| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1024AF405 | |||
| Alexa Fluor 488 | NB21-1024AF488 | |||
| Alexa Fluor 647 | NB21-1024AF647 | |||
| Alexa Fluor 700 | NB21-1024AF700 | |||
| Biotin | NB21-1024B | |||
| DyLight 405 | NB21-1024V | |||
| DyLight 488 | NB21-1024G | |||
| DyLight 550 | NB21-1024R | |||
| DyLight 650 | NB21-1024C | |||
| FITC | NB21-1024F | |||
| HRP | NB21-1024H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1024SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-1383V | |||
| FITC | NB21-1383F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1383SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1052AF405 | |||
| Alexa Fluor 488 | NB21-1052AF488 | |||
| Alexa Fluor 647 | NB21-1052AF647 | |||
| Alexa Fluor 700 | NB21-1052AF700 | |||
| Biotin | NB21-1052B | |||
| DyLight 405 | NB21-1052V | |||
| DyLight 488 | NB21-1052G | |||
| DyLight 550 | NB21-1052R | |||
| DyLight 650 | NB21-1052C | |||
| FITC | NB21-1052F | |||
| HRP | NB21-1052H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1052SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1202AF405 | |||
| Alexa Fluor 488 | NB21-1202AF488 | |||
| Alexa Fluor 647 | NB21-1202AF647 | |||
| Alexa Fluor 700 | NB21-1202AF700 | |||
| Biotin | NB21-1202B | |||
| DyLight 405 | NB21-1202V | |||
| DyLight 488 | NB21-1202G | |||
| DyLight 550 | NB21-1202R | |||
| DyLight 650 | NB21-1202C | |||
| FITC | NB21-1202F | |||
| HRP | NB21-1202H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1202SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1132AF405 | |||
| Alexa Fluor 488 | NB21-1132AF488 | |||
| Alexa Fluor 647 | NB21-1132AF647 | |||
| Alexa Fluor 700 | NB21-1132AF700 | |||
| Biotin | NB21-1132B | |||
| DyLight 405 | NB21-1132V | |||
| DyLight 488 | NB21-1132G | |||
| DyLight 550 | NB21-1132R | |||
| DyLight 650 | NB21-1132C | |||
| FITC | NB21-1132F | |||
| HRP | NB21-1132H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1132SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1039AF405 | |||
| Alexa Fluor 488 | NB21-1039AF488 | |||
| Alexa Fluor 647 | NB21-1039AF647 | |||
| Alexa Fluor 700 | NB21-1039AF700 | |||
| Biotin | NB21-1039B | |||
| DyLight 405 | NB21-1039V | |||
| DyLight 488 | NB21-1039G | |||
| DyLight 550 | NB21-1039R | |||
| DyLight 650 | NB21-1039C | |||
| FITC | NB21-1039F | |||
| HRP | NB21-1039H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1039SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89503-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1071AF405 | |||
| Alexa Fluor 488 | NB21-1071AF488 | |||
| Alexa Fluor 647 | NB21-1071AF647 | |||
| Alexa Fluor 700 | NB21-1071AF700 | |||
| Biotin | NB21-1071B | |||
| DyLight 405 | NB21-1071V | |||
| DyLight 488 | NB21-1071G | |||
| DyLight 550 | NB21-1071R | |||
| DyLight 650 | NB21-1071C | |||
| FITC | NB21-1071F | |||
| HRP | NB21-1071H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1071SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1252AF405 | |||
| Alexa Fluor 488 | NB21-1252AF488 | |||
| Alexa Fluor 647 | NB21-1252AF647 | |||
| Alexa Fluor 700 | NB21-1252AF700 | |||
| Biotin | NB21-1252B | |||
| DyLight 405 | NB21-1252V | |||
| DyLight 488 | NB21-1252G | |||
| DyLight 550 | NB21-1252R | |||
| DyLight 650 | NB21-1252C | |||
| FITC | NB21-1252F | |||
| HRP | NB21-1252H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1252SS | |||
![Dot Blot: Histone H3 [ac Lys4] Antibody [NB21-1024] - Analysis of Histone H3 K4ac antibody in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Dot-Blot-NB21-1024-img0015.jpg "Dot Blot: Histone H3 [ac Lys4] Antibody [NB21-1024] - Analysis of Histone H3 K4ac antibody in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys4] Antibody [NB21-1024] - Histone H3 [ac Lys4] Antibody [NB21-1024] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1024 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1024-img0019.jpg)
![Western Blot: Histone H3 [ac Lys4] Antibody [NB21-1024] - Analysis of Histone H3 K4ac in C. elegans embryo lysate. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Western-Blot-NB21-1024-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys4] Antibody [NB21-1024] - Histone H3 K4ac1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1024-img0021.jpg)
![Western Blot: Histone H3 [ac Lys4] Antibody [NB21-1024] - Analysis of Histone H3 K4ac in (1) HeLa histone prep and (2) NIH 3T3 histone prep lysates. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Western-Blot-NB21-1024-img0016.jpg)
![Western Blot: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Analysis of H3 K79me3 in (1) HeLa Histone prep (2) 3T3 Histone prep, and (3) C.elegans embryo lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Western-Blot-NB21-1383-img0006.jpg "Western Blot: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Analysis of H3 K79me3 in (1) HeLa Histone prep (2) 3T3 Histone prep, and (3) C.elegans embryo lysate. Observed molecular weight is ~16 kDa.")
![Dot Blot: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Analysis of H3 K79me3 using the peptides listed above in 100, 10, and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Dot-Blot-NB21-1383-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-1383 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Chromatin-Immunoprecipitation-NB21-1383-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Immunofluorescence: [NB21-1383] - H3 K79me3 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Immunocytochemistry-NB21-1383-img0009.jpg)
![Dot Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Analysis of H3K9me1/pT6 in picomoles of protein.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Dot-Blot-NB21-1052-img0011.jpg "Dot Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Analysis of H3K9me1/pT6 in picomoles of protein.")
![Chromatin Immunoprecipitation: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of NB21-1052 and 20 uL of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1052-img0010.jpg)
![Western Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Analysis of H3K9me1/pT6 in HeLa Histone prep lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Western-Blot-NB21-1052-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1052-img0017.jpg)
![Western Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Analysis of H3K9me1/pT6 in NIH 3T3 Histone prep lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Western-Blot-NB21-1052-img0014.jpg)
![Western Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Analysis of H3K9me1/pT6 in C. elegans embryo lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Monomethyl-Lys9]-Antibody-Western-Blot-NB21-1052-img0012.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Analysis of Histone H3 R2me2s/K4Me2 in C. elegans embryo lysate. Observed molecular weight is ~13 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--Sym-dimethyl-Arg2]-Antibody-Western-Blot-NB21-1202-img0012.jpg "Western Blot: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Analysis of Histone H3 R2me2s/K4Me2 in C. elegans embryo lysate. Observed molecular weight is ~13 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1202 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--Sym-dimethyl-Arg2]-Antibody-Chromatin-Immunoprecipitation-NB21-1202-img0013.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Analysis of H3R2me2s/K4Me2 in picomoles of peptide. Lanes (1) R2Me2 only, (2) Both Modifications, and (3) K4Me2.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--Sym-dimethyl-Arg2]-Antibody-Dot-Blot-NB21-1202-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Histone H3.2 R2me2s/K4me2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 594 (red).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--Sym-dimethyl-Arg2]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1202-img0016.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Analysis of Histone R2 in NIH 3T3 histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--Sym-dimethyl-Arg2]-Antibody-Western-Blot-NB21-1202-img0011.jpg)
![Western Blot: Histone H3 [Asym-dimethyl Arg17] Antibody [NB21-1132] - Analysis of HIstone H3 R17me2a in NIH 3T3 histone prep cell lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg17]-Antibody-Western-Blot-NB21-1132-img0011.jpg "Western Blot: Histone H3 [Asym-dimethyl Arg17] Antibody [NB21-1132] - Analysis of HIstone H3 R17me2a in NIH 3T3 histone prep cell lysate. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Asym-dimethyl Arg17] Antibody [NB21-1132] - 2 ug of NB21-1132 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg17]-Antibody-Chromatin-Immunoprecipitation-NB21-1132-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Asym-dimethyl Arg17] Antibody [NB21-1132] - Histone H3.2 R17me2a antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg17]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1132-img0013.jpg)
![Western Blot: Histone H3 [Asym-dimethyl Arg17] Antibody [NB21-1132] - Analysis of Histone H3 R17me2a in C. elegans embryo lysate. Observed molecular weight is ~10 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg17]-Antibody-Western-Blot-NB21-1132-img0010.jpg)
![Dot Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - Dot blot analysis of H3K4me3/pT6 with the peptides stated above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Dot-Blot-NB21-1039-img0010.jpg "Dot Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - Dot blot analysis of H3K4me3/pT6 with the peptides stated above in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - 2 ug of NB21-1039 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control, DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1039-img0014.jpg)
![Western Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - WB analysis of H3K4Me3/pT6 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Western-Blot-NB21-1039-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - Histone H3.2 K4me3 pT6 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1039-img0017.jpg)
![Western Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - WB detection of H3K4me3/pT6 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Western-Blot-NB21-1039-img0011.jpg)
![Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06859] - Simple Western performed with monoclonal GAPDH antibody, MAB5718, on Jurkat (human acute T cell leukemia), HeLa (human cervical cancer cell line), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1ug/ml dilution.](http://images.novusbio.com/fullsize/Loading-Control-Simple-Western-Antibody-Pack-Simple-Western-NBP3-06859-img0001.jpg "Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06859] - Simple Western performed with monoclonal GAPDH antibody, MAB5718, on Jurkat (human acute T cell leukemia), HeLa (human cervical cancer cell line), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1ug/ml dilution.")
![Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06859] - Simple Western performed with monoclonal beta-Actin antibody, MAB8929, on Jurkat (human acute T cell leukemia), HeLa (human cervical cancer cell line), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1ug/ml dilution.](http://images.novusbio.com/fullsize/Loading-Control-Simple-Western-Antibody-Pack-Simple-Western-NBP3-06859-img0002.jpg)
![Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06859] - Simple Western performed with monoclonal HSP antibody, MAB1800, on Jurkat (human acute T cell leukemia), HeLa (human cervical cancer cell line), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1ug/ml dilution.](http://images.novusbio.com/fullsize/Loading-Control-Simple-Western-Antibody-Pack-Simple-Western-NBP3-06859-img0003.jpg)
![Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06859] - Simple Western performed with monoclonal alpha Tubulin antibody, NB100-690, on Jurkat (human acute T cell leukemia), HeLa (human cervical cancer cell line), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1ug/ml dilution.](http://images.novusbio.com/fullsize/Loading-Control-Simple-Western-Antibody-Pack-Simple-Western-NBP3-06859-img0004.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining of human colon, liver, lymph node and testis using Anti-PCNA antibody NBP1-89503 (A) shows similar protein distribution across tissues to independent antibody NBP1-89504 (B).](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-89503-img0018.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining of human colon, liver, lymph node and testis using Anti-PCNA antibody NBP1-89503 (A) shows similar protein distribution across tissues to independent antibody NBP1-89504 (B).")
![Immunoprecipitation: Histone H3 [ac Lys27] Antibody-Histone H3 [ac Lys27] Antibody (2V10N10)-[NBP3-15933] -](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-immunoprecipitation-622024101825..jpg "Immunoprecipitation: Histone H3 [ac Lys27] Antibody-Histone H3 [ac Lys27] Antibody (2V10N10)-[NBP3-15933] -")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody (2V10N10)- [NBP3-15933] - Analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-chromatin-immunoprecipitation-622024102155..jpg)
![Dot Blot-Histone H3 [ac Lys27] Antibody (2V10N10)-NBP3-15933- Analysis of all sorts of peptides using Acetyl-Histone H3-K27 Rabbit mAb at 1:200000 dilution.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-dot-blot-622024102348..jpg)
![<b>Biological Strategies Validation. </b>Western Blot: -Histone H3 [ac Lys27] Antibody (2V10N10)-NBP3-15933-analysis of lysates from C2C12 cells using Acetyl-Histone H3-K27 Rabbit mAb at 1:100000 dilution. C2C12 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 20 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-western-blot-622024102550..jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517213518.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-5220251726021.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517192955.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517284941.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunofluorescence analysis of HeLa treated with TSA and HeLa cells using Histone H3 Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517284953.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517202962.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517223970.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517273516.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-5220251723576.jpg)
![Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on L6 cell lysates with Rabbit anti-PCNA antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Western-Blot-NBP2-67390-img0010.jpg "Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on L6 cell lysates with Rabbit anti-PCNA antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse stomach tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0007.jpg)
![Flow Cytometry: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of Hela cells with PCNA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Flow-Cytometry-NBP2-67390-img0001.jpg)
![Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on different lysates using anti-PCNA antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: A431](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Western-Blot-NBP2-67390-img0008.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human breast carcinama tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0002.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0003.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0004.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse colon tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0005.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0006.jpg)
![Western blot analysis on different lysates with NBP2-67390 at 1/1,000 dilution. Lane 1: Mouse skin tissue lysate Lane 2: Mouse spleen tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 29 kDa Observed band size: 34 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67390 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67390_-western-blot-639204597789755461.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Analysis of H3K9me1 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Antibody-Dot-Blot-NB21-1071-img0009.jpg "Dot Blot: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Analysis of H3K9me1 in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-1071 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1071-img0011.jpg)
![Western Blot: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Analysis of H3K9me1 in NIH/3T3 histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Antibody-Western-Blot-NB21-1071-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Antibody at 1:50 in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1071-img0016.jpg)
![Western Blot: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - WB analysis of H3K9me1 in C. elegans embryo lysate. Observed molecular weight is ~12 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Antibody-Western-Blot-NB21-1071-img0013.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Analysis of H3 K36-Me2 using the peptides stated above in 10, 6, 3 and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Dot-Blot-NB21-1252-img0010.jpg "Dot Blot: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Analysis of H3 K36-Me2 using the peptides stated above in 10, 6, 3 and 1 picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 ug of NB21-1252 and 20 uL of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-NB21-1252-img0009.jpg)
![Western Blot: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Analysis of H3 K36Me2 in C. elegans embryo lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Western-Blot-NB21-1252-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Antibody was tested in Neuro2a cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1252-img0016.jpg)
![Western Blot: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Analysis of H3K36me2 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Western-Blot-NB21-1252-img0011.jpg)