| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85358-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1021AF405 | |||
| Alexa Fluor 488 | NB21-1021AF488 | |||
| Alexa Fluor 647 | NB21-1021AF647 | |||
| Alexa Fluor 700 | NB21-1021AF700 | |||
| Biotin | NB21-1021B | |||
| DyLight 405 | NB21-1021V | |||
| DyLight 488 | NB21-1021G | |||
| DyLight 550 | NB21-1021R | |||
| DyLight 650 | NB21-1021C | |||
| FITC | NB21-1021F | |||
| HRP | NB21-1021H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1021SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1002AF405 | |||
| Alexa Fluor 488 | NB21-1002AF488 | |||
| Alexa Fluor 647 | NB21-1002AF647 | |||
| Alexa Fluor 700 | NB21-1002AF700 | |||
| Biotin | NB21-1002B | |||
| DyLight 405 | NB21-1002V | |||
| DyLight 488 | NB21-1002G | |||
| DyLight 550 | NB21-1002R | |||
| DyLight 650 | NB21-1002C | |||
| FITC | NB21-1002F | |||
| HRP | NB21-1002H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1002SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37828-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1081AF405 | |||
| Alexa Fluor 488 | NB21-1081AF488 | |||
| Alexa Fluor 647 | NB21-1081AF647 | |||
| Alexa Fluor 700 | NB21-1081AF700 | |||
| Biotin | NB21-1081B | |||
| DyLight 405 | NB21-1081V | |||
| DyLight 488 | NB21-1081G | |||
| DyLight 550 | NB21-1081R | |||
| DyLight 650 | NB21-1081C | |||
| FITC | NB21-1081F | |||
| HRP | NB21-1081H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1081SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1072AF405 | |||
| Alexa Fluor 488 | NB21-1072AF488 | |||
| Alexa Fluor 647 | NB21-1072AF647 | |||
| Alexa Fluor 700 | NB21-1072AF700 | |||
| Biotin | NB21-1072B | |||
| DyLight 405 | NB21-1072V | |||
| DyLight 488 | NB21-1072G | |||
| DyLight 550 | NB21-1072R | |||
| DyLight 650 | NB21-1072C | |||
| FITC | NB21-1072F | |||
| HRP | NB21-1072H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1072SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-267SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-1383V | |||
| FITC | NB21-1383F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1383SS | |||
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining in human thyroid gland and skeletal muscle tissues using anti-PPIB antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining in human thyroid gland and skeletal muscle tissues using anti-PPIB antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Cyclophilin B Antibody [NBP1-85358] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP1-85358-img0009.jpg)
![Western Blot: Cyclophilin B Antibody [NBP1-85358] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP1-85358-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclophilin B Antibody [NBP1-85358] - Staining of human cell line HUVEC TERT2 shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85358-img0007.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0017.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0010.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human thyroid gland shows high expression.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0011.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human prostate shows weak granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0015.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human endometrium shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0016.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - Analysis of Histone H3K4me1 in HeLa histone prep lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Antibody-Western-Blot-NB21-1021-img0002.jpg "Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - Analysis of Histone H3K4me1 in HeLa histone prep lysate. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - Histone H3.2 K4me1 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Antibody-Immunocytochemistry-NB21-1021-img0001.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - H3K4Me1 immunoblot in Jurkat cells. Observed molecular weight is ~17 kDa. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Antibody-Western-Blot-NB21-1021-img0006.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - Analysis of H3K4me1 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Antibody-Western-Blot-NB21-1021-img0003.jpg)
![Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Western-Blot-H00026330-M01-img0009.jpg "Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of monoclonal antibody to GAPDHS on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Immunohistochemistry-Paraffin-H00026330-M01-img0007.jpg)
![Sandwich ELISA: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Detection limit for recombinant GST tagged GAPDHS is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Sandwich-ELISA-H00026330-M01-img0008.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-326 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0005.jpg "Western Blot: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-326 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-326). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-326-img0004.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of Mouse GAPDH Whole cell lysate (5, 15 and 50 ug) from mouse NIH3T3 cells. Affinity purified rabbit anti-GAPDH antibody used at 0.04 ug/ml. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0002.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of GAPDH in nu/nu Mouse cells. 5% BSA in PBS, 1:5,000. Secondary: Goat anti-Rabbit IgG 1:5,000. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0003.jpg)
![Simple Western: GAPDH Antibody [NB300-326] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-326-img0001.jpg)
![Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0046.jpg "<b>Biological Strategies Validation. </b>Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PCNA Antibody [NB100-456] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- PCNA used at a dilution of 1:10,000 (0.1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-NB100-456-img0044.jpg)
![Flow Cytometry: PCNA Antibody [NB100-456] - Detection of PCNA Versus DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% triton-X-100, 0.5mM EDTA, 1% BSA) and 100% methanol. 1 X106 cells were stained with 0.03 ug anti-PCNA . Secondary detection was performed with FITC conjugated Goat F(ab')2 anti-rabbit antibody], and DNA stained with PI.](http://images.novusbio.com/fullsize/PCNA-Antibody-Flow-Cytometry-NB100-456-img0004.jpg)
![Western Blot: PCNA Antibody [NB100-456] - A431 whole cell lysates](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0041.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PCNA Antibody [NB100-456] - Analysis using the Biotin conjugate of NB100-456. Detection ofHeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0043.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PCNA Antibody [NB100-456] - Detection of human PCNA by western blot and immunoprecipitation. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 ug for IP; 30 ug for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody NB100-456 used at the indicated concentrations for WB. B. PCNA was immunoprecipitated using another a mouse monoclonal antibody to PCNA (mAb). Control mock IP was performed using normal mouse IgG (M-IgG) and normal rabbit IgG (R-IgG). immunoprecipitatesd PCNA was blotted using NB100-456 (1 ug/ml). Detection: Chemiluminescence with an exposure time of 2 minutes (A) or 5 seconds (B).](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunoprecipitation-NB100-456-img0047.jpg)
![Dot Blot: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Analysis of Histone H3 [Asym-dimethyl Arg2] in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg2]-Antibody-Dot-Blot-NB21-1002-img0007.jpg "Dot Blot: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Analysis of Histone H3 [Asym-dimethyl Arg2] in picomoles of peptide.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Histone H3 [Asym-dimethyl Arg2] antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg2]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1002-img0006.jpg)
![Western Blot: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Analysis of Histone H3 [Asym-dimethyl Arg2] in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg2]-Antibody-Western-Blot-NB21-1002-img0008.jpg)
![Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3). Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Western-Blot-NBP2-37828-img0019.jpg "Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3). Theoretical molecular weight: 36 kDa.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) in HeLa. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a GAPDH monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunocytochemistry-Immunofluorescence-NBP2-37828-img0012.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of paraffin-embedded human breast carcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunohistochemistry-Paraffin-NBP2-37828-img0015.jpg)
![Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in Hela cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Flow-Cytometry-NBP2-37828-img0008.jpg)
![Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of 5ug/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Western-Blot-NBP2-37828-img0018.jpg)
![Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of 20ug of THP-1 whole cell lysates per well onto an SDS-PAGE gel. GAPDH theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Western-Blot-NBP2-37828-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) showing staining in the in the cytoplasm of MCF-7 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunocytochemistry-Immunofluorescence-NBP2-37828-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) showing staining in the in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunocytochemistry-Immunofluorescence-NBP2-37828-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunocytochemistry-Immunofluorescence-NBP2-37828-img0011.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of mouse colon tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunohistochemistry-Paraffin-NBP2-37828-img0013.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of human kidney tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunohistochemistry-Paraffin-NBP2-37828-img0014.jpg)
![Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in NIH-3T3 cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Flow-Cytometry-NBP2-37828-img0006.jpg)
![Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in MCF-7 cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Flow-Cytometry-NBP2-37828-img0007.jpg)
![Dot Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - Dot blot analysis of H3 K36ac in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Dot-Blot-NB21-1081-img0008.jpg "Dot Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - Dot blot analysis of H3 K36ac in picomoles of peptide.")
![Western Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - WB analysis of H3K9ac/K14ac in C. elegans embryo lysate. Observed molecular weight is ~11 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Western-Blot-NB21-1081-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - Histone H3.2 K9,K14ac antibody was tested at 1:50 in HeLa cells with FITC. Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Immunocytochemistry-NB21-1081-img0012.jpg)
![Flow Cytometry: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - Analysis of Alexa Fluor (R) 700 conjugate of NB21-1081. Jurkat cells stained with Alexa Fluor 700 conjugated acetylated Histone H3 antibody (blue) or isotype control (red). Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Flow-Cytometry-NB21-1081-img0013.jpg)
![Western Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - WB analysis of H3 K9ac/K14ac in NIH/3T3 histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Western-Blot-NB21-1081-img0009.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Analysis of H3K9me2 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Dot-Blot-NB21-1072-img0010.jpg "Dot Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Analysis of H3K9me2 in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1072 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1072-img0009.jpg)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Analysis of Histone H3K9me2 on HeLa histone preps. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Western-Blot-NB21-1072-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Histone H3.2 K9me2 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1072-img0008.jpg)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Analysis of H3K9me2 in C. elegans embryo lysate. Observed molecular weight is ~13 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Western-Blot-NB21-1072-img0012.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-59206] - Western blot was performed on whole cell (40 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Western-Blot-NBP2-59206-img0006.jpg "Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-59206] - Western blot was performed on whole cell (40 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-59206] - HeLa cells were stained with the antibody against H3K27me3 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K27me3 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59206-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys27] Antibody [NBP2-59206] - ChIP assays were performed using human K562 cells, the antibody against H3K27me3 and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 100,000 cells. The indicated amounts of antibody were used per ChIP experiment. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as negative controls, and TSH2B and MYT1, used as positive controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Chromatin-Immunoprecipitation-NBP2-59206-img0003.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-59206] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27me3 diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Western-Blot-NBP2-59206-img0001.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys27] Antibody [NBP2-59206] - ChIP assays were performed using human K562 cells, the antibody against H3K27me3 and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. The indicated amounts of antibody were used per ChIP experiment. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as negative controls, and TSH2B and MYT1, used as positive controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Chromatin-Immunoprecipitation-NBP2-59206-img0002.jpg)
![ELISA: Histone H3 [Trimethyl Lys27] Antibody [NBP2-59206] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K27me3. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:1,000,000.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-ELISA-NBP2-59206-img0004.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-59206] - To test the cross reactivity of the antibody against H3K27me3, a Dot Blot analysis was performed with peptides containing other modifications or unmodified sequences of histone H3 and H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Dot-Blot-NBP2-59206-img0005.jpg)
![Western Blot: Histone H3 Antibody [NB500-267] - Detection of Histone H3 in lysates using NB500-267. (1) MEF and (2) HeLa. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-267-img0003.jpg "Western Blot: Histone H3 Antibody [NB500-267] - Detection of Histone H3 in lysates using NB500-267. (1) MEF and (2) HeLa. Observed molecular weight is ~15 kDa.")
![Western Blot: Histone H3 Antibody [NB500-267] - Analysis of HeLa lysates using NB500-267. Image courtesy of Gregg Semenza, (PMID: 21620138). Theoretical molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-267-img0004.jpg)
![Western Blot: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Analysis of H3 K79me3 in (1) HeLa Histone prep (2) 3T3 Histone prep, and (3) C.elegans embryo lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Western-Blot-NB21-1383-img0006.jpg "Western Blot: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Analysis of H3 K79me3 in (1) HeLa Histone prep (2) 3T3 Histone prep, and (3) C.elegans embryo lysate. Observed molecular weight is ~16 kDa.")
![Dot Blot: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Analysis of H3 K79me3 using the peptides listed above in 100, 10, and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Dot-Blot-NB21-1383-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-1383 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Chromatin-Immunoprecipitation-NB21-1383-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Immunofluorescence: [NB21-1383] - H3 K79me3 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Immunocytochemistry-NB21-1383-img0009.jpg)