| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22212-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73362 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73344 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76810SS | |||
| Azide and BSA Free | NBP2-80713 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75977SS | |||
| Azide and BSA Free | NBP2-80764 | |||
![Western Blot: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis using PI 3 Kinase p85 alpha mAb against human PI 3 Kinase p85 alpha recombinant protein. (Expected MW is 53.4 kDa)](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Western-Blot-NBP2-22212-img0007.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis using PI 3 Kinase p85 alpha mAb against human PI 3 Kinase p85 alpha recombinant protein. (Expected MW is 53.4 kDa)")
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of HepG2 cells using PI 3 Kinase p85 alpha mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Immunocytochemistry-NBP2-22212-img0009.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of rectum cancer tissues using PI 3 Kinase p85 alpha mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Immunohistochemistry-Paraffin-NBP2-22212-img0005.jpg)
![Flow Cytometry: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of NIH3T3 cells using PI 3 Kinase p85 alpha mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Flow-Cytometry-NBP2-22212-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of HeLa cells using PI 3 Kinase p85 alpha mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Immunocytochemistry-NBP2-22212-img0008.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of cervical cancer tissues using PI 3 Kinase p85 alpha mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Immunohistochemistry-Paraffin-NBP2-22212-img0004.jpg)
![ELISA: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-ELISA-NBP2-22212-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NBP2-16400] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 protein stained by FACL4 antibody [N1N3] diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16400-img0002.jpg "Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NBP2-16400] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 protein stained by FACL4 antibody [N1N3] diluted at 1:200. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16400] - Paraffin-embedded mouse kidney. FACL4 antibody [N1N3] diluted at 1:500.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16400-img0011.jpg)
![Western Blot: FACL4 Antibody [NBP2-16400] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/FACL4-Antibody-Western-Blot-NBP2-16400-img0001.jpg)
![Western Blot: FACL4 Antibody [NBP2-16400] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-Western-Blot-NBP2-16400-img0007.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16400] - Paraffin-embedded rat duodenum. FACL4 antibody [N1N3] diluted at 1:500](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16400-img0010.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-261120251827016.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-261120251823622.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-2611202518204710.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-2611202518422918.jpg)
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Analysis of methanol-fixed A431, using LDHA antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19320-img0015.jpg "Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Analysis of methanol-fixed A431, using LDHA antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Mouse liver. LDHA stained by LDHA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-Immunohistochemistry-Paraffin-NBP2-19320-img0017.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Human lung papillory adenocarcinoma, using LDHA antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-Antibody-Immunohistochemistry-Paraffin-NBP2-19320-img0002.jpg)
![Immunohistochemistry-Paraffin: HADH Antibody [NBP2-16776] - HADH antibody detects HADH protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. HADH antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HADH-Antibody-Immunohistochemistry-Paraffin-NBP2-16776-img0009.jpg "Immunohistochemistry-Paraffin: HADH Antibody [NBP2-16776] - HADH antibody detects HADH protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. HADH antibody dilution: 1:500.")
![Western Blot: HADH Antibody [NBP2-16776] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE](http://images.novusbio.com/fullsize/HADH-Antibody-Western-Blot-NBP2-16776-img0001.jpg)
![Immunohistochemistry-Paraffin: HADH Antibody [NBP2-16776] - HADH antibody detects HADH protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. HADH antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HADH-Antibody-Immunohistochemistry-Paraffin-NBP2-16776-img0006.jpg)
![Immunoprecipitation: HADH Antibody [NBP2-16776] - Immunoprecipitation of HADH protein from HepG2 whole cell extracts using 5 ug of HADH antibody. Western blot analysis was performed using HADH antibody.](http://images.novusbio.com/fullsize/HADH-Antibody-Immunoprecipitation-NBP2-16776-img0008.jpg)
![Western Blot: PGM3 Antibody (1A11) [NBP2-03734] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PGM3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Western-Blot-NBP2-03734-img0009.jpg "Western Blot: PGM3 Antibody (1A11) [NBP2-03734] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PGM3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Human prostate tissue using anti-PGM3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-03734-img0008.jpg)
![Flow Cytometry: PGM3 Antibody (1A11) [NBP2-03734] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PGM3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Flow-Cytometry-NBP2-03734-img0001.jpg)
![Western Blot: PGM3 Antibody (1A11) [NBP2-03734] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGM3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGM3.](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Western-Blot-NBP2-03734-img0002.jpg)
![Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PGM3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-03734-img0003.jpg)
![Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PGM3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-03734-img0004.jpg)
![Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Human Kidney tissue using anti-PGM3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-03734-img0005.jpg)
![Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Human liver tissue using anti-PGM3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-03734-img0006.jpg)
![Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Human pancreas tissue using anti-PGM3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-03734-img0007.jpg)
![Western Blot: PFKP Antibody (OTI1D6) [NBP2-01539] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cellsand PFKP-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-PFKP monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.](http://images.novusbio.com/fullsize/PFKP-Antibody-OTI1D6-Western-Blot-NBP2-01539-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PFKP Antibody (OTI1D6) [NBP2-01539] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cellsand PFKP-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-PFKP monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PFKP Antibody (OTI1D6) [NBP2-01539] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PFKP.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Immunocytochemistry-Immunofluorescence-NBP2-01539-img0012.jpg)
![Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Human endometrium tissue using anti-PFKP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01539-img0009.jpg)
![Flow Cytometry: PFKP Antibody (1D6) [NBP2-01539] - Analysis of Jurkat cells, using anti-PFKP antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Flow-Cytometry-NBP2-01539-img0001.jpg)
![Western Blot: PFKP Antibody (1D6) [NBP2-01539] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFKP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFKP.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Western-Blot-NBP2-01539-img0002.jpg)
![Western Blot: PFKP Antibody (1D6) [NBP2-01539] Analysis of extracts (35ug) from 9 different cell lines by using anti-PFKP monoclonal antibody.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Western-Blot-NBP2-01539-img0011.jpg)
![Western Blot: PFKP Antibody (OTI1D6) [NBP2-01539] - Analysis of extracts (10ug) from 9 Human tissues by using anti-PFKP monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).](http://images.novusbio.com/fullsize/PFKP-Antibody-OTI1D6-Western-Blot-NBP2-01539-img0014.jpg)
![Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PFKP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01539-img0003.jpg)
![Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PFKP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01539-img0004.jpg)
![Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PFKP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01539-img0005.jpg)
![Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PFKP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01539-img0006.jpg)
![Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PFKP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01539-img0007.jpg)
![Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PFKP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01539-img0008.jpg)
![PFKP Antibody (OTI1D6)[NBP2-01539]Simple Western� analysis of endogenous protein DGKA from Jurkat lysates (0.2 mg/mL) using DGKA Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-01539_mouse-pfkp-mab-oti1d6-simple-western-131120251236358.jpg%253fstore=america&image-type=image)
![Immunoprecipitation: FACL4 Antibody (3I8D10) [NBP3-16012] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug FACL4 antibody (NBP3-16012). Western blot was performed from the immunoprecipitate using FACL4 antibody (NBP3-16012) at a dilition of 1:2000.](http://images.novusbio.com/fullsize/FACL4-Antibody-3I8D10-Immunoprecipitation-NBP3-16012-img0002.jpg "Immunoprecipitation: FACL4 Antibody (3I8D10) [NBP3-16012] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug FACL4 antibody (NBP3-16012). Western blot was performed from the immunoprecipitate using FACL4 antibody (NBP3-16012) at a dilition of 1:2000.")
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-5220251718945.jpg)
![Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of various lysates using FACL4 Rabbit mAb at 1:58000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517184534.jpg)
![Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of lysates from Rat liver using FACL4 Rabbit mAb at 1:58000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517202918.jpg)
![Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of lysates from Hep G2 cells using FACL4 Rabbit mAb at 1:58000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517184563.jpg)
![Immunocytochemistry/ Immunofluorescence: FACL4 Antibody (4W3T9) [FACL4] - Confocal imaging of Hep G2 cells using FACL4 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517172885.jpg)
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Rat brain using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517213510.jpg)
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517213524.jpg)
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human kidney using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517172887.jpg)
![Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PKM2-Antibody-Western-Blot-NBP1-82487-img0012.jpg "Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody [NBP1-82487] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PKM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82487-img0009.jpg)
![Immunohistochemistry-Paraffin: PKM2 Antibody [NBP1-82487] - Staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/PKM2-Antibody-Immunohistochemistry-Paraffin-NBP1-82487-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PKM antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PKM2-Antibody-Western-Blot-NBP1-82487-img0011.jpg)
![Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751] - Analysis in human liver and skeletal muscle tissues using NBP1-85751 antibody. Corresponding ENTPD5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD39L4-ENTPD5-Antibody-Immunohistochemistry-Paraffin-NBP1-85751-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751] - Analysis in human liver and skeletal muscle tissues using NBP1-85751 antibody. Corresponding ENTPD5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CD39L4-ENTPD5-Antibody-Immunohistochemistry-Paraffin-NBP1-85751-img0012.jpg)
![Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751] - Staining of human kidney shows strong cytoplasmic positivity in cells in proximal tubules.](http://images.novusbio.com/fullsize/CD39L4-ENTPD5-Antibody-Immunohistochemistry-Paraffin-NBP1-85751-img0013.jpg)
![Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/CD39L4-ENTPD5-Antibody-Immunohistochemistry-Paraffin-NBP1-85751-img0014.jpg)
![Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CD39L4-ENTPD5-Antibody-Immunohistochemistry-Paraffin-NBP1-85751-img0015.jpg)
![Simple Western: CD39L4/ENTPD5 Antibody [NBP1-85751] - Simple Western lane view shows a specific band for CD39L4/ENTPD5 in 0.2 mg/ml of h. Liver lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CD39L4-ENTPD5-Antibody-Simple-Western-NBP1-85751-img0006.jpg)
![Simple Western: CD39L4/ENTPD5 Antibody [NBP1-85751] - Electropherogram image of the corresponding Simple Western lane view. CD39L4/ENTPD5 antibody was used at 1:25 dilution on h. Liver lysate(s) respectively.](http://images.novusbio.com/fullsize/CD39L4-ENTPD5-Antibody-Simple-Western-NBP1-85751-img0008.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Liver tissue](http://images.novusbio.com/fullsize/nbp1-85751_rabbit-polyclonal-cd39l4-entpd5-antibody-24420259374134.jpg)
![Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (NBP3-13666) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-HL229-Western-Blot-NBP3-13666-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (NBP3-13666) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FACL4 Antibody (HL229) [NBP3-13666] - FACL4 antibody [HL229] detects FACL4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 stained by FACL4 antibody [HL229] (NBP3-13666) diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/FACL4-Antibody-HL229-Immunocytochemistry-Immunofluorescence-NBP3-13666-img0002.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody (HL229) [NBP3-13666] - FACL4 antibody [HL229] detects FACL4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. FACL4 stained by FACL4 antibody [HL229] (NBP3-13666) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/FACL4-Antibody-HL229-Immunohistochemistry-Paraffin-NBP3-13666-img0003.jpg)
![Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (NBP3-13666) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-HL229-Western-Blot-NBP3-13666-img0004.jpg)
![Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (NBP3-13666) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-HL229-Western-Blot-NBP3-13666-img0005.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody (HL229) [NBP3-13666] - FACL4 antibody [HL44] detects FACL4 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.FACL4 stained by FACL4 antibody [HL44] (NBP3-13666) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13666_rabbit-facl4-mab-hl229-910202419593532.jpg)
![Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Drosophila tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (NBP3-13666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/nbp3-13666_rabbit-facl4-mab-hl229-910202420122075.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13666_rabbit-facl4-mab-hl229-1610202419414568.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyt(TM) HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm and nuclei.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Immunohistochemistry-Paraffin-NBP2-76810-img0003.jpg "Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyt(TM) HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm and nuclei.")
![Western Blot: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 antibody in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Western-Blot-NBP2-76810-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLight(TM) 557-conjugated Anti-Rabbit IgG secondary antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm and nuclei.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Immunocytochemistry-Immunofluorescence-NBP2-76810-img0002.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - Human Jurkat T Cell Leukemia Cell Line was stained with Rabbit anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Flow-Cytometry-NBP2-76810-img0001.jpg)
![Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg)