (5C11) [H00002288-M01] - Detection limit for recombinant GST tagged FKBP4 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-5C11-ELISA-H00002288-M01-img0010.jpg)