| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92759-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04382-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38190-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB41442-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24950SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26497-50ul | |||
![Western Blot: MEKK1 Antibody [NBP3-02962] - Analysis of extracts of various cell lines, using MEKK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/MEKK1-Antibody-Western-Blot-NBP3-02962-img0004.jpg "Western Blot: MEKK1 Antibody [NBP3-02962] - Analysis of extracts of various cell lines, using MEKK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunocytochemistry/Immunofluorescence: MEKK1 Antibody [NBP3-02962] - Analysis of L929 cells using MEKK1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MEKK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-02962-img0001.jpg)
![Immunohistochemistry-Paraffin: MEKK1 Antibody [NBP3-02962] - Mouse kidney using MEKK1 antibody (NBP3-02962) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MEKK1-Antibody-Immunohistochemistry-Paraffin-NBP3-02962-img0005.jpg)
![Immunohistochemistry-Paraffin: MEKK1 Antibody [NBP3-02962] - Human liver using MAP3K1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MEKK1-Antibody-Immunohistochemistry-Paraffin-NBP3-02962-img0002.jpg)
![Immunohistochemistry-Paraffin: MEKK1 Antibody [NBP3-02962] - Rat liver using MAP3K1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MEKK1-Antibody-Immunohistochemistry-Paraffin-NBP3-02962-img0003.jpg)
![Western Blot: Catalase Antibody (7D2Y4) [NBP3-15357] - Western blot analysis of extracts of various cell lines, using Catalase Rabbit mAb (NBP3-15357) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Catalase-Antibody-7D2Y4-Western-Blot-NBP3-15357-img0002.jpg "Western Blot: Catalase Antibody (7D2Y4) [NBP3-15357] - Western blot analysis of extracts of various cell lines, using Catalase Rabbit mAb (NBP3-15357) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Catalase Antibody (7D2Y4) [NBP3-15357] - Immunohistochemistry of paraffin-embedded Mouse kidney using Catalase antibody (NBP3-15357) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Catalase-Antibody-7D2Y4-Immunohistochemistry-Paraffin-NBP3-15357-img0001.jpg)
![Western Blot: Catalase Antibody (7D2Y4) [Catalase] - Western blot analysis of lysates from Hep G2 cells using Catalase Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15357_rabbit-catalase-mab-7d2y4-52202517213587.jpg)
![Immunohistochemistry: Catalase Antibody (7D2Y4) [Catalase] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Catalase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15357_rabbit-catalase-mab-7d2y4-5220251728490.jpg)
![Immunohistochemistry: Catalase Antibody (7D2Y4) [Catalase] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Catalase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15357_rabbit-catalase-mab-7d2y4-52202517223975.jpg)
![Immunohistochemistry: Catalase Antibody (7D2Y4) [Catalase] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Catalase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15357_rabbit-catalase-mab-7d2y4-52202517172853.jpg)
![Western Blot: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Western blot analysis of extracts of Raji cells, using 5-Lipoxygenase Rabbit mAb (NBP3-16150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-5C5Z2-Western-Blot-NBP3-16150-img0004.jpg "Western Blot: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Western blot analysis of extracts of Raji cells, using 5-Lipoxygenase Rabbit mAb (NBP3-16150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Immunohistochemistry of paraffin-embedded mouse spleen using 5-Lipoxygenase Rabbit mAb (NBP3-16150) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-5C5Z2-Immunohistochemistry-Paraffin-NBP3-16150-img0002.jpg)
![Western Blot: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Western blot analysis of extracts of Rat lung, using 5-Lipoxygenase Rabbit mAb (NBP3-16150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-5C5Z2-Western-Blot-NBP3-16150-img0005.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Immunohistochemistry of paraffin-embedded human appendix using 5-Lipoxygenase Rabbit mAb (NBP3-16150) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-5C5Z2-Immunohistochemistry-Paraffin-NBP3-16150-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Confocal imaging of U-2 OS cells using 5-Lipoxygenase Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16150_rabbit-5-lipoxygenase-mab-5c5z2-5220251242536.jpg)
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Analysis of extracts of Mouse heart, using Acetyl-CoA Carboxylase alpha/ACACA Rabbit mAb (NBP3-15744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-5J4W7-Western-Blot-NBP3-15744-img0004.jpg "Western Blot: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Analysis of extracts of Mouse heart, using Acetyl-CoA Carboxylase alpha/ACACA Rabbit mAb (NBP3-15744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Mouse testis using Acetyl-CoA Carboxylase alpha/ACACA Rabbit mAb (NBP3-15744) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-5J4W7-Immunohistochemistry-Paraffin-NBP3-15744-img0002.jpg)
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Analysis of extracts of various cell lines, using Acetyl-CoA Carboxylase alpha/ACACA Rabbit mAb (NBP3-15744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-5J4W7-Western-Blot-NBP3-15744-img0003.jpg)
![Immunohistochemistry-Paraffin: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Rat kidney using Acetyl-CoA Carboxylase alpha/ACACA Rabbit mAb (NBP3-15744) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-5J4W7-Immunohistochemistry-Paraffin-NBP3-15744-img0001.jpg)
![Immunoprecipitation: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Analysis of 300ug extracts of HepG2 cells using 3ug Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-15744). Western blot was performed from the immunoprecipitate using Acetyl-CoA Carboxylase alpha/ACACA (NBP3-15744) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-5J4W7-Immunoprecipitation-NBP3-15744-img0005.jpg)
![Western Blot: CYP11A1 Antibody - Azide and BSA Free [NBP2-92759] - Western blot analysis of lysates from Rat testis, using CYP11A1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 18s.](http://images.novusbio.com/fullsize/nbp2-92759_rabbit-polyclonal-cyp11a1-antibody-4220251429061.jpg "Western Blot: CYP11A1 Antibody - Azide and BSA Free [NBP2-92759] - Western blot analysis of lysates from Rat testis, using CYP11A1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 18s.")
![Western Blot: CYP11A1 Antibody [NBP2-92759] - Western blot analysis of extracts of Mouse testis, using CYP11A1 antibody (NBP2-92759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Western-Blot-NBP2-92759-img0005.jpg)
![Western Blot: CYP11A1 Antibody - Azide and BSA Free [NBP2-92759] - Western blot analysis of various lysates using CYP11A1 Rabbit pAb at 1:800 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp2-92759_rabbit-polyclonal-cyp11a1-antibody-4220251430418.jpg)
![Immunocytochemistry/ Immunofluorescence: CYP11A1 Antibody - Azide and BSA Free [NBP2-92759] - Immunofluorescence analysis of paraffin-embedded human placenta using CYP11A1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92759_rabbit-polyclonal-cyp11a1-antibody-4220251430476.jpg)
![Immunohistochemistry: CYP11A1 Antibody - Azide and BSA Free [NBP2-92759] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using CYP11A1 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-92759_rabbit-polyclonal-cyp11a1-antibody-42202514275222.jpg)
![Western Blot: HDLBP Antibody [NBP3-04382] - Analysis of extracts of various cell lines, using HDLBP antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/HDLBP-Antibody-Western-Blot-NBP3-04382-img0004.jpg "Western Blot: HDLBP Antibody [NBP3-04382] - Analysis of extracts of various cell lines, using HDLBP antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunocytochemistry/Immunofluorescence: HDLBP Antibody [NBP3-04382] - Analysis of L929 cells using HDLBP Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HDLBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04382-img0005.jpg)
![Immunohistochemistry-Paraffin: HDLBP Antibody [NBP3-04382] - Paraffin-embedded mouse liver using HDLBP antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HDLBP-Antibody-Immunohistochemistry-Paraffin-NBP3-04382-img0003.jpg)
![Immunohistochemistry-Paraffin: HDLBP Antibody [NBP3-04382] - Paraffin-embedded rat liver using HDLBP antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HDLBP-Antibody-Immunohistochemistry-Paraffin-NBP3-04382-img0001.jpg)
![Immunohistochemistry-Paraffin: HDLBP Antibody [NBP3-04382] - Paraffin-embedded human liver using HDLBP antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HDLBP-Antibody-Immunohistochemistry-Paraffin-NBP3-04382-img0002.jpg)
![Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-101220241252813.jpg "Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Human colon using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-101220241233823.jpg)
![Western Blot: Aspartate Aminotransferase Antibody [NBP3-38190] - Western blot analysis of various lysates using Aspartate Aminotransferase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-1012202411592816.jpg)
![Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Rat heart using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-10122024120650.jpg)
![Immunocytochemistry/ Immunofluorescence: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunofluorescence analysis of U2OS cells using Aspartate Aminotransferase Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-101220241243928.jpg)
![Western Blot: BMAL1 Antibody [NB100-129] - Detection of BMAL in 3T3/L1 lysates.](http://images.novusbio.com/fullsize/BMAL1-Antibody-Western-Blot-NB100-129-img0003.jpg "Western Blot: BMAL1 Antibody [NB100-129] - Detection of BMAL in 3T3/L1 lysates.")
![Simple Western: BMAL1 Antibody [NB100-129] - Image shows a specific band for BMAL1 in 0.5 mg/mL of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/BMAL1-Antibody-Simple-Western-NB100-129-img0004.jpg)
![Western Blot: Nogo B receptor Antibody [NBP2-24950] - Western blot analysis of Nogo-Breceptor in A) human, B) mouse and C) rat brain tissue lysate using NBP2-24950 at 2ug/ml.](http://images.novusbio.com/fullsize/Nogo-B-receptor-Antibody-Western-Blot-NBP2-24950-img0002.jpg "Western Blot: Nogo B receptor Antibody [NBP2-24950] - Western blot analysis of Nogo-Breceptor in A) human, B) mouse and C) rat brain tissue lysate using NBP2-24950 at 2ug/ml.")
![Immunoprecipitation: ATF4 Antibody (9E1) [NBP3-26497] - Immunoprecipitating ATF4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26497 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26497 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-26220241322155.jpg "Immunoprecipitation: ATF4 Antibody (9E1) [NBP3-26497] - Immunoprecipitating ATF4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26497 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26497 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).")
![Immunohistochemistry: ATF4 Antibody (9E1) [NBP3-26497] - Image of ATF4 Antibody (9E1) diluted at 1:160 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-26220241510616.jpg)
![Western Blot: ATF4 Antibody (9E1) [NBP3-26497] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate. All lanes: ATF4 Antibody at 1.6ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39 KDa Observed band size: 50 KDa](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-282202411472134.jpg)
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (9E1) [NBP3-26497] - Staining of HepG2 cells with ATF4 Antibody (9E1) at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-28220241312211.jpg)
![Flow Cytometry: ATF4 Antibody (9E1) [NBP3-26497] - Overlay histogram showing Hela cells stained with ATF4 Antibody (9E1) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-282202414312786.jpg)