| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1054AF405 | |||
| Alexa Fluor 488 | NB21-1054AF488 | |||
| Alexa Fluor 647 | NB21-1054AF647 | |||
| Alexa Fluor 700 | NB21-1054AF700 | |||
| Biotin | NB21-1054B | |||
| DyLight 405 | NB21-1054V | |||
| DyLight 488 | NB21-1054G | |||
| DyLight 550 | NB21-1054R | |||
| DyLight 650 | NB21-1054C | |||
| FITC | NB21-1054F | |||
| HRP | NB21-1054H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1054SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1039AF405 | |||
| Alexa Fluor 488 | NB21-1039AF488 | |||
| Alexa Fluor 647 | NB21-1039AF647 | |||
| Alexa Fluor 700 | NB21-1039AF700 | |||
| Biotin | NB21-1039B | |||
| DyLight 405 | NB21-1039V | |||
| DyLight 488 | NB21-1039G | |||
| DyLight 550 | NB21-1039R | |||
| DyLight 650 | NB21-1039C | |||
| FITC | NB21-1039F | |||
| HRP | NB21-1039H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1039SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43962 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44032 | |||
| BSA Free | NBP2-52937-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89503-25ul | |||
![Dot Blot: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Analysis of H3K9me3/pT6 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Dot-Blot-NB21-1054-img0010.jpg "Dot Blot: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Analysis of H3K9me3/pT6 in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1054 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1054-img0013.jpg)
![Western Blot: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Analysis of H3K9me3/pT6 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Western-Blot-NB21-1054-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Histone H3.2 K9me3 pT6 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1054-img0016.jpg)
![Western Blot: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Analysis of H3K9Me3/pT6 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Western-Blot-NB21-1054-img0012.jpg)
![Dot Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - Dot blot analysis of H3K4me3/pT6 with the peptides stated above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Dot-Blot-NB21-1039-img0010.jpg "Dot Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - Dot blot analysis of H3K4me3/pT6 with the peptides stated above in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - 2 ug of NB21-1039 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control, DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1039-img0014.jpg)
![Western Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - WB analysis of H3K4Me3/pT6 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Western-Blot-NB21-1039-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - Histone H3.2 K4me3 pT6 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1039-img0017.jpg)
![Western Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - WB detection of H3K4me3/pT6 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Western-Blot-NB21-1039-img0011.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M14), clone 5C11. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0016.jpg "Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M14), clone 5C11. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of monoclonal antibody to HDAC1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Immunocytochemistry-Immunofluorescence-H00003065-M14-img0009.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Immunohistochemistry-Paraffin-H00003065-M14-img0010.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0012.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0013.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0014.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0015.jpg)
![ELISA: HDAC1 Antibody (5C11) [H00003065-M14] - Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-ELISA-H00003065-M14-img0008.jpg)
![Proximity Ligation Assay: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of protein-protein interactions between NFKB1 and HDAC1. HeLa cells were stained with anti-NFKB1 rabbit purified polyclonal 1:1200 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Proximity-Ligation-Assay-H00003065-M14-img0011.jpg)
![Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on L6 cell lysates with Rabbit anti-PCNA antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Western-Blot-NBP2-67390-img0010.jpg "Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on L6 cell lysates with Rabbit anti-PCNA antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse stomach tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0007.jpg)
![Flow Cytometry: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of Hela cells with PCNA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Flow-Cytometry-NBP2-67390-img0001.jpg)
![Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on different lysates using anti-PCNA antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: A431](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Western-Blot-NBP2-67390-img0008.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human breast carcinama tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0002.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0003.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0004.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse colon tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0005.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0006.jpg)
![Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06859] - Simple Western performed with monoclonal GAPDH antibody, MAB5718, on Jurkat (human acute T cell leukemia), HeLa (human cervical cancer cell line), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1ug/ml dilution.](http://images.novusbio.com/fullsize/Loading-Control-Simple-Western-Antibody-Pack-Simple-Western-NBP3-06859-img0001.jpg "Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06859] - Simple Western performed with monoclonal GAPDH antibody, MAB5718, on Jurkat (human acute T cell leukemia), HeLa (human cervical cancer cell line), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1ug/ml dilution.")
![Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06859] - Simple Western performed with monoclonal beta-Actin antibody, MAB8929, on Jurkat (human acute T cell leukemia), HeLa (human cervical cancer cell line), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1ug/ml dilution.](http://images.novusbio.com/fullsize/Loading-Control-Simple-Western-Antibody-Pack-Simple-Western-NBP3-06859-img0002.jpg)
![Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06859] - Simple Western performed with monoclonal HSP antibody, MAB1800, on Jurkat (human acute T cell leukemia), HeLa (human cervical cancer cell line), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1ug/ml dilution.](http://images.novusbio.com/fullsize/Loading-Control-Simple-Western-Antibody-Pack-Simple-Western-NBP3-06859-img0003.jpg)
![Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06859] - Simple Western performed with monoclonal alpha Tubulin antibody, NB100-690, on Jurkat (human acute T cell leukemia), HeLa (human cervical cancer cell line), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1ug/ml dilution.](http://images.novusbio.com/fullsize/Loading-Control-Simple-Western-Antibody-Pack-Simple-Western-NBP3-06859-img0004.jpg)
![Western Blot: COX4 Antibody (CL3497) [NBP2-78718] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Western-Blot-NBP2-78718-img0002.jpg "Western Blot: COX4 Antibody (CL3497) [NBP2-78718] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human prostate shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: COX4 Antibody (CL3497) [NBP2-78718] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-COX4I1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Western-Blot-NBP2-78718-img0001.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human fallopian tube shows strong cytoplasmic positivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0003.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human stomach shows strong cytoplasmic immunoreactivity in glandular epithelium cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0004.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0005.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules and glomerulus cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0006.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Analysis in human rectum and skeletal muscle tissues using NBP2-52937 antibody. Corresponding HDAC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunohistochemistry-Paraffin-NBP2-52937-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Analysis in human rectum and skeletal muscle tissues using NBP2-52937 antibody. Corresponding HDAC1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: HDAC1 Antibody (CL0510) [NBP2-52937] - analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HDAC1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Western-Blot-NBP2-52937-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of U-251 cells using the Anti-HDAC1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunocytochemistry-Immunofluorescence-NBP2-52937-img0010.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunohistochemistry-Paraffin-NBP2-52937-img0015.jpg)
![Western Blot: HDAC1 Antibody (CL0510) [NBP2-52937] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Western-Blot-NBP2-52937-img0008.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunohistochemistry-Paraffin-NBP2-52937-img0012.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunohistochemistry-Paraffin-NBP2-52937-img0013.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunohistochemistry-Paraffin-NBP2-52937-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-89503] - Staining of human cell line U-2 OS shows localization to nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89503-img0011.jpg "Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-89503] - Staining of human cell line U-2 OS shows localization to nuclear bodies. Antibody staining is shown in green.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining of human colon, liver, lymph node and testis using Anti-PCNA antibody NBP1-89503 (A) shows similar protein distribution across tissues to independent antibody NBP1-89504 (B).](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-89503-img0018.jpg)
![Western Blot: Histone H3 [ac Lys27] Antibody [NBP2-54615] - Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Western Blot: Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-3-[Acetylated-Lys27]-[ac-Lys27]-Antibody-Western-Blot-NBP2-54615-img0007.jpg "Western Blot: Histone H3 [ac Lys27] Antibody [NBP2-54615] - Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Western Blot: Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody [NBP2-54615] - HeLa cells were stained with the antibody against H3K27ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/ TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K27ac antibody (top) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown at the bottom.](http://images.novusbio.com/fullsize/Histone-3-[Acetylated-Lys27]-[ac-Lys27]-Antibody-Immunofluorescence-NBP2-54615-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody [NBP2-54615] - ChIP assays were performed using human K562 cells, the antibody against H3K27ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 100,000 cells. A titration consisting of 0.2, 0.5, 1 and 2 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active GAPDH and EIF4A2 genes, used as positive controls, and for the coding regions of the inactive MB and MYT1 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis)](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54615-img0010.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody [NBP2-54615] - ChIP assays were performed using human HeLa cells, the antibody against H3K27ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and ACTB genes, used as positive controls, and for the inactive TSH2B and MYT1 genes, used as negative controls.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54615-img0009.jpg)
![Dot Blot: Histone H3 [ac Lys27] Antibody [NBP2-54615] - Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Dot Blot: Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - To test the cross reactivity of the antibody against H3K27ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K27. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-3-[Acetylated-Lys27]-[ac-Lys27]-Antibody-Dot-Blot-NBP2-54615-img0008.jpg)
![ELISA: Histone H3 [ac Lys27] Antibody [NBP2-54615] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K27ac The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,300.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys27]-Antibody-ELISA-NBP2-54615-img0011.jpg)
![Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K4me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-3-[Tryimethyl-Lys4]-Antibody-Western-Blot-NBP2-54610-img0003.jpg "Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K4me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Immunohistochemistry: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Histone 3 [Tryimethyl Lys4] Antibody [NBP2-54610] - HeLa cells were stained with the antibody against H3K4me3 and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K4me3 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-3-[Tryimethyl-Lys4]-Antibody-Immunohistochemistry-NBP2-54610-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Histone 3 [Tryimethyl Lys4] [Trimethyl Lys4] Antibody [NBP2-54610] - ChIP assays were performed using human HeLa cells, the antibody against H3K4me3, and optimized PCR primer pairs for qPCR. Figure shows the recovery, expressed as a percent of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that trimethylation of K4 at histone H3 is associated with the promoters of active genes.](http://images.novusbio.com/fullsize/Histone-3-Tryimethyl-Lys4-Trimethyl-Lys4-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54610-img0005.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Histone 3 [Tryimethyl Lys4] Antibody [NBP2-54610] - One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-3-[Tryimethyl-Lys4]-Antibody-Dot-Blot-NBP2-54610-img0002.jpg)
![ELISA: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Histone 3 [Tryimethyl Lys4] [Trimethyl Lys4] Antibody [NBP2-54610] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K4me3. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:18,350.](http://images.novusbio.com/fullsize/Histone-3-[Tryimethyl-Lys4]-[Trimethyl-Lys4]-Antibody-ELISA-NBP2-54610-img0006.jpg)
![Protein Array: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Histone 3 [Tryimethyl Lys4] [Trimethyl Lys4] Antibody [NBP2-54610] - The specificity of the antibody was further demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:10,000. Figure shows a high specificity for the peptides containing the H3K4me3 modification.](http://images.novusbio.com/fullsize/Histone-3-[Tryimethyl-Lys4]-[Trimethyl-Lys4]-Antibody-Protein-Array-NBP2-54610-img0007.jpg)
![Western Blot: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody directed against H3K9/14ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-Western-Blot-NBP2-59181-img0001.jpg "Western Blot: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody directed against H3K9/14ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - ChIP assays were performed using HeLa cells, the antibody against H3K9/14ac and optimized primer pairs for qPCR. ChIP was performed with a ChIP kit, using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control.QPCR was performed using primers specific for the promoter of the ACTB gene as a positive control target and for exon 2 of the MB gene as a negative control target. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA). These results confirm the observation that acetylation of H3K9/14 is present at active promoters.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59181-img0008.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - ChIP assays were performed using HeLa cells, the antibody against H3K9/14ac and optimized primer pairs for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed using primers specific for the promoter of the ACTB gene as a positive control target and for exon 2 of the MB gene as a negative control target. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA). These results confirm the observation that acetylation of H3K9/14 is present at active promoters.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59181-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - ChIP was performed with 1 ug of the antibody against H3K9/14ac on sheared chromatin from 1 million HeLaS3 cells. IgG (2 ug/IP) was used as a negative IP control. The IP'd DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative control targets.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59181-img0005.jpg)
![Dot Blot: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K9/14ac with peptides containing other histone modifications and the unmodified H3K9/14 sequence. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-Dot-Blot-NBP2-59181-img0002.jpg)
![ELISA: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K9/14ac, crude serum and flow through in antigen coated wells. The antigen used was a peptie containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:5,900.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-ELISA-NBP2-59181-img0003.jpg)
![Immunofluorescence: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - Mouse NIH3T3 cells were stained with the antibody against H3K9/14ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9/14ac antibody (left) diluted 1:500 in blocking solution followed by an anti- rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14-ac-Lys9]-Antibody-Immunofluorescence-NBP2-59181-img0006.jpg)
![Western Blot: HDAC1 Antibody (2L6O7) [NBP3-15780] - Western blot analysis of extracts of various cell lines, using HDAC1 antibody (NBP3-15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2L6O7-Western-Blot-NBP3-15780-img0006.jpg "Western Blot: HDAC1 Antibody (2L6O7) [NBP3-15780] - Western blot analysis of extracts of various cell lines, using HDAC1 antibody (NBP3-15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody (2L6O7) [NBP3-15780] - Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (NBP3-15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2L6O7-Knockout-Validated-NBP3-15780-img0001.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517311064.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517304910.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517315592.jpg)
![Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody (2L6O7) [HDAC1] - Confocal imaging of HeLa cells using [KO Validated] HDAC1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-5220251733513.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517304961.jpg)
![Immunoprecipitation: HDAC1 Antibody (2L6O7) [HDAC1] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 0.5ug HDAC1 antibody . Western blot was performed from the immunoprecipitate using HDAC1 antibody at a dilition of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-5220251733586.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517304925.jpg)