| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95146-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04711-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38299-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50387-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-75460-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89345-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89517-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97978-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89460-50ul | |||
![Western Blot: xCT Antibody - Azide and BSA Free [xCT] - Western blot analysis of lysates from Rat brain using xCT Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp2-95146_rabbit-polyclonal-xct-antibody-5220251628035.jpg "Western Blot: xCT Antibody - Azide and BSA Free [xCT] - Western blot analysis of lysates from Rat brain using xCT Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Western Blot: MAT1A Antibody - BSA Free [NBP3-04711] - Western blot analysis of Rat liver, using MAT1A Rabbit pAb (A6650) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-04711_rabbit-polyclonal-mat1a-antibody-52202519294539.jpg "Western Blot: MAT1A Antibody - BSA Free [NBP3-04711] - Western blot analysis of Rat liver, using MAT1A Rabbit pAb (A6650) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.")
![Western Blot: GPD2 Antibody [NBP3-38299] - Western blot analysis of various lysates using GPD2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38299_rabbit-gpd2-pab-101220241136080.jpg "Western Blot: GPD2 Antibody [NBP3-38299] - Western blot analysis of various lysates using GPD2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Immunohistochemistry: Cytochrome P450 3A5 Antibody (F18 P3 B6) [NBP3-30887] - A. and B. Western blot using 3A5 antibody (Cat. No. NBP3-30887) on recombinant CYP450 proteins.C. Immunohistochemistry stain using 3A5 antibody on oesophagus cancer tissue.](http://images.novusbio.com/fullsize/nbp3-30887_mouse-cytochrome-p450-3a5-mab-f18-p3-b6-811202420592140.jpg "Immunohistochemistry: Cytochrome P450 3A5 Antibody (F18 P3 B6) [NBP3-30887] - A. and B. Western blot using 3A5 antibody (Cat. No. NBP3-30887) on recombinant CYP450 proteins.C. Immunohistochemistry stain using 3A5 antibody on oesophagus cancer tissue.")
![Western Blot: IRP2 Antibody (9D1-R) [NBP3-32483] - Western blot analysis of IRP2 on different lysates with Mouse anti-IRP2 antibody (NBP3-32483) at 1/2,000 dilution. Lane 1: Daudi cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: Mouse liver tissue lysate (40 ug/Lane) Lane 4: Mouse kidney tissue lysate (40 ug/Lane) Lane 5: Mouse heart tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32483) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32483_mouse-irp2-mab-9d1-r-27820241631635.jpg "Western Blot: IRP2 Antibody (9D1-R) [NBP3-32483] - Western blot analysis of IRP2 on different lysates with Mouse anti-IRP2 antibody (NBP3-32483) at 1/2,000 dilution. Lane 1: Daudi cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: Mouse liver tissue lysate (40 ug/Lane) Lane 4: Mouse kidney tissue lysate (40 ug/Lane) Lane 5: Mouse heart tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32483) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Leptin/OB Antibody (002) [NBP2-89345] - Analysis of Human LEP expression on HeLa cells. The cells were treated, stained with purified anti-Human LEP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-002-Flow-Cytometry-NBP2-89345-img0001.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: Leptin/OB Antibody (002) [NBP2-89345] - Analysis of Human LEP expression on HeLa cells. The cells were treated, stained with purified anti-Human LEP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Leptin/OB Antibody (002) [NBP2-89345] - Staining of LEP in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-89345 (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Cytoplasm .](http://images.novusbio.com/fullsize/nbp2-89345_rabbit-monoclonal-leptin-ob-antibody-002-23520251129371.png)
![Western Blot: Carbonic Anhydrase VIII/CA8 Antibody (008) [NBP2-89517] - Lane A: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDa Observed band size:38 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-008-Western-Blot-NBP2-89517-img0001.jpg "Western Blot: Carbonic Anhydrase VIII/CA8 Antibody (008) [NBP2-89517] - Lane A: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDa Observed band size:38 kDa")
![Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody (008) [NBP2-89517] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg A549 Whole Cell Lysate0.5 uL anti-CA8 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-CA8 rabbit monoclonal antibody,at 1:150 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 37 kDa Observed band size: 37 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-008-Immunoprecipitation-NBP2-89517-img0002.jpg)
![Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP2-97978] - Immunochemical staining of human FBPase 1 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FBPase-1-Antibody-Immunohistochemistry-Paraffin-NBP2-97978-img0002.jpg "Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP2-97978] - Immunochemical staining of human FBPase 1 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP2-97978] - Immunochemical staining of human FBPase 1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FBPase-1-Antibody-Immunohistochemistry-Paraffin-NBP2-97978-img0001.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody (214) [NBP2-89460] - Staining of human TXN in human appendix with rabbit monoclonal antibody (1:200). Positive staining was localized to intestinal gland.](http://images.novusbio.com/fullsize/Thioredoxin-1-Antibody-214-Immunohistochemistry-Paraffin-NBP2-89460-img0002.jpg "Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody (214) [NBP2-89460] - Staining of human TXN in human appendix with rabbit monoclonal antibody (1:200). Positive staining was localized to intestinal gland.")
![Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody (214) [NBP2-89460] - Staining of human TXN in human colon with rabbit monoclonal antibody (1:200). Positive staining was localized to colonic gland.](http://images.novusbio.com/fullsize/Thioredoxin-1-Antibody-214-Immunohistochemistry-Paraffin-NBP2-89460-img0001.jpg)