| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1202AF405 | |||
| Alexa Fluor 488 | NB21-1202AF488 | |||
| Alexa Fluor 647 | NB21-1202AF647 | |||
| Alexa Fluor 700 | NB21-1202AF700 | |||
| Biotin | NB21-1202B | |||
| DyLight 405 | NB21-1202V | |||
| DyLight 488 | NB21-1202G | |||
| DyLight 550 | NB21-1202R | |||
| DyLight 650 | NB21-1202C | |||
| FITC | NB21-1202F | |||
| HRP | NB21-1202H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1202SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1052AF405 | |||
| Alexa Fluor 488 | NB21-1052AF488 | |||
| Alexa Fluor 647 | NB21-1052AF647 | |||
| Alexa Fluor 700 | NB21-1052AF700 | |||
| Biotin | NB21-1052B | |||
| DyLight 405 | NB21-1052V | |||
| DyLight 488 | NB21-1052G | |||
| DyLight 550 | NB21-1052R | |||
| DyLight 650 | NB21-1052C | |||
| FITC | NB21-1052F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1052SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1132AF405 | |||
| Alexa Fluor 488 | NB21-1132AF488 | |||
| Alexa Fluor 647 | NB21-1132AF647 | |||
| Alexa Fluor 700 | NB21-1132AF700 | |||
| Biotin | NB21-1132B | |||
| DyLight 405 | NB21-1132V | |||
| DyLight 488 | NB21-1132G | |||
| DyLight 550 | NB21-1132R | |||
| DyLight 650 | NB21-1132C | |||
| FITC | NB21-1132F | |||
| HRP | NB21-1132H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1132SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1188AF405 | |||
| Alexa Fluor 488 | NB21-1188AF488 | |||
| Alexa Fluor 647 | NB21-1188AF647 | |||
| Alexa Fluor 700 | NB21-1188AF700 | |||
| Biotin | NB21-1188B | |||
| DyLight 405 | NB21-1188V | |||
| DyLight 488 | NB21-1188G | |||
| DyLight 550 | NB21-1188R | |||
| DyLight 650 | NB21-1188C | |||
| FITC | NB21-1188F | |||
| HRP | NB21-1188H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1188SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1142AF405 | |||
| Alexa Fluor 488 | NB21-1142AF488 | |||
| Alexa Fluor 647 | NB21-1142AF647 | |||
| Alexa Fluor 700 | NB21-1142AF700 | |||
| Biotin | NB21-1142B | |||
| DyLight 405 | NB21-1142V | |||
| DyLight 488 | NB21-1142G | |||
| DyLight 550 | NB21-1142R | |||
| DyLight 650 | NB21-1142C | |||
| FITC | NB21-1142F | |||
| HRP | NB21-1142H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1142SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37718 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1144AF405 | |||
| Alexa Fluor 488 | NB21-1144AF488 | |||
| Alexa Fluor 647 | NB21-1144AF647 | |||
| Alexa Fluor 700 | NB21-1144AF700 | |||
| Biotin | NB21-1144B | |||
| DyLight 405 | NB21-1144V | |||
| DyLight 488 | NB21-1144G | |||
| DyLight 550 | NB21-1144R | |||
| DyLight 650 | NB21-1144C | |||
| FITC | NB21-1144F | |||
| HRP | NB21-1144H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1144SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB93441-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1252AF405 | |||
| Alexa Fluor 488 | NB21-1252AF488 | |||
| Alexa Fluor 647 | NB21-1252AF647 | |||
| Alexa Fluor 700 | NB21-1252AF700 | |||
| Biotin | NB21-1252B | |||
| DyLight 405 | NB21-1252V | |||
| DyLight 488 | NB21-1252G | |||
| DyLight 550 | NB21-1252R | |||
| DyLight 650 | NB21-1252C | |||
| FITC | NB21-1252F | |||
| HRP | NB21-1252H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1252SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1039AF405 | |||
| Alexa Fluor 488 | NB21-1039AF488 | |||
| Alexa Fluor 647 | NB21-1039AF647 | |||
| Alexa Fluor 700 | NB21-1039AF700 | |||
| Biotin | NB21-1039B | |||
| DyLight 405 | NB21-1039V | |||
| DyLight 488 | NB21-1039G | |||
| DyLight 550 | NB21-1039R | |||
| DyLight 650 | NB21-1039C | |||
| FITC | NB21-1039F | |||
| HRP | NB21-1039H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1039SS | |||
![Western Blot: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Analysis of Histone H3 R2me2s/K4Me2 in C. elegans embryo lysate. Observed molecular weight is ~13 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--Sym-dimethyl-Arg2]-Antibody-Western-Blot-NB21-1202-img0012.jpg "Western Blot: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Analysis of Histone H3 R2me2s/K4Me2 in C. elegans embryo lysate. Observed molecular weight is ~13 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1202 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--Sym-dimethyl-Arg2]-Antibody-Chromatin-Immunoprecipitation-NB21-1202-img0013.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Analysis of H3R2me2s/K4Me2 in picomoles of peptide. Lanes (1) R2Me2 only, (2) Both Modifications, and (3) K4Me2.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--Sym-dimethyl-Arg2]-Antibody-Dot-Blot-NB21-1202-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Histone H3.2 R2me2s/K4me2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 594 (red).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--Sym-dimethyl-Arg2]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1202-img0016.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Analysis of Histone R2 in NIH 3T3 histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--Sym-dimethyl-Arg2]-Antibody-Western-Blot-NB21-1202-img0011.jpg)
![Dot Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Analysis of H3K9me1/pT6 in picomoles of protein.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Dot-Blot-NB21-1052-img0011.jpg "Dot Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Analysis of H3K9me1/pT6 in picomoles of protein.")
![Chromatin Immunoprecipitation: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of NB21-1052 and 20 uL of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1052-img0010.jpg)
![Western Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Analysis of H3K9me1/pT6 in HeLa Histone prep lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Western-Blot-NB21-1052-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1052-img0017.jpg)
![Western Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Analysis of H3K9me1/pT6 in NIH 3T3 Histone prep lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Western-Blot-NB21-1052-img0014.jpg)
![Western Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Analysis of H3K9me1/pT6 in C. elegans embryo lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Western-Blot-NB21-1052-img0012.jpg)
![Western Blot: Histone H3 [Asym-dimethyl Arg17] Antibody [NB21-1132] - Analysis of HIstone H3 R17me2a in NIH 3T3 histone prep cell lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg17]-Antibody-Western-Blot-NB21-1132-img0011.jpg "Western Blot: Histone H3 [Asym-dimethyl Arg17] Antibody [NB21-1132] - Analysis of HIstone H3 R17me2a in NIH 3T3 histone prep cell lysate. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Asym-dimethyl Arg17] Antibody [NB21-1132] - 2 ug of NB21-1132 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg17]-Antibody-Chromatin-Immunoprecipitation-NB21-1132-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Asym-dimethyl Arg17] Antibody [NB21-1132] - Histone H3.2 R17me2a antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg17]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1132-img0013.jpg)
![Western Blot: Histone H3 [Asym-dimethyl Arg17] Antibody [NB21-1132] - Analysis of Histone H3 R17me2a in C. elegans embryo lysate. Observed molecular weight is ~10 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg17]-Antibody-Western-Blot-NB21-1132-img0010.jpg)
![Dot Blot: Histone H3 [p Ser28] Antibody [NB21-1188] - Dot blot analysis of Histone H3 pS28 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28]-Antibody-Dot-Blot-NB21-1188-img0003.jpg "Dot Blot: Histone H3 [p Ser28] Antibody [NB21-1188] - Dot blot analysis of Histone H3 pS28 in picomoles of peptide.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser28] Antibody [NB21-1188] - Histone H3.2 pS28 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1188-img0007.jpg)
![Western Blot: Histone H3 [p Ser28] Antibody [NB21-1188] - WB analysis of Histone H3 pS28 in NIH 3T3 histone prep. Observed molecular weight is ~14 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28]-Antibody-Western-Blot-NB21-1188-img0002.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - Analysis of H3K18me2 antibody using peptides stated above in 1.0, 0.3 and 0.1 picomoles.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys18]-Antibody-Dot-Blot-NB21-1142-img0011.jpg "Dot Blot: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - Analysis of H3K18me2 antibody using peptides stated above in 1.0, 0.3 and 0.1 picomoles.")
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 ug of NB21-1142 and 20 uL of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys18]-Antibody-Chromatin-Immunoprecipitation-NB21-1142-img0010.jpg)
![Western Blot: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - Analysis of H3.2 K18me2 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys18]-Antibody-Western-Blot-NB21-1142-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - Antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys18]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1142-img0016.jpg)
![Western Blot: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - Detection of Histone H3 [dimethyl Lys18] in NIH 3T3 histone preps. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys18]-Antibody-Western-Blot-NB21-1142-img0012.jpg)
![Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates with Rabbit anti-Lamin B1 antibody (10 ug/Lane Lane 2: PC-12 cell lysate, 10 ug/Lane Lane 3: Mouse liver tissue lysate, 20 ug/Lane Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Western-Blot-NBP2-67405-img0008.jpg "Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates with Rabbit anti-Lamin B1 antibody (10 ug/Lane Lane 2: PC-12 cell lysate, 10 ug/Lane Lane 3: Mouse liver tissue lysate, 20 ug/Lane Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of HepG2 cells labeling Lamin B1 with Rabbit anti-Lamin B1 antibody permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lamin B1 antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunocytochemistry-Immunofluorescence-NBP2-67405-img0009.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0012.jpg)
![Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysateLane 2: Jurkat cell lysate](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Western-Blot-NBP2-67405-img0006.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0001.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human colon cancer tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0002.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human liver tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded mouse colon tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0005.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Lamin B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0010.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0011.jpg)
![Western Blot: Transferrin Antibody (HTF-14) [NB500-418] - Analysis using the Azide Free version of NB500-418. Detection of 1. hTransferrin; 5 ug/well (red. con.) 2. hTransferrin; 3 ug/well (red. con.) 3. hTransferrin; 1 ug/well (red. con.) M. Low Range marker 4. hTransferrin; 1 ug/well (non-red. con.) 5. hTransferrin; 3 ug/well (non-red. con.) 6. hTransferrin; 5 ug/well (non-red. con.)](http://images.novusbio.com/fullsize/Transferrin-Antibody-HTF-14-Western-Blot-NB500-418-img0002.jpg "Western Blot: Transferrin Antibody (HTF-14) [NB500-418] - Analysis using the Azide Free version of NB500-418. Detection of 1. hTransferrin; 5 ug/well (red. con.) 2. hTransferrin; 3 ug/well (red. con.) 3. hTransferrin; 1 ug/well (red. con.) M. Low Range marker 4. hTransferrin; 1 ug/well (non-red. con.) 5. hTransferrin; 3 ug/well (non-red. con.) 6. hTransferrin; 5 ug/well (non-red. con.)")
![Immunohistochemistry-Paraffin: Transferrin Antibody (HTF-14) [NB500-418] - Staining of human placenta (paraffin sections) using anti-transferrin (HTF-14).](http://images.novusbio.com/fullsize/Transferrin-Antibody-HTF-14-Immunohistochemistry-Paraffin-NB500-418-img0003.jpg)
![Dot Blot: Histone H3 [ac Lys18] Antibody [NB21-1144] - Analysis of Histone H3 K18ac in the peptides listed above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Dot-Blot-NB21-1144-img0005.jpg "Dot Blot: Histone H3 [ac Lys18] Antibody [NB21-1144] - Analysis of Histone H3 K18ac in the peptides listed above in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys18] Antibody [NB21-1144] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1144 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Chromatin-Immunoprecipitation-NB21-1144-img0010.jpg)
![Western Blot: Histone H3 [ac Lys18] Antibody [NB21-1144] - Analysis of Histone H3 K18ac in (1) HeLa histone prep, (2) NIH 3T3 histone prep and (3) C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Western-Blot-NB21-1144-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys18] Antibody [NB21-1144] - Histone H3 [ac Lys18] antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1144-img0009.jpg)
![Western Blot: alpha Tubulin Antibody (961258) [MAB93441] - Detection of Human, Mouse, and Rat alpha Tubulin by Western Blot. Western blot shows lysates of HeLa, Jurkat, NIH-3T3, Neuro2A and Rat-2 cell lines. PVDF membrane was probed with Mouse alpha Tubulin Antibody (961258) (Catalog # MAB93441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha Tubulin at an approximate molecular weight of 50 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-961258-Western-Blot-MAB93441-img0004.jpg "Western Blot: alpha Tubulin Antibody (961258) [MAB93441] - Detection of Human, Mouse, and Rat alpha Tubulin by Western Blot. Western blot shows lysates of HeLa, Jurkat, NIH-3T3, Neuro2A and Rat-2 cell lines. PVDF membrane was probed with Mouse alpha Tubulin Antibody (961258) (Catalog # MAB93441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha Tubulin at an approximate molecular weight of 50 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody (961258) [MAB93441] - alpha Tubulin was detected in immersion fixed paraffin-embedded sections of human brin hippocampus tissue using alpha Tubulin Antibody (961258) at 5 ug/ml. Tissue was stained using VC001 (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm in cancer cells.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-961258-Immunohistochemistry-Paraffin-MAB93441-img0002.jpg)
![Immunoprecipitation: Histone H3 [ac Lys27] Antibody-Histone H3 [ac Lys27] Antibody (2V10N10)-[NBP3-15933] -](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-immunoprecipitation-622024101825..jpg "Immunoprecipitation: Histone H3 [ac Lys27] Antibody-Histone H3 [ac Lys27] Antibody (2V10N10)-[NBP3-15933] -")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody (2V10N10)- [NBP3-15933] - Analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-chromatin-immunoprecipitation-622024102155..jpg)
![Dot Blot-Histone H3 [ac Lys27] Antibody (2V10N10)-NBP3-15933- Analysis of all sorts of peptides using Acetyl-Histone H3-K27 Rabbit mAb at 1:200000 dilution.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-dot-blot-622024102348..jpg)
![<b>Biological Strategies Validation. </b>Western Blot: -Histone H3 [ac Lys27] Antibody (2V10N10)-NBP3-15933-analysis of lysates from C2C12 cells using Acetyl-Histone H3-K27 Rabbit mAb at 1:100000 dilution. C2C12 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 20 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-western-blot-622024102550..jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517213518.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-5220251726021.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517192955.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517284941.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunofluorescence analysis of HeLa treated with TSA and HeLa cells using Histone H3 Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517284953.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517202962.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517223970.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517273516.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-5220251723576.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Analysis of H3 K36-Me2 using the peptides stated above in 10, 6, 3 and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Dot-Blot-NB21-1252-img0010.jpg "Dot Blot: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Analysis of H3 K36-Me2 using the peptides stated above in 10, 6, 3 and 1 picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 ug of NB21-1252 and 20 uL of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-NB21-1252-img0009.jpg)
![Western Blot: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Analysis of H3 K36Me2 in C. elegans embryo lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Western-Blot-NB21-1252-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Antibody was tested in Neuro2a cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1252-img0016.jpg)
![Western Blot: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Analysis of H3K36me2 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Western-Blot-NB21-1252-img0011.jpg)
![Dot Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - Dot blot analysis of H3K4me3/pT6 with the peptides stated above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Dot-Blot-NB21-1039-img0010.jpg "Dot Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - Dot blot analysis of H3K4me3/pT6 with the peptides stated above in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - 2 ug of NB21-1039 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control, DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1039-img0014.jpg)
![Western Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - WB analysis of H3K4Me3/pT6 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Western-Blot-NB21-1039-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - Histone H3.2 K4me3 pT6 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1039-img0017.jpg)
![Western Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - WB detection of H3K4me3/pT6 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Western-Blot-NB21-1039-img0011.jpg)