| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1251AF405 | |||
| Alexa Fluor 488 | NB21-1251AF488 | |||
| Alexa Fluor 647 | NB21-1251AF647 | |||
| Alexa Fluor 700 | NB21-1251AF700 | |||
| Biotin | NB21-1251B | |||
| DyLight 405 | NB21-1251V | |||
| DyLight 488 | NB21-1251G | |||
| DyLight 550 | NB21-1251R | |||
| DyLight 650 | NB21-1251C | |||
| FITC | NB21-1251F | |||
| HRP | NB21-1251H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1251SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1141AF405 | |||
| Alexa Fluor 488 | NB21-1141AF488 | |||
| Alexa Fluor 647 | NB21-1141AF647 | |||
| Alexa Fluor 700 | NB21-1141AF700 | |||
| Biotin | NB21-1141B | |||
| DyLight 405 | NB21-1141V | |||
| DyLight 488 | NB21-1141G | |||
| DyLight 550 | NB21-1141R | |||
| DyLight 650 | NB21-1141C | |||
| FITC | NB21-1141F | |||
| HRP | NB21-1141H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1141SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1051AF405 | |||
| Alexa Fluor 488 | NB21-1051AF488 | |||
| Alexa Fluor 647 | NB21-1051AF647 | |||
| Alexa Fluor 700 | NB21-1051AF700 | |||
| Biotin | NB21-1051B | |||
| DyLight 405 | NB21-1051V | |||
| DyLight 488 | NB21-1051G | |||
| DyLight 550 | NB21-1051R | |||
| DyLight 650 | NB21-1051C | |||
| FITC | NB21-1051F | |||
| HRP | NB21-1051H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1051SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1037AF405 | |||
| Alexa Fluor 488 | NB21-1037AF488 | |||
| Alexa Fluor 647 | NB21-1037AF647 | |||
| Alexa Fluor 700 | NB21-1037AF700 | |||
| Biotin | NB21-1037B | |||
| DyLight 405 | NB21-1037V | |||
| DyLight 488 | NB21-1037G | |||
| DyLight 550 | NB21-1037R | |||
| DyLight 650 | NB21-1037C | |||
| FITC | NB21-1037F | |||
| HRP | NB21-1037H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1037SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1101AF405 | |||
| Alexa Fluor 488 | NB21-1101AF488 | |||
| Alexa Fluor 647 | NB21-1101AF647 | |||
| Alexa Fluor 700 | NB21-1101AF700 | |||
| Biotin | NB21-1101B | |||
| DyLight 405 | NB21-1101V | |||
| DyLight 488 | NB21-1101G | |||
| DyLight 550 | NB21-1101R | |||
| DyLight 650 | NB21-1101C | |||
| FITC | NB21-1101F | |||
| HRP | NB21-1101H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1101SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1053AF405 | |||
| Alexa Fluor 488 | NB21-1053AF488 | |||
| Alexa Fluor 647 | NB21-1053AF647 | |||
| Alexa Fluor 700 | NB21-1053AF700 | |||
| Biotin | NB21-1053B | |||
| DyLight 405 | NB21-1053V | |||
| DyLight 488 | NB21-1053G | |||
| DyLight 550 | NB21-1053R | |||
| DyLight 650 | NB21-1053C | |||
| FITC | NB21-1053F | |||
| HRP | NB21-1053H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1053SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB93441-SP | |||
![Western Blot: HDAC1 Antibody (2L6O7) [NBP3-15780] - Western blot analysis of extracts of various cell lines, using HDAC1 antibody (NBP3-15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2L6O7-Western-Blot-NBP3-15780-img0006.jpg "Western Blot: HDAC1 Antibody (2L6O7) [NBP3-15780] - Western blot analysis of extracts of various cell lines, using HDAC1 antibody (NBP3-15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody (2L6O7) [NBP3-15780] - Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (NBP3-15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2L6O7-Knockout-Validated-NBP3-15780-img0001.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517311064.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517304910.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517315592.jpg)
![Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody (2L6O7) [HDAC1] - Confocal imaging of HeLa cells using [KO Validated] HDAC1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-5220251733513.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517304961.jpg)
![Immunoprecipitation: HDAC1 Antibody (2L6O7) [HDAC1] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 0.5ug HDAC1 antibody . Western blot was performed from the immunoprecipitate using HDAC1 antibody at a dilition of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-5220251733586.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517304925.jpg)
![Immunoprecipitation: Histone H3 [ac Lys9] Antibody (PSH04-47) [NBP3-32430] - Histone H3 (acetyl K9) was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32430 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32430 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32430 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32430 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 18 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32430_rabbit-histone-h3-ac-lys9-mab-psh04-47-26820241582611.jpg "Immunoprecipitation: Histone H3 [ac Lys9] Antibody (PSH04-47) [NBP3-32430] - Histone H3 (acetyl K9) was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32430 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32430 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32430 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32430 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 18 seconds; ECL")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody (PSH04-47) [NBP3-32430] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and Histone H3 (NBP3-32430) / Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32430_rabbit-histone-h3-ac-lys9-mab-psh04-47-26820241525102.jpg)
![Dot Blot: Histone H3 [ac Lys9] Antibody (PSH04-47) [NBP3-32430] - Dot blot analysis of Histone H3 on different proteins with Rabbit anti-Histone H3 antibody (NBP3-32430) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified Histone H3 (negative) Lane 2: Mono-Methyl-Histone H3 (Lys9) (negative) Lane 3: Tri-Methyl-Histone H3 (Lys9) (negative) Lane 4: Acetyl-Histone H3 (Lys9) (positive) Lane 5: Acetyl-Histone H3 (Lys4) (negative) Lane 6: Acetyl-Histone H3 (Lys27) (negative) Proteins loading: 100ng, 25ng, 5ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 30 seconds; ECL.](http://images.novusbio.com/fullsize/nbp3-32430_rabbit-histone-h3-ac-lys9-mab-psh04-47-28820241459254.jpg)
![Immunohistochemistry: Histone H3 [ac Lys9] Antibody (PSH04-47) [NBP3-32430] - Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Histone H3 antibody (NBP3-32430) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32430_rabbit-histone-h3-ac-lys9-mab-psh04-47-298202414115442.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys9] Antibody (PSH04-47) [NBP3-32430] - Immunocytochemistry analysis of C6 cells labeling Histone H3 with Rabbit anti-Histone H3 antibody (NBP3-32430) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 antibody at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32430_rabbit-histone-h3-ac-lys9-mab-psh04-47-29202410234249.jpg)
![Flow Cytometry: Histone H3 [ac Lys9] Antibody (PSH04-47) [NBP3-32430] - Flow cytometric analysis of HeLa cells labeling Histone H3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32430, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32430_rabbit-histone-h3-ac-lys9-mab-psh04-47-2920241645328.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody (PSH04-47) [NBP3-32430] - Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (NBP3-32430) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate Lane 5: C6 cell lysate Lane 6: C6 treated with 1μM TSA for 18 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 6 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32430_rabbit-histone-h3-ac-lys9-mab-psh04-47-3920248453524.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys36] Antibody [NB21-1251] - Analysis of H3 K36me1 using the peptides stated in 10, 6, 3, 1, 0.6 and 0.3 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys36]-Antibody-Dot-Blot-NB21-1251-img0008.jpg "Dot Blot: Histone H3 [Monomethyl Lys36] Antibody [NB21-1251] - Analysis of H3 K36me1 using the peptides stated in 10, 6, 3, 1, 0.6 and 0.3 picomoles of peptide.")
![Western Blot: Histone H3 [Monomethyl Lys36] Antibody [NB21-1251] - Analysis of Histone H3 K36me1 in C. elegans embryo lysate. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys36]-Antibody-Western-Blot-NB21-1251-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys36] Antibody [NB21-1251] - Histone H3 K36me1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys36]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1251-img0013.jpg)
![Western Blot: Histone H3 [Monomethyl Lys36] Antibody [NB21-1251] - Analysis of H3 K36me1 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys36]-Antibody-Western-Blot-NB21-1251-img0009.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys18] Antibody [NB21-1141] - Analysis of HIstone H3K18me1 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys18]-Antibody-Dot-Blot-NB21-1141-img0006.jpg "Dot Blot: Histone H3 [Monomethyl Lys18] Antibody [NB21-1141] - Analysis of HIstone H3K18me1 in picomoles of peptide.")
![Western Blot: Histone H3 [Monomethyl Lys18] Antibody [NB21-1141] - Detection of H3K18me1 in HeLa histone prep lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys18]-Antibody-Western-Blot-NB21-1141-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys18] Antibody [NB21-1141] - Antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys18]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1141-img0011.jpg)
![Western Blot: Histone H3 [Monomethyl Lys18] Antibody [NB21-1141] - Analysis of Histone H3K18me1 in C. elegans embryo lysate. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys18]-Antibody-Western-Blot-NB21-1141-img0007.jpg)
![Dot Blot: Histone H3 [p Thr6] Antibody [NB21-1051] - Analysis of Histone H3 [phospho T6] using the peptides stated above in 1.0, 0.3, 0.1 and 0.03 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6]-Antibody-Dot-Blot-NB21-1051-img0008.jpg "Dot Blot: Histone H3 [p Thr6] Antibody [NB21-1051] - Analysis of Histone H3 [phospho T6] using the peptides stated above in 1.0, 0.3, 0.1 and 0.03 picomoles of peptide.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6] Antibody [NB21-1051] - Staining of Histone H3 [phospho T6] in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1051-img0013.jpg)
![Western Blot: Histone H3 [p Thr6] Antibody [NB21-1051] - Analysis of H3pt6 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6]-Antibody-Western-Blot-NB21-1051-img0010.jpg)
![Western Blot: Histone H3 [p Thr6] Antibody [NB21-1051] - Detection of Histone H3 [phospho T6] in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6]-Antibody-Western-Blot-NB21-1051-img0009.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4, p Thr6] Antibody [NB21-1037] - Aanalysis of H3K4me1/pt6 in C. elegans embryonic lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4-p-Thr6]-Antibody-Western-Blot-NB21-1037-img0004.jpg "Western Blot: Histone H3 [Monomethyl Lys4, p Thr6] Antibody [NB21-1037] - Aanalysis of H3K4me1/pt6 in C. elegans embryonic lysate. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys4, p Thr6] Antibody [NB21-1037] - Staining of H3K4me1/pT6 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4--p-Thr6]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1037-img0007.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4, p Thr6] Antibody [NB21-1037] - Detection of H3K4me1/pT6 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4-p-Thr6]-Antibody-Western-Blot-NB21-1037-img0001.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys4, p Thr6] Antibody - BSA Free [NB21-1037] - Analysis of H3K4me1/pT6 antibody in picomoles of protein.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4--p-Thr6]-Antibody---BSA-Free-Dot-Blot-NB21-1037-img0008.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-54614] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27me3 diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Western-Blot-NBP2-54614-img0013.jpg "Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-54614] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27me3 diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-54614] - Human HeLa cells were stained with the antibody against H3K27me3 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/Triton X-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K27me3 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54614-img0012.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys27] Antibody [NBP2-54614] - Recovery of the nucleosomes carrying the H3K27me1, H3K27me2, H3K27me3, H3K4me3, H3K9me3 and H3K36me3 modifications and the unmodified H3K27 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K27me3 modification.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Chromatin-Immunoprecipitation-NBP2-54614-img0015.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys27] Antibody [NBP2-54614] - ChIP assays were performed using human HeLa cells, the antibody against H3K27me3 and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. The chromatin was spiked with a panel of in vitro assembled nucleosomes, each containing a specific lysine methylation (SNAP-ChIP K-MetStat Panel, Epicypher). A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active GAPDH and EIF4A2 genes, used as negative controls, and for the inactive TSH2B and MYT1 genes, used as positive controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Chromatin-Immunoprecipitation-NBP2-54614-img0014.jpg)
![ELISA: Histone H3 [Trimethyl Lys27] Antibody [NBP2-54614] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H3K27me3. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:3,000.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-ELISA-NBP2-54614-img0008.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-54614] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K27me3 with peptides containing other modifications of histone H3 and H4 and the unmodified H3K27 sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:5,000. The figure shows a high specificity of the antibody for the modification of interest. Please note that the antibody also recognizes the modification if S28 is phosphorylated.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Dot-Blot-NBP2-54614-img0009.jpg)
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody (7W5T2) [NBP3-15921] - Western blot analysis of extracts of various cell lines, using Histone H3 antibody (NBP3-15921) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-7W5T2-Western-Blot-NBP3-15921-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys36] Antibody (7W5T2) [NBP3-15921] - Western blot analysis of extracts of various cell lines, using Histone H3 antibody (NBP3-15921) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Dot Blot: Histone H3 [Trimethyl Lys36] Antibody (7W5T2) [NBP3-15921] - Dot-blot analysis of all sorts of peptides using Histone H3 antibody (NBP3-15921) at 1:1000 dilution.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-7W5T2-Dot-Blot-NBP3-15921-img0002.jpg)
![Immunohistochemistry: Histone H3 [Trimethyl Lys36] Antibody (7W5T2) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Histone H3 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15921_rabbit-histone-h3-trimethyl-lys-36-mab-7w5t2-5220251725196.jpg)
![Immunohistochemistry: Histone H3 [Trimethyl Lys36] Antibody (7W5T2) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Histone H3 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15921_rabbit-histone-h3-trimethyl-lys-36-mab-7w5t2-52202517251922.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody (7W5T2) [Histone H3] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and H3K36me3 Rabbit mAb . The ChIP sequencing results indicate the enrichment pattern of H3K36me3 in selected genomic region and representative gene loci (GAPDH), as shown in figure.](http://images.novusbio.com/fullsize/nbp3-15921_rabbit-histone-h3-trimethyl-lys-36-mab-7w5t2-52202517202926.jpg)
![Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody (7W5T2) [Histone H3] - Immunoprecipitation analysis of 600 ug extracts of 293F cells using 5 ug Histone H3 antibody . Western blot was performed from the immunoprecipitate using Histone H3 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15921_rabbit-histone-h3-trimethyl-lys-36-mab-7w5t2-52202517251960.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody (7W5T2) [Histone H3] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H3 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15921_rabbit-histone-h3-trimethyl-lys-36-mab-7w5t2-52202517213568.jpg)
![Dot Blot: Histone H3 [p Thr11] Antibody [NB21-1101] - Analysis of H3pT11 with the peptides stated above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Dot-Blot-NB21-1101-img0003.jpg "Dot Blot: Histone H3 [p Thr11] Antibody [NB21-1101] - Analysis of H3pT11 with the peptides stated above in picomoles of peptide.")
![Western Blot: Histone H3 [p Thr11] Antibody [NB21-1101] - Analysis of H3pT11 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Western-Blot-NB21-1101-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr11] Antibody [NB21-1101] - Staining of H3pT11 in HeLa cells using FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1101-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H3 [p Thr6, Dimethyl Lys9] Antibody [NB21-1053] - 2 ug of NB21-1053 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Dimethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1053-img0008.jpg "Chromatin Immunoprecipitation: Histone H3 [p Thr6, Dimethyl Lys9] Antibody [NB21-1053] - 2 ug of NB21-1053 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).")
![Western Blot: Histone H3 [p Thr6, Dimethyl Lys9] Antibody [NB21-1053] - Analysis of H3K9me2/pT6 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9-p-Thr6]-Antibody-Western-Blot-NB21-1053-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6, Dimethyl Lys9] Antibody [NB21-1053] - Histone H3.2 K9me2 pT6 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Dimethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1053-img0007.jpg)
![Western Blot: alpha Tubulin Antibody (961258) [MAB93441] - Detection of Human, Mouse, and Rat alpha Tubulin by Western Blot. Western blot shows lysates of HeLa, Jurkat, NIH-3T3, Neuro2A and Rat-2 cell lines. PVDF membrane was probed with Mouse alpha Tubulin Antibody (961258) (Catalog # MAB93441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha Tubulin at an approximate molecular weight of 50 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-961258-Western-Blot-MAB93441-img0004.jpg "Western Blot: alpha Tubulin Antibody (961258) [MAB93441] - Detection of Human, Mouse, and Rat alpha Tubulin by Western Blot. Western blot shows lysates of HeLa, Jurkat, NIH-3T3, Neuro2A and Rat-2 cell lines. PVDF membrane was probed with Mouse alpha Tubulin Antibody (961258) (Catalog # MAB93441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha Tubulin at an approximate molecular weight of 50 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody (961258) [MAB93441] - alpha Tubulin was detected in immersion fixed paraffin-embedded sections of human brin hippocampus tissue using alpha Tubulin Antibody (961258) at 5 ug/ml. Tissue was stained using VC001 (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm in cancer cells.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-961258-Immunohistochemistry-Paraffin-MAB93441-img0002.jpg)
![Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06858] - Simple Western performed with GAPDH antibody, 2275-PC, on Jurkat (human acute T cell leukemia), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1:500 dilution.](http://images.novusbio.com/fullsize/Loading-Control-Simple-Western-Antibody-Pack-Simple-Western-NBP3-06858-img0001.jpg "Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06858] - Simple Western performed with GAPDH antibody, 2275-PC, on Jurkat (human acute T cell leukemia), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1:500 dilution.")
![Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06858] - Simple Western performed with HSP60 antibody, AF1800, on Jurkat (human acute T cell leukemia), HeLa (human cervical cancer cell line), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1ug/ml dilution.](http://images.novusbio.com/fullsize/Loading-Control-Simple-Western-Antibody-Pack-Simple-Western-NBP3-06858-img0002.jpg)
![Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06858] - Simple Western performed with beta-Actin antibody, NB600-503, on Jurkat (human acute T cell leukemia), HeLa (human cervical cancer cell line), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1:50 dilution.](http://images.novusbio.com/fullsize/Loading-Control-Simple-Western-Antibody-Pack-Simple-Western-NBP3-06858-img0003.jpg)
![Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06858] - Simple Western performed with monoclonal beta Tubulin antibody, NB600-936, on HeLa (human cervical cancer cell line), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1ug/ml dilution.](http://images.novusbio.com/fullsize/Loading-Control-Simple-Western-Antibody-Pack-Simple-Western-NBP3-06858-img0004.jpg)