| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-93479SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73394 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73005 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73393 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-95251-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95253-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93423-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89744-25ul | |||
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free [NB110-93479] - Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - MEF were treated with 10 uM BA or DMSO (0.1%) for 16 h before they were subjected to a glycolysis stress test as described under](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-[p-Ser293]-Antibody-Western-Blot-NB110-93479-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free [NB110-93479] - Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - MEF were treated with 10 uM BA or DMSO (0.1%) for 16 h before they were subjected to a glycolysis stress test as described under")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] antibody (1:250) was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-[p-Ser293]-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93479-img0006.jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - Detection of PDHE1 alpha [S293] in an in vitro autophosphorylation of PDH complex in response to different stimulants: (-) none, H202-hydrogen peroxide, AR-anhydroretinol, Rol-retinol, RA-retinoic acid, PMA, Lipid-PKC lipid activator. Photo courtesy of Dr. Beatrice Hoyos, Memorial Sloan-Kettering Cancer Center.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-[p-Ser293]-Antibody-Western-Blot-NB110-93479-img0003.jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - PDH activity and protein levels of PDK4 and p-PDH in hindlimb skeletal muscle. Samples were resolved by electrophoresis, transferred and probed by antibodies and representative immunoblots and normalization of pPDH are presented. The following comparisons were made: within a genotype, fed versus fasted; across genotypes, fed versus fed and fasted versus fasted. Bars that do not share a common symbol differ significantly (P < 0.05). Data presented as mean +/- SEM (n = 6). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0124204), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-[p-Ser293]-Antibody-Western-Blot-NB110-93479-img0008.jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free [NB110-93479] - PDK4 expression during syncytialization of human placental trophoblasts. (A) Changes of the RPKM values for PDK family members in trophoblasts before (red column, 3 hours) & after (blue column, 48 hours) syncytialization. (B) Changes in PDK4 mRNA (black column, n = 4) & protein (white column, n = 4) abundance during syncytialization. (C) Changes in the phosphorylation of PDHE1 alpha during syncytialization n = 4. (D) Representative images showing intense staining of PDK4 (red) in the cytotrophoblast layer & weak staining of PDK4 in the syncytial layer of human chorionic villi at early gestation. The syncytial & cytotrophoblast layers were labeled with beta -hCG (green) & SPINT1 (green) respectively. Nuclei were counterstained with DAPI (blue). n = 3; *P < 0.05; **P < 0.01; ***P < 0.001 against 3 hours; n.s., not significant. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-09163-8), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-93479_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-antibody-310202416212313.jpg)
![Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Western-Blot-NBP2-02056-img0026.jpg "Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Human liver tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0023.jpg)
![Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Phosphoribosyl Pyrophosphate Amidotransferase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Western-Blot-NBP2-02056-img0013.jpg)
![Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Phosphoribosyl Pyrophosphate Amidotransferase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Phosphoribosyl Pyrophosphate Amidotransferase.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Western-Blot-NBP2-02056-img0025.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0014.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0015.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0018.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Human colon tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0021.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Human Kidney tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0022.jpg)
![Western Blot: NME4 Antibody (1A5) [NBP2-02487] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NME4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NME4.](http://images.novusbio.com/fullsize/NME4-Antibody-1A5-Western-Blot-NBP2-02487-img0002.jpg "Western Blot: NME4 Antibody (1A5) [NBP2-02487] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NME4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NME4.")
![Immunocytochemistry/Immunofluorescence: NME4 Antibody (1A5) [NBP2-02487] Staining of COS7 cells transiently transfected by pCMV6-ENTRY NME4.](http://images.novusbio.com/fullsize/NME4-Antibody-1A5-Immunocytochemistry-Immunofluorescence-NBP2-02487-img0009.jpg)
![Immunohistochemistry-Paraffin: NME4 Antibody (1A5) [NBP2-02487] - Staining of paraffin-embedded liver tissue using anti-NME4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NME4-Antibody-1A5-Immunohistochemistry-Paraffin-NBP2-02487-img0008.jpg)
![Flow Cytometry: NME4 Antibody (1A5) [NBP2-02487] - HEK293T cells transfected with either pCMV6-ENTRY NME4.(Red) or empty vector control plasmid (Blue) were immunostained with anti-NME4 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/NME4-Antibody-1A5-Flow-Cytometry-NBP2-02487-img0001.jpg)
![Immunohistochemistry-Paraffin: NME4 Antibody (1A5) [NBP2-02487] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-NME4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NME4-Antibody-1A5-Immunohistochemistry-Paraffin-NBP2-02487-img0003.jpg)
![Immunohistochemistry-Paraffin: NME4 Antibody (1A5) [NBP2-02487] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NME4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NME4-Antibody-1A5-Immunohistochemistry-Paraffin-NBP2-02487-img0004.jpg)
![Immunohistochemistry-Paraffin: NME4 Antibody (1A5) [NBP2-02487] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-NME4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NME4-Antibody-1A5-Immunohistochemistry-Paraffin-NBP2-02487-img0005.jpg)
![Immunohistochemistry-Paraffin: NME4 Antibody (1A5) [NBP2-02487] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-NME4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NME4-Antibody-1A5-Immunohistochemistry-Paraffin-NBP2-02487-img0006.jpg)
![Immunohistochemistry-Paraffin: NME4 Antibody (1A5) [NBP2-02487] - Staining of paraffin-embedded Kidney tissue using anti-NME4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NME4-Antibody-1A5-Immunohistochemistry-Paraffin-NBP2-02487-img0007.jpg)
![Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B11) [NBP2-45379] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Phosphoribosyl Pyrophosphate Amidotransferase.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-1B11-Western-Blot-NBP2-45379-img0009.jpg "Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B11) [NBP2-45379] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Phosphoribosyl Pyrophosphate Amidotransferase.")
![Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B11) [NBP2-45379] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-1B11-Immunohistochemistry-NBP2-45379-img0008.jpg)
![Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B11) [NBP2-45379] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-1B11-Immunohistochemistry-NBP2-45379-img0001.jpg)
![Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B11) [NBP2-45379] - Analysis of Human colon tissue (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-1B11-Immunohistochemistry-NBP2-45379-img0002.jpg)
![Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B11) [NBP2-45379] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-1B11-Immunohistochemistry-NBP2-45379-img0003.jpg)
![Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B11) [NBP2-45379] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-1B11-Immunohistochemistry-NBP2-45379-img0004.jpg)
![Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B11) [NBP2-45379] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-1B11-Immunohistochemistry-NBP2-45379-img0005.jpg)
![Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B11) [NBP2-45379] - Analysis of Adenocarcinoma of Human ovary tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-1B11-Immunohistochemistry-NBP2-45379-img0006.jpg)
![Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (1B11) [NBP2-45379] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-1B11-Immunohistochemistry-NBP2-45379-img0007.jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-33922] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-Antibody-Western-Blot-NBP2-33922-img0007.jpg "Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-33922] - Analysis in human cell line HEK 293.")
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-33922] - PDHA2 Antibody [NBP2-33922] - Staining of human cell line HeLa shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33922-img0005.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-33922] -Staining of human heart muscle shows strong cytoplasm granular positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/antibody/nbp2-33922_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-immunohistochemistry-paraffin-174202494855..jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-33922] - Staining of human kidney shows strong cytoplasm granular positivity in cells in tubules.](http://images.novusbio.com/fullsize/antibody/nbp2-33922_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-immunohistochemistry-paraffin-17420249518..jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-33922] - Staining of human testis shows strong cytoplasm granular positivity in Leydig cells.](http://images.novusbio.com/fullsize/antibody/nbp2-33922_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-immunohistochemistry-paraffin-174202495223..jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-33922] - Staining of human duodenum shows moderate cytoplasm granular positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-33922_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-immunohistochemistry-paraffin-174202495338..jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95251] - Analysis of extracts of various cell lines, using PDHA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-Antibody-Western-Blot-NBP2-95251-img0007.jpg "Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95251] - Analysis of extracts of various cell lines, using PDHA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95251] - Human thyroid cancer using PDHA1 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-95251-img0006.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95251] - Rat kidney using PDHA1 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-95251-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit Antibody - Azide and BSA Free [NBP2-95251] - Immunofluorescence analysis of NIH/3T3 cells using Pyruvate Dehydrogenase E1-alpha subunit Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95251_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-5220251640291.jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] - analysis of various lysates, using PDHA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.5s.](http://images.novusbio.com/fullsize/nbp2-95253_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-western-blot-241202412935..jpg "Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] - analysis of various lysates, using PDHA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.5s.")
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] -Analysis of paraffin-embedded rat kidney using PDHA1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp2-95253_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-immunohistochemistry-paraffin-2412024121118..jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] - Human thyroid cancer using PDHA1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp2-95253_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-immunohistochemistry-paraffin-2412024121229..jpg)
![Western Blot: NME6 Antibody [NBP2-93423] - Western blot analysis of extracts of various cell lines, using NME6 Rabbit pAb (NBP2-93423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.](http://images.novusbio.com/fullsize/NME6 Antibody-Western Blot-NBP2-93423-img0004.jpg "Western Blot: NME6 Antibody [NBP2-93423] - Western blot analysis of extracts of various cell lines, using NME6 Rabbit pAb (NBP2-93423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.")
![Immunohistochemistry-Paraffin: NME6 Antibody [NBP2-93423] - Paraffin-embedded mouse kidney using NME6 .](http://images.novusbio.com/fullsize/NME6-Antibody-Immunohistochemistry-Paraffin-NBP2-93423-img0002.jpg)
![Immunohistochemistry-Paraffin: NME6 Antibody [NBP2-93423] - Paraffin-embedded human esophageal using NME6 .](http://images.novusbio.com/fullsize/NME6-Antibody-Immunohistochemistry-Paraffin-NBP2-93423-img0001.jpg)
![Immunohistochemistry-Paraffin: NME6 Antibody [NBP2-93423] - Paraffin-embedded rat pancreas using NME6 .](http://images.novusbio.com/fullsize/NME6-Antibody-Immunohistochemistry-Paraffin-NBP2-93423-img0003.jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Western blot analysis of Pyruvate Dehydrogenase E1-alpha subunit on different lysates with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HEK-293 cell lysate (20 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32787) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-288202412442923.jpg "Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Western blot analysis of Pyruvate Dehydrogenase E1-alpha subunit on different lysates with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HEK-293 cell lysate (20 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32787) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32787) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-298202414115418.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Immunocytochemistry analysis of Jurkat cells treated with or without lambda pp labeling Pyruvate Dehydrogenase E1-alpha subunit with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-2920241114815.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody [NBP1-89744] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-Immunohistochemistry-Paraffin-NBP1-89744-img0006.jpg "Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody [NBP1-89744] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.")
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody [NBP1-89744] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-Immunohistochemistry-Paraffin-NBP1-89744-img0007.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody [NBP1-89744] - Staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-Immunohistochemistry-Paraffin-NBP1-89744-img0005.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line MOLT-4](http://images.novusbio.com/fullsize/nbp1-89744_rabbit-polyclonal-phosphoribosyl-pyrophosphate-amidotransferase-antibody-13520251661869.jpg)
![Immunoprecipitation: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (9S3I4) [NBP3-16916] - Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-16916). Western blot was performed from the immunoprecipitate using Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-16916) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-p-Ser293-Antibody-9S3I4-Immunoprecipitation-NBP3-16916-img0002.jpg "Immunoprecipitation: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (9S3I4) [NBP3-16916] - Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-16916). Western blot was performed from the immunoprecipitate using Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-16916) at a dilition of 1:1000.")
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (9S3I4) [NBP3-16916] -analysis of lysates from HeLa cells, using Phospho-PDHA1-S293 Rabbit mAb at1:23000 dilution.NIH/3T3 cells were treated by CIP (20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16916_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser-293-mab-6e3j0-western-blot-75202483541..jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (9S3I4) [NBP3-16916] -analysis of lysates from HeLa cells, using Phospho-PDHA1-S293 Rabbit mAb at 1:23000 dilution.293T cells were treated by lambda -PP mixed solution (1ul) at 30 c for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25uμg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16916_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser-293-mab-6e3j0-western-blot-75202483713..jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (6E3J0) [NBP3-16916] - Western blot analysis of lysates from HeLa cells, using Pyruvate Dehydrogenase E1-alpha subunit Rabbit mAb at 1:23000 dilution.293T cells were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16916_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser-293-mab-6e3j0-52202511383736.jpg)